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The map label for this gene is yfkN [H]

Identifier: 74317310

GI number: 74317310

Start: 1363389

End: 1365176

Strand: Direct

Name: yfkN [H]

Synonym: Tbd_1292

Alternate gene names: 74317310

Gene position: 1363389-1365176 (Clockwise)

Preceding gene: 74317309

Following gene: 74317311

Centisome position: 46.85

GC content: 64.77

Gene sequence:

>1788_bases
ATGCAAAAAGGGATTCTTTCCCGGGCCGTACTGGCCACCTTCGCCGCCGGCGCGATCACGGCGGCATTTCCTGTGGCGGG
CCAGACGCTCGCCACGACCGCGGCCACCCTGCCGGTGCAGAAGCCCGCCCCGGACAGCAAGACCATGAAACTCACGCTGC
TGCAGATGGGCGACATTCATGGCCACATGATCCCGCGCGCCCACCTGCGCAACGACGGCAACGGCGCGATGCTCGGTGGC
CTCGCCTACATGTACAGCAAGATCCAGGAGATTCGCGCCAGGAATCCGAACACGCTGTTGTTCAACACCGGTGACACCAT
CCAGGGCAGCGCCGAGGCGCTCTACACCAAGGGTCAGGCGCTCGTCGACGTGCTCGACCGCTTCGGCGTCGTCGGCTTCG
CGCCCGGCAACTGGGATTACCTCTACGGCGCCGAGCGCTTCGTCGAGCTCTTCGGCAGCGGACGCTGGGGCGCGGTCGCG
GCGAACGTCTATTACGATCCCGCGATCTACCCCGACAAGGCTGGCCAGACTGTCCTGCCGCCTTACCGCATCCTGACCGT
GAACGGCCTGAAGATCGGCGTCATGGGCCTGAGCACCGAGCGCATCACGCTGGCCCCGGGACCGTTCGCCACCGCCGGCC
TGATCGCGACCAGCGAGGGCCAGGAAGTCCCCGGCGTCATCGACACCCTGCGCAATCAGGAAAAGGTCGACCTCGTCATT
CTGGTTTCTGAATTCGGCCTGGCGAAGAACGTCCATTTCGCCGAAAACTTCCCCGGCATCGACGTCGTCCTGTCGGCCGA
CATGCACGAGGAAACCCGCGATCCGATCACGACCAGCAACGGCACGATCATCAGCGAGGTCGGCCAGGACGGTACGCGTC
TCGGTCAGATCGATCTCGACATCCGTGACGGCAAGATCGCGAGCTGGAAATACACCTTCAACACGATCGACACGAGCACG
ATCCGTCCGAACCCGGTCATCGCGCAACTGGTCGCCGAGAAGCGCAAGGAGTTCGTCGCCGGCAAGTTCTTCAAGAAGCA
CGTCAATCCGTTCAACGGCATGGTGCTGAAGACGCCGATCGACAAGGTCGTCGGCAAGGCCGATGTCGCGCTGCATCGTT
CCAACTACTCGGACGCCGCCGTTCCGGGCGCGATCGAGGGAACGTCGCACAACTTCATCGCCGATGCCTTCCGCGAGCAG
GCCGGTGCCGATGTCGGCCACATGCGGGGCTTCCGTTACGGCACGCACATCGCGCCGGGCGACATCAAGCTCGACGATCT
GTACCACTACATCGCGATCGGGCCCCAAGTGGCGAAGACGACGATCACCGGCCAGATGCTCAAGGACGATCTTGAAAACA
ACGCCGCCGGCTCGTTCAACTCGGACATCTTCAAGTGGACGGGCGGCTGGCAGCAGGCGCTGAGCGGCGTGCGCTACGAC
CTCGACCTCTACGCCTCCCTGGGCGCACGCGTGCAGAACGCGCAGGTGTTCAACCAGGCGACCGGTCAGTGGGAGCCGCT
GGATCCGGCCAAGAGCTACAGCTACGCGGGCTATTGGTACGACCAGGCGCCGAAGAAGGTCGGGGCGCTGGTTTCCGCGA
ACCCCGTCACTGCGGTGAAGGGCGCCGCCGGCGAGACGCTGGACGGCACCGAGGTCGTCGTCAACTACCTCAAGACGCAC
AGCGCGAACCCGGGTGAGAGCCGTATCCGCCTGGTGAACCCGCTGCCGGCGGCGGCTTACGGCAGCGCCGAGATCCAGCC
GCTGAAAGGCGTGAGCAGCGCGTACTGA

Upstream 100 bases:

>100_bases
GGACGGCACGCTGTTCAGCGACGCACCGCGTTCGACCTACTAAAGCCAACCCGAGGGGGCTCGCCCCCCTCGTATTCAAC
CTCAAGTGGAGCAACGAAAA

Downstream 100 bases:

>100_bases
GGCGTGCGGGGGGCGTCGCCCGAGTGGGCGGCGCGGCAGTCCCCCGTCGCAGTAAATGTTGCGGGCGTCGATCGACGTCG
ACCGGCGCCCGCAGCCAACG

Product: putative 5'-nucleotidase/2' 3'-cyclic phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MQKGILSRAVLATFAAGAITAAFPVAGQTLATTAATLPVQKPAPDSKTMKLTLLQMGDIHGHMIPRAHLRNDGNGAMLGG
LAYMYSKIQEIRARNPNTLLFNTGDTIQGSAEALYTKGQALVDVLDRFGVVGFAPGNWDYLYGAERFVELFGSGRWGAVA
ANVYYDPAIYPDKAGQTVLPPYRILTVNGLKIGVMGLSTERITLAPGPFATAGLIATSEGQEVPGVIDTLRNQEKVDLVI
LVSEFGLAKNVHFAENFPGIDVVLSADMHEETRDPITTSNGTIISEVGQDGTRLGQIDLDIRDGKIASWKYTFNTIDTST
IRPNPVIAQLVAEKRKEFVAGKFFKKHVNPFNGMVLKTPIDKVVGKADVALHRSNYSDAAVPGAIEGTSHNFIADAFREQ
AGADVGHMRGFRYGTHIAPGDIKLDDLYHYIAIGPQVAKTTITGQMLKDDLENNAAGSFNSDIFKWTGGWQQALSGVRYD
LDLYASLGARVQNAQVFNQATGQWEPLDPAKSYSYAGYWYDQAPKKVGALVSANPVTAVKGAAGETLDGTEVVVNYLKTH
SANPGESRIRLVNPLPAAAYGSAEIQPLKGVSSAY

Sequences:

>Translated_595_residues
MQKGILSRAVLATFAAGAITAAFPVAGQTLATTAATLPVQKPAPDSKTMKLTLLQMGDIHGHMIPRAHLRNDGNGAMLGG
LAYMYSKIQEIRARNPNTLLFNTGDTIQGSAEALYTKGQALVDVLDRFGVVGFAPGNWDYLYGAERFVELFGSGRWGAVA
ANVYYDPAIYPDKAGQTVLPPYRILTVNGLKIGVMGLSTERITLAPGPFATAGLIATSEGQEVPGVIDTLRNQEKVDLVI
LVSEFGLAKNVHFAENFPGIDVVLSADMHEETRDPITTSNGTIISEVGQDGTRLGQIDLDIRDGKIASWKYTFNTIDTST
IRPNPVIAQLVAEKRKEFVAGKFFKKHVNPFNGMVLKTPIDKVVGKADVALHRSNYSDAAVPGAIEGTSHNFIADAFREQ
AGADVGHMRGFRYGTHIAPGDIKLDDLYHYIAIGPQVAKTTITGQMLKDDLENNAAGSFNSDIFKWTGGWQQALSGVRYD
LDLYASLGARVQNAQVFNQATGQWEPLDPAKSYSYAGYWYDQAPKKVGALVSANPVTAVKGAAGETLDGTEVVVNYLKTH
SANPGESRIRLVNPLPAAAYGSAEIQPLKGVSSAY
>Mature_595_residues
MQKGILSRAVLATFAAGAITAAFPVAGQTLATTAATLPVQKPAPDSKTMKLTLLQMGDIHGHMIPRAHLRNDGNGAMLGG
LAYMYSKIQEIRARNPNTLLFNTGDTIQGSAEALYTKGQALVDVLDRFGVVGFAPGNWDYLYGAERFVELFGSGRWGAVA
ANVYYDPAIYPDKAGQTVLPPYRILTVNGLKIGVMGLSTERITLAPGPFATAGLIATSEGQEVPGVIDTLRNQEKVDLVI
LVSEFGLAKNVHFAENFPGIDVVLSADMHEETRDPITTSNGTIISEVGQDGTRLGQIDLDIRDGKIASWKYTFNTIDTST
IRPNPVIAQLVAEKRKEFVAGKFFKKHVNPFNGMVLKTPIDKVVGKADVALHRSNYSDAAVPGAIEGTSHNFIADAFREQ
AGADVGHMRGFRYGTHIAPGDIKLDDLYHYIAIGPQVAKTTITGQMLKDDLENNAAGSFNSDIFKWTGGWQQALSGVRYD
LDLYASLGARVQNAQVFNQATGQWEPLDPAKSYSYAGYWYDQAPKKVGALVSANPVTAVKGAAGETLDGTEVVVNYLKTH
SANPGESRIRLVNPLPAAAYGSAEIQPLKGVSSAY

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Drosophila melanogaster, GI19921980, Length=417, Percent_Identity=22.7817745803357, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI161076508, Length=417, Percent_Identity=22.5419664268585, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 63894; Mature: 63894

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKGILSRAVLATFAAGAITAAFPVAGQTLATTAATLPVQKPAPDSKTMKLTLLQMGDIH
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCC
GHMIPRAHLRNDGNGAMLGGLAYMYSKIQEIRARNPNTLLFNTGDTIQGSAEALYTKGQA
CCCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCHH
LVDVLDRFGVVGFAPGNWDYLYGAERFVELFGSGRWGAVAANVYYDPAIYPDKAGQTVLP
HHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCC
PYRILTVNGLKIGVMGLSTERITLAPGPFATAGLIATSEGQEVPGVIDTLRNQEKVDLVI
CEEEEEECCEEEEEEEECCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEE
LVSEFGLAKNVHFAENFPGIDVVLSADMHEETRDPITTSNGTIISEVGQDGTRLGQIDLD
EEECCCCCCCCCHHCCCCCEEEEEECCCCHHHCCCCCCCCCEEEECCCCCCCEEEEEEEE
IRDGKIASWKYTFNTIDTSTIRPNPVIAQLVAEKRKEFVAGKFFKKHVNPFNGMVLKTPI
ECCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCH
DKVVGKADVALHRSNYSDAAVPGAIEGTSHNFIADAFREQAGADVGHMRGFRYGTHIAPG
HHHHCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
DIKLDDLYHYIAIGPQVAKTTITGQMLKDDLENNAAGSFNSDIFKWTGGWQQALSGVRYD
CEEHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCEEC
LDLYASLGARVQNAQVFNQATGQWEPLDPAKSYSYAGYWYDQAPKKVGALVSANPVTAVK
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHCCCCCEEECCCCCHHHCCEEECCCCEEEC
GAAGETLDGTEVVVNYLKTHSANPGESRIRLVNPLPAAAYGSAEIQPLKGVSSAY
CCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKGILSRAVLATFAAGAITAAFPVAGQTLATTAATLPVQKPAPDSKTMKLTLLQMGDIH
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCC
GHMIPRAHLRNDGNGAMLGGLAYMYSKIQEIRARNPNTLLFNTGDTIQGSAEALYTKGQA
CCCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCHH
LVDVLDRFGVVGFAPGNWDYLYGAERFVELFGSGRWGAVAANVYYDPAIYPDKAGQTVLP
HHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCC
PYRILTVNGLKIGVMGLSTERITLAPGPFATAGLIATSEGQEVPGVIDTLRNQEKVDLVI
CEEEEEECCEEEEEEEECCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEE
LVSEFGLAKNVHFAENFPGIDVVLSADMHEETRDPITTSNGTIISEVGQDGTRLGQIDLD
EEECCCCCCCCCHHCCCCCEEEEEECCCCHHHCCCCCCCCCEEEECCCCCCCEEEEEEEE
IRDGKIASWKYTFNTIDTSTIRPNPVIAQLVAEKRKEFVAGKFFKKHVNPFNGMVLKTPI
ECCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCH
DKVVGKADVALHRSNYSDAAVPGAIEGTSHNFIADAFREQAGADVGHMRGFRYGTHIAPG
HHHHCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
DIKLDDLYHYIAIGPQVAKTTITGQMLKDDLENNAAGSFNSDIFKWTGGWQQALSGVRYD
CEEHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCEEC
LDLYASLGARVQNAQVFNQATGQWEPLDPAKSYSYAGYWYDQAPKKVGALVSANPVTAVK
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHCCCCCEEECCCCCHHHCCEEECCCCEEEC
GAAGETLDGTEVVVNYLKTHSANPGESRIRLVNPLPAAAYGSAEIQPLKGVSSAY
CCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]