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The map label for this gene is thrS

Identifier: 74317025

GI number: 74317025

Start: 1075850

End: 1077763

Strand: Direct

Name: thrS

Synonym: Tbd_1007

Alternate gene names: 74317025

Gene position: 1075850-1077763 (Clockwise)

Preceding gene: 74317024

Following gene: 74317026

Centisome position: 36.97

GC content: 64.63

Gene sequence:

>1914_bases
ATGGTCACTGTCACGCTGCCCGACGGCTCGGTCCGCCCCTTCGAGGGTCCCGTCACGGTCGCCGAGGTCGCGTCGAGCAT
CGGCGCCGGGCTTGCCAAGGCGGCACTCGCGGGCAAGGTCGACGGCAAACTCGTCGATACCAGCTACGTGATCGACGCCG
ACGCGCAGCTCGCGATCGTCACCGCCAAGGACGCCGAGGCGCTCGACCTGATCCGCCACGACGCGGCGCACGTGATGGCG
CAGGCCGTGCAGGAGCTCTACCCGGGTACCCAGGTGACGATCGGCCCGGCGATCGAGGACGGCTTCTACTACGACTTCGC
GCGCGAGCAGCCTTTCACGCCAGAAGACCTCGAGAAGATCGAGAAGCGCATGGACGAGATCGTCAAGCGCGACCTGCCGA
TCCGGCGCGAGGTGTGGAGCCGCGACGAGGCGATGAAGGTGTTCGGCGATCTCGGCGAGACCTACAAGGTGCAGATCATC
GACGAGGTGATACCCAAGGGCGAGGAGCTGTCGATCTACCGTCAGGGCGAGTGGTTCGACGTCTGCCGCGGCCCCCACCT
GCCGTCGACCGGCAAGTTGCCGCGCGCGTTCAAGCTGATGAAGCTCGCCGGCGCGTACTGGCGCGGCGACTCGAAGAACG
CGATGCTGCAGCGCATTTACGGCACGGCGTGGGCGAAGAAGGAGGACCTCGAGGCCTATCTGCACCGGCTGGAGGAGGCC
GAGAAGCGCGACCACCGCCGGCTCGCCAAGCAGCTCGACCTCCTGCACATGCAGGATGAAGCGCCGGGCATGGTGTTCTG
GCACCCCAAGGGCTGGATCGTCTGGCAGCAGATCGAGCAGTACATGCGCGAGAAATTCGTCGAATACGGCTATCAGGAAG
TGCGCACGCCGGCTGTCATGGACCGCAGCATGTGGGAGAAATCCGGCCACTGGGAAAACTACCGCGACAACATGTTCACG
ACGGCGTCGGAAAACCGCGACTACGCGGTCAAGCCGATGAACTGCCCAGGTCACGTGCAGATCTTCAATAGCGGGCTGCA
TTCCTATCGGGACCTGCCGCTCAGGCTCGCCGAGTTCGGCTCGTGCCACCGCAACGAGCCGTCGGGCGCGCTGCACGGCA
TCATGCGCGTGCGCGGCTTCACTCAGGACGACGCGCACATCTTCTGCATGGAGGAGCAGGTCGAGCAGGAGGTGGCCGAC
TTCATCGTCATGCTGCAGAAGGTCTACGCCGACTTTGGCTTCAACGACGTATTGGTCAAGCTTTCGACCCGGCCCGACAA
GCGCGTCGGCTCGGACGAGAGCTGGGATAAGGCCGAGTCGGCGCTCGCAGCCGCGCTCGAGAAGAACGGTCTCTCCTTCG
ACCTGCAGCCGGGTGAGGGCGCGTTCTACGGGCCGAAGATCGAATTCACGCTCAAGGACACGCTCGGCCGTCTGTGGCAG
TGCGGCACGATCCAACTCGACTTCAACCTGCCGGTGCGGCTCGGCGCCGAATTCGTCGCCGAGGACAACACGCGCAAGAT
CCCGGTGATGCTCCACCGCGCGATCCTCGGCTCGATGGAGCGCTTCATCGGCATCCTGATCGAACACCACGCCGGGAACT
TCCCGTTGTGGCTCGCGCCGGTCCAGGTCATGGTGATGAACATCAGCGAGCGCCAGGCGGCCTACGCCGAGGCGGTCGCC
GAGGCCTTGCGCCGCGCCGGAATCCGGGCCGCGCTGGACTTGAGTAATAACAAGATTAACTATAAAATCCGCGAGCACAG
CTTGCAGAAGCTGCCCTATCAGCTTGTCGTCGGCGACAAGGAAATGGAGGCGCGCGTGGTCGCAGTCCGCGCCCGCGGCA
ACCAGGACATGGGGCAGTTGGGTTTGGATGACTTGATTGCGCGGCTCAGGGCGGAAGTCCTGGCGCGTCAGTAA

Upstream 100 bases:

>100_bases
CGGCGCACAGTCGTTCGCAGACGTTCTGCAATCTTGAAAAATGTAGTACCCCGAAAAGCGCGGCCGGTCCGCGCTTTTTT
TCTTTTCCGGAGTCTGAAAC

Downstream 100 bases:

>100_bases
CAGCGGTCAAACAAGGCGTGGGGACTTCGTTCCGCGCCTTCTTTATTTAGTGTTTGGCCATTTGCCTCGGCATGGCGCCG
GGTTTTATTTGGAGAACGAC

Product: Ser-tRNA(Thr) hydrolase / threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MVTVTLPDGSVRPFEGPVTVAEVASSIGAGLAKAALAGKVDGKLVDTSYVIDADAQLAIVTAKDAEALDLIRHDAAHVMA
QAVQELYPGTQVTIGPAIEDGFYYDFAREQPFTPEDLEKIEKRMDEIVKRDLPIRREVWSRDEAMKVFGDLGETYKVQII
DEVIPKGEELSIYRQGEWFDVCRGPHLPSTGKLPRAFKLMKLAGAYWRGDSKNAMLQRIYGTAWAKKEDLEAYLHRLEEA
EKRDHRRLAKQLDLLHMQDEAPGMVFWHPKGWIVWQQIEQYMREKFVEYGYQEVRTPAVMDRSMWEKSGHWENYRDNMFT
TASENRDYAVKPMNCPGHVQIFNSGLHSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRGFTQDDAHIFCMEEQVEQEVAD
FIVMLQKVYADFGFNDVLVKLSTRPDKRVGSDESWDKAESALAAALEKNGLSFDLQPGEGAFYGPKIEFTLKDTLGRLWQ
CGTIQLDFNLPVRLGAEFVAEDNTRKIPVMLHRAILGSMERFIGILIEHHAGNFPLWLAPVQVMVMNISERQAAYAEAVA
EALRRAGIRAALDLSNNKINYKIREHSLQKLPYQLVVGDKEMEARVVAVRARGNQDMGQLGLDDLIARLRAEVLARQ

Sequences:

>Translated_637_residues
MVTVTLPDGSVRPFEGPVTVAEVASSIGAGLAKAALAGKVDGKLVDTSYVIDADAQLAIVTAKDAEALDLIRHDAAHVMA
QAVQELYPGTQVTIGPAIEDGFYYDFAREQPFTPEDLEKIEKRMDEIVKRDLPIRREVWSRDEAMKVFGDLGETYKVQII
DEVIPKGEELSIYRQGEWFDVCRGPHLPSTGKLPRAFKLMKLAGAYWRGDSKNAMLQRIYGTAWAKKEDLEAYLHRLEEA
EKRDHRRLAKQLDLLHMQDEAPGMVFWHPKGWIVWQQIEQYMREKFVEYGYQEVRTPAVMDRSMWEKSGHWENYRDNMFT
TASENRDYAVKPMNCPGHVQIFNSGLHSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRGFTQDDAHIFCMEEQVEQEVAD
FIVMLQKVYADFGFNDVLVKLSTRPDKRVGSDESWDKAESALAAALEKNGLSFDLQPGEGAFYGPKIEFTLKDTLGRLWQ
CGTIQLDFNLPVRLGAEFVAEDNTRKIPVMLHRAILGSMERFIGILIEHHAGNFPLWLAPVQVMVMNISERQAAYAEAVA
EALRRAGIRAALDLSNNKINYKIREHSLQKLPYQLVVGDKEMEARVVAVRARGNQDMGQLGLDDLIARLRAEVLARQ
>Mature_637_residues
MVTVTLPDGSVRPFEGPVTVAEVASSIGAGLAKAALAGKVDGKLVDTSYVIDADAQLAIVTAKDAEALDLIRHDAAHVMA
QAVQELYPGTQVTIGPAIEDGFYYDFAREQPFTPEDLEKIEKRMDEIVKRDLPIRREVWSRDEAMKVFGDLGETYKVQII
DEVIPKGEELSIYRQGEWFDVCRGPHLPSTGKLPRAFKLMKLAGAYWRGDSKNAMLQRIYGTAWAKKEDLEAYLHRLEEA
EKRDHRRLAKQLDLLHMQDEAPGMVFWHPKGWIVWQQIEQYMREKFVEYGYQEVRTPAVMDRSMWEKSGHWENYRDNMFT
TASENRDYAVKPMNCPGHVQIFNSGLHSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRGFTQDDAHIFCMEEQVEQEVAD
FIVMLQKVYADFGFNDVLVKLSTRPDKRVGSDESWDKAESALAAALEKNGLSFDLQPGEGAFYGPKIEFTLKDTLGRLWQ
CGTIQLDFNLPVRLGAEFVAEDNTRKIPVMLHRAILGSMERFIGILIEHHAGNFPLWLAPVQVMVMNISERQAAYAEAVA
EALRRAGIRAALDLSNNKINYKIREHSLQKLPYQLVVGDKEMEARVVAVRARGNQDMGQLGLDDLIARLRAEVLARQ

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=636, Percent_Identity=38.5220125786164, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI38202255, Length=633, Percent_Identity=39.1785150078989, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI20070344, Length=649, Percent_Identity=36.3636363636364, Blast_Score=424, Evalue=1e-118,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=58.8050314465409, Blast_Score=817, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=157, Percent_Identity=27.3885350318471, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71984184, Length=637, Percent_Identity=39.2464678178964, Blast_Score=476, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71984192, Length=637, Percent_Identity=39.2464678178964, Blast_Score=476, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322112, Length=630, Percent_Identity=39.0476190476191, Blast_Score=479, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322654, Length=448, Percent_Identity=36.1607142857143, Blast_Score=299, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24583842, Length=642, Percent_Identity=39.8753894080997, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI28574141, Length=642, Percent_Identity=39.8753894080997, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24583839, Length=642, Percent_Identity=39.8753894080997, Blast_Score=491, Evalue=1e-139,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_THIDA (Q3SK34)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314765.1
- ProteinModelPortal:   Q3SK34
- SMR:   Q3SK34
- STRING:   Q3SK34
- GeneID:   3671231
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1007
- NMPDR:   fig|292415.3.peg.737
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- PhylomeDB:   Q3SK34
- BioCyc:   TDEN292415:TBD_1007-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 72181; Mature: 72181

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVTLPDGSVRPFEGPVTVAEVASSIGAGLAKAALAGKVDGKLVDTSYVIDADAQLAIV
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCEEEEE
TAKDAEALDLIRHDAAHVMAQAVQELYPGTQVTIGPAIEDGFYYDFAREQPFTPEDLEKI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCCHHHHHHH
EKRMDEIVKRDLPIRREVWSRDEAMKVFGDLGETYKVQIIDEVIPKGEELSIYRQGEWFD
HHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCEEEEEEHHHHCCCCCCEEEEECCCCHH
VCRGPHLPSTGKLPRAFKLMKLAGAYWRGDSKNAMLQRIYGTAWAKKEDLEAYLHRLEEA
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EKRDHRRLAKQLDLLHMQDEAPGMVFWHPKGWIVWQQIEQYMREKFVEYGYQEVRTPAVM
HHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHCHHHHCCHHHH
DRSMWEKSGHWENYRDNMFTTASENRDYAVKPMNCPGHVQIFNSGLHSYRDLPLRLAEFG
HHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEHHHHHHHHHCCCHHHHHHC
SCHRNEPSGALHGIMRVRGFTQDDAHIFCMEEQVEQEVADFIVMLQKVYADFGFNDVLVK
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LSTRPDKRVGSDESWDKAESALAAALEKNGLSFDLQPGEGAFYGPKIEFTLKDTLGRLWQ
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEECCEEEEEEHHHHHHHHC
CGTIQLDFNLPVRLGAEFVAEDNTRKIPVMLHRAILGSMERFIGILIEHHAGNFPLWLAP
CCEEEEECCCCHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHH
VQVMVMNISERQAAYAEAVAEALRRAGIRAALDLSNNKINYKIREHSLQKLPYQLVVGDK
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHHHHCCEEEEECCC
EMEARVVAVRARGNQDMGQLGLDDLIARLRAEVLARQ
CCCEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTVTLPDGSVRPFEGPVTVAEVASSIGAGLAKAALAGKVDGKLVDTSYVIDADAQLAIV
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCEEEEE
TAKDAEALDLIRHDAAHVMAQAVQELYPGTQVTIGPAIEDGFYYDFAREQPFTPEDLEKI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCCHHHHHHH
EKRMDEIVKRDLPIRREVWSRDEAMKVFGDLGETYKVQIIDEVIPKGEELSIYRQGEWFD
HHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCEEEEEEHHHHCCCCCCEEEEECCCCHH
VCRGPHLPSTGKLPRAFKLMKLAGAYWRGDSKNAMLQRIYGTAWAKKEDLEAYLHRLEEA
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EKRDHRRLAKQLDLLHMQDEAPGMVFWHPKGWIVWQQIEQYMREKFVEYGYQEVRTPAVM
HHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHCHHHHCCHHHH
DRSMWEKSGHWENYRDNMFTTASENRDYAVKPMNCPGHVQIFNSGLHSYRDLPLRLAEFG
HHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEHHHHHHHHHCCCHHHHHHC
SCHRNEPSGALHGIMRVRGFTQDDAHIFCMEEQVEQEVADFIVMLQKVYADFGFNDVLVK
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LSTRPDKRVGSDESWDKAESALAAALEKNGLSFDLQPGEGAFYGPKIEFTLKDTLGRLWQ
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEECCEEEEEEHHHHHHHHC
CGTIQLDFNLPVRLGAEFVAEDNTRKIPVMLHRAILGSMERFIGILIEHHAGNFPLWLAP
CCEEEEECCCCHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHH
VQVMVMNISERQAAYAEAVAEALRRAGIRAALDLSNNKINYKIREHSLQKLPYQLVVGDK
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHHHHHCCEEEEECCC
EMEARVVAVRARGNQDMGQLGLDDLIARLRAEVLARQ
CCCEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA