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The map label for this gene is mltD [H]

Identifier: 74317684

GI number: 74317684

Start: 1749531

End: 1751036

Strand: Direct

Name: mltD [H]

Synonym: Tbd_1666

Alternate gene names: 74317684

Gene position: 1749531-1751036 (Clockwise)

Preceding gene: 74317683

Following gene: 74317690

Centisome position: 60.13

GC content: 66.47

Gene sequence:

>1506_bases
GTGCCCGGAATCAAATTCACCCGTATCGGTGCAGCGCGCCTCCCTCTCGGCCGGCTCGGATTGGCGGCCCTGATGTTCTG
GGCAAGCCATCACAGCCTGGCGACGGAACCCCGACAAGGCATCCACACACTCGAATGGAACGGCGGCAGTGATAACGTGC
CCGCTGCCGACGAGGTGCCCGCGATCGCCCTTGAAGCTGGCACGCGCGACGTCTGGCAGCGTGTCCGCGACGGTTTCCGC
ATCGACGACCAGGCGGCGCCGAACCCGCTCATCGCTGTTCACGAATCCTGGTACGCAGCCCGGCCCGAACACGTGCGTCG
CGTCGTCGAGCGCGCGCGCCGCTATCTTTTCTATATCGTCGAAGAGGTCGAGCGGCGCGGCATGCCGATGGAAATCGCGC
TGCTGCCCATGATCGAGAGCGCGTTCGACCCCGGCGCGCTCTCGCCCTCCGCGGCGTCCGGAATCTGGCAGTTCATTCCT
TCCACCGGACGTCTGTACGGCCTCAGGCAGGACAAATGGTACGACGGCCGCCTCGATTTCACCGCCGCGACCGGGGCCGC
ACTGGACTACCTCGGCAAGCTCTATCTCGACTTCGGTGACTGGCAACTCGCGCTCGCGGCGTACAACTGCGGCGAAGGCT
GCGTCGGTCGCGCGATCCAGCGGAACGTCCAGCAAGGCCTTCCAACCGACTACGCAAGCCTCGCGCTGCCGAACGAGACG
CGTCACTACGTCCCCAAGCTGCTCGCGATCCGCAACCTGGTGCGCAATCCCGAGCAATACGGCATGACGATAGACGGGCT
TCCAAACGAGCCTTATTTCAACCTGGTGAGCGTGAGCGCCAGCATGGATCTGCATTCGGCCGCGCGGCTGGCGGGCATGA
CCATCGAGGAGTTCGTCGCGCTCAACGCGGCCTTTCCGCGCAAGCTGATCCGCTCCGACACGCCGGTCAGCCTGCTCGTT
CCGGTCGACCGCGCCGACCGTTTCCAGCGCAATCTGGATGCCGGCGACTGGGACAGTTGGCAACCCTACGCCGCACGCAA
GGGCGAGCGGCCCGAGGAGATCGCGCGCCGCTTCAAGGTCAGCGTGGCACGACTCGCCGAACACAACCAGTTCAAGCTCA
GACGCGGCAGGCTGGCACGCGCACAGACCGTGCTGGTGCCGGTCAAACGTCGTGACACCGGTATCGCCGCGAACCAGTCG
GGCAGTCGTCGTCCTGCGCCGACCGAACACGAAGTCAAGGCTGGCGAGACGCTCTACGGCGTCGCGCGGCGCTACGGCTT
GAGCGCCCGCGAATTGGCGGACGCCAATCCGGGGGTCGGGGCAAGACTCAGGCCGGGGCAGGTCCTGCAACTGGCAATCC
CGGTTTCTTCGCCGCGCGAGACGGCCCCATTGCGACCGCGCAAGACGGGCGCTCGGGGTGCCGCCCTGCCGTCACACTAC
GCCCCGAACCGCGGCGACACGCTTCTCACGATCGCCCGCCGCTTCGATATCAGCATGGTCAGGTGA

Upstream 100 bases:

>100_bases
CGGTCTTCGGCGCGATCCGTGCGGCCAAGGACCGGTGGGACGGTTGACTGCCCGGCGGACGCCCCTTAGGATGGAGCCTG
CTTGAGTACGGGAGACCCAC

Downstream 100 bases:

>100_bases
CGCGAGGGTTCGCGTCGCTCCTCGATTCTCGGGGCTGACCGTTCCGGCGCGTTCCGTCCGATCCAGGCCGCGGCTAAACC
ACCTATCCCGGACCCGCCCC

Product: peptidoglycan-binding protein LysM

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MPGIKFTRIGAARLPLGRLGLAALMFWASHHSLATEPRQGIHTLEWNGGSDNVPAADEVPAIALEAGTRDVWQRVRDGFR
IDDQAAPNPLIAVHESWYAARPEHVRRVVERARRYLFYIVEEVERRGMPMEIALLPMIESAFDPGALSPSAASGIWQFIP
STGRLYGLRQDKWYDGRLDFTAATGAALDYLGKLYLDFGDWQLALAAYNCGEGCVGRAIQRNVQQGLPTDYASLALPNET
RHYVPKLLAIRNLVRNPEQYGMTIDGLPNEPYFNLVSVSASMDLHSAARLAGMTIEEFVALNAAFPRKLIRSDTPVSLLV
PVDRADRFQRNLDAGDWDSWQPYAARKGERPEEIARRFKVSVARLAEHNQFKLRRGRLARAQTVLVPVKRRDTGIAANQS
GSRRPAPTEHEVKAGETLYGVARRYGLSARELADANPGVGARLRPGQVLQLAIPVSSPRETAPLRPRKTGARGAALPSHY
APNRGDTLLTIARRFDISMVR

Sequences:

>Translated_501_residues
MPGIKFTRIGAARLPLGRLGLAALMFWASHHSLATEPRQGIHTLEWNGGSDNVPAADEVPAIALEAGTRDVWQRVRDGFR
IDDQAAPNPLIAVHESWYAARPEHVRRVVERARRYLFYIVEEVERRGMPMEIALLPMIESAFDPGALSPSAASGIWQFIP
STGRLYGLRQDKWYDGRLDFTAATGAALDYLGKLYLDFGDWQLALAAYNCGEGCVGRAIQRNVQQGLPTDYASLALPNET
RHYVPKLLAIRNLVRNPEQYGMTIDGLPNEPYFNLVSVSASMDLHSAARLAGMTIEEFVALNAAFPRKLIRSDTPVSLLV
PVDRADRFQRNLDAGDWDSWQPYAARKGERPEEIARRFKVSVARLAEHNQFKLRRGRLARAQTVLVPVKRRDTGIAANQS
GSRRPAPTEHEVKAGETLYGVARRYGLSARELADANPGVGARLRPGQVLQLAIPVSSPRETAPLRPRKTGARGAALPSHY
APNRGDTLLTIARRFDISMVR
>Mature_500_residues
PGIKFTRIGAARLPLGRLGLAALMFWASHHSLATEPRQGIHTLEWNGGSDNVPAADEVPAIALEAGTRDVWQRVRDGFRI
DDQAAPNPLIAVHESWYAARPEHVRRVVERARRYLFYIVEEVERRGMPMEIALLPMIESAFDPGALSPSAASGIWQFIPS
TGRLYGLRQDKWYDGRLDFTAATGAALDYLGKLYLDFGDWQLALAAYNCGEGCVGRAIQRNVQQGLPTDYASLALPNETR
HYVPKLLAIRNLVRNPEQYGMTIDGLPNEPYFNLVSVSASMDLHSAARLAGMTIEEFVALNAAFPRKLIRSDTPVSLLVP
VDRADRFQRNLDAGDWDSWQPYAARKGERPEEIARRFKVSVARLAEHNQFKLRRGRLARAQTVLVPVKRRDTGIAANQSG
SRRPAPTEHEVKAGETLYGVARRYGLSARELADANPGVGARLRPGQVLQLAIPVSSPRETAPLRPRKTGARGAALPSHYA
PNRGDTLLTIARRFDISMVR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=398, Percent_Identity=33.1658291457286, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55574; Mature: 55443

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIKFTRIGAARLPLGRLGLAALMFWASHHSLATEPRQGIHTLEWNGGSDNVPAADEVP
CCCCEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEECCCCCCCCCCCCCC
AIALEAGTRDVWQRVRDGFRIDDQAAPNPLIAVHESWYAARPEHVRRVVERARRYLFYIV
EEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHH
EEVERRGMPMEIALLPMIESAFDPGALSPSAASGIWQFIPSTGRLYGLRQDKWYDGRLDF
HHHHHCCCCEEEEHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCEEEE
TAATGAALDYLGKLYLDFGDWQLALAAYNCGEGCVGRAIQRNVQQGLPTDYASLALPNET
EEHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHEECCCCH
RHYVPKLLAIRNLVRNPEQYGMTIDGLPNEPYFNLVSVSASMDLHSAARLAGMTIEEFVA
HHHHHHHHHHHHHHCCHHHHCEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHHHH
LNAAFPRKLIRSDTPVSLLVPVDRADRFQRNLDAGDWDSWQPYAARKGERPEEIARRFKV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHH
SVARLAEHNQFKLRRGRLARAQTVLVPVKRRDTGIAANQSGSRRPAPTEHEVKAGETLYG
HHHHHHCCCHHHHHHCCHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHH
VARRYGLSARELADANPGVGARLRPGQVLQLAIPVSSPRETAPLRPRKTGARGAALPSHY
HHHHHCCCHHHHCCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
APNRGDTLLTIARRFDISMVR
CCCCCCEEEEEEHHCCCCCCC
>Mature Secondary Structure 
PGIKFTRIGAARLPLGRLGLAALMFWASHHSLATEPRQGIHTLEWNGGSDNVPAADEVP
CCCEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEECCCCCCCCCCCCCC
AIALEAGTRDVWQRVRDGFRIDDQAAPNPLIAVHESWYAARPEHVRRVVERARRYLFYIV
EEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHH
EEVERRGMPMEIALLPMIESAFDPGALSPSAASGIWQFIPSTGRLYGLRQDKWYDGRLDF
HHHHHCCCCEEEEHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCEEEE
TAATGAALDYLGKLYLDFGDWQLALAAYNCGEGCVGRAIQRNVQQGLPTDYASLALPNET
EEHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHEECCCCH
RHYVPKLLAIRNLVRNPEQYGMTIDGLPNEPYFNLVSVSASMDLHSAARLAGMTIEEFVA
HHHHHHHHHHHHHHCCHHHHCEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHHHH
LNAAFPRKLIRSDTPVSLLVPVDRADRFQRNLDAGDWDSWQPYAARKGERPEEIARRFKV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHH
SVARLAEHNQFKLRRGRLARAQTVLVPVKRRDTGIAANQSGSRRPAPTEHEVKAGETLYG
HHHHHHCCCHHHHHHCCHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHH
VARRYGLSARELADANPGVGARLRPGQVLQLAIPVSSPRETAPLRPRKTGARGAALPSHY
HHHHHCCCHHHHCCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
APNRGDTLLTIARRFDISMVR
CCCCCCEEEEEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]