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The map label for this gene is rumA

Identifier: 74316988

GI number: 74316988

Start: 1033581

End: 1034879

Strand: Reverse

Name: rumA

Synonym: Tbd_0970

Alternate gene names: 74316988

Gene position: 1034879-1033581 (Counterclockwise)

Preceding gene: 74316989

Following gene: 74316987

Centisome position: 35.57

GC content: 69.05

Gene sequence:

>1299_bases
ATGAATCCGGTCGTCGACATCCTTTCCCTCGATCACGAGGGCCACGGCGTCGCACGCCTCGACGGCAAGGTCACCTTCGT
CGACGGCGCGCTGGCCGGCGAGCGCGCGGAAATCGCGATCTTTCGCAAGCACGCCAAGTACAACTCGGCCAATGCGGTCG
CGATTCTCGCGCCCAGCGCGCAGCGCGCAGAACCGCGTTGCCGCTATTTCGGCCGCTGCGGCGGCTGCTCGATGCAGCAT
CTCGAGCCGAGCGCCCAGGTCGCGGCCAAGCAACGCGTGCTCGAGGAAAACCTCGCCCGCATCGGCAAGGTCCGCCCCAG
TGTCCTGCTGCCGGCGCTGCACGGCCCGAGCTGGGGCTACCGCGGCCGCGCGCGCCTCTCGGTGCGCCGCGTCGAGAAGA
AAGGCGGCGTGCTGGTGGGCTTTCACGAGAAGCGCTCGAGCTTCATCGCCGACATGGCCAGCTGCGAGGTGCTCGCCCCC
GGCGTGTCGGCCCTGATCCAGCCGCTGCGCGAGCTGATCGGCCGCCTGTCGAACGCCGACCGCATCCCGCAGATCGAAGT
CGCCGCCGGCGAACACGTCATCGTGCTGGTGTTCCGCCTGCTCGAACCCTGGAACGACGACGATGCGGCGCACGTTCGCG
CATTCGCCGACGCGCACCACGTGCAGGTCTGGGAGCAGCGCAAGGGTCCGGAGACCGCGCGCCCGTTCTGGCCCGACATC
GCGCCGGAATTGAGCTACGGCCTGCCCGAATTCGGGCTGGTAATGCCTTTCCGGCCGACCGAGTTCACCCAAGTCAACAG
TGCGATCAACCGCGCGCTCGTGAGCCGCGCGCTGCGCCTGCTCGACCCGCGGCCAGGCGAACGCATCGCCGACCTGTTCT
GCGGCCTCGGCAACTTCACGCTGCCGATCGCGCACCGCGGCGCCGACGTCCTCGGCATCGAGGGCAGCACGGAGCTCGTC
GCCCGGGCGCGCGAAAACGCCCTGCGCAACGCCCTGCCCCACGCGCGATTCGAGGTCGACAACCTGTTCGAGATGACGCC
GGAAAAATTCGCCGCGCTCGGGCCCTTCGACAAGCTGTTGATCGACCCCCCGCGCTCGGGCGCGATCGAGGTCGTGAAGT
CGCTGCCGGAGGCCGGCGCGCCCCGCCGCATCGTCTACGTTTCCTGCGATCCAGCGACGCTCGCACGCGATGCCGAGGTG
CTCGTCCACGTCAAGGGCTACCGGCTGGAAGCGGCCGGCGTCGCCAACATGTTCCCTCACACCGCGCACGTCGAATCGAT
CGCGCTATTCGAGCGCTAA

Upstream 100 bases:

>100_bases
CGCTTTCCGAAGCCGCCTACCTCGCTGAAATCGCGCTTGCGCGCGCCACCGTCGAGGCGCATCCCGCGGCGCACTGGCGG
GAGTTTCTCGCGGCCTGGGC

Downstream 100 bases:

>100_bases
TCCCTTAGAATCCGTTTTTTTCGCCCCCAGCCCCTAACTCCTGGATCCCAGCCCCCAGAAATGGCACTCATCGTACAAAA
GTACGGCGGCACCTCGGTCG

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNPVVDILSLDHEGHGVARLDGKVTFVDGALAGERAEIAIFRKHAKYNSANAVAILAPSAQRAEPRCRYFGRCGGCSMQH
LEPSAQVAAKQRVLEENLARIGKVRPSVLLPALHGPSWGYRGRARLSVRRVEKKGGVLVGFHEKRSSFIADMASCEVLAP
GVSALIQPLRELIGRLSNADRIPQIEVAAGEHVIVLVFRLLEPWNDDDAAHVRAFADAHHVQVWEQRKGPETARPFWPDI
APELSYGLPEFGLVMPFRPTEFTQVNSAINRALVSRALRLLDPRPGERIADLFCGLGNFTLPIAHRGADVLGIEGSTELV
ARARENALRNALPHARFEVDNLFEMTPEKFAALGPFDKLLIDPPRSGAIEVVKSLPEAGAPRRIVYVSCDPATLARDAEV
LVHVKGYRLEAAGVANMFPHTAHVESIALFER

Sequences:

>Translated_432_residues
MNPVVDILSLDHEGHGVARLDGKVTFVDGALAGERAEIAIFRKHAKYNSANAVAILAPSAQRAEPRCRYFGRCGGCSMQH
LEPSAQVAAKQRVLEENLARIGKVRPSVLLPALHGPSWGYRGRARLSVRRVEKKGGVLVGFHEKRSSFIADMASCEVLAP
GVSALIQPLRELIGRLSNADRIPQIEVAAGEHVIVLVFRLLEPWNDDDAAHVRAFADAHHVQVWEQRKGPETARPFWPDI
APELSYGLPEFGLVMPFRPTEFTQVNSAINRALVSRALRLLDPRPGERIADLFCGLGNFTLPIAHRGADVLGIEGSTELV
ARARENALRNALPHARFEVDNLFEMTPEKFAALGPFDKLLIDPPRSGAIEVVKSLPEAGAPRRIVYVSCDPATLARDAEV
LVHVKGYRLEAAGVANMFPHTAHVESIALFER
>Mature_432_residues
MNPVVDILSLDHEGHGVARLDGKVTFVDGALAGERAEIAIFRKHAKYNSANAVAILAPSAQRAEPRCRYFGRCGGCSMQH
LEPSAQVAAKQRVLEENLARIGKVRPSVLLPALHGPSWGYRGRARLSVRRVEKKGGVLVGFHEKRSSFIADMASCEVLAP
GVSALIQPLRELIGRLSNADRIPQIEVAAGEHVIVLVFRLLEPWNDDDAAHVRAFADAHHVQVWEQRKGPETARPFWPDI
APELSYGLPEFGLVMPFRPTEFTQVNSAINRALVSRALRLLDPRPGERIADLFCGLGNFTLPIAHRGADVLGIEGSTELV
ARARENALRNALPHARFEVDNLFEMTPEKFAALGPFDKLLIDPPRSGAIEVVKSLPEAGAPRRIVYVSCDPATLARDAEV
LVHVKGYRLEAAGVANMFPHTAHVESIALFER

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=191, Percent_Identity=29.8429319371728, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI51173878, Length=191, Percent_Identity=29.8429319371728, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=37.2685185185185, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1787083, Length=175, Percent_Identity=33.7142857142857, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790403, Length=367, Percent_Identity=25.3405994550409, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666579, Length=205, Percent_Identity=31.7073170731707, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_THIDA (Q3SK67)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314728.1
- ProteinModelPortal:   Q3SK67
- SMR:   Q3SK67
- STRING:   Q3SK67
- GeneID:   3672764
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0970
- NMPDR:   fig|292415.3.peg.597
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   HRADGKV
- PhylomeDB:   Q3SK67
- BioCyc:   TDEN292415:TBD_0970-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 47338; Mature: 47338

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 389-389 BINDING 264-264 BINDING 293-293 BINDING 298-298 BINDING 314-314 BINDING 341-341 BINDING 362-362

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVVDILSLDHEGHGVARLDGKVTFVDGALAGERAEIAIFRKHAKYNSANAVAILAPSA
CCCCEEEEECCCCCCCEEEECCEEEEEECCCCCCCCEEEEEEHHCCCCCCCEEEEECCCC
QRAEPRCRYFGRCGGCSMQHLEPSAQVAAKQRVLEENLARIGKVRPSVLLPALHGPSWGY
CCCCCHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
RGRARLSVRRVEKKGGVLVGFHEKRSSFIADMASCEVLAPGVSALIQPLRELIGRLSNAD
CCCEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHCCCC
RIPQIEVAAGEHVIVLVFRLLEPWNDDDAAHVRAFADAHHVQVWEQRKGPETARPFWPDI
CCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
APELSYGLPEFGLVMPFRPTEFTQVNSAINRALVSRALRLLDPRPGERIADLFCGLGNFT
CCHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCE
LPIAHRGADVLGIEGSTELVARARENALRNALPHARFEVDNLFEMTPEKFAALGPFDKLL
EEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHCCCCHHHE
IDPPRSGAIEVVKSLPEAGAPRRIVYVSCDPATLARDAEVLVHVKGYRLEAAGVANMFPH
ECCCCCCHHHHHHHCCCCCCCCEEEEEECCCHHHHCCCEEEEEEECEEEECCCHHHHCCC
TAHVESIALFER
HHHHHHHHHCCC
>Mature Secondary Structure
MNPVVDILSLDHEGHGVARLDGKVTFVDGALAGERAEIAIFRKHAKYNSANAVAILAPSA
CCCCEEEEECCCCCCCEEEECCEEEEEECCCCCCCCEEEEEEHHCCCCCCCEEEEECCCC
QRAEPRCRYFGRCGGCSMQHLEPSAQVAAKQRVLEENLARIGKVRPSVLLPALHGPSWGY
CCCCCHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
RGRARLSVRRVEKKGGVLVGFHEKRSSFIADMASCEVLAPGVSALIQPLRELIGRLSNAD
CCCEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHCCCC
RIPQIEVAAGEHVIVLVFRLLEPWNDDDAAHVRAFADAHHVQVWEQRKGPETARPFWPDI
CCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
APELSYGLPEFGLVMPFRPTEFTQVNSAINRALVSRALRLLDPRPGERIADLFCGLGNFT
CCHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCE
LPIAHRGADVLGIEGSTELVARARENALRNALPHARFEVDNLFEMTPEKFAALGPFDKLL
EEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHCCCCHHHE
IDPPRSGAIEVVKSLPEAGAPRRIVYVSCDPATLARDAEVLVHVKGYRLEAAGVANMFPH
ECCCCCCHHHHHHHCCCCCCCCEEEEEECCCHHHHCCCEEEEEEECEEEECCCHHHHCCC
TAHVESIALFER
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA