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The map label for this gene is gltA [H]

Identifier: 74317205

GI number: 74317205

Start: 1245927

End: 1247222

Strand: Direct

Name: gltA [H]

Synonym: Tbd_1187

Alternate gene names: 74317205

Gene position: 1245927-1247222 (Clockwise)

Preceding gene: 74317204

Following gene: 161899034

Centisome position: 42.82

GC content: 64.2

Gene sequence:

>1296_bases
ATGAAGAAAACCGTCACCCTCACTTTCAGCGACGGCAGTCCCGCGATTGAATTGCCGATCGAAGAGGGCACCTACGGCAA
GCCCGTGATCGACATCCGCGCCCTCGGCGCACACGGCTATTTCACGCACGACCCGGGCTTCACCGCGACTTCGAGCTGTA
CCTCGTCGATCACCTACATCGACGGCGACGAAGGCCTGCTGTACTACCGGGGCTACCCGATCGACCAACTCGCCTACCAG
TCGGACTTCATGGAAGTCAGCTACCTGCTGCTGCACGGCGAACTGCCGACGGCGGAACAGAAGGCCGGGTTCGAGCAGTC
GATCCGTCACCACACGCTGCTGCACGACCAGATCGAAAACGTGTTCCGCGGCTTTCGCCGCAGCGCGCACCCGATGGCGG
TGATGATCGGCGTGACCGGCGCGATGTCGGCCTTCTATCACGCCGGCCTCGACAACTTCAATCCCGAGCACCGCGAGATC
GTCGCCCACCGCCTGATCGCCAAGATCCCGACCGTCGCCGCGTGGGCGTACAAGTTCAACGAGGGCCAGCCCTTCGTCTA
CCCGCAGAACCGCCTGTCCTACGCCGAGAACTTCATGTACATGATGTTCTCGAGCCCCTGCGAGGAATACGTGCCCAACC
CGGTGCTGGCGCGCGCGCTCGACCGCATCTTCATCCTGCACGCCGACCACGAGCAGAACGCGTCGACCTCGACGGTACGG
CTCGCCGGGTCGAGCGGCGCCAACCCCTACGCCTGCATCGCGAGCGGCATGGCGTCGCTGTGGGGGCCGCTGCACGGCGG
CGCCAACGAAGCCGTGCTCCGCATGCTCGAGGAGATCGACGACGCGTCCAAGATTCCCGATTTCATCAAGCGCGCGAAGG
ACAAATCCGATCCTTTCCGCCTGATGGGCTTCGGTCATCGCGTCTACAAGAACATGGACCCGCGCGCGGCGATCATCCGC
CGCACCTGCTACGAGGTGCTCGACGAGCTTGGCCTGCGTGACGATCCGCAGTTCAAGATCGCGCTCGAGCTCGAGCGCAT
CGCGCTTGAGGACGAGTACTTCATCGAGCGCAAACTCTATCCGAACGTCGACTTCTATTCGGGCATCATCTTCCGCGCGA
TGGGTATTCCGACCAGCATGTTCACGGCATTGTTCGCGATGGCGCGCACGGCCGGCTGGGTCGCGCAGTGGCACGAGATG
TTATCCGACCCGGCGCACAAGATCGGCCGCCCGCGCCAGCTCTACGCGGGTCCGGCGCGACGCGACTTCGTGCCGCTCGA
CCAGCGCAGGGCCTGA

Upstream 100 bases:

>100_bases
CGGACATGGAAATCCTCGACAGGCTGCACGGCCGCTCGGCGCCGGACGATGCCGACGTCGACGCGCTCATTCAACGCCTG
AAAAATTTTCGAGAAGCAAA

Downstream 100 bases:

>100_bases
GCTCGCCGCCGGCATGAGCGCGCCCGACTGGCTTTCGACCTCGCCGCTCTACTCGGGCAACGCGGCGTATCTCGAGCAGT
TCGGCGACGCCGCCCTCGCG

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKKTVTLTFSDGSPAIELPIEEGTYGKPVIDIRALGAHGYFTHDPGFTATSSCTSSITYIDGDEGLLYYRGYPIDQLAYQ
SDFMEVSYLLLHGELPTAEQKAGFEQSIRHHTLLHDQIENVFRGFRRSAHPMAVMIGVTGAMSAFYHAGLDNFNPEHREI
VAHRLIAKIPTVAAWAYKFNEGQPFVYPQNRLSYAENFMYMMFSSPCEEYVPNPVLARALDRIFILHADHEQNASTSTVR
LAGSSGANPYACIASGMASLWGPLHGGANEAVLRMLEEIDDASKIPDFIKRAKDKSDPFRLMGFGHRVYKNMDPRAAIIR
RTCYEVLDELGLRDDPQFKIALELERIALEDEYFIERKLYPNVDFYSGIIFRAMGIPTSMFTALFAMARTAGWVAQWHEM
LSDPAHKIGRPRQLYAGPARRDFVPLDQRRA

Sequences:

>Translated_431_residues
MKKTVTLTFSDGSPAIELPIEEGTYGKPVIDIRALGAHGYFTHDPGFTATSSCTSSITYIDGDEGLLYYRGYPIDQLAYQ
SDFMEVSYLLLHGELPTAEQKAGFEQSIRHHTLLHDQIENVFRGFRRSAHPMAVMIGVTGAMSAFYHAGLDNFNPEHREI
VAHRLIAKIPTVAAWAYKFNEGQPFVYPQNRLSYAENFMYMMFSSPCEEYVPNPVLARALDRIFILHADHEQNASTSTVR
LAGSSGANPYACIASGMASLWGPLHGGANEAVLRMLEEIDDASKIPDFIKRAKDKSDPFRLMGFGHRVYKNMDPRAAIIR
RTCYEVLDELGLRDDPQFKIALELERIALEDEYFIERKLYPNVDFYSGIIFRAMGIPTSMFTALFAMARTAGWVAQWHEM
LSDPAHKIGRPRQLYAGPARRDFVPLDQRRA
>Mature_431_residues
MKKTVTLTFSDGSPAIELPIEEGTYGKPVIDIRALGAHGYFTHDPGFTATSSCTSSITYIDGDEGLLYYRGYPIDQLAYQ
SDFMEVSYLLLHGELPTAEQKAGFEQSIRHHTLLHDQIENVFRGFRRSAHPMAVMIGVTGAMSAFYHAGLDNFNPEHREI
VAHRLIAKIPTVAAWAYKFNEGQPFVYPQNRLSYAENFMYMMFSSPCEEYVPNPVLARALDRIFILHADHEQNASTSTVR
LAGSSGANPYACIASGMASLWGPLHGGANEAVLRMLEEIDDASKIPDFIKRAKDKSDPFRLMGFGHRVYKNMDPRAAIIR
RTCYEVLDELGLRDDPQFKIALELERIALEDEYFIERKLYPNVDFYSGIIFRAMGIPTSMFTALFAMARTAGWVAQWHEM
LSDPAHKIGRPRQLYAGPARRDFVPLDQRRA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=404, Percent_Identity=27.2277227722772, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786939, Length=413, Percent_Identity=65.8595641646489, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1786527, Length=383, Percent_Identity=31.5926892950392, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=383, Percent_Identity=25.8485639686684, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=346, Percent_Identity=29.1907514450867, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6325257, Length=360, Percent_Identity=28.6111111111111, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=370, Percent_Identity=26.2162162162162, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24640126, Length=401, Percent_Identity=26.4339152119701, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640124, Length=401, Percent_Identity=26.4339152119701, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356863, Length=406, Percent_Identity=26.8472906403941, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48625; Mature: 48625

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVTLTFSDGSPAIELPIEEGTYGKPVIDIRALGAHGYFTHDPGFTATSSCTSSITYI
CCEEEEEEECCCCCEEEEECCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCEEEE
DGDEGLLYYRGYPIDQLAYQSDFMEVSYLLLHGELPTAEQKAGFEQSIRHHTLLHDQIEN
ECCCCEEEECCCCHHHHHHHHHHHHEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHH
VFRGFRRSAHPMAVMIGVTGAMSAFYHAGLDNFNPEHREIVAHRLIAKIPTVAAWAYKFN
HHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
EGQPFVYPQNRLSYAENFMYMMFSSPCEEYVPNPVLARALDRIFILHADHEQNASTSTVR
CCCCEECCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHEEEEECCCCCCCCCCEEE
LAGSSGANPYACIASGMASLWGPLHGGANEAVLRMLEEIDDASKIPDFIKRAKDKSDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCHHHHHHHCCCCCCEE
LMGFGHRVYKNMDPRAAIIRRTCYEVLDELGLRDDPQFKIALELERIALEDEYFIERKLY
EHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHCCCHHHHHHHCC
PNVDFYSGIIFRAMGIPTSMFTALFAMARTAGWVAQWHEMLSDPAHKIGRPRQLYAGPAR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCCCC
RDFVPLDQRRA
CCCCCCCCCCC
>Mature Secondary Structure
MKKTVTLTFSDGSPAIELPIEEGTYGKPVIDIRALGAHGYFTHDPGFTATSSCTSSITYI
CCEEEEEEECCCCCEEEEECCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCEEEE
DGDEGLLYYRGYPIDQLAYQSDFMEVSYLLLHGELPTAEQKAGFEQSIRHHTLLHDQIEN
ECCCCEEEECCCCHHHHHHHHHHHHEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHH
VFRGFRRSAHPMAVMIGVTGAMSAFYHAGLDNFNPEHREIVAHRLIAKIPTVAAWAYKFN
HHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
EGQPFVYPQNRLSYAENFMYMMFSSPCEEYVPNPVLARALDRIFILHADHEQNASTSTVR
CCCCEECCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHEEEEECCCCCCCCCCEEE
LAGSSGANPYACIASGMASLWGPLHGGANEAVLRMLEEIDDASKIPDFIKRAKDKSDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCHHHHHHHCCCCCCEE
LMGFGHRVYKNMDPRAAIIRRTCYEVLDELGLRDDPQFKIALELERIALEDEYFIERKLY
EHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHCCCHHHHHHHCC
PNVDFYSGIIFRAMGIPTSMFTALFAMARTAGWVAQWHEMLSDPAHKIGRPRQLYAGPAR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCCCC
RDFVPLDQRRA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2507528; 10984043 [H]