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The map label for this gene is holB [H]

Identifier: 74317560

GI number: 74317560

Start: 1626767

End: 1627750

Strand: Reverse

Name: holB [H]

Synonym: Tbd_1542

Alternate gene names: 74317560

Gene position: 1627750-1626767 (Counterclockwise)

Preceding gene: 74317561

Following gene: 74317559

Centisome position: 55.94

GC content: 68.19

Gene sequence:

>984_bases
GTGAGCGTGGCCTATCCCTGGCTCGAACCGGTCTGGCAGCGCCTGCTCGCGACGCGGTCCCGCCCTGCGCAAGCGCTGCT
TCTCGCCGGTCCGCGCGGCGTCGGCAAAAGCGCGCTTGCGCTCGAGTGGGCGCGCGCCTTGCTCTGCGAGGCGCCTAGAC
CCGACGGCTCGGCGTGCGGCGTCTGCCCGGCCTGCCACTGGTTCGAGACCGGCGCCCACCCCGACTTCCGGCGGGTGGGT
CTGCAGGAAAAGACGAGCAAGGAGGGCGAAACGCGTCTGGCGACCGCGATCGAGGTCGACCAGGCGCGCGACGCGGTCGA
CTTCGTCCAGCTTTCCACCTATCGCGCCGGCTATCGCGTGGTTCTGGTCGATCCCGCCGACAGTCTCAATCTGGCAGCGG
CGAACGCGCTGTTAAAGGTGCTCGAGGAACCGCCGTTAAATACGGTGTTCGTGCTGGTGGCGGATCAACCGCGGCGCCTG
CTGCCGACCATACGCAGTCGGTGTACGCGGCTCGATGTCGGACTGCCGGCTTTGGCGCAGGCGACGGCGTGGCTCGGCGA
GCAGGGCGTGGACGACGCGCCGACGCTGCTGGCCTTGGCGGGCGGCACGCCGCTCGCCGCGCTGGGATGGTGCGAAGGCG
AGGTGCTCGACGAGCGCCGCGGGGTGCTCGAAGGACTTGCGCGTCCCCAAGCGCTCGACCCGGTCACCCTCGGCGAGCGC
TGGAAGGCCGTTGCGCCGCAGACCTGGCACGATGTCGCGTACAAATGGCTAGGTGACCTACTGGCTGTGCGTCTGGGGGG
CAGCGTGCAGTTCAACCAGGATTTTATCGACGTACTGGCAACGCTCGGCCGTGAAGCCGACCTGGGAAAGCTCCTCGCGC
TGGCAAAGACACAGGCCGACGAGGGCAGGACGCTGGCCCACCCGCTCAACCGCAGTCTGCAATTGCAGGCCTGGCTGGTC
GAATACCGGCATGTCTTTGATTAA

Upstream 100 bases:

>100_bases
TGCGTGCGCGTGCGGAGCCGCATCGCATCCACGTGCTCGACGCGCGGCAGACGATCGACGCCGTGCAGGCCGAAATCGCG
CGGCTGCTGCAGGACTTGAC

Downstream 100 bases:

>100_bases
GAGAGTCGATCATGAACGCAGCAGTCAACGAGGTTACAGCGCCGGTCCGCCCCGGTGTGCTGTCGCTGTCGATCAAGGAG
AAGTCCGCGCTGTTTGCCGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSVAYPWLEPVWQRLLATRSRPAQALLLAGPRGVGKSALALEWARALLCEAPRPDGSACGVCPACHWFETGAHPDFRRVG
LQEKTSKEGETRLATAIEVDQARDAVDFVQLSTYRAGYRVVLVDPADSLNLAAANALLKVLEEPPLNTVFVLVADQPRRL
LPTIRSRCTRLDVGLPALAQATAWLGEQGVDDAPTLLALAGGTPLAALGWCEGEVLDERRGVLEGLARPQALDPVTLGER
WKAVAPQTWHDVAYKWLGDLLAVRLGGSVQFNQDFIDVLATLGREADLGKLLALAKTQADEGRTLAHPLNRSLQLQAWLV
EYRHVFD

Sequences:

>Translated_327_residues
MSVAYPWLEPVWQRLLATRSRPAQALLLAGPRGVGKSALALEWARALLCEAPRPDGSACGVCPACHWFETGAHPDFRRVG
LQEKTSKEGETRLATAIEVDQARDAVDFVQLSTYRAGYRVVLVDPADSLNLAAANALLKVLEEPPLNTVFVLVADQPRRL
LPTIRSRCTRLDVGLPALAQATAWLGEQGVDDAPTLLALAGGTPLAALGWCEGEVLDERRGVLEGLARPQALDPVTLGER
WKAVAPQTWHDVAYKWLGDLLAVRLGGSVQFNQDFIDVLATLGREADLGKLLALAKTQADEGRTLAHPLNRSLQLQAWLV
EYRHVFD
>Mature_326_residues
SVAYPWLEPVWQRLLATRSRPAQALLLAGPRGVGKSALALEWARALLCEAPRPDGSACGVCPACHWFETGAHPDFRRVGL
QEKTSKEGETRLATAIEVDQARDAVDFVQLSTYRAGYRVVLVDPADSLNLAAANALLKVLEEPPLNTVFVLVADQPRRLL
PTIRSRCTRLDVGLPALAQATAWLGEQGVDDAPTLLALAGGTPLAALGWCEGEVLDERRGVLEGLARPQALDPVTLGERW
KAVAPQTWHDVAYKWLGDLLAVRLGGSVQFNQDFIDVLATLGREADLGKLLALAKTQADEGRTLAHPLNRSLQLQAWLVE
YRHVFD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=228, Percent_Identity=35.0877192982456, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786676, Length=185, Percent_Identity=34.0540540540541, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35624; Mature: 35493

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAYPWLEPVWQRLLATRSRPAQALLLAGPRGVGKSALALEWARALLCEAPRPDGSACG
CCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
VCPACHWFETGAHPDFRRVGLQEKTSKEGETRLATAIEVDQARDAVDFVQLSTYRAGYRV
CCCCHHHHCCCCCCHHHHCCCCHHCCCCCCHHHHHHEEHHHHHHHHHHHHHHHCCCCCEE
VLVDPADSLNLAAANALLKVLEEPPLNTVFVLVADQPRRLLPTIRSRCTRLDVGLPALAQ
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHH
ATAWLGEQGVDDAPTLLALAGGTPLAALGWCEGEVLDERRGVLEGLARPQALDPVTLGER
HHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
WKAVAPQTWHDVAYKWLGDLLAVRLGGSVQFNQDFIDVLATLGREADLGKLLALAKTQAD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EGRTLAHPLNRSLQLQAWLVEYRHVFD
CCCCHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVAYPWLEPVWQRLLATRSRPAQALLLAGPRGVGKSALALEWARALLCEAPRPDGSACG
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
VCPACHWFETGAHPDFRRVGLQEKTSKEGETRLATAIEVDQARDAVDFVQLSTYRAGYRV
CCCCHHHHCCCCCCHHHHCCCCHHCCCCCCHHHHHHEEHHHHHHHHHHHHHHHCCCCCEE
VLVDPADSLNLAAANALLKVLEEPPLNTVFVLVADQPRRLLPTIRSRCTRLDVGLPALAQ
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHH
ATAWLGEQGVDDAPTLLALAGGTPLAALGWCEGEVLDERRGVLEGLARPQALDPVTLGER
HHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
WKAVAPQTWHDVAYKWLGDLLAVRLGGSVQFNQDFIDVLATLGREADLGKLLALAKTQAD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EGRTLAHPLNRSLQLQAWLVEYRHVFD
CCCCHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]