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The map label for this gene is purT

Identifier: 74316793

GI number: 74316793

Start: 819683

End: 820879

Strand: Reverse

Name: purT

Synonym: Tbd_0775

Alternate gene names: 74316793

Gene position: 820879-819683 (Counterclockwise)

Preceding gene: 74316794

Following gene: 74316792

Centisome position: 28.21

GC content: 70.43

Gene sequence:

>1197_bases
ATGCGTCTCGGCACCCCGCTCTCGCCATCGGCGACCCGCGTCATGCTGCTCGGCGCCGGCGAACTCGGCAAGGAGGTCAT
CATCGCGCTGCAGCGTCTCGGCGTCGAAGTCGTCGCCGTCGATCGCTACGAGCATGCGCCCGGCCACCAGGTCGCGCATC
GCGCGCACGTGATCGACATGACCGACGGCGCCGCGCTTCGTGCGCTGATCGAGGCCGAAAAGCCGCTGCTCGTCGTCCCG
GAAATCGAGGCGATCGCGACCGAGGCCCTGCTCGAGATCGAGCGCGCCGGTCTCGCCGAGGTGATTCCAACCGCGCGCGC
CGTCAATCTGACCATGAACCGCGAGGGCATCCGCCGGCTCGCCGCCGAAACGCTCGGCTTGCCGACCTCGCCCTACGCGT
TCGTCGACAGCTACGCCGAGATGTGCGCCGCGGCCGAGCGGCTCGGCTATCCGGTCATCGTCAAGCCGGTCATGTCGTCG
TCGGGCAAGGGGCAATCGCGCGTCGACGACGCCGGCGAGATGCGGGCGGCCTGGGATTACGCCGTGCGCGGCGGGCGCGT
CGACAAGGGCCGCTTCATCGTCGAGGGCGTGATCGACTTCGACTACGAAATCACGCTGCTGACCGTGCGCGCACGCGCCG
CCTCGGGCGCGATCGAAACCCGTTTCTGCGCGCCGATCGGCCATTTGCAGGTCAAGGGCGATTACGTCGAATCGTGGCAG
CCGCAGGCGATGTCGCCGACCGCGCTCGAACGCGCGCGCGAAATCGCCGCGGCGGTCACGGGCGAGCTCGGTGGTCGCGG
GCTTTTCGGCGTCGAACTCTTCGTCAAGGGCGACACCGTGTGGTTTTCGGAAGTGAGCCCGCGGCCGCACGACACCGGCA
TGGTCACGCTCGCGACGCAGCGGCAGAACGAGTTCGAACTGCACGCGCGCGCGATCCTCGGGCTTCCGGTCGACACCGGC
CTGCGGGAACCCGGCGCGTCGGCCGTGATCTACGGCGCTGTGGACGCGCGAGGCATCGCGTTCGACGGCGTCGACGACGC
GCTCGCGGCCCCGGGCGTCGACCTGCGGCTCTTCGGCAAGCCCGAGGCCTTCGCCCGTCGCCGCATGGGCGTCGCGCTGG
CGACGGCCGACTCTCCCGACGCGGCCCGCCTGCTGGCGAAGGCCGCGGCGGCCAAGGTCAAACCCGTTCCCGTATGA

Upstream 100 bases:

>100_bases
ACGCGACAGCCTGAAAAGCACCGACCCGCCTCGAATCGACGGCTCTCGCCGGCCGCACGTCCGCGGTAAAATTCCGGGTT
TGCTCTTCAGGACTCAGCCC

Downstream 100 bases:

>100_bases
CTGGTGCGAACATGCAAACCCATTACGACCGCCTCGGCGGCGCCGACACGATCCGCAGGCTTGTCGACCGCTTTTACGAC
CTGATGGACGAGGACCCGGA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVVAVDRYEHAPGHQVAHRAHVIDMTDGAALRALIEAEKPLLVVP
EIEAIATEALLEIERAGLAEVIPTARAVNLTMNREGIRRLAAETLGLPTSPYAFVDSYAEMCAAAERLGYPVIVKPVMSS
SGKGQSRVDDAGEMRAAWDYAVRGGRVDKGRFIVEGVIDFDYEITLLTVRARAASGAIETRFCAPIGHLQVKGDYVESWQ
PQAMSPTALERAREIAAAVTGELGGRGLFGVELFVKGDTVWFSEVSPRPHDTGMVTLATQRQNEFELHARAILGLPVDTG
LREPGASAVIYGAVDARGIAFDGVDDALAAPGVDLRLFGKPEAFARRRMGVALATADSPDAARLLAKAAAAKVKPVPV

Sequences:

>Translated_398_residues
MRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVVAVDRYEHAPGHQVAHRAHVIDMTDGAALRALIEAEKPLLVVP
EIEAIATEALLEIERAGLAEVIPTARAVNLTMNREGIRRLAAETLGLPTSPYAFVDSYAEMCAAAERLGYPVIVKPVMSS
SGKGQSRVDDAGEMRAAWDYAVRGGRVDKGRFIVEGVIDFDYEITLLTVRARAASGAIETRFCAPIGHLQVKGDYVESWQ
PQAMSPTALERAREIAAAVTGELGGRGLFGVELFVKGDTVWFSEVSPRPHDTGMVTLATQRQNEFELHARAILGLPVDTG
LREPGASAVIYGAVDARGIAFDGVDDALAAPGVDLRLFGKPEAFARRRMGVALATADSPDAARLLAKAAAAKVKPVPV
>Mature_398_residues
MRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVVAVDRYEHAPGHQVAHRAHVIDMTDGAALRALIEAEKPLLVVP
EIEAIATEALLEIERAGLAEVIPTARAVNLTMNREGIRRLAAETLGLPTSPYAFVDSYAEMCAAAERLGYPVIVKPVMSS
SGKGQSRVDDAGEMRAAWDYAVRGGRVDKGRFIVEGVIDFDYEITLLTVRARAASGAIETRFCAPIGHLQVKGDYVESWQ
PQAMSPTALERAREIAAAVTGELGGRGLFGVELFVKGDTVWFSEVSPRPHDTGMVTLATQRQNEFELHARAILGLPVDTG
LREPGASAVIYGAVDARGIAFDGVDDALAAPGVDLRLFGKPEAFARRRMGVALATADSPDAARLLAKAAAAKVKPVPV

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=394, Percent_Identity=61.6751269035533, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786733, Length=270, Percent_Identity=31.4814814814815, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=371, Percent_Identity=24.5283018867925, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_THIDA (Q3SKP9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314533.1
- ProteinModelPortal:   Q3SKP9
- SMR:   Q3SKP9
- STRING:   Q3SKP9
- GeneID:   3673969
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0775
- NMPDR:   fig|292415.3.peg.40
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q3SKP9
- ProtClustDB:   PRK09288
- BioCyc:   TDEN292415:TBD_0775-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42357; Mature: 42357

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 81-81 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 272-272 BINDING 284-284 BINDING 291-291 BINDING 360-360

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVVAVDRYEHAPGHQVAHRAHVIDM
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHEEEEEEC
TDGAALRALIEAEKPLLVVPEIEAIATEALLEIERAGLAEVIPTARAVNLTMNREGIRRL
CCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEECHHHHHHH
AAETLGLPTSPYAFVDSYAEMCAAAERLGYPVIVKPVMSSSGKGQSRVDDAGEMRAAWDY
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHH
AVRGGRVDKGRFIVEGVIDFDYEITLLTVRARAASGAIETRFCAPIGHLQVKGDYVESWQ
HHCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCEEEECCCCCEEEECCHHHCCC
PQAMSPTALERAREIAAAVTGELGGRGLFGVELFVKGDTVWFSEVSPRPHDTGMVTLATQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCEEEEEEC
RQNEFELHARAILGLPVDTGLREPGASAVIYGAVDARGIAFDGVDDALAAPGVDLRLFGK
CCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCCHHHHCCCCCEEEECC
PEAFARRRMGVALATADSPDAARLLAKAAAAKVKPVPV
CHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVVAVDRYEHAPGHQVAHRAHVIDM
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHEEEEEEC
TDGAALRALIEAEKPLLVVPEIEAIATEALLEIERAGLAEVIPTARAVNLTMNREGIRRL
CCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEECHHHHHHH
AAETLGLPTSPYAFVDSYAEMCAAAERLGYPVIVKPVMSSSGKGQSRVDDAGEMRAAWDY
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHH
AVRGGRVDKGRFIVEGVIDFDYEITLLTVRARAASGAIETRFCAPIGHLQVKGDYVESWQ
HHCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCEEEECCCCCEEEECCHHHCCC
PQAMSPTALERAREIAAAVTGELGGRGLFGVELFVKGDTVWFSEVSPRPHDTGMVTLATQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCEEEEEEC
RQNEFELHARAILGLPVDTGLREPGASAVIYGAVDARGIAFDGVDDALAAPGVDLRLFGK
CCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCCHHHHCCCCCEEEECC
PEAFARRRMGVALATADSPDAARLLAKAAAAKVKPVPV
CHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA