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The map label for this gene is rlmA [H]

Identifier: 74317077

GI number: 74317077

Start: 1121794

End: 1122642

Strand: Reverse

Name: rlmA [H]

Synonym: Tbd_1059

Alternate gene names: 74317077

Gene position: 1122642-1121794 (Counterclockwise)

Preceding gene: 74317078

Following gene: 74317076

Centisome position: 38.58

GC content: 70.67

Gene sequence:

>849_bases
ATGCCGGTAACGCTCTTCCAGGCGCTCGCCTGCCCGCTGGACGGCACGCCCCTGCAGCGCGACGGCGCCGCATGGCGCTG
CCCTTCGGGCCACAGCTTCGATGTCGCACGCCAGAACTACGCGAACCTGCTGCCGGTGCAGCACAAGCGCTCGCGTGATC
CGGGCGACAGCAAGGAGATGGTCGCGGCACGGCGGCGCTTTCTCGCTGCGGGTTTCTACGCGCCGATCGCCGATGCCGTG
AACCGGGCCGTGCTCGCTGACCTGCCGCCGGACGCGACCGTCGCCTGCCTCGACGCCGGATGCGGCGAGGGCTACTACCT
GCGTCAACTCGCGGCGTCGGCACCGGCCGGCTGCAGGCTGGCACTCATGGGCCTCGACATTTCGAAATGGGCGGTGCTGT
CGGCTGCGAAGCAGGATGCGCGGGTCAGCTGGGTCGTAGGCAGCAACGCCCACCTGCCGGTACTGCCGGGCACGCTCGAC
CGCGTGCTGTGCATGTTCGGCTTTCCGGTCTACCCCGAGTTCGCGCGCGTGCTGAAGCCGGGCGGACGGCTCATCCAGGT
CGACGCCGGGCCCGAGCATCTGCGCGAGCTGCGCGAGATCATCTACCCCGCGCTGAAGCCGCAACGCACAGCGGACCCGC
CGACCCCGGCGGGCTTCACCGAGCGACCGGCGACGACGCTGCGCTTTCGCATCGAACTCGCAAGCGCGCAGGAGATCGCC
GACCTGCTCGCGATGACGCCGCATCTCTACCGCGCCACGGCCGAAGCGCGCGCGGACGCCGCCGCGCTGACGGCGCTCTC
GCTCGGCGTCGACGTGCGGCTCACCTGTTTCGAGCGGGAGGCAGTCTAA

Upstream 100 bases:

>100_bases
CCATATCGGCAAGCTGCTGGAACTGTTTCCCCTCCACGCCGTGCTGAGCTATCCGGTGGATGCGCGTCGGCTCGAGGCGG
TTCTCGCGCAGCCATAAGCG

Downstream 100 bases:

>100_bases
GAGAGGTACCCATGGTGCTTCATTGGGGATTTTGTTCTAAGATATTGCCCTCTGAATATCCGCACTAAGGGCCGATCATG
AAACGCTGGAACGTCAAATA

Product: rRNA (guanine-N1-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPVTLFQALACPLDGTPLQRDGAAWRCPSGHSFDVARQNYANLLPVQHKRSRDPGDSKEMVAARRRFLAAGFYAPIADAV
NRAVLADLPPDATVACLDAGCGEGYYLRQLAASAPAGCRLALMGLDISKWAVLSAAKQDARVSWVVGSNAHLPVLPGTLD
RVLCMFGFPVYPEFARVLKPGGRLIQVDAGPEHLRELREIIYPALKPQRTADPPTPAGFTERPATTLRFRIELASAQEIA
DLLAMTPHLYRATAEARADAAALTALSLGVDVRLTCFEREAV

Sequences:

>Translated_282_residues
MPVTLFQALACPLDGTPLQRDGAAWRCPSGHSFDVARQNYANLLPVQHKRSRDPGDSKEMVAARRRFLAAGFYAPIADAV
NRAVLADLPPDATVACLDAGCGEGYYLRQLAASAPAGCRLALMGLDISKWAVLSAAKQDARVSWVVGSNAHLPVLPGTLD
RVLCMFGFPVYPEFARVLKPGGRLIQVDAGPEHLRELREIIYPALKPQRTADPPTPAGFTERPATTLRFRIELASAQEIA
DLLAMTPHLYRATAEARADAAALTALSLGVDVRLTCFEREAV
>Mature_281_residues
PVTLFQALACPLDGTPLQRDGAAWRCPSGHSFDVARQNYANLLPVQHKRSRDPGDSKEMVAARRRFLAAGFYAPIADAVN
RAVLADLPPDATVACLDAGCGEGYYLRQLAASAPAGCRLALMGLDISKWAVLSAAKQDARVSWVVGSNAHLPVLPGTLDR
VLCMFGFPVYPEFARVLKPGGRLIQVDAGPEHLRELREIIYPALKPQRTADPPTPAGFTERPATTLRFRIELASAQEIAD
LLAMTPHLYRATAEARADAAALTALSLGVDVRLTCFEREAV

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=272, Percent_Identity=39.3382352941176, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 30440; Mature: 30309

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTLFQALACPLDGTPLQRDGAAWRCPSGHSFDVARQNYANLLPVQHKRSRDPGDSKEM
CCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCHHCCCCCCCHHHH
VAARRRFLAAGFYAPIADAVNRAVLADLPPDATVACLDAGCGEGYYLRQLAASAPAGCRL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEE
ALMGLDISKWAVLSAAKQDARVSWVVGSNAHLPVLPGTLDRVLCMFGFPVYPEFARVLKP
EEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCC
GGRLIQVDAGPEHLRELREIIYPALKPQRTADPPTPAGFTERPATTLRFRIELASAQEIA
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHH
DLLAMTPHLYRATAEARADAAALTALSLGVDVRLTCFEREAV
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure 
PVTLFQALACPLDGTPLQRDGAAWRCPSGHSFDVARQNYANLLPVQHKRSRDPGDSKEM
CHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCHHCCCCCCCHHHH
VAARRRFLAAGFYAPIADAVNRAVLADLPPDATVACLDAGCGEGYYLRQLAASAPAGCRL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEE
ALMGLDISKWAVLSAAKQDARVSWVVGSNAHLPVLPGTLDRVLCMFGFPVYPEFARVLKP
EEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCC
GGRLIQVDAGPEHLRELREIIYPALKPQRTADPPTPAGFTERPATTLRFRIELASAQEIA
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHH
DLLAMTPHLYRATAEARADAAALTALSLGVDVRLTCFEREAV
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]