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The map label for this gene is pycB [H]

Identifier: 74317574

GI number: 74317574

Start: 1639389

End: 1641236

Strand: Direct

Name: pycB [H]

Synonym: Tbd_1556

Alternate gene names: 74317574

Gene position: 1639389-1641236 (Clockwise)

Preceding gene: 74317573

Following gene: 74317575

Centisome position: 56.34

GC content: 66.34

Gene sequence:

>1848_bases
ATGAACAAAGTATTCGTCACCGATCTCGTCCTGCGCGACGGCCACCAGTCGCTCATCGCCACCCGCATGCGCACCGAGGA
CATGCTGCCGATCTGCGGCAAGCTCGACCAGGTCGGCTTCTGGTCGCTCGAAGCCTGGGGTGGAGCAACCTTCGACGCCT
GCGTGCGCTTCCTGCGCGAAGACCCGTGGGAGCGCCTGCGCAAGCTGCGTAAAGCGCTGCCCAACACACCGATCCAGATG
CTACTGCGCGGGCAGAACCTGCTCGGCTACCGCAACTATTCGGACGACGTCGTGCGCGCGTTCGTTCAGAAATCTGCCGA
CAACGGGGTCGACGTGTTCCGCGTGTTCGACGCGATGAACGACGTACGCAACCTGCGCGTCTCGATCGAAGCCGTCAAAG
CGGCCGGCAAGCACGCCGAAGGCGCGATCAGCTACACCACGAGCCCGGTGCACGACATCCCGCACTTCGTCACCCTCGCG
CGCGGCCTCGCCGAGATGGGCTGCGACACGATCGCGATCAAGGACATGGCGGGCTTGCTCACGCCTTACACCGCGTACGA
CCTCGTGTGCGCGATCCGCGAAGCGACGCACCTGCCGATCCACACGCACAGCCACGCGACGTCGGGCCTCGCACACATGA
CGCACCTCAAGGCGGTCGAGGCCGGCGCGACCATCATCGACACCAGCGTCGGGGCGTTTGCCGAAGGCGCGAGTCACCCG
ACGACGCAAAGCCTCGTCGCCGCGCTCGCCGGCACCGCTTACGACACCGGACTGTCGTTGCCGCTGATCGAGGAGATCTC
GGCCTACTTTAAGGACGTGCGCAAGAAATACTGGCAGTTCGAATCCGCCTTCACCGGCACCGACACCCGCGTCCTGATCA
ACCAGGTGCCGGGGGGAATGATCTCCAACCTCGCCAACCAGCTCAAGGAACAGGGCGCGCTCGACAAGATGGACGCCGTG
CTCGAGGAGATTCCGCGGGTACGCGAGGACCTTGGCTACCCGCCGCTGGTGACGCCTACCTCGCAGATCGTCGGCACGCA
GGCGGCGCTCAACGTCCTCACCGGCGCACGCTACAAGTCGGTCACGAACGAGGTGAAGCTCTATCTTCAGGGGCGCTACG
GACGCGCTCCCGGGCATGTCAACGAGGAAGTGCGCAAAATGGCCATCGGCGACCTCGACGTCACGACCGGCCGTCCGGCC
GACCTGCTCGGCGACGAGCTCGACAAGCTGCGCGGCGAAATCGAGGGCCTGGCCAAGTCGGACGAGGACGTGCTGACCTA
CGCGATGTTCCCCGAGATCGGCAAGACCTGGCTGCAGGAGCGCGCGGCCGGAAGCCTGAAGCCCGAGCCGCTGCTGCCGC
CGGGCGCGACCGAACAGGCGAGCACGACCCGCTACGCCGCCGACGAATTCAAGGTCACGCTGCACGGCGAGACCTACCAC
ATCAAGCTCTCGGGAAGCGGTCGCAGCGAGGCCGCGCAGCGGCCCTACTTCGTATCGATCGACGGGGTCTCCGAGGAAGT
TCTGGTCGAGCCCTTGAACGAGGTCCCCCTGTCGGGCGGCGCCGACACGCCGCGCAAATTGCCGGCCGAAGCGGCGTCCG
CGCCGGGGGAAAAACCGCGCGCGCGCCATCCCGGTCACGTCACGGCGGCGATGCCGGGCAACGTCGTCGACGTTCTCGTC
AGCGTCGGCCAGACCGTCCAGGCCGGCGACGGCGTGCTCGTGATCGAAGCGATGAAGATGGAAAGCGAAGTCCAGGCGCC
GATCGCCGGTGTCGTCATCAACCTGTTCGCCAAGAAAGGCGACGCGGTGACGCCCGACATGGCGCTCGTCGAGATCCAGC
CCGCATGA

Upstream 100 bases:

>100_bases
ACTCACGCAGTACGTCGAAAAGAAACCGCCGGAAGTGATGGCCGCAGCCATCGCCGCCGCCATCGCCGCCCACCAGGGCT
TGTAGGAAGGCCCTCAAGAC

Downstream 100 bases:

>100_bases
CGCGCACGAGCCCCACGCTCGTCCTCGCCTCGACCTCGCGCTACCGGCGCGAACTGTTGGCGCGGCTCCGCCTGCCCTTC
GAGGTGCTCGCGCCGAACGT

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MNKVFVTDLVLRDGHQSLIATRMRTEDMLPICGKLDQVGFWSLEAWGGATFDACVRFLREDPWERLRKLRKALPNTPIQM
LLRGQNLLGYRNYSDDVVRAFVQKSADNGVDVFRVFDAMNDVRNLRVSIEAVKAAGKHAEGAISYTTSPVHDIPHFVTLA
RGLAEMGCDTIAIKDMAGLLTPYTAYDLVCAIREATHLPIHTHSHATSGLAHMTHLKAVEAGATIIDTSVGAFAEGASHP
TTQSLVAALAGTAYDTGLSLPLIEEISAYFKDVRKKYWQFESAFTGTDTRVLINQVPGGMISNLANQLKEQGALDKMDAV
LEEIPRVREDLGYPPLVTPTSQIVGTQAALNVLTGARYKSVTNEVKLYLQGRYGRAPGHVNEEVRKMAIGDLDVTTGRPA
DLLGDELDKLRGEIEGLAKSDEDVLTYAMFPEIGKTWLQERAAGSLKPEPLLPPGATEQASTTRYAADEFKVTLHGETYH
IKLSGSGRSEAAQRPYFVSIDGVSEEVLVEPLNEVPLSGGADTPRKLPAEAASAPGEKPRARHPGHVTAAMPGNVVDVLV
SVGQTVQAGDGVLVIEAMKMESEVQAPIAGVVINLFAKKGDAVTPDMALVEIQPA

Sequences:

>Translated_615_residues
MNKVFVTDLVLRDGHQSLIATRMRTEDMLPICGKLDQVGFWSLEAWGGATFDACVRFLREDPWERLRKLRKALPNTPIQM
LLRGQNLLGYRNYSDDVVRAFVQKSADNGVDVFRVFDAMNDVRNLRVSIEAVKAAGKHAEGAISYTTSPVHDIPHFVTLA
RGLAEMGCDTIAIKDMAGLLTPYTAYDLVCAIREATHLPIHTHSHATSGLAHMTHLKAVEAGATIIDTSVGAFAEGASHP
TTQSLVAALAGTAYDTGLSLPLIEEISAYFKDVRKKYWQFESAFTGTDTRVLINQVPGGMISNLANQLKEQGALDKMDAV
LEEIPRVREDLGYPPLVTPTSQIVGTQAALNVLTGARYKSVTNEVKLYLQGRYGRAPGHVNEEVRKMAIGDLDVTTGRPA
DLLGDELDKLRGEIEGLAKSDEDVLTYAMFPEIGKTWLQERAAGSLKPEPLLPPGATEQASTTRYAADEFKVTLHGETYH
IKLSGSGRSEAAQRPYFVSIDGVSEEVLVEPLNEVPLSGGADTPRKLPAEAASAPGEKPRARHPGHVTAAMPGNVVDVLV
SVGQTVQAGDGVLVIEAMKMESEVQAPIAGVVINLFAKKGDAVTPDMALVEIQPA
>Mature_615_residues
MNKVFVTDLVLRDGHQSLIATRMRTEDMLPICGKLDQVGFWSLEAWGGATFDACVRFLREDPWERLRKLRKALPNTPIQM
LLRGQNLLGYRNYSDDVVRAFVQKSADNGVDVFRVFDAMNDVRNLRVSIEAVKAAGKHAEGAISYTTSPVHDIPHFVTLA
RGLAEMGCDTIAIKDMAGLLTPYTAYDLVCAIREATHLPIHTHSHATSGLAHMTHLKAVEAGATIIDTSVGAFAEGASHP
TTQSLVAALAGTAYDTGLSLPLIEEISAYFKDVRKKYWQFESAFTGTDTRVLINQVPGGMISNLANQLKEQGALDKMDAV
LEEIPRVREDLGYPPLVTPTSQIVGTQAALNVLTGARYKSVTNEVKLYLQGRYGRAPGHVNEEVRKMAIGDLDVTTGRPA
DLLGDELDKLRGEIEGLAKSDEDVLTYAMFPEIGKTWLQERAAGSLKPEPLLPPGATEQASTTRYAADEFKVTLHGETYH
IKLSGSGRSEAAQRPYFVSIDGVSEEVLVEPLNEVPLSGGADTPRKLPAEAASAPGEKPRARHPGHVTAAMPGNVVDVLV
SVGQTVQAGDGVLVIEAMKMESEVQAPIAGVVINLFAKKGDAVTPDMALVEIQPA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=638, Percent_Identity=34.1692789968652, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI106049295, Length=638, Percent_Identity=34.1692789968652, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI106049292, Length=638, Percent_Identity=34.1692789968652, Blast_Score=302, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17562816, Length=637, Percent_Identity=35.0078492935636, Blast_Score=331, Evalue=6e-91,
Organism=Saccharomyces cerevisiae, GI6319695, Length=643, Percent_Identity=32.6594090202177, Blast_Score=304, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6321376, Length=641, Percent_Identity=32.1372854914197, Blast_Score=300, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24652212, Length=625, Percent_Identity=35.68, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24652210, Length=625, Percent_Identity=35.68, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24652214, Length=625, Percent_Identity=35.68, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI19921944, Length=625, Percent_Identity=35.68, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24652216, Length=625, Percent_Identity=35.68, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI281363050, Length=625, Percent_Identity=35.68, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24652224, Length=625, Percent_Identity=35.68, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24652222, Length=625, Percent_Identity=35.68, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24652220, Length=625, Percent_Identity=35.68, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24652218, Length=625, Percent_Identity=35.68, Blast_Score=322, Evalue=4e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 66478; Mature: 66478

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVFVTDLVLRDGHQSLIATRMRTEDMLPICGKLDQVGFWSLEAWGGATFDACVRFLRE
CCCEEEEHHHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHC
DPWERLRKLRKALPNTPIQMLLRGQNLLGYRNYSDDVVRAFVQKSADNGVDVFRVFDAMN
CHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
DVRNLRVSIEAVKAAGKHAEGAISYTTSPVHDIPHFVTLARGLAEMGCDTIAIKDMAGLL
HHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
TPYTAYDLVCAIREATHLPIHTHSHATSGLAHMTHLKAVEAGATIIDTSVGAFAEGASHP
CCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCC
TTQSLVAALAGTAYDTGLSLPLIEEISAYFKDVRKKYWQFESAFTGTDTRVLINQVPGGM
HHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH
ISNLANQLKEQGALDKMDAVLEEIPRVREDLGYPPLVTPTSQIVGTQAALNVLTGARYKS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHH
VTNEVKLYLQGRYGRAPGHVNEEVRKMAIGDLDVTTGRPADLLGDELDKLRGEIEGLAKS
HHHHEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DEDVLTYAMFPEIGKTWLQERAAGSLKPEPLLPPGATEQASTTRYAADEFKVTLHGETYH
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEECCEEEEEEECCEEE
IKLSGSGRSEAAQRPYFVSIDGVSEEVLVEPLNEVPLSGGADTPRKLPAEAASAPGEKPR
EEECCCCCCHHHCCCEEEEECCCCHHHHHCCHHHCCCCCCCCCCCCCCCHHHCCCCCCCC
ARHPGHVTAAMPGNVVDVLVSVGQTVQAGDGVLVIEAMKMESEVQAPIAGVVINLFAKKG
CCCCCEEEEECCCHHHHHHHHCCCCEECCCCEEEEEEHHHHHHHHCCHHHHHHHHHHCCC
DAVTPDMALVEIQPA
CCCCCCEEEEEEECC
>Mature Secondary Structure
MNKVFVTDLVLRDGHQSLIATRMRTEDMLPICGKLDQVGFWSLEAWGGATFDACVRFLRE
CCCEEEEHHHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHC
DPWERLRKLRKALPNTPIQMLLRGQNLLGYRNYSDDVVRAFVQKSADNGVDVFRVFDAMN
CHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
DVRNLRVSIEAVKAAGKHAEGAISYTTSPVHDIPHFVTLARGLAEMGCDTIAIKDMAGLL
HHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
TPYTAYDLVCAIREATHLPIHTHSHATSGLAHMTHLKAVEAGATIIDTSVGAFAEGASHP
CCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCC
TTQSLVAALAGTAYDTGLSLPLIEEISAYFKDVRKKYWQFESAFTGTDTRVLINQVPGGM
HHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH
ISNLANQLKEQGALDKMDAVLEEIPRVREDLGYPPLVTPTSQIVGTQAALNVLTGARYKS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHH
VTNEVKLYLQGRYGRAPGHVNEEVRKMAIGDLDVTTGRPADLLGDELDKLRGEIEGLAKS
HHHHEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DEDVLTYAMFPEIGKTWLQERAAGSLKPEPLLPPGATEQASTTRYAADEFKVTLHGETYH
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEECCEEEEEEECCEEE
IKLSGSGRSEAAQRPYFVSIDGVSEEVLVEPLNEVPLSGGADTPRKLPAEAASAPGEKPR
EEECCCCCCHHHCCCEEEEECCCCHHHHHCCHHHCCCCCCCCCCCCCCCHHHCCCCCCCC
ARHPGHVTAAMPGNVVDVLVSVGQTVQAGDGVLVIEAMKMESEVQAPIAGVVINLFAKKG
CCCCCEEEEECCCHHHHHHHHCCCCEECCCCEEEEEEHHHHHHHHCCHHHHHHHHHHCCC
DAVTPDMALVEIQPA
CCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]