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The map label for this gene is amtB [C]

Identifier: 74317719

GI number: 74317719

Start: 1792571

End: 1793773

Strand: Reverse

Name: amtB [C]

Synonym: Tbd_1701

Alternate gene names: 74317719

Gene position: 1793773-1792571 (Counterclockwise)

Preceding gene: 74317720

Following gene: 74317713

Centisome position: 61.65

GC content: 66.25

Gene sequence:

>1203_bases
ATGGAAGCTTTGCAATCTGGCAGCGACGTCCTCTTCGTCCTGCTCGGCGGAATCATGGTGCTCGCGATGCACGCGGGGTT
CGCCTTTCTCGAGCTCGGCACGGTGCGCAAGAAGAACCAGGTCAACGCCCTGGTCAAGATCTTGACCGACTTCTCGGTCT
CGACAATCGCCTATTTCTTCATCGGCTACGGTATCGCGTACGGCGTGAGCTTCTTTTCGGGCGCGGCAGCCTTGTCGGCC
CAGAACGGCTACGCGCTGGTCAAGTTCTTCTTTCTGCTCACCTTTGCGGCGGCCATCCCCGCGATCGTATCCGGCGGCAT
CGCCGAGCGCGCGCGCTTCAATCCGCAGCTCGCCGCGACCTTCGCGCTCGTCGGCTTTGTCTATCCCTTCTTCGAAGGCA
TGGTCTGGAACGGGAATTATGGCGTTCAGGACTGGCTTGCCGCGGCGACCGGCGCCAAATTCCACGACTTCGCCGGGTCG
GTCGTCGTGCATGCGGTCGGCGGCTGGATCGCGCTGCCGGCGGTGCTCCTGCTCGGCGCGCGGCGCGGGCGCTACAAGGG
CGACGGGATGATGTCGGCACACCCGCCTTCGAACATCCCGTTTCTCGCGCTGGGGGCTTGGATCCTGACCGTCGGCTGGT
TCGGCTTCAACGTGATGTCCGCCCAGCTCATCGCCGCGGTGTCGGGTCTGGTCGCGGTCAATTCGCTCATGGCGATGGTC
GGGGGAACGCTGGCGGCGCTGATTGTCGGGCGCAACGACCCGGGTTTCATCCACAACGGTCCGCTCGCAGGCCTTGTCGC
CGTGTGCGCCGGTTCGGATCTCATGCACCCTCTCGGAGCGCTTGCGACCGGGGCGATCGCCGGCGCGCTGTTCGTGGGCA
TGTTCACGGTCACGCAGAACCGATGGAAGATCGATGACGTGCTCGGGGTGTGGCCGCTTCACGGTCTCTGCGGAGCGTGG
GGCGGTGTCGCGGCGGGAATCTTCGGCGCACAGGCGCTCGGCGGGCTCGGCGGCGTAAACCTCGGGGCGCAACTCGTCGG
CACGCTCGGCGGCGTGGGGATCGCGCTTGCGGGTGGCGCGCTCGTCTACGGTGCGCTGAAGCTCGGCGTCGGTCTGCGGC
TCGATCCGGAGGCCGAGTTCACCGGCGCCGACCTCAGCATCCACAAGGTATCGGCGACCGCCGAGCGGGAGACGAACTGG
TAA

Upstream 100 bases:

>100_bases
GCAGTGCGCACGGACTTAGGGCGTTCTTCCCGCCCCGTAAAGGCCCATCCAGGCTGGAATGCCGCTTGCTTCTCTCGCCC
CCATTCACTTGGGGGAAAGC

Downstream 100 bases:

>100_bases
GCGTCCGTCAGTCGGCAGCCTGCTTCTGTAGCTGACGTAGATGCCGGTTTCGCTCGGCGCGGAGCCGATCGAGCTGCGCG
TCGATCTGCACGACGTCGGG

Product: putative ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MEALQSGSDVLFVLLGGIMVLAMHAGFAFLELGTVRKKNQVNALVKILTDFSVSTIAYFFIGYGIAYGVSFFSGAAALSA
QNGYALVKFFFLLTFAAAIPAIVSGGIAERARFNPQLAATFALVGFVYPFFEGMVWNGNYGVQDWLAAATGAKFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYKGDGMMSAHPPSNIPFLALGAWILTVGWFGFNVMSAQLIAAVSGLVAVNSLMAMV
GGTLAALIVGRNDPGFIHNGPLAGLVAVCAGSDLMHPLGALATGAIAGALFVGMFTVTQNRWKIDDVLGVWPLHGLCGAW
GGVAAGIFGAQALGGLGGVNLGAQLVGTLGGVGIALAGGALVYGALKLGVGLRLDPEAEFTGADLSIHKVSATAERETNW

Sequences:

>Translated_400_residues
MEALQSGSDVLFVLLGGIMVLAMHAGFAFLELGTVRKKNQVNALVKILTDFSVSTIAYFFIGYGIAYGVSFFSGAAALSA
QNGYALVKFFFLLTFAAAIPAIVSGGIAERARFNPQLAATFALVGFVYPFFEGMVWNGNYGVQDWLAAATGAKFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYKGDGMMSAHPPSNIPFLALGAWILTVGWFGFNVMSAQLIAAVSGLVAVNSLMAMV
GGTLAALIVGRNDPGFIHNGPLAGLVAVCAGSDLMHPLGALATGAIAGALFVGMFTVTQNRWKIDDVLGVWPLHGLCGAW
GGVAAGIFGAQALGGLGGVNLGAQLVGTLGGVGIALAGGALVYGALKLGVGLRLDPEAEFTGADLSIHKVSATAERETNW
>Mature_400_residues
MEALQSGSDVLFVLLGGIMVLAMHAGFAFLELGTVRKKNQVNALVKILTDFSVSTIAYFFIGYGIAYGVSFFSGAAALSA
QNGYALVKFFFLLTFAAAIPAIVSGGIAERARFNPQLAATFALVGFVYPFFEGMVWNGNYGVQDWLAAATGAKFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYKGDGMMSAHPPSNIPFLALGAWILTVGWFGFNVMSAQLIAAVSGLVAVNSLMAMV
GGTLAALIVGRNDPGFIHNGPLAGLVAVCAGSDLMHPLGALATGAIAGALFVGMFTVTQNRWKIDDVLGVWPLHGLCGAW
GGVAAGIFGAQALGGLGGVNLGAQLVGTLGGVGIALAGGALVYGALKLGVGLRLDPEAEFTGADLSIHKVSATAERETNW

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=31.0262529832936, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=419, Percent_Identity=29.8329355608592, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=29.6983758700696, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=430, Percent_Identity=27.4418604651163, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=439, Percent_Identity=28.246013667426, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=25.0596658711217, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325396, Length=419, Percent_Identity=24.582338902148, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=404, Percent_Identity=27.970297029703, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=30.8056872037915, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41095; Mature: 41095

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure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HHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]