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The map label for this gene is nuoM [H]

Identifier: 74317172

GI number: 74317172

Start: 1208591

End: 1210075

Strand: Direct

Name: nuoM [H]

Synonym: Tbd_1154

Alternate gene names: 74317172

Gene position: 1208591-1210075 (Clockwise)

Preceding gene: 74317171

Following gene: 74317173

Centisome position: 41.54

GC content: 62.15

Gene sequence:

>1485_bases
ATGTTCGGACAGTCGATTCTTTCCCTCGTCATCTGGGTCCCGATTCTTGCAGGTGCAGCGGTCCTCGCCACGGGCTCCGA
CCGCAACGCGCCGCTCGCGCGCTGGCTTGCGCTGCTGGGGGCTGTGCTTGGCCTTGCGGTCGCGATCCCGCTGGTTACGG
GTTTCGACAACGCGACTTCGGCGATGCAGTTCGTCGAGAAGAAAAGCTGGATTCCGGCGTTCAACGTCTTCTACCACCTG
GGCGTCGACGGCATTTCGATGCCGCTGATCCTGCTGAACAGCTTCGTCACCGTGCTCGTCGTCATCGCCGGCTGGCAGGT
GATCCAGGACAAGGTCGCGCAGTACATGGCAGCGTTCCTGATCATGTCGGGGCTCATCAACGGCGCCTTCGCTGCGCTCG
ACGGCATCCTGTTCTACGTCTTCTTCGAAGGCATGCTGATCCCGCTCTACCTGATCGTCGGTGTCTGGGGCGGACCGAAC
CGCGTCTATGCCGCGTTCAAGTTCTTTCTCTATACCTTGCTCGGCTCGCTGCTGATGCTGGTGTCGCTGATCTATCTCTA
TTTCCAGTCCGGCGGCAGCTTCGACATTCAGAGCTGGTACACCACGCCGCTCAGCATGACCGCGCAGACGCTGATGTTCT
TCGCCTTTCTGCTCGCATTCGGCGTCAAGGTCCCGATGTGGCCGGTGCACACCTGGTTGCCCGACGCGCACGTCGAGGCG
CCGACCGGCGGTTCGGTCGTGCTCGCGGCGATCACGCTCAAGGTCGGCGCCTACGGGCTCGTGCGCTTCCTGTTGCCCAT
CGTTCCCGATGCGGCCCACGAATACGCCTGGTTCGTCATCACCCTGTCGTTGATCGCGGTCGCGTACATCGGCCTCGTTG
CGCTCGTGCAGGCCGACATGAAGAAGCTCATCGCCTATTCGTCGATCGCCCACATGGGATTCGTGACCCTCGGTTTCTTC
CTGTTCAACCCGCTCGGCTGGGAGGGCGGCCTGGTGCAGATGATCTCCCACGGCTTCGTCTCGGCCGCGCTGTTCCTCTG
CATCGGCGTGATGTACGACCGCCTGCACTCGCGTCAGATCAAGGATTACGGCGGCCTCGTCAATCGCATGCCGGTGTTCG
CGGCCTTCTTCATGCTGTTCGCGATGGCCAATTCGGGGCTTCCGGCGACCAGTGGTTTCGTCGGCGAATTCATGGTCATC
ATGGCAGCCACCGAGGTCAATTTCTGGTTCGCCTTCGTCGCCGCGACGACGCTGATCTTCGGCGCGGCCTACACACTCTG
GATGTACAAACGCGTCGTTTTCGGCAGCGTGACCAATTCGCGCGTCGCCCAGATGCGCGACGTGAACGCGCGCGAGATCC
TGCTGCTCGGCCTCCTTGCCCTGTTCGTGCTGGGGATGGGGCTCTATCCCAAACCCTTTACCGACGTCATGCATGCCTCT
GTCGTCGACCTGCTGGCGCACGCCGCGCAGAGCAAGCTTCCGTGA

Upstream 100 bases:

>100_bases
TTGTCCAGTCCGGCTACATCTACCATTACGCCTTCGCCATGCTGATCGGGGTCGTCGCCCTGGTGACCTGGTTCGCGCGG
CTTAACTAACAACAACGCCC

Downstream 100 bases:

>100_bases
GCGCGACGCGATGAACATGCCCCTGAGCCTGATGCAATTTGCCCCCGCGCTGCCCGAGATTTTCGTGCTGGCGATGGTTT
CGCTGATCCTCGTGATCGAT

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MFGQSILSLVIWVPILAGAAVLATGSDRNAPLARWLALLGAVLGLAVAIPLVTGFDNATSAMQFVEKKSWIPAFNVFYHL
GVDGISMPLILLNSFVTVLVVIAGWQVIQDKVAQYMAAFLIMSGLINGAFAALDGILFYVFFEGMLIPLYLIVGVWGGPN
RVYAAFKFFLYTLLGSLLMLVSLIYLYFQSGGSFDIQSWYTTPLSMTAQTLMFFAFLLAFGVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAITLKVGAYGLVRFLLPIVPDAAHEYAWFVITLSLIAVAYIGLVALVQADMKKLIAYSSIAHMGFVTLGFF
LFNPLGWEGGLVQMISHGFVSAALFLCIGVMYDRLHSRQIKDYGGLVNRMPVFAAFFMLFAMANSGLPATSGFVGEFMVI
MAATEVNFWFAFVAATTLIFGAAYTLWMYKRVVFGSVTNSRVAQMRDVNAREILLLGLLALFVLGMGLYPKPFTDVMHAS
VVDLLAHAAQSKLP

Sequences:

>Translated_494_residues
MFGQSILSLVIWVPILAGAAVLATGSDRNAPLARWLALLGAVLGLAVAIPLVTGFDNATSAMQFVEKKSWIPAFNVFYHL
GVDGISMPLILLNSFVTVLVVIAGWQVIQDKVAQYMAAFLIMSGLINGAFAALDGILFYVFFEGMLIPLYLIVGVWGGPN
RVYAAFKFFLYTLLGSLLMLVSLIYLYFQSGGSFDIQSWYTTPLSMTAQTLMFFAFLLAFGVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAITLKVGAYGLVRFLLPIVPDAAHEYAWFVITLSLIAVAYIGLVALVQADMKKLIAYSSIAHMGFVTLGFF
LFNPLGWEGGLVQMISHGFVSAALFLCIGVMYDRLHSRQIKDYGGLVNRMPVFAAFFMLFAMANSGLPATSGFVGEFMVI
MAATEVNFWFAFVAATTLIFGAAYTLWMYKRVVFGSVTNSRVAQMRDVNAREILLLGLLALFVLGMGLYPKPFTDVMHAS
VVDLLAHAAQSKLP
>Mature_494_residues
MFGQSILSLVIWVPILAGAAVLATGSDRNAPLARWLALLGAVLGLAVAIPLVTGFDNATSAMQFVEKKSWIPAFNVFYHL
GVDGISMPLILLNSFVTVLVVIAGWQVIQDKVAQYMAAFLIMSGLINGAFAALDGILFYVFFEGMLIPLYLIVGVWGGPN
RVYAAFKFFLYTLLGSLLMLVSLIYLYFQSGGSFDIQSWYTTPLSMTAQTLMFFAFLLAFGVKVPMWPVHTWLPDAHVEA
PTGGSVVLAAITLKVGAYGLVRFLLPIVPDAAHEYAWFVITLSLIAVAYIGLVALVQADMKKLIAYSSIAHMGFVTLGFF
LFNPLGWEGGLVQMISHGFVSAALFLCIGVMYDRLHSRQIKDYGGLVNRMPVFAAFFMLFAMANSGLPATSGFVGEFMVI
MAATEVNFWFAFVAATTLIFGAAYTLWMYKRVVFGSVTNSRVAQMRDVNAREILLLGLLALFVLGMGLYPKPFTDVMHAS
VVDLLAHAAQSKLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=328, Percent_Identity=36.890243902439, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI251831117, Length=233, Percent_Identity=26.6094420600858, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788613, Length=497, Percent_Identity=34.8088531187123, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1788827, Length=273, Percent_Identity=28.9377289377289, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788831, Length=282, Percent_Identity=28.0141843971631, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI145693160, Length=457, Percent_Identity=25.601750547046, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788829, Length=409, Percent_Identity=25.9168704156479, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788614, Length=424, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367154, Length=263, Percent_Identity=26.9961977186312, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53837; Mature: 53837

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCC
>Mature Secondary Structure
MFGQSILSLVIWVPILAGAAVLATGSDRNAPLARWLALLGAVLGLAVAIPLVTGFDNATS
CCHHHHHHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AMQFVEKKSWIPAFNVFYHLGVDGISMPLILLNSFVTVLVVIAGWQVIQDKVAQYMAAFL
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMSGLINGAFAALDGILFYVFFEGMLIPLYLIVGVWGGPNRVYAAFKFFLYTLLGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VSLIYLYFQSGGSFDIQSWYTTPLSMTAQTLMFFAFLLAFGVKVPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
PTGGSVVLAAITLKVGAYGLVRFLLPIVPDAAHEYAWFVITLSLIAVAYIGLVALVQADM
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLIAYSSIAHMGFVTLGFFLFNPLGWEGGLVQMISHGFVSAALFLCIGVMYDRLHSRQI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDYGGLVNRMPVFAAFFMLFAMANSGLPATSGFVGEFMVIMAATEVNFWFAFVAATTLIF
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAYTLWMYKRVVFGSVTNSRVAQMRDVNAREILLLGLLALFVLGMGLYPKPFTDVMHAS
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VVDLLAHAAQSKLP
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]