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The map label for this gene is nuoN

Identifier: 74317173

GI number: 74317173

Start: 1210086

End: 1211537

Strand: Direct

Name: nuoN

Synonym: Tbd_1155

Alternate gene names: 74317173

Gene position: 1210086-1211537 (Clockwise)

Preceding gene: 74317172

Following gene: 74317174

Centisome position: 41.59

GC content: 64.19

Gene sequence:

>1452_bases
ATGAACATGCCCCTGAGCCTGATGCAATTTGCCCCCGCGCTGCCCGAGATTTTCGTGCTGGCGATGGTTTCGCTGATCCT
CGTGATCGATGCCGCGGTCGGCGACGGCAAGCGCTACCTGGCCTACGGCCTGTCGCTCGTGACGCTGGCCGGCGCCGCCT
TCCTGACCGTGCGCGATTTCTCGACCCTGCCGGTGCTCGCCCTGGGTGGCCTGTTCATCGACGATCCGCTGTCGGACGTC
CTCAAGCTCTTCCTCTACCTGACGGTCGCGATCGTGCTCGTCTACTCGCGCGATTACCTCCGGGTCCGTGGCCTGTACAA
GGGCGAATTCTTCGTGCTCGCGCTGTTCGCGCTGCTCGGCATGATGGTGATGGTGTCGGCGAGCCATTTCCTCACGCTCT
ATCTCGGCCTCGAACTCTTGTCGCTGTCGCTGTACGCGATGGTGGCATTGCAGCGCGATTCGAGCGTCGCGACCGAGGCC
GCGATGAAGTATTTCGTGCTCGGTGCGCTCGCGTCGGGCATGCTGCTCTACGGCATGTCGATGGTTTACGGCGTGACGGG
CTCGCTCGCGCTCGGCGACATCGCCATCGTGCTGCAGGACGGGACCGACCTGCGCATCCCGCTGGTGTTCGGCATCGTGT
TCGTTGTCGCCGGCCTGGCCTTCAAGCTCGGCGCGGTTCCTTTCCACATGTGGGTGCCCGACGTCTACCACGGCGCGCCG
ACCGCGATGACGCTCTTCGTCGGCTCGGCGCCGAAGATCGCCGCCTTCGCGTTCGTCGTGCGCGTCCTCGGGCAGGGACT
GGAGTCGCAGGTCAGCGAATGGCGGGACATGCTCGTGATCCTCGCGGTGTTGTCGATGGCGATCGGCAATATCGCGGCCA
TCGCGCAGAGCAATCTCAAACGCATGTTCGCCTATTCGACCATCTCGCACATGGGCTTCATGCTGCTCGGCGTGCTTGCC
GGCTCGCAGAACGGCTACGGCGCCGCGATGTTCTACGTGCTGGTCTATACGCTGATGACGCTCGGCGGCTTCGGCATGAT
CCTGCTGCTGTCGCGGGCCGGCTTCGAGTCCGATCGGCTCGACGACTTTAAGGGACTGAACCGGCGCAGCCCGTGGCTCG
CCTTCGTCATGCTGCTGCTGATGTTCTCGATGACCGGCATTCCGCCGACGGTCGGCTTCTACGCCAAACTCGCGGTGCTC
CAGGCCGTCGTCGAAATCGGTTATGTCTGGCTCGCGGTGGCCGCCGTGCTGTTCTCGCTCGTCGGCGCATTCTATTATCT
GCGTATCGTCAAGCTGATGTATTTCGACACGCCGCAGGACACCGCACCGATCGCCCCCGGCATCGACGCGCGGCTCCTCA
TGTCGGCGAACGGCCTCGCGGTTCTGGCGCTCGGGATTCTGCCGCAACCGCTCATGGCCGTGTGCGTGCAGGCGATCGGT
ACCTCGTTCTAG

Upstream 100 bases:

>100_bases
ATGGGGCTCTATCCCAAACCCTTTACCGACGTCATGCATGCCTCTGTCGTCGACCTGCTGGCGCACGCCGCGCAGAGCAA
GCTTCCGTGAGCGCGACGCG

Downstream 100 bases:

>100_bases
ATCTCCCCTCCGGCCTGCGCGCCGGATCCATCTCGATTTTTCGGATTCATCGTGAATTTCTCGACTGTTTTATTGCTGCT
CCTGGCCTTTGTGGCCGCCA

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNMPLSLMQFAPALPEIFVLAMVSLILVIDAAVGDGKRYLAYGLSLVTLAGAAFLTVRDFSTLPVLALGGLFIDDPLSDV
LKLFLYLTVAIVLVYSRDYLRVRGLYKGEFFVLALFALLGMMVMVSASHFLTLYLGLELLSLSLYAMVALQRDSSVATEA
AMKYFVLGALASGMLLYGMSMVYGVTGSLALGDIAIVLQDGTDLRIPLVFGIVFVVAGLAFKLGAVPFHMWVPDVYHGAP
TAMTLFVGSAPKIAAFAFVVRVLGQGLESQVSEWRDMLVILAVLSMAIGNIAAIAQSNLKRMFAYSTISHMGFMLLGVLA
GSQNGYGAAMFYVLVYTLMTLGGFGMILLLSRAGFESDRLDDFKGLNRRSPWLAFVMLLLMFSMTGIPPTVGFYAKLAVL
QAVVEIGYVWLAVAAVLFSLVGAFYYLRIVKLMYFDTPQDTAPIAPGIDARLLMSANGLAVLALGILPQPLMAVCVQAIG
TSF

Sequences:

>Translated_483_residues
MNMPLSLMQFAPALPEIFVLAMVSLILVIDAAVGDGKRYLAYGLSLVTLAGAAFLTVRDFSTLPVLALGGLFIDDPLSDV
LKLFLYLTVAIVLVYSRDYLRVRGLYKGEFFVLALFALLGMMVMVSASHFLTLYLGLELLSLSLYAMVALQRDSSVATEA
AMKYFVLGALASGMLLYGMSMVYGVTGSLALGDIAIVLQDGTDLRIPLVFGIVFVVAGLAFKLGAVPFHMWVPDVYHGAP
TAMTLFVGSAPKIAAFAFVVRVLGQGLESQVSEWRDMLVILAVLSMAIGNIAAIAQSNLKRMFAYSTISHMGFMLLGVLA
GSQNGYGAAMFYVLVYTLMTLGGFGMILLLSRAGFESDRLDDFKGLNRRSPWLAFVMLLLMFSMTGIPPTVGFYAKLAVL
QAVVEIGYVWLAVAAVLFSLVGAFYYLRIVKLMYFDTPQDTAPIAPGIDARLLMSANGLAVLALGILPQPLMAVCVQAIG
TSF
>Mature_483_residues
MNMPLSLMQFAPALPEIFVLAMVSLILVIDAAVGDGKRYLAYGLSLVTLAGAAFLTVRDFSTLPVLALGGLFIDDPLSDV
LKLFLYLTVAIVLVYSRDYLRVRGLYKGEFFVLALFALLGMMVMVSASHFLTLYLGLELLSLSLYAMVALQRDSSVATEA
AMKYFVLGALASGMLLYGMSMVYGVTGSLALGDIAIVLQDGTDLRIPLVFGIVFVVAGLAFKLGAVPFHMWVPDVYHGAP
TAMTLFVGSAPKIAAFAFVVRVLGQGLESQVSEWRDMLVILAVLSMAIGNIAAIAQSNLKRMFAYSTISHMGFMLLGVLA
GSQNGYGAAMFYVLVYTLMTLGGFGMILLLSRAGFESDRLDDFKGLNRRSPWLAFVMLLLMFSMTGIPPTVGFYAKLAVL
QAVVEIGYVWLAVAAVLFSLVGAFYYLRIVKLMYFDTPQDTAPIAPGIDARLLMSANGLAVLALGILPQPLMAVCVQAIG
TSF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=316, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693160, Length=383, Percent_Identity=38.1201044386423, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788614, Length=372, Percent_Identity=23.9247311827957, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788613, Length=398, Percent_Identity=26.6331658291457, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788827, Length=337, Percent_Identity=25.2225519287834, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788831, Length=334, Percent_Identity=25.1497005988024, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_THIDA (Q3SJP4)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314913.1
- STRING:   Q3SJP4
- GeneID:   3673227
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1155
- NMPDR:   fig|292415.3.peg.1155
- HOGENOM:   HBG747830
- OMA:   MDMNTLL
- PhylomeDB:   Q3SJP4
- ProtClustDB:   PRK05777
- BioCyc:   TDEN292415:TBD_1155-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 51979; Mature: 51979

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b2ed01c)-; HASH(0x1ab6d5d0)-; HASH(0x1ab316d8)-; HASH(0x1ab25ea0)-; HASH(0x1ab0cd40)-; HASH(0x1ab6fa54)-; HASH(0x1ab64288)-; HASH(0x1b5f5c60)-; HASH(0x1ab6b05c)-; HASH(0x1ab214f4)-; HASH(0x1ab643b4)-; HASH(0x1ab2f4ac)-; HASH(0x1b5764ac)-; HASH(0x1ab26104)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCH
>Mature Secondary Structure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CCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA