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The map label for this gene is cfiB [H]

Identifier: 74317573

GI number: 74317573

Start: 1637955

End: 1639373

Strand: Direct

Name: cfiB [H]

Synonym: Tbd_1555

Alternate gene names: 74317573

Gene position: 1637955-1639373 (Clockwise)

Preceding gene: 74317557

Following gene: 74317574

Centisome position: 56.29

GC content: 63.07

Gene sequence:

>1419_bases
ATGATTAGGAAAATACTGGTCGCCAACCGCGGCGAGATCGCGGTGCGTATCGTCCGTGCCTGCGCCGAACTGGGAATCAA
GTCGGTCGCGATCTACACCGACGCCGACCGCCACGCACTCCACGTGAAGAAGGCCGACGAAGCCTATCACGTCGGCAAGG
ATCCAATTCTCGGCTATCTCAATGCCCATACGCTGGTGAACCTCGCCGCCGCGTCGGGCTGCGACGCGCTGCACCCTGGC
TACGGTTTCCTCTCCGAGAATCCCGATCTCGCGACGATTTGCGCGCGCCGCGGCATCCGTTTCATCGGCCCCTCGCCCGA
CGTCATCCGCCGCATGGGCGACAAGATCCAGGCGCGCAACGCGATGCTCGCGGCCGGCATCCCGGTCGTGCCCGGCTCCG
ACCACAATCTCGAAAGTGTCGACGAGGCGCGCGCGCTCGCGAACCAAATCGGCTACCCGGTGATGCTGAAGGCGACCAAT
GGCGGCGGCGGCCGTGGCATCCGCCGGTGCGACAGTGAGGACGAGCTACTGAAGAACTACGACCGCGTCGTGTCGGAAGC
GAGCAAGGCCTTCGGCAAGCCCGAAGTCTTCGTCGAGAAATGCGTCGTGCACCCCAAGCACATCGAGGTGCAGATCCTCG
GCGACACTCAGGGCAACATCATTCATCTGTTCGAGCGTGACTGCTCGATCCAGCGCCGCAACCAGAAGCTGATCGAGATC
GCGCCGAGCCCGCAGCTCACCGAGGCGCAGCGGCAGTACATCGGCAAGCTGGCGGTGCGCGCCGCAAAGGCGGTGGGCTA
CGTGAACGCAGGCACCGTCGAATTCCTGCTCGACCAGGACAACCAGTTCTACTTCATGGAAATGAACACGCGGCTGCAGG
TCGAGCACCCGATCACCGAGACGATCACCGGTGTCGACATCGTGCAGGAACAGATCCGCATCGCAGACGGGCAGCCGCTG
CAGTACAAGCAGGAAGACATCAAGCACCGCGGCTTCGCGATCGAATTCCGCGTCAACGCCGAAGACCCGAAGAACGAATT
CCTGCCGAGCCTCGGCCGCATCACGCGTTATTATTCGCCGGGCGGTCCGGGCGTGCGCGTCGACGCGGCGATCTACACCG
ATTACGTGATCCCTCCCTACTTCGACTCGATGTGCGCGAAGCTCACAGTGTGGAACCTCTCGTGGGAGTCGACGATCGAG
CGCGGCCGTCGTGCGCTCGCCGACATGCTCGTGTATGGCGTGAAGACGACGATTCCCTACTATCAGGAAATCCTCAAGAA
CCCCGAGTTCCGCAGCGGTCGTTTCAACACCGGCTTCGTCGAGACGCACCCCGAACTCACGCAGTACGTCGAAAAGAAAC
CGCCGGAAGTGATGGCCGCAGCCATCGCCGCCGCCATCGCCGCCCACCAGGGCTTGTAG

Upstream 100 bases:

>100_bases
AGGTCGACCACCTGATCCCGTCGCCTTACCCCGCGACCCGGTTAAACATTAGCCCGCAATGATATGATTTCGCGTTATTT
TTGTCAAAGAAACGGTGACC

Downstream 100 bases:

>100_bases
GAAGGCCCTCAAGACATGAACAAAGTATTCGTCACCGATCTCGTCCTGCGCGACGGCCACCAGTCGCTCATCGCCACCCG
CATGCGCACCGAGGACATGC

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: 2-oxoglutarate carboxylase beta subunit [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MIRKILVANRGEIAVRIVRACAELGIKSVAIYTDADRHALHVKKADEAYHVGKDPILGYLNAHTLVNLAAASGCDALHPG
YGFLSENPDLATICARRGIRFIGPSPDVIRRMGDKIQARNAMLAAGIPVVPGSDHNLESVDEARALANQIGYPVMLKATN
GGGGRGIRRCDSEDELLKNYDRVVSEASKAFGKPEVFVEKCVVHPKHIEVQILGDTQGNIIHLFERDCSIQRRNQKLIEI
APSPQLTEAQRQYIGKLAVRAAKAVGYVNAGTVEFLLDQDNQFYFMEMNTRLQVEHPITETITGVDIVQEQIRIADGQPL
QYKQEDIKHRGFAIEFRVNAEDPKNEFLPSLGRITRYYSPGGPGVRVDAAIYTDYVIPPYFDSMCAKLTVWNLSWESTIE
RGRRALADMLVYGVKTTIPYYQEILKNPEFRSGRFNTGFVETHPELTQYVEKKPPEVMAAAIAAAIAAHQGL

Sequences:

>Translated_472_residues
MIRKILVANRGEIAVRIVRACAELGIKSVAIYTDADRHALHVKKADEAYHVGKDPILGYLNAHTLVNLAAASGCDALHPG
YGFLSENPDLATICARRGIRFIGPSPDVIRRMGDKIQARNAMLAAGIPVVPGSDHNLESVDEARALANQIGYPVMLKATN
GGGGRGIRRCDSEDELLKNYDRVVSEASKAFGKPEVFVEKCVVHPKHIEVQILGDTQGNIIHLFERDCSIQRRNQKLIEI
APSPQLTEAQRQYIGKLAVRAAKAVGYVNAGTVEFLLDQDNQFYFMEMNTRLQVEHPITETITGVDIVQEQIRIADGQPL
QYKQEDIKHRGFAIEFRVNAEDPKNEFLPSLGRITRYYSPGGPGVRVDAAIYTDYVIPPYFDSMCAKLTVWNLSWESTIE
RGRRALADMLVYGVKTTIPYYQEILKNPEFRSGRFNTGFVETHPELTQYVEKKPPEVMAAAIAAAIAAHQGL
>Mature_472_residues
MIRKILVANRGEIAVRIVRACAELGIKSVAIYTDADRHALHVKKADEAYHVGKDPILGYLNAHTLVNLAAASGCDALHPG
YGFLSENPDLATICARRGIRFIGPSPDVIRRMGDKIQARNAMLAAGIPVVPGSDHNLESVDEARALANQIGYPVMLKATN
GGGGRGIRRCDSEDELLKNYDRVVSEASKAFGKPEVFVEKCVVHPKHIEVQILGDTQGNIIHLFERDCSIQRRNQKLIEI
APSPQLTEAQRQYIGKLAVRAAKAVGYVNAGTVEFLLDQDNQFYFMEMNTRLQVEHPITETITGVDIVQEQIRIADGQPL
QYKQEDIKHRGFAIEFRVNAEDPKNEFLPSLGRITRYYSPGGPGVRVDAAIYTDYVIPPYFDSMCAKLTVWNLSWESTIE
RGRRALADMLVYGVKTTIPYYQEILKNPEFRSGRFNTGFVETHPELTQYVEKKPPEVMAAAIAAAIAAHQGL

Specific function: This Protein Is A Component Of The Acetyl Coenzyme A Carboxylase Complex; First, Biotin Carboxylase Catalyzes The Carboxylation Of The Carrier Protein And Then The Transcarboxylase Transfers The Carboxyl Group To Form Malonyl-CoA. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=453, Percent_Identity=49.2273730684327, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI106049295, Length=453, Percent_Identity=49.2273730684327, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI106049292, Length=453, Percent_Identity=49.2273730684327, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI116805327, Length=444, Percent_Identity=44.8198198198198, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI189095269, Length=446, Percent_Identity=45.9641255605381, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI65506442, Length=446, Percent_Identity=45.9641255605381, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI295821183, Length=446, Percent_Identity=45.9641255605381, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI38679977, Length=528, Percent_Identity=32.0075757575758, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI38679967, Length=528, Percent_Identity=32.0075757575758, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI38679960, Length=528, Percent_Identity=32.0075757575758, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI38679974, Length=528, Percent_Identity=32.0075757575758, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI38679971, Length=528, Percent_Identity=32.0075757575758, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI134142062, Length=529, Percent_Identity=31.0018903591682, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI18105007, Length=237, Percent_Identity=25.7383966244726, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI170295797, Length=233, Percent_Identity=24.8927038626609, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI21361331, Length=233, Percent_Identity=24.8927038626609, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI169790915, Length=233, Percent_Identity=24.8927038626609, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=49.4356659142212, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17562816, Length=452, Percent_Identity=50.2212389380531, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71987519, Length=474, Percent_Identity=45.1476793248945, Blast_Score=381, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17567343, Length=472, Percent_Identity=44.2796610169491, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71997163, Length=463, Percent_Identity=30.4535637149028, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71997168, Length=463, Percent_Identity=30.4535637149028, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI133931226, Length=399, Percent_Identity=35.0877192982456, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6319695, Length=458, Percent_Identity=45.6331877729258, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321376, Length=458, Percent_Identity=46.5065502183406, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=40, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6323863, Length=496, Percent_Identity=33.0645161290323, Blast_Score=232, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6324343, Length=541, Percent_Identity=30.8687615526802, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI281363050, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652224, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652222, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652220, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652218, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652212, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652210, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652214, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI19921944, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24652216, Length=454, Percent_Identity=48.2378854625551, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651757, Length=467, Percent_Identity=43.8972162740899, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24651759, Length=429, Percent_Identity=43.5897435897436, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI161076409, Length=523, Percent_Identity=30.5927342256214, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24586458, Length=523, Percent_Identity=30.5927342256214, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI161076407, Length=523, Percent_Identity=30.5927342256214, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24586460, Length=523, Percent_Identity=30.5927342256214, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.7 [H]

Molecular weight: Translated: 52410; Mature: 52410

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKILVANRGEIAVRIVRACAELGIKSVAIYTDADRHALHVKKADEAYHVGKDPILGYL
CCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCHHCCCCCCHHHHH
NAHTLVNLAAASGCDALHPGYGFLSENPDLATICARRGIRFIGPSPDVIRRMGDKIQARN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHCC
AMLAAGIPVVPGSDHNLESVDEARALANQIGYPVMLKATNGGGGRGIRRCDSEDELLKNY
CEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
DRVVSEASKAFGKPEVFVEKCVVHPKHIEVQILGDTQGNIIHLFERDCSIQRRNQKLIEI
HHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCHHHCCCEEEEE
APSPQLTEAQRQYIGKLAVRAAKAVGYVNAGTVEFLLDQDNQFYFMEMNTRLQVEHPITE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCEEEECCCHHH
TITGVDIVQEQIRIADGQPLQYKQEDIKHRGFAIEFRVNAEDPKNEFLPSLGRITRYYSP
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHEEECC
GGPGVRVDAAIYTDYVIPPYFDSMCAKLTVWNLSWESTIERGRRALADMLVYGVKTTIPY
CCCCEEEEEEEEECCCCCCHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHH
YQEILKNPEFRSGRFNTGFVETHPELTQYVEKKPPEVMAAAIAAAIAAHQGL
HHHHHCCCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRKILVANRGEIAVRIVRACAELGIKSVAIYTDADRHALHVKKADEAYHVGKDPILGYL
CCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCHHCCCCCCHHHHH
NAHTLVNLAAASGCDALHPGYGFLSENPDLATICARRGIRFIGPSPDVIRRMGDKIQARN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHCC
AMLAAGIPVVPGSDHNLESVDEARALANQIGYPVMLKATNGGGGRGIRRCDSEDELLKNY
CEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
DRVVSEASKAFGKPEVFVEKCVVHPKHIEVQILGDTQGNIIHLFERDCSIQRRNQKLIEI
HHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCHHHCCCEEEEE
APSPQLTEAQRQYIGKLAVRAAKAVGYVNAGTVEFLLDQDNQFYFMEMNTRLQVEHPITE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCEEEECCCHHH
TITGVDIVQEQIRIADGQPLQYKQEDIKHRGFAIEFRVNAEDPKNEFLPSLGRITRYYSP
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHEEECC
GGPGVRVDAAIYTDYVIPPYFDSMCAKLTVWNLSWESTIERGRRALADMLVYGVKTTIPY
CCCCEEEEEEEEECCCCCCHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHH
YQEILKNPEFRSGRFNTGFVETHPELTQYVEKKPPEVMAAAIAAAIAAHQGL
HHHHHCCCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA