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The map label for this gene is cheB

Identifier: 74317633

GI number: 74317633

Start: 1697024

End: 1698094

Strand: Reverse

Name: cheB

Synonym: Tbd_1615

Alternate gene names: 74317633

Gene position: 1698094-1697024 (Counterclockwise)

Preceding gene: 74317634

Following gene: 74317632

Centisome position: 58.36

GC content: 63.4

Gene sequence:

>1071_bases
ATGAAAATCAAGGTACTGATCGTCGACGACTCCGCGCTGATCCGCGGGGTGATGCGCGAAATTATCAACAGTCAGCCGGA
CATGGAGGTCGTCGGCGTCGCGCCCGACCCGATCGCCGCGCGCGAACTCATCAAGCAAACCAATCCCGATGTGCTTACGC
TCGACGTCGAGATGCCCAAGATGGATGGCCTCGAGTTCCTCGAAAAGCTCATGCGCCTGCGCCCGATGCCGGTCGTCATG
GTGTCGTCGCTGACCGAACGGGGTTCGGAAATCACGATGCGGGCGCTCGAACTTGGTGCGGTCGACTTCGTCACCAAGCC
CAAGATGTCGATCCAGAGCGGCATGCTCGAGTACACCGATCTCATTGCCGACAAGATCCGTATCGCCGCGCGCGCGCGCA
TCAAGCCGCGGACACCGCACGCGCAGGGCGGGCAAAGCGGAACGACGCTGGCCGCCGTGCGCAATCCGCTCACCAGCAGC
GAGAAGCTCATCATCATCGGCGCCTCGACCGGCGGCACCGAAGCGATCAAGGACTTCCTCATGCAGCTTCCGCCCGACAG
CCCGGGTGTTCTGATCACGCAGCACATGCCGGAAGGCTTTACCCGCTCCTTCGCCAACCGGCTCGACAAGTTGTGCAAGA
TCTCGGTCAAGGAAGCGGAGGGCGGCGAGCGTGTGCTGCCCGGGCATGCCTACCTCGCGCCCGGACACTCGCATCTGCTG
CTCGTCAGAAGCGGCGCCAACTACATGACCAAGCTCGACCAGGGTCCGCCGGTCAATCGCCACCGTCCCTCGGTCGACGT
ACTCTTCAACTCGGCCGCACTTACGGCCGGCAAGAACGCGGTCGGCGTGATCCTGACCGGCATGGGCAAGGATGGTGCCG
CCGGCATGCTCGAGATGAAAAAGGCGGGCGGTTACAACCTGGCGCAGGACGAGGCCTCGTGCGTGGTGTTCGGCATGCCC
AAGGAAGCCATTGCGGTTGGCGCGACGCACGAGGTTGCCCCTCTACATGAATTGCCGCGCCGCGTGCTCGAATTTTTCGC
AGCCCACGGCGAACGCGCGATGCGCGTATAA

Upstream 100 bases:

>100_bases
GGTGAAACGCGAACAGGATTACGCGAGCCGCCTCAAGAGCAACGTCGTCGCGGGCGAGGTCGATCTGTTCTGACCGACCG
CGGCTGTGCAGGAACGAAAA

Downstream 100 bases:

>100_bases
ACCGCGTCGCGGCGCGCTTTGCGGCACGGAGCGGCTTAAGTTTGCTATGCTCGTGCCGATTATTTATAAACACGATAAGC
CTCACTGACGGAGTACTGCA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKIKVLIVDDSALIRGVMREIINSQPDMEVVGVAPDPIAARELIKQTNPDVLTLDVEMPKMDGLEFLEKLMRLRPMPVVM
VSSLTERGSEITMRALELGAVDFVTKPKMSIQSGMLEYTDLIADKIRIAARARIKPRTPHAQGGQSGTTLAAVRNPLTSS
EKLIIIGASTGGTEAIKDFLMQLPPDSPGVLITQHMPEGFTRSFANRLDKLCKISVKEAEGGERVLPGHAYLAPGHSHLL
LVRSGANYMTKLDQGPPVNRHRPSVDVLFNSAALTAGKNAVGVILTGMGKDGAAGMLEMKKAGGYNLAQDEASCVVFGMP
KEAIAVGATHEVAPLHELPRRVLEFFAAHGERAMRV

Sequences:

>Translated_356_residues
MKIKVLIVDDSALIRGVMREIINSQPDMEVVGVAPDPIAARELIKQTNPDVLTLDVEMPKMDGLEFLEKLMRLRPMPVVM
VSSLTERGSEITMRALELGAVDFVTKPKMSIQSGMLEYTDLIADKIRIAARARIKPRTPHAQGGQSGTTLAAVRNPLTSS
EKLIIIGASTGGTEAIKDFLMQLPPDSPGVLITQHMPEGFTRSFANRLDKLCKISVKEAEGGERVLPGHAYLAPGHSHLL
LVRSGANYMTKLDQGPPVNRHRPSVDVLFNSAALTAGKNAVGVILTGMGKDGAAGMLEMKKAGGYNLAQDEASCVVFGMP
KEAIAVGATHEVAPLHELPRRVLEFFAAHGERAMRV
>Mature_356_residues
MKIKVLIVDDSALIRGVMREIINSQPDMEVVGVAPDPIAARELIKQTNPDVLTLDVEMPKMDGLEFLEKLMRLRPMPVVM
VSSLTERGSEITMRALELGAVDFVTKPKMSIQSGMLEYTDLIADKIRIAARARIKPRTPHAQGGQSGTTLAAVRNPLTSS
EKLIIIGASTGGTEAIKDFLMQLPPDSPGVLITQHMPEGFTRSFANRLDKLCKISVKEAEGGERVLPGHAYLAPGHSHLL
LVRSGANYMTKLDQGPPVNRHRPSVDVLFNSAALTAGKNAVGVILTGMGKDGAAGMLEMKKAGGYNLAQDEASCVVFGMP
KEAIAVGATHEVAPLHELPRRVLEFFAAHGERAMRV

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=347, Percent_Identity=66.8587896253602, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI87082052, Length=118, Percent_Identity=35.5932203389831, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_THIDA (Q3SIG0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315373.1
- ProteinModelPortal:   Q3SIG0
- SMR:   Q3SIG0
- STRING:   Q3SIG0
- GeneID:   3673000
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1615
- NMPDR:   fig|292415.3.peg.1615
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   FVTKPKL
- PhylomeDB:   Q3SIG0
- ProtClustDB:   PRK00742
- BioCyc:   TDEN292415:TBD_1615-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38395; Mature: 38395

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 169-169 ACT_SITE 195-195 ACT_SITE 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKVLIVDDSALIRGVMREIINSQPDMEVVGVAPDPIAARELIKQTNPDVLTLDVEMPK
CEEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
MDGLEFLEKLMRLRPMPVVMVSSLTERGSEITMRALELGAVDFVTKPKMSIQSGMLEYTD
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHH
LIADKIRIAARARIKPRTPHAQGGQSGTTLAAVRNPLTSSEKLIIIGASTGGTEAIKDFL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
MQLPPDSPGVLITQHMPEGFTRSFANRLDKLCKISVKEAEGGERVLPGHAYLAPGHSHLL
HHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCEECCCCEEECCCCCEEE
LVRSGANYMTKLDQGPPVNRHRPSVDVLFNSAALTAGKNAVGVILTGMGKDGAAGMLEMK
EEECCCHHHHHCCCCCCCCCCCCCCEEEEECHHHCCCCCEEEEEEEECCCCCCCCHHHHH
KAGGYNLAQDEASCVVFGMPKEAIAVGATHEVAPLHELPRRVLEFFAAHGERAMRV
HCCCCCCCCCCCCEEEEECCHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MKIKVLIVDDSALIRGVMREIINSQPDMEVVGVAPDPIAARELIKQTNPDVLTLDVEMPK
CEEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
MDGLEFLEKLMRLRPMPVVMVSSLTERGSEITMRALELGAVDFVTKPKMSIQSGMLEYTD
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHH
LIADKIRIAARARIKPRTPHAQGGQSGTTLAAVRNPLTSSEKLIIIGASTGGTEAIKDFL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
MQLPPDSPGVLITQHMPEGFTRSFANRLDKLCKISVKEAEGGERVLPGHAYLAPGHSHLL
HHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCEECCCCEEECCCCCEEE
LVRSGANYMTKLDQGPPVNRHRPSVDVLFNSAALTAGKNAVGVILTGMGKDGAAGMLEMK
EEECCCHHHHHCCCCCCCCCCCCCCEEEEECHHHCCCCCEEEEEEEECCCCCCCCHHHHH
KAGGYNLAQDEASCVVFGMPKEAIAVGATHEVAPLHELPRRVLEFFAAHGERAMRV
HCCCCCCCCCCCCEEEEECCHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA