Click here to switch to the map view.

The map label for this gene is ydeH [H]

Identifier: 74317295

GI number: 74317295

Start: 1343320

End: 1344045

Strand: Reverse

Name: ydeH [H]

Synonym: Tbd_1277

Alternate gene names: 74317295

Gene position: 1344045-1343320 (Counterclockwise)

Preceding gene: 74317296

Following gene: 74317290

Centisome position: 46.19

GC content: 67.22

Gene sequence:

>726_bases
ATGGACTGGACGCGACGCATCCTGCGTTGCGCCGTACTGCACACGACGCCCGACGACGACGTGATCGACCCACAGGCGCA
TGCGCTGTGCCGCTTCGGTGGCTGGCTCGCGCACCATCACAAGCATCTCGAGTCGCTCGACGCGGCCGCCGCCGACCGTG
TCGACGTCGCGCACCGCGCGATGCACGACGCAATGCGGGCGATCTGCCTGTGCATCGTCCGCGGGGAGGCCGCGCCGGCC
GAGGAACTCGACGCCTTCGAGCAATCGCAGGAGGCGCTCATTCAGCTGCTGGCGACGTTCAAGACCCTGATCCTGTCCAA
GGCCGGGCGTCGCGATCCGCTGACGCAACTGCCGATGCGTCACAACATCGAAAGCGACTATCTCCTCTACCAGAAGGAAG
CGCGACGCAAGCGAAGCGCGCTCTACGTCGCGATGATTGACGTCGACCATTTCAAGCCGATCAACGATACCTATGGCCAT
CCCGCCGGCGACCGGGTCCTGCGCCACGTCGCGGAGACCTTGAAGCGGACGCTGCGCAGCGACGAGCCGCTCTATCGCTA
CGGCGGCGAAGAGTTTCTCTGGCTGCTGAAGTGCAACTCGCCCGACGAGGCCGGGCGCTCGGCGCAACGCGTGCTGGAGA
CCGTCGCGACGACGCCGGTGCCGGTCGACGACGCGGCGACCTCGCGCTGTACGAGGGCAAGCGCCGCGGCCGCAACCGCT
ACGTGA

Upstream 100 bases:

>100_bases
TTGCTGCGCAACCGGTCGCGCACCGATAGCCGAGGAAACGCCCCTGCTCCCAGAAACCGAACATCTCGTCGCCGAACTCG
ACGCCGCCGTCCAGGCCCAC

Downstream 100 bases:

>100_bases
TCGCGGACGCCTGAGGCGTCACGGCATCAATTCTTCCTTCGATTGCTGCATCGCCTGGGCGAGGCGCACGCCGCTTTCCC
AAGCGCGCTCGACGCCGACA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MDWTRRILRCAVLHTTPDDDVIDPQAHALCRFGGWLAHHHKHLESLDAAAADRVDVAHRAMHDAMRAICLCIVRGEAAPA
EELDAFEQSQEALIQLLATFKTLILSKAGRRDPLTQLPMRHNIESDYLLYQKEARRKRSALYVAMIDVDHFKPINDTYGH
PAGDRVLRHVAETLKRTLRSDEPLYRYGGEEFLWLLKCNSPDEAGRSAQRVLETVATTPVPVDDAATSRCTRASAAAATA
T

Sequences:

>Translated_241_residues
MDWTRRILRCAVLHTTPDDDVIDPQAHALCRFGGWLAHHHKHLESLDAAAADRVDVAHRAMHDAMRAICLCIVRGEAAPA
EELDAFEQSQEALIQLLATFKTLILSKAGRRDPLTQLPMRHNIESDYLLYQKEARRKRSALYVAMIDVDHFKPINDTYGH
PAGDRVLRHVAETLKRTLRSDEPLYRYGGEEFLWLLKCNSPDEAGRSAQRVLETVATTPVPVDDAATSRCTRASAAAATA
T
>Mature_241_residues
MDWTRRILRCAVLHTTPDDDVIDPQAHALCRFGGWLAHHHKHLESLDAAAADRVDVAHRAMHDAMRAICLCIVRGEAAPA
EELDAFEQSQEALIQLLATFKTLILSKAGRRDPLTQLPMRHNIESDYLLYQKEARRKRSALYVAMIDVDHFKPINDTYGH
PAGDRVLRHVAETLKRTLRSDEPLYRYGGEEFLWLLKCNSPDEAGRSAQRVLETVATTPVPVDDAATSRCTRASAAAATA
T

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=216, Percent_Identity=32.8703703703704, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI145693134, Length=110, Percent_Identity=38.1818181818182, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787262, Length=105, Percent_Identity=38.0952380952381, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082007, Length=97, Percent_Identity=36.0824742268041, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788381, Length=141, Percent_Identity=34.0425531914894, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=126, Percent_Identity=30.952380952381, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786584, Length=126, Percent_Identity=32.5396825396825, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 26986; Mature: 26986

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWTRRILRCAVLHTTPDDDVIDPQAHALCRFGGWLAHHHKHLESLDAAAADRVDVAHRA
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHDAMRAICLCIVRGEAAPAEELDAFEQSQEALIQLLATFKTLILSKAGRRDPLTQLPMR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHH
HNIESDYLLYQKEARRKRSALYVAMIDVDHFKPINDTYGHPAGDRVLRHVAETLKRTLRS
CCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DEPLYRYGGEEFLWLLKCNSPDEAGRSAQRVLETVATTPVPVDDAATSRCTRASAAAATA
CCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
T
C
>Mature Secondary Structure
MDWTRRILRCAVLHTTPDDDVIDPQAHALCRFGGWLAHHHKHLESLDAAAADRVDVAHRA
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHDAMRAICLCIVRGEAAPAEELDAFEQSQEALIQLLATFKTLILSKAGRRDPLTQLPMR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHH
HNIESDYLLYQKEARRKRSALYVAMIDVDHFKPINDTYGHPAGDRVLRHVAETLKRTLRS
CCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DEPLYRYGGEEFLWLLKCNSPDEAGRSAQRVLETVATTPVPVDDAATSRCTRASAAAATA
CCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503 [H]