Click here to switch to the map view.

The map label for this gene is rluB [H]

Identifier: 74317276

GI number: 74317276

Start: 1320068

End: 1320958

Strand: Direct

Name: rluB [H]

Synonym: Tbd_1258

Alternate gene names: 74317276

Gene position: 1320068-1320958 (Clockwise)

Preceding gene: 74317275

Following gene: 74317278

Centisome position: 45.37

GC content: 69.02

Gene sequence:

>891_bases
ATGGCCCGACCCGCATCCCCCATCCGCCGCTCGCCTAACGCTCCCCAGGGGCGCGCGGCGAGCCGCCTGCAACAGGCGAC
CGCGCCCGAGGCTGCGAGCGAGCGTCTGCACAAGGCGCTCGCCTCAGCCGGGCTCGGCTCGCGCCGCGAGATGGAGGAGT
GGATCGCCGAAGGGCGCGTGCAGGTCAATGGCGAGACGGCGACCGTCGGCAGCAAGGTCGGTCCGCGCGACGTCGTCAAG
GTCAGCGGCCGTCGCGTCTATCTGCGCTTCGACGAAAAACGCACGCGCATCCTGATCTATCACAAGTCCGAAGGGGAAAT
CGTCAGTCGCGACGACCCCGGCGGCCGCGCGACCGTGTTCGACGCCTTGCCCAGGTTGCGCGGGTCGAAGTGGATCGCGG
TCGGCCGGCTCGACTACAACACCGACGGTCTCATGATCTTCACGACCTCGGGCGATCTCGCCAATCGGCTCATGCATCCG
CGCTTCGAGGTCGAACGCGAATACGCGGTGCGCGTGCTCGGCGAACTGACCGAGGACATGATCGCCGAGCTGCTGGCAGG
GGTCGAACTCGACGACGGCCCGGCGCAGTTGCAGAGCTGTTTCGCCGCCGGGGGCGAGGGCGCCAACCGCTGGTATCGCG
TCGTCATCAAGGAAGGCCGCAAGCGTGAGGTGCGGCGCCTGTTCGAGCACTTGGGGCTGACGGTCTCGCGGCTGACGCGT
ATCCGCTTCGGCCCGATCGTGTTGCCGCCGCAACTGCGGCGCGGCCAGAAGACCGAACTCGACGACGACCTCGTCGCGCA
GGTCATGCGCTGGGCCGGAATGGAAGCGAGCCCGCGCCGCAAGACGCGCGGCGTCCCCGACAAGCACACGCGCAAGCCGC
GCATCCGCTGA

Upstream 100 bases:

>100_bases
CTGCGCGAGGAATTGCAGGACAATGACGCCGCGCCGGCATTCGGCGCAGTGATCGAAACCGCCGCGCCCGCCGGCGCGGT
CACCGAGAATATCTGACTTC

Downstream 100 bases:

>100_bases
CGCGCCTGTCCGGCGTCGCCGGCCGCCGGTGGGCCGGGACGCCGCCGCCTTAAGCTGTAGTGCGGGCAGGGCGCCGCAGC
CGTAGCCTTCGACCGGGCAC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MARPASPIRRSPNAPQGRAASRLQQATAPEAASERLHKALASAGLGSRREMEEWIAEGRVQVNGETATVGSKVGPRDVVK
VSGRRVYLRFDEKRTRILIYHKSEGEIVSRDDPGGRATVFDALPRLRGSKWIAVGRLDYNTDGLMIFTTSGDLANRLMHP
RFEVEREYAVRVLGELTEDMIAELLAGVELDDGPAQLQSCFAAGGEGANRWYRVVIKEGRKREVRRLFEHLGLTVSRLTR
IRFGPIVLPPQLRRGQKTELDDDLVAQVMRWAGMEASPRRKTRGVPDKHTRKPRIR

Sequences:

>Translated_296_residues
MARPASPIRRSPNAPQGRAASRLQQATAPEAASERLHKALASAGLGSRREMEEWIAEGRVQVNGETATVGSKVGPRDVVK
VSGRRVYLRFDEKRTRILIYHKSEGEIVSRDDPGGRATVFDALPRLRGSKWIAVGRLDYNTDGLMIFTTSGDLANRLMHP
RFEVEREYAVRVLGELTEDMIAELLAGVELDDGPAQLQSCFAAGGEGANRWYRVVIKEGRKREVRRLFEHLGLTVSRLTR
IRFGPIVLPPQLRRGQKTELDDDLVAQVMRWAGMEASPRRKTRGVPDKHTRKPRIR
>Mature_295_residues
ARPASPIRRSPNAPQGRAASRLQQATAPEAASERLHKALASAGLGSRREMEEWIAEGRVQVNGETATVGSKVGPRDVVKV
SGRRVYLRFDEKRTRILIYHKSEGEIVSRDDPGGRATVFDALPRLRGSKWIAVGRLDYNTDGLMIFTTSGDLANRLMHPR
FEVEREYAVRVLGELTEDMIAELLAGVELDDGPAQLQSCFAAGGEGANRWYRVVIKEGRKREVRRLFEHLGLTVSRLTRI
RFGPIVLPPQLRRGQKTELDDDLVAQVMRWAGMEASPRRKTRGVPDKHTRKPRIR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=54.7413793103448, Blast_Score=243, Evalue=9e-66,
Organism=Escherichia coli, GI1790453, Length=246, Percent_Identity=30.8943089430894, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081838, Length=192, Percent_Identity=29.6875, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788510, Length=218, Percent_Identity=29.3577981651376, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33221; Mature: 33089

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPASPIRRSPNAPQGRAASRLQQATAPEAASERLHKALASAGLGSRREMEEWIAEGRV
CCCCCCCHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEE
QVNGETATVGSKVGPRDVVKVSGRRVYLRFDEKRTRILIYHKSEGEIVSRDDPGGRATVF
EECCCEEECCCCCCCHHHEEECCCEEEEEECCCCEEEEEEECCCCCEECCCCCCCCCHHH
DALPRLRGSKWIAVGRLDYNTDGLMIFTTSGDLANRLMHPRFEVEREYAVRVLGELTEDM
HHHHHHCCCCEEEEEEEEECCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IAELLAGVELDDGPAQLQSCFAAGGEGANRWYRVVIKEGRKREVRRLFEHLGLTVSRLTR
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHH
IRFGPIVLPPQLRRGQKTELDDDLVAQVMRWAGMEASPRRKTRGVPDKHTRKPRIR
HHCCCEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARPASPIRRSPNAPQGRAASRLQQATAPEAASERLHKALASAGLGSRREMEEWIAEGRV
CCCCCCHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEE
QVNGETATVGSKVGPRDVVKVSGRRVYLRFDEKRTRILIYHKSEGEIVSRDDPGGRATVF
EECCCEEECCCCCCCHHHEEECCCEEEEEECCCCEEEEEEECCCCCEECCCCCCCCCHHH
DALPRLRGSKWIAVGRLDYNTDGLMIFTTSGDLANRLMHPRFEVEREYAVRVLGELTEDM
HHHHHHCCCCEEEEEEEEECCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IAELLAGVELDDGPAQLQSCFAAGGEGANRWYRVVIKEGRKREVRRLFEHLGLTVSRLTR
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHH
IRFGPIVLPPQLRRGQKTELDDDLVAQVMRWAGMEASPRRKTRGVPDKHTRKPRIR
HHCCCEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]