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The map label for this gene is gloB

Identifier: 74317683

GI number: 74317683

Start: 1748707

End: 1749477

Strand: Direct

Name: gloB

Synonym: Tbd_1665

Alternate gene names: 74317683

Gene position: 1748707-1749477 (Clockwise)

Preceding gene: 74317677

Following gene: 74317684

Centisome position: 60.1

GC content: 67.06

Gene sequence:

>771_bases
ATGTTGACGCTGATTCCCCTGCCCGCGTTCGAGGACAACTACATCTGGGTGTGGCACGACGCGAAATACGCGGTCGCGGT
CGACCCCGGTGACCCTGCCGTGCTTTCGACCTATCTCGACAGCCGCGGACTCGCCCTCGCCGCGGTGCTCGTGACCCACC
ATCACCGCGACCATACTGGCGGCAACACCTGGCTGCGTCAACGCTATAACTGTGCCATCTACGCGCCCGACAACCCCCGT
ATCCCGGCGGTCAGTCATGTCGTGCGCGGCGGCGATTCGGTGGCGGTTCCCGAACTCGGCCTCGCGCTCGCCGTGCTCGC
GACGCCCGGCCATACGCTCGACCACGTCAGCTACGTCGGCAACGGGCATCTCTTCTGCGGTGATACGCTCTTCGGCTGCG
GTTGCGGCAAGCTGTTCGAGGGCGACGCAGCGATGATGTCGGCGAGTCTCGATGCGCTCGCCGCCTTGCCGCCCACAACG
CGCGTCTGCTGCGCGCACGAATATACCCTCAGCAACATCGACTTTGCCAAAACCATCGACGGCGCGAACCCCGCCCTGCT
CGAACGCGAGCGCATCGACCGCGCCGCACGCGCCCAAAACCGGCCGACCCTGCCGTCGACGCTCGCGCTCGAACGCACGA
CGAACCCGTTTTTGCGCTTCCACGACGCCGACATGCGCGCTTTCGCGGTCGGCGAACTCGGCAGCCCGGATCCGGGTCCC
GCCACGGTCTTCGGCGCGATCCGTGCGGCCAAGGACCGGTGGGACGGTTGA

Upstream 100 bases:

>100_bases
GCTGCTCGCGGAAGAGCAGATGCTGTCCCAACGGCGTGTCGAACCATCCGGCGTTCAGCTTGGATAACATAGCCCTTCCT
CCTGTCTCTTGCAGTCCATC

Downstream 100 bases:

>100_bases
CTGCCCGGCGGACGCCCCTTAGGATGGAGCCTGCTTGAGTACGGGAGACCCACGTGCCCGGAATCAAATTCACCCGTATC
GGTGCAGCGCGCCTCCCTCT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLTLIPLPAFEDNYIWVWHDAKYAVAVDPGDPAVLSTYLDSRGLALAAVLVTHHHRDHTGGNTWLRQRYNCAIYAPDNPR
IPAVSHVVRGGDSVAVPELGLALAVLATPGHTLDHVSYVGNGHLFCGDTLFGCGCGKLFEGDAAMMSASLDALAALPPTT
RVCCAHEYTLSNIDFAKTIDGANPALLERERIDRAARAQNRPTLPSTLALERTTNPFLRFHDADMRAFAVGELGSPDPGP
ATVFGAIRAAKDRWDG

Sequences:

>Translated_256_residues
MLTLIPLPAFEDNYIWVWHDAKYAVAVDPGDPAVLSTYLDSRGLALAAVLVTHHHRDHTGGNTWLRQRYNCAIYAPDNPR
IPAVSHVVRGGDSVAVPELGLALAVLATPGHTLDHVSYVGNGHLFCGDTLFGCGCGKLFEGDAAMMSASLDALAALPPTT
RVCCAHEYTLSNIDFAKTIDGANPALLERERIDRAARAQNRPTLPSTLALERTTNPFLRFHDADMRAFAVGELGSPDPGP
ATVFGAIRAAKDRWDG
>Mature_256_residues
MLTLIPLPAFEDNYIWVWHDAKYAVAVDPGDPAVLSTYLDSRGLALAAVLVTHHHRDHTGGNTWLRQRYNCAIYAPDNPR
IPAVSHVVRGGDSVAVPELGLALAVLATPGHTLDHVSYVGNGHLFCGDTLFGCGCGKLFEGDAAMMSASLDALAALPPTT
RVCCAHEYTLSNIDFAKTIDGANPALLERERIDRAARAQNRPTLPSTLALERTTNPFLRFHDADMRAFAVGELGSPDPGP
ATVFGAIRAAKDRWDG

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=269, Percent_Identity=36.8029739776952, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI21703352, Length=269, Percent_Identity=36.8029739776952, Blast_Score=157, Evalue=8e-39,
Organism=Homo sapiens, GI94538320, Length=259, Percent_Identity=38.2239382239382, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI94538322, Length=259, Percent_Identity=38.2239382239382, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI14150041, Length=259, Percent_Identity=35.5212355212355, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI46361987, Length=187, Percent_Identity=38.5026737967914, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786406, Length=255, Percent_Identity=41.5686274509804, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1787158, Length=158, Percent_Identity=29.746835443038, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=233, Percent_Identity=33.4763948497854, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320478, Length=272, Percent_Identity=28.3088235294118, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324614, Length=265, Percent_Identity=29.0566037735849, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356335, Length=258, Percent_Identity=37.5968992248062, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=37.3076923076923, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24667703, Length=258, Percent_Identity=37.5968992248062, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_THIDA (Q3SIB0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315423.1
- ProteinModelPortal:   Q3SIB0
- SMR:   Q3SIB0
- STRING:   Q3SIB0
- GeneID:   3673050
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1665
- NMPDR:   fig|292415.3.peg.1762
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   Q3SIB0
- BioCyc:   TDEN292415:TBD_1665-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27521; Mature: 27521

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLIPLPAFEDNYIWVWHDAKYAVAVDPGDPAVLSTYLDSRGLALAAVLVTHHHRDHTG
CEEEEECCCCCCCEEEEEECCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCC
GNTWLRQRYNCAIYAPDNPRIPAVSHVVRGGDSVAVPELGLALAVLATPGHTLDHVSYVG
CCHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCEECCCCCEEEEEEECCCCCHHHEEEEC
NGHLFCGDTLFGCGCGKLFEGDAAMMSASLDALAALPPTTRVCCAHEYTLSNIDFAKTID
CCEEEECCCEECCCCCCEECCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCCCHHHHCC
GANPALLERERIDRAARAQNRPTLPSTLALERTTNPFLRFHDADMRAFAVGELGSPDPGP
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCH
ATVFGAIRAAKDRWDG
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTLIPLPAFEDNYIWVWHDAKYAVAVDPGDPAVLSTYLDSRGLALAAVLVTHHHRDHTG
CEEEEECCCCCCCEEEEEECCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCC
GNTWLRQRYNCAIYAPDNPRIPAVSHVVRGGDSVAVPELGLALAVLATPGHTLDHVSYVG
CCHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCEECCCCCEEEEEEECCCCCHHHEEEEC
NGHLFCGDTLFGCGCGKLFEGDAAMMSASLDALAALPPTTRVCCAHEYTLSNIDFAKTID
CCEEEECCCEECCCCCCEECCCHHHHHHHHHHHHCCCCCCEEEEEECEECCCCCHHHHCC
GANPALLERERIDRAARAQNRPTLPSTLALERTTNPFLRFHDADMRAFAVGELGSPDPGP
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCH
ATVFGAIRAAKDRWDG
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA