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The map label for this gene is lysC [H]

Identifier: 74316987

GI number: 74316987

Start: 1032291

End: 1033520

Strand: Reverse

Name: lysC [H]

Synonym: Tbd_0969

Alternate gene names: 74316987

Gene position: 1033520-1032291 (Counterclockwise)

Preceding gene: 74316988

Following gene: 74316986

Centisome position: 35.52

GC content: 63.01

Gene sequence:

>1230_bases
ATGGCACTCATCGTACAAAAGTACGGCGGCACCTCGGTCGCCAACGTCGAACGCATCCGCGCCGTGGCCGAACGCGTCGC
CAAGTTCAAAATGCTCGGCCACCAGGTCGTGGTCGTGCTCTCGGCGATGTCGGGCGAAACCAACCGCCTGATCGGCCTCG
CCAAACAGATCCAGGCCGAACCCGACCCGCGCGAGCTCGACATGATGGTGTCGACTGGCGAGCAGGTCACGATCGCGCTG
CTCGCGATGGCGCTCAAGGATCTCGGCCTCAAGGCGAAGAGCTACACCGGCGCCCAGGTGCGCATCCTCACAGACGACGC
CTTCACCAAGGCCCGCATTCTCAGCATCGACGAGGCCAAGATGCGGCGCGACCTCGACTCGGGTCACGTCGTCGTCGTCG
CGGGCTTCCAAGGCGTCGACGAGGGCGGCAACATCACGACCTTGGGACGGGGCGGCTCTGACACCACCGGCGTCGCGCTC
GCGGCCGCGCTCAAGGCCGACGAATGCCAGATCTATACCGACGTCGACGGCGTCTATACGACCGACCCACGCATCGTCCC
CGAAGCACGGCGGCTGAAGACCGTGACGTTCGAGGAAATGCTCGAAATGGCGAGCCTCGGCTCCAAGGTGCTGCAGATCC
GCTCGGTCGAGTTCGCAGGCAAGTACAAGGTCAAACTGCGCGTGCTGTCGAGCTTTCAGGACGAAGGCGACGGCACGCTC
ATCACATTCGAGGAAAACGACAACATGGAACAGGCCATCATCTCGGGCATCGCCTTCAGCCGTGACGAAGCGAAGATCAC
TGTCGTCGGCGTGCCGGACCATCCGGGCATCGCCTATCAGATTCTTGGCCCCGTCGCCGACGCCAACGTCGACGTCGACA
TGATCGTGCAGAACGTCGGTCACGCCAACACCACCGACTTCACCTTCACCGTGCACCGCAACGACTTCGCCAAGGCGATG
AGCCTCGTCAAGGCGACCGCCGCGACGGTCGGCGCACGCGAAGTCACCGGTGACGACAAGATCGCCAAGGTGTCGATCGT
CGGTGTCGGCATGCGCTCGCACGTCGGCATCGCGAAAAAGATGTTCGAGACGCTGTCGAAGGAAAACATCAACCTGCAGA
TGATCTCGACCTCCGAGATCAAGATCTCGGTCGTGGTCGAGGACAAGTACCTCGAACTCGCCGTGCGCGCGCTGCACCAG
GCCTTCGAACTGGACAAGGCGACGGCCTGA

Upstream 100 bases:

>100_bases
CACCGCGCACGTCGAATCGATCGCGCTATTCGAGCGCTAATCCCTTAGAATCCGTTTTTTTCGCCCCCAGCCCCTAACTC
CTGGATCCCAGCCCCCAGAA

Downstream 100 bases:

>100_bases
AGTGGCTTTCCGCCGCGCATTCCGCTACAATGCGCGGCGGTTCGGAGACGTGGCCGAGTGGTTGAAGGTACTCCCCTGCT
AAGGGAGCATACGGGCTTAA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MALIVQKYGGTSVANVERIRAVAERVAKFKMLGHQVVVVLSAMSGETNRLIGLAKQIQAEPDPRELDMMVSTGEQVTIAL
LAMALKDLGLKAKSYTGAQVRILTDDAFTKARILSIDEAKMRRDLDSGHVVVVAGFQGVDEGGNITTLGRGGSDTTGVAL
AAALKADECQIYTDVDGVYTTDPRIVPEARRLKTVTFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQDEGDGTL
ITFEENDNMEQAIISGIAFSRDEAKITVVGVPDHPGIAYQILGPVADANVDVDMIVQNVGHANTTDFTFTVHRNDFAKAM
SLVKATAATVGAREVTGDDKIAKVSIVGVGMRSHVGIAKKMFETLSKENINLQMISTSEIKISVVVEDKYLELAVRALHQ
AFELDKATA

Sequences:

>Translated_409_residues
MALIVQKYGGTSVANVERIRAVAERVAKFKMLGHQVVVVLSAMSGETNRLIGLAKQIQAEPDPRELDMMVSTGEQVTIAL
LAMALKDLGLKAKSYTGAQVRILTDDAFTKARILSIDEAKMRRDLDSGHVVVVAGFQGVDEGGNITTLGRGGSDTTGVAL
AAALKADECQIYTDVDGVYTTDPRIVPEARRLKTVTFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQDEGDGTL
ITFEENDNMEQAIISGIAFSRDEAKITVVGVPDHPGIAYQILGPVADANVDVDMIVQNVGHANTTDFTFTVHRNDFAKAM
SLVKATAATVGAREVTGDDKIAKVSIVGVGMRSHVGIAKKMFETLSKENINLQMISTSEIKISVVVEDKYLELAVRALHQ
AFELDKATA
>Mature_408_residues
ALIVQKYGGTSVANVERIRAVAERVAKFKMLGHQVVVVLSAMSGETNRLIGLAKQIQAEPDPRELDMMVSTGEQVTIALL
AMALKDLGLKAKSYTGAQVRILTDDAFTKARILSIDEAKMRRDLDSGHVVVVAGFQGVDEGGNITTLGRGGSDTTGVALA
AALKADECQIYTDVDGVYTTDPRIVPEARRLKTVTFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQDEGDGTLI
TFEENDNMEQAIISGIAFSRDEAKITVVGVPDHPGIAYQILGPVADANVDVDMIVQNVGHANTTDFTFTVHRNDFAKAMS
LVKATAATVGAREVTGDDKIAKVSIVGVGMRSHVGIAKKMFETLSKENINLQMISTSEIKISVVVEDKYLELAVRALHQA
FELDKATA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=471, Percent_Identity=27.6008492569002, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790455, Length=456, Percent_Identity=27.4122807017544, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790376, Length=285, Percent_Identity=23.5087719298246, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=383, Percent_Identity=27.154046997389, Blast_Score=120, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44106; Mature: 43975

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVQKYGGTSVANVERIRAVAERVAKFKMLGHQVVVVLSAMSGETNRLIGLAKQIQAE
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCC
PDPRELDMMVSTGEQVTIALLAMALKDLGLKAKSYTGAQVRILTDDAFTKARILSIDEAK
CCHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCHHH
MRRDLDSGHVVVVAGFQGVDEGGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT
HHCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCEEE
TDPRIVPEARRLKTVTFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQDEGDGTL
CCCCCCCCHHHHEEECHHHHHHHHHCCHHHEEEEEEEECCEEEEEEEEEECCCCCCCCEE
ITFEENDNMEQAIISGIAFSRDEAKITVVGVPDHPGIAYQILGPVADANVDVDMIVQNVG
EEEECCCCHHHHHHHHHEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCC
HANTTDFTFTVHRNDFAKAMSLVKATAATVGAREVTGDDKIAKVSIVGVGMRSHVGIAKK
CCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEECHHHHHHHHHH
MFETLSKENINLQMISTSEIKISVVVEDKYLELAVRALHQAFELDKATA
HHHHHCCCCCEEEEEEECCEEEEEEEECHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALIVQKYGGTSVANVERIRAVAERVAKFKMLGHQVVVVLSAMSGETNRLIGLAKQIQAE
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCC
PDPRELDMMVSTGEQVTIALLAMALKDLGLKAKSYTGAQVRILTDDAFTKARILSIDEAK
CCHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCHHH
MRRDLDSGHVVVVAGFQGVDEGGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT
HHCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCEEE
TDPRIVPEARRLKTVTFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQDEGDGTL
CCCCCCCCHHHHEEECHHHHHHHHHCCHHHEEEEEEEECCEEEEEEEEEECCCCCCCCEE
ITFEENDNMEQAIISGIAFSRDEAKITVVGVPDHPGIAYQILGPVADANVDVDMIVQNVG
EEEECCCCHHHHHHHHHEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCC
HANTTDFTFTVHRNDFAKAMSLVKATAATVGAREVTGDDKIAKVSIVGVGMRSHVGIAKK
CCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEECHHHHHHHHHH
MFETLSKENINLQMISTSEIKISVVVEDKYLELAVRALHQAFELDKATA
HHHHHCCCCCEEEEEEECCEEEEEEEECHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]