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The map label for this gene is ampD [H]

Identifier: 74316794

GI number: 74316794

Start: 820966

End: 821517

Strand: Reverse

Name: ampD [H]

Synonym: Tbd_0776

Alternate gene names: 74316794

Gene position: 821517-820966 (Counterclockwise)

Preceding gene: 74316801

Following gene: 74316793

Centisome position: 28.23

GC content: 68.66

Gene sequence:

>552_bases
ATGCCGATTTCCGCACCCGACATGCCCGACGCGCTGGGCTGGTTGCCCGCTGCACGCCGGGTCCCCTCGCCCAATTGCGA
CCCGCGTCCGCCGGGAACGGCCGTCGAACTGATCGTCATCCACAACATTTCGCTGCCGCCCGGCGTCTTCGCCGGCGACG
CCGTGATCGAGCTCTTCACCAACCGCCTCGACTGGGACGCCGATCCCTATTACCAGGCGATGCGCGGGCTCAGGGTCTCC
GCCCATTTCTTCATCCGCCGCGACGGCAGCCTGCTGCAATTCGTGCCATGCACGCTGCGCGCCTGGCACGCGGGCGCATC
GAACTGGCAGGGGCGCGAACGCTGCAACGATTTCTCGATCGGCATCGAACTCGAAGGCAGCGACGACGTGCCTTTCGACG
ACGCGCAGTACGCGACCCTGCGCCGGCTCGTCGACGTGCTGAAGGCCGCCTATCCGATCCAGGGCGTCGCCGGCCACAGC
GACATCGCGCCCGGCCGCAAGAGCGACCCCGGCCCGCATTTCGCCTGGCACCGCCTCGACGCGACAGCCTGA

Upstream 100 bases:

>100_bases
GTGCGGTGTCATGAGCGGCGGTGAAATCGGCGAAAGTCGGCAGTCTAAGCCGTCGCCGCCGATGTTTCCAGCGCGCGCTC
GGGTCGAGTAAGCTTGCGCG

Downstream 100 bases:

>100_bases
AAAGCACCGACCCGCCTCGAATCGACGGCTCTCGCCGGCCGCACGTCCGCGGTAAAATTCCGGGTTTGCTCTTCAGGACT
CAGCCCATGCGTCTCGGCAC

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MPISAPDMPDALGWLPAARRVPSPNCDPRPPGTAVELIVIHNISLPPGVFAGDAVIELFTNRLDWDADPYYQAMRGLRVS
AHFFIRRDGSLLQFVPCTLRAWHAGASNWQGRERCNDFSIGIELEGSDDVPFDDAQYATLRRLVDVLKAAYPIQGVAGHS
DIAPGRKSDPGPHFAWHRLDATA

Sequences:

>Translated_183_residues
MPISAPDMPDALGWLPAARRVPSPNCDPRPPGTAVELIVIHNISLPPGVFAGDAVIELFTNRLDWDADPYYQAMRGLRVS
AHFFIRRDGSLLQFVPCTLRAWHAGASNWQGRERCNDFSIGIELEGSDDVPFDDAQYATLRRLVDVLKAAYPIQGVAGHS
DIAPGRKSDPGPHFAWHRLDATA
>Mature_182_residues
PISAPDMPDALGWLPAARRVPSPNCDPRPPGTAVELIVIHNISLPPGVFAGDAVIELFTNRLDWDADPYYQAMRGLRVSA
HFFIRRDGSLLQFVPCTLRAWHAGASNWQGRERCNDFSIGIELEGSDDVPFDDAQYATLRRLVDVLKAAYPIQGVAGHSD
IAPGRKSDPGPHFAWHRLDATA

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=169, Percent_Identity=55.0295857988166, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1787092, Length=132, Percent_Identity=39.3939393939394, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20124; Mature: 19993

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISAPDMPDALGWLPAARRVPSPNCDPRPPGTAVELIVIHNISLPPGVFAGDAVIELFT
CCCCCCCCCHHHHCCCHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
NRLDWDADPYYQAMRGLRVSAHFFIRRDGSLLQFVPCTLRAWHAGASNWQGRERCNDFSI
HHCCCCCCHHHHHHHCCEEEEEEEEECCCCEEEEHHHHHHHHHCCCCCCCCHHHCCCEEE
GIELEGSDDVPFDDAQYATLRRLVDVLKAAYPIQGVAGHSDIAPGRKSDPGPHFAWHRLD
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
ATA
CCC
>Mature Secondary Structure 
PISAPDMPDALGWLPAARRVPSPNCDPRPPGTAVELIVIHNISLPPGVFAGDAVIELFT
CCCCCCCCHHHHCCCHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
NRLDWDADPYYQAMRGLRVSAHFFIRRDGSLLQFVPCTLRAWHAGASNWQGRERCNDFSI
HHCCCCCCHHHHHHHCCEEEEEEEEECCCCEEEEHHHHHHHHHCCCCCCCCHHHCCCEEE
GIELEGSDDVPFDDAQYATLRRLVDVLKAAYPIQGVAGHSDIAPGRKSDPGPHFAWHRLD
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
ATA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294 [H]