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The map label for this gene is proB

Identifier: 74317138

GI number: 74317138

Start: 1175856

End: 1176980

Strand: Direct

Name: proB

Synonym: Tbd_1120

Alternate gene names: 74317138

Gene position: 1175856-1176980 (Clockwise)

Preceding gene: 74317137

Following gene: 74317139

Centisome position: 40.41

GC content: 70.04

Gene sequence:

>1125_bases
GTGAGCACGTCGGTCGTCCGCCACGCACGGCGCATCGTCGTCAAGGTCGGTTCCAGCCTGGTCACGGCGGACGGGCGCGG
CCTCGACCACGCGGCGCTGTCGCGCTGGGCCGAGCAGATCGCCGCGCTGGCCGAACAGGGCAAGGAAGTCGTGCTCGTGT
CGTCGGGCGCGATCGCCGAGGGCATCGCGCGCCTCGGCTGGAAAAAGCGCCCGAAAGCGGTCAACGAGTTGCAGGCCGCG
GCGGCGGTCGGGCAGATGGGTCTCGTGCAGGCCTACGAGTCGATTTTTCGCACCCACAAGTTGCATGCGGCCCAAGTTCT
TCTGACCCACGAGGATCTCGCCGACCGCACGCGCTATCTCAACGCGCGCTCGACGCTACGCACGCTGCTTGAATTACGCG
TGGTCCCGATCATCAACGAGAACGACACGGTGGCGACCGACGAGATCCGTCTCGGCGACAACGATACGCTGGGCGCGCTC
GTGACCAATCTGATCGAAGCCGACTGCCTCGTCATCCTCACCGACCAGGCGGGGCTTTATACGGCCGATCCGCGCAAGGT
CGCCGATGCGAGCCTCGTCATGGAGGCGCACGCCGGCGACCCCGAGCTCGAGCGCATGGCCGGCGGGGCCGGTAGCAGCG
TCGGGACCGGCGGCATGCTGACCAAGATCCTCGCCGCCAAGCGCGCGGCGCGCAGCGGCGCGCACACCGTGATCTGCAGC
GGCCGCGAGCCGCGCGTGCTGCTGCGTCTTGCCGAGGGCGAACCCATCGGCAGCCAGCTGGTCGCGCGCCAGGCGCCCCT
CGCCGCGCGCCGCCAGTGGCTCGCCGATCACCTGCAACTGCGCGGCGGGCTCGTGCTCGACGACGGCGCCGTGCGCGCGC
TGCAGGAGGACGGCAAGAGCCTCCTGCCGGTCGGCGTCCGGGGCGTGACCGGAGAATTCGAACGCGGCGAGGTCGTCGCC
GTCGTCGACGCGAGCGGACGCGAGATCGCCCGCGGCCTGACCAACTACAGCGCCGCCGAAGCGCGCCGTATCGCGGGCAA
GCCCAGTACCGCGATCGAAGCCGAGCTCGGCTACATGGACGAGCCCGAACTGATCCACCGCGACAACCTGGTCGTTCTGG
CGTGA

Upstream 100 bases:

>100_bases
TGTCCTACCATAAGGCCTTGTTCGAGGATCATTTCTGTCCCGACGGCATCAGCGTTCGCGATGCCAAGCAAGCCGTGCAG
AGCCTGCGTCGGGCGTCGCG

Downstream 100 bases:

>100_bases
CGACCGTGCTGGACTTCCTGCGGCACGGCGAACCCGTCGGCGGCCGCAAGTACCGCGGGCAGGTCGACGACGCGCTGTCC
GAGTTGGGCTGGGCCCAGAT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSTSVVRHARRIVVKVGSSLVTADGRGLDHAALSRWAEQIAALAEQGKEVVLVSSGAIAEGIARLGWKKRPKAVNELQAA
AAVGQMGLVQAYESIFRTHKLHAAQVLLTHEDLADRTRYLNARSTLRTLLELRVVPIINENDTVATDEIRLGDNDTLGAL
VTNLIEADCLVILTDQAGLYTADPRKVADASLVMEAHAGDPELERMAGGAGSSVGTGGMLTKILAAKRAARSGAHTVICS
GREPRVLLRLAEGEPIGSQLVARQAPLAARRQWLADHLQLRGGLVLDDGAVRALQEDGKSLLPVGVRGVTGEFERGEVVA
VVDASGREIARGLTNYSAAEARRIAGKPSTAIEAELGYMDEPELIHRDNLVVLA

Sequences:

>Translated_374_residues
MSTSVVRHARRIVVKVGSSLVTADGRGLDHAALSRWAEQIAALAEQGKEVVLVSSGAIAEGIARLGWKKRPKAVNELQAA
AAVGQMGLVQAYESIFRTHKLHAAQVLLTHEDLADRTRYLNARSTLRTLLELRVVPIINENDTVATDEIRLGDNDTLGAL
VTNLIEADCLVILTDQAGLYTADPRKVADASLVMEAHAGDPELERMAGGAGSSVGTGGMLTKILAAKRAARSGAHTVICS
GREPRVLLRLAEGEPIGSQLVARQAPLAARRQWLADHLQLRGGLVLDDGAVRALQEDGKSLLPVGVRGVTGEFERGEVVA
VVDASGREIARGLTNYSAAEARRIAGKPSTAIEAELGYMDEPELIHRDNLVVLA
>Mature_373_residues
STSVVRHARRIVVKVGSSLVTADGRGLDHAALSRWAEQIAALAEQGKEVVLVSSGAIAEGIARLGWKKRPKAVNELQAAA
AVGQMGLVQAYESIFRTHKLHAAQVLLTHEDLADRTRYLNARSTLRTLLELRVVPIINENDTVATDEIRLGDNDTLGALV
TNLIEADCLVILTDQAGLYTADPRKVADASLVMEAHAGDPELERMAGGAGSSVGTGGMLTKILAAKRAARSGAHTVICSG
REPRVLLRLAEGEPIGSQLVARQAPLAARRQWLADHLQLRGGLVLDDGAVRALQEDGKSLLPVGVRGVTGEFERGEVVAV
VDASGREIARGLTNYSAAEARRIAGKPSTAIEAELGYMDEPELIHRDNLVVLA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=34.8275862068965, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=35.0694444444444, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=44.0771349862259, Blast_Score=294, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=33.8235294117647, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=33.8235294117647, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=35.3365384615385, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=415, Percent_Identity=33.0120481927711, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=33.7979094076655, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_THIDA (Q3SJS7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314878.1
- ProteinModelPortal:   Q3SJS7
- SMR:   Q3SJS7
- STRING:   Q3SJS7
- GeneID:   3673191
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1120
- NMPDR:   fig|292415.3.peg.1120
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q3SJS7
- ProtClustDB:   PRK05429
- BioCyc:   TDEN292415:TBD_1120-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39769; Mature: 39638

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVVRHARRIVVKVGSSLVTADGRGLDHAALSRWAEQIAALAEQGKEVVLVSSGAIAE
CCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHH
GIARLGWKKRPKAVNELQAAAAVGQMGLVQAYESIFRTHKLHAAQVLLTHEDLADRTRYL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHH
NARSTLRTLLELRVVPIINENDTVATDEIRLGDNDTLGALVTNLIEADCLVILTDQAGLY
HHHHHHHHHHHHHEEEEECCCCCEEECEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCE
TADPRKVADASLVMEAHAGDPELERMAGGAGSSVGTGGMLTKILAAKRAARSGAHTVICS
ECCCHHHHHHHHEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEC
GREPRVLLRLAEGEPIGSQLVARQAPLAARRQWLADHLQLRGGLVLDDGAVRALQEDGKS
CCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCC
LLPVGVRGVTGEFERGEVVAVVDASGREIARGLTNYSAAEARRIAGKPSTAIEAELGYMD
CCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCCCC
EPELIHRDNLVVLA
CCCEEECCCEEEEC
>Mature Secondary Structure 
STSVVRHARRIVVKVGSSLVTADGRGLDHAALSRWAEQIAALAEQGKEVVLVSSGAIAE
CHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHH
GIARLGWKKRPKAVNELQAAAAVGQMGLVQAYESIFRTHKLHAAQVLLTHEDLADRTRYL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHH
NARSTLRTLLELRVVPIINENDTVATDEIRLGDNDTLGALVTNLIEADCLVILTDQAGLY
HHHHHHHHHHHHHEEEEECCCCCEEECEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCE
TADPRKVADASLVMEAHAGDPELERMAGGAGSSVGTGGMLTKILAAKRAARSGAHTVICS
ECCCHHHHHHHHEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEC
GREPRVLLRLAEGEPIGSQLVARQAPLAARRQWLADHLQLRGGLVLDDGAVRALQEDGKS
CCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCC
LLPVGVRGVTGEFERGEVVAVVDASGREIARGLTNYSAAEARRIAGKPSTAIEAELGYMD
CCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCCCC
EPELIHRDNLVVLA
CCCEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA