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The map label for this gene is yfgF [C]

Identifier: 74317079

GI number: 74317079

Start: 1123136

End: 1124500

Strand: Reverse

Name: yfgF [C]

Synonym: Tbd_1061

Alternate gene names: 74317079

Gene position: 1124500-1123136 (Counterclockwise)

Preceding gene: 74317081

Following gene: 74317078

Centisome position: 38.65

GC content: 65.35

Gene sequence:

>1365_bases
ATGACAGGCGTGTTTCACGCGTTCACAAGCGCAAAAAAAATGGCGCGCGCCGGTCGTCGAACGTTGCGCTTGATGGTGGC
GCGACCTAGTGTGCAGGGTGCTTCCCCCACGCACGCGACACGTATCACCCTCCGGACGGAACCGAGTATGCAAACACGCG
AAGACAAAATGAGCATACTGGACGTCGACGTCTGCGGCACGCCCCAGCAGATCGACGAGATCGTTTCCGACGCCCTGCGT
TCGGTCCGCAGCCTGCTCGGCATGCAGGTCGCGTTCGTGTCAGAGTTTCGGGACGGCGCGCGCATCTTTCGCCACGTCGA
TGCGGACGCCGACTTTTCGGTCATTCAGGTCGGTGCATGGGACCCGCTCGACGCGTCGTATTGCCAGCGTGTCGTCGACG
GACGCCTGCCTCCCTTGATGCGCGACGCCTGCCAGGAGCCGGCCGCCCTGGCCCTGCCGGTGACGACCGAATTACCGGTG
GGCGCCCATCTCAGCGTGCCGATCCGGTTCCGGGACGGCCGCGTCTACGGGACCTTCTGCTGCTTCAGCACCTTCGCCGA
CGCGTCGCTCACCGAGCGCGACCTCGGGACCTTGCGCCTGTTCGCGGATTTCGTCGCCAAGCTGCTCGAAAGGAAGTCGA
ACGAAACCACCCGCGAAAACATCATCCGCGAGCGCGTGCGCGCCGTGCTCGACGGCCGGCATTACCGTATCGTCTACCAG
CCCATCTTCAACCTCGAACCGAATGCGCTCGTCGGATACGAGGCCCTGACGCGGTTCTCGCTAGAACCCGTCAGGACGCC
CGATGCGTGGTTTTGCGAAGCGGACGAGGTCGGCCTCGACGAGGAGCTCGAACTGGCGGCGATTCGCGCGGCCCTGGCGG
TCGTCGCGCGCCTGCCGACGACGGTTTATCTGTCGCTCAATGTCTCGCCGAACACGATTCTGAAGGGCTCGCTGGCCTCG
GCCTTGAACGGCCATCCCCTCGAACGCCTGATGCTGGAAGTGACCGAACACGCGTCGATCAACGACTACGAGCGGGTGGC
CGCGACACTCGCGCCGCTACGGCAGCAGGGCTTGCGACTCGCGGTCGACGACGCCGGCGCCGGCTTCGCGAGCTTTCGCC
ACATCCTGCAGTTGAAGCCCGACGTGATCAAACTCGACTACAGCCTGATCCACGGCATCGATCGCGACGGCGACCGTCGC
GCCCTCGCCGCAGCGGTCATCCGCTTCGCCGAGGCGACCGGCAGCAAGGTCGTCGCCGAGGGCGTCGAGACCGAAGGCGA
ATTGCAGGCGCTGCGCGAGCTCAACGTGAACAAGGTGCAGGGTTACTTGCTTGGGCGTCCGGCGCCCTATCCCGGCGACG
CCTAG

Upstream 100 bases:

>100_bases
GGTGCCTCGTGCAAAGCGCCGCCGTCGCATTCATTTCAAATTATTTTGAGCGCGCCGGGCGTGGAATGTTTCCTTGTGCC
TTCGCTTTCACGGGCGGGCC

Downstream 100 bases:

>100_bases
GGATTCTGGCGGCGCCTGCTGTCTGGTTTAATGTCGCGTCCTCCATATCCGGCATGCGAAGCCGGTCGCCCCGCTTGACG
CGGCGCGGATCGCGCCGCTC

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTGVFHAFTSAKKMARAGRRTLRLMVARPSVQGASPTHATRITLRTEPSMQTREDKMSILDVDVCGTPQQIDEIVSDALR
SVRSLLGMQVAFVSEFRDGARIFRHVDADADFSVIQVGAWDPLDASYCQRVVDGRLPPLMRDACQEPAALALPVTTELPV
GAHLSVPIRFRDGRVYGTFCCFSTFADASLTERDLGTLRLFADFVAKLLERKSNETTRENIIRERVRAVLDGRHYRIVYQ
PIFNLEPNALVGYEALTRFSLEPVRTPDAWFCEADEVGLDEELELAAIRAALAVVARLPTTVYLSLNVSPNTILKGSLAS
ALNGHPLERLMLEVTEHASINDYERVAATLAPLRQQGLRLAVDDAGAGFASFRHILQLKPDVIKLDYSLIHGIDRDGDRR
ALAAAVIRFAEATGSKVVAEGVETEGELQALRELNVNKVQGYLLGRPAPYPGDA

Sequences:

>Translated_454_residues
MTGVFHAFTSAKKMARAGRRTLRLMVARPSVQGASPTHATRITLRTEPSMQTREDKMSILDVDVCGTPQQIDEIVSDALR
SVRSLLGMQVAFVSEFRDGARIFRHVDADADFSVIQVGAWDPLDASYCQRVVDGRLPPLMRDACQEPAALALPVTTELPV
GAHLSVPIRFRDGRVYGTFCCFSTFADASLTERDLGTLRLFADFVAKLLERKSNETTRENIIRERVRAVLDGRHYRIVYQ
PIFNLEPNALVGYEALTRFSLEPVRTPDAWFCEADEVGLDEELELAAIRAALAVVARLPTTVYLSLNVSPNTILKGSLAS
ALNGHPLERLMLEVTEHASINDYERVAATLAPLRQQGLRLAVDDAGAGFASFRHILQLKPDVIKLDYSLIHGIDRDGDRR
ALAAAVIRFAEATGSKVVAEGVETEGELQALRELNVNKVQGYLLGRPAPYPGDA
>Mature_453_residues
TGVFHAFTSAKKMARAGRRTLRLMVARPSVQGASPTHATRITLRTEPSMQTREDKMSILDVDVCGTPQQIDEIVSDALRS
VRSLLGMQVAFVSEFRDGARIFRHVDADADFSVIQVGAWDPLDASYCQRVVDGRLPPLMRDACQEPAALALPVTTELPVG
AHLSVPIRFRDGRVYGTFCCFSTFADASLTERDLGTLRLFADFVAKLLERKSNETTRENIIRERVRAVLDGRHYRIVYQP
IFNLEPNALVGYEALTRFSLEPVRTPDAWFCEADEVGLDEELELAAIRAALAVVARLPTTVYLSLNVSPNTILKGSLASA
LNGHPLERLMLEVTEHASINDYERVAATLAPLRQQGLRLAVDDAGAGFASFRHILQLKPDVIKLDYSLIHGIDRDGDRRA
LAAAVIRFAEATGSKVVAEGVETEGELQALRELNVNKVQGYLLGRPAPYPGDA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI87082096, Length=252, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788849, Length=240, Percent_Identity=27.5, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081980, Length=235, Percent_Identity=29.3617021276596, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790496, Length=245, Percent_Identity=25.7142857142857, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787055, Length=278, Percent_Identity=27.3381294964029, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081845, Length=285, Percent_Identity=29.8245614035088, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081921, Length=253, Percent_Identity=27.6679841897233, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510982, Length=243, Percent_Identity=30.0411522633745, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786507, Length=254, Percent_Identity=27.1653543307087, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788502, Length=241, Percent_Identity=29.045643153527, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081743, Length=261, Percent_Identity=26.0536398467433, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=237, Percent_Identity=26.5822784810127, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787410, Length=150, Percent_Identity=28, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT; PF00989 PAS [H]

EC number: NA

Molecular weight: Translated: 49993; Mature: 49862

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVFHAFTSAKKMARAGRRTLRLMVARPSVQGASPTHATRITLRTEPSMQTREDKMSIL
CCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHCCCEEE
DVDVCGTPQQIDEIVSDALRSVRSLLGMQVAFVSEFRDGARIFRHVDADADFSVIQVGAW
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
DPLDASYCQRVVDGRLPPLMRDACQEPAALALPVTTELPVGAHLSVPIRFRDGRVYGTFC
CCCCHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEEECCEEEEEHH
CFSTFADASLTERDLGTLRLFADFVAKLLERKSNETTRENIIRERVRAVLDGRHYRIVYQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
PIFNLEPNALVGYEALTRFSLEPVRTPDAWFCEADEVGLDEELELAAIRAALAVVARLPT
EECCCCCCCEECHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCE
TVYLSLNVSPNTILKGSLASALNGHPLERLMLEVTEHASINDYERVAATLAPLRQQGLRL
EEEEEEECCCCCEECCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEE
AVDDAGAGFASFRHILQLKPDVIKLDYSLIHGIDRDGDRRALAAAVIRFAEATGSKVVAE
EEECCCCCHHHHHHHHHCCCCCEEECHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
GVETEGELQALRELNVNKVQGYLLGRPAPYPGDA
CCCCHHHHHHHHHCCHHHHCEEEECCCCCCCCCC
>Mature Secondary Structure 
TGVFHAFTSAKKMARAGRRTLRLMVARPSVQGASPTHATRITLRTEPSMQTREDKMSIL
CCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHCCCEEE
DVDVCGTPQQIDEIVSDALRSVRSLLGMQVAFVSEFRDGARIFRHVDADADFSVIQVGAW
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
DPLDASYCQRVVDGRLPPLMRDACQEPAALALPVTTELPVGAHLSVPIRFRDGRVYGTFC
CCCCHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEEECCEEEEEHH
CFSTFADASLTERDLGTLRLFADFVAKLLERKSNETTRENIIRERVRAVLDGRHYRIVYQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
PIFNLEPNALVGYEALTRFSLEPVRTPDAWFCEADEVGLDEELELAAIRAALAVVARLPT
EECCCCCCCEECHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCE
TVYLSLNVSPNTILKGSLASALNGHPLERLMLEVTEHASINDYERVAATLAPLRQQGLRL
EEEEEEECCCCCEECCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEE
AVDDAGAGFASFRHILQLKPDVIKLDYSLIHGIDRDGDRRALAAAVIRFAEATGSKVVAE
EEECCCCCHHHHHHHHHCCCCCEEECHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
GVETEGELQALRELNVNKVQGYLLGRPAPYPGDA
CCCCHHHHHHHHHCCHHHHCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]