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The map label for this gene is fliA [H]

Identifier: 74317267

GI number: 74317267

Start: 1311870

End: 1312610

Strand: Direct

Name: fliA [H]

Synonym: Tbd_1249

Alternate gene names: 74317267

Gene position: 1311870-1312610 (Clockwise)

Preceding gene: 74317266

Following gene: 74317268

Centisome position: 45.08

GC content: 64.78

Gene sequence:

>741_bases
ATGTACACCGCAAGCGGCAAGACCGCGAAGAGCGAAAAAAACGATCTGCTCGAGCAGTACATGCCGATGGTCAAGCGGCT
CGCGCACCACATGATGGGCCGGCTGCCGCCCAGCGTCGAGGAGGACGATCTTGTCCAGGCCGGCATGATCGGCCTGCTCG
ACGCCGTCAGCCGTTACGACGAGGCGCAGAGCGCGCAGTTCGAGGCCTACGCGATCCAGCGCATCCGCGGTTCGATGATC
GACGAGTTGCGGCAGTCGGACTGGATGCCGCGCAGCGCTCGGCAATCGATGCGCAAGATCGAGCAGGCCATCGCCGCGCT
GCAGCACCAGTTGGGGCGTCAGCCGAGCGAGGGCGAAATCGCCGAGGCGATGAAGATCCCGCTCGCCGACTACCAGTCGA
TGCTTGGCGATGCGCGCGGCCACCAGTTGCTTTACTTCGAGGATTTCGGCGAGTCCGACGAGGACGACTCCTTCCTCGAC
AAGCAGTCCGCCGACGAGGCGACTATGCCGCTGCCGCAGCTGCTCGACGCCAACCTCAGGGCCTGCATCATCGCCGGCAT
CGAGAACCTGCCCGAGCGCGAGCAGCTCCTGATGTCGCTGTATTACGAACAGGAACTCAACCTGCGCGAGATCGCCGAGG
TCTTCGGCGTCAGCGAGTCGCGCATCTGCCAGCTGCACGGCCAGGCGATTGCGCGGCTGCGCGCCCGGCTCAAGGAAGAT
TCGTGGATCGCAGCAGCCTGA

Upstream 100 bases:

>100_bases
TGCGCTGGCCGCAACCCGCGAGCCTCGGCGGACTGGGCGGGTTCATGCAACGCGTGATCCAGGGCGGACGCCTCGCAGAA
GCCTGCAGGAGAGGGTGAAG

Downstream 100 bases:

>100_bases
TCGGGCTCGCGCTCGGCGGCGCCGCGATCCTCGGCGGCCAGGCGATCGAAGGCGGTCACCTCGGGCTGTTCGTTCAGCCG
GCGGCCCTGCTCATCGTCGT

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MYTASGKTAKSEKNDLLEQYMPMVKRLAHHMMGRLPPSVEEDDLVQAGMIGLLDAVSRYDEAQSAQFEAYAIQRIRGSMI
DELRQSDWMPRSARQSMRKIEQAIAALQHQLGRQPSEGEIAEAMKIPLADYQSMLGDARGHQLLYFEDFGESDEDDSFLD
KQSADEATMPLPQLLDANLRACIIAGIENLPEREQLLMSLYYEQELNLREIAEVFGVSESRICQLHGQAIARLRARLKED
SWIAAA

Sequences:

>Translated_246_residues
MYTASGKTAKSEKNDLLEQYMPMVKRLAHHMMGRLPPSVEEDDLVQAGMIGLLDAVSRYDEAQSAQFEAYAIQRIRGSMI
DELRQSDWMPRSARQSMRKIEQAIAALQHQLGRQPSEGEIAEAMKIPLADYQSMLGDARGHQLLYFEDFGESDEDDSFLD
KQSADEATMPLPQLLDANLRACIIAGIENLPEREQLLMSLYYEQELNLREIAEVFGVSESRICQLHGQAIARLRARLKED
SWIAAA
>Mature_246_residues
MYTASGKTAKSEKNDLLEQYMPMVKRLAHHMMGRLPPSVEEDDLVQAGMIGLLDAVSRYDEAQSAQFEAYAIQRIRGSMI
DELRQSDWMPRSARQSMRKIEQAIAALQHQLGRQPSEGEIAEAMKIPLADYQSMLGDARGHQLLYFEDFGESDEDDSFLD
KQSADEATMPLPQLLDANLRACIIAGIENLPEREQLLMSLYYEQELNLREIAEVFGVSESRICQLHGQAIARLRARLKED
SWIAAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=240, Percent_Identity=49.1666666666667, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=25.2136752136752, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27821; Mature: 27821

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTASGKTAKSEKNDLLEQYMPMVKRLAHHMMGRLPPSVEEDDLVQAGMIGLLDAVSRYD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EAQSAQFEAYAIQRIRGSMIDELRQSDWMPRSARQSMRKIEQAIAALQHQLGRQPSEGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AEAMKIPLADYQSMLGDARGHQLLYFEDFGESDEDDSFLDKQSADEATMPLPQLLDANLR
HHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHCHHH
ACIIAGIENLPEREQLLMSLYYEQELNLREIAEVFGVSESRICQLHGQAIARLRARLKED
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
SWIAAA
CCCCCC
>Mature Secondary Structure
MYTASGKTAKSEKNDLLEQYMPMVKRLAHHMMGRLPPSVEEDDLVQAGMIGLLDAVSRYD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EAQSAQFEAYAIQRIRGSMIDELRQSDWMPRSARQSMRKIEQAIAALQHQLGRQPSEGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AEAMKIPLADYQSMLGDARGHQLLYFEDFGESDEDDSFLDKQSADEATMPLPQLLDANLR
HHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHCHHH
ACIIAGIENLPEREQLLMSLYYEQELNLREIAEVFGVSESRICQLHGQAIARLRARLKED
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
SWIAAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]