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The map label for this gene is rpoN [H]

Identifier: 74316750

GI number: 74316750

Start: 774818

End: 776248

Strand: Reverse

Name: rpoN [H]

Synonym: Tbd_0732

Alternate gene names: 74316750

Gene position: 776248-774818 (Counterclockwise)

Preceding gene: 74316751

Following gene: 74316748

Centisome position: 26.68

GC content: 70.09

Gene sequence:

>1431_bases
ATGAATCGTCCCGCCCTCGCCCTCGAGCCGCGCCAGACGCAGACCCTGACGCCGCGGCTGCAACAGGCGGTGCGGCTGCT
GCAGCTTTCGACCTTCGATTTCGCGCTTGAGCTGCAGGAGCTCGTCAGCAGCAACCCGTTCCTCGAACTCGAGGATGGCG
AGTTCGCCGACGAAGACGCGGCCGCACCGGACACCGCCACGACCGCGGCCGACACGCCCCCGGCAGCCGACGGGGCGCAG
GCGGAAGACTATGCCGAGACCGCTTACGAGGAGCGGCTCGGCGACGGCGGCGAGGCGCCGGGGCGGTACAGCGAGGCGAA
TGCCGACGCGATCGACTTCGCCTGCGCGCCGGTCACGCTGCGCCAGCACGTGCACAGCCAGCTCAACATGCTCGACCTGC
CGCCGCGCGAGCACTTTCTCGCCTGGATCATCGCCGAGGCGCTCGACGACGACGGCTACCTGCGCGTCGAACTCGACGCC
TTGCGCGCGATGGCGGGGCTCGAACCGCGTGTCGACGCGCGCGAGATGCTCGCGGCGCTCACGCGCGTGCAATCCCTGGA
CCCGGCGGGCCTCGCGGCCCGCGACGTCCCCGAGTGCCTGACCCTGCAGTTGCGGCGTCGGCCGCCGTCGCCGCAACGCG
AGCTCGCGCTGCGCATCGTGCGCGACGAACTCAAGCTGCTGCAGCAGCGCGACCTGCAGCGCATCGCCCAGCGCGTCGGC
AGCACTCCCGCGGAAGTGGAGGCCGTGTGCGCCTACATCCGCCGCCTCGAGCCGCGGCCCGGCGCGTGCTTCGGCCACCA
CGTCGCCGCGGCCATCCGCCCCGACGTGATCGTGCGCAAGATTCGCGGGCAGTGGACGGCCCTGCTGAACAGTTCGGCGG
TTCCGCGCGTCAGCCTCAACCGCACCTACGCCGAGCTGTTCCAACGCCACCGCGAGCCGCACCACGGCGACCTCGCCGGC
CACCTGCGCGAAGCGCGCTGGGCGGTGCAGAACATGGAGCAGCGCTTCTCGACGATCCTGCGCGTGGCGCAGGCCCTGGT
CGACCGCCAGCAGCATTTCTTCGAGCACGGCACGCTCGCCATGCAGCCGCTCGGCCTCAAGGACATCGCCGACGAACTGG
GCCTGCACGAGTCGACCGTCTCGCGCGCGACCTGCAACAAATTCATGGTGACGCCGCTGGGCGTGTTCGAACTGAAGTAT
TTCTTCTCGCGCGCGCTGAACACCGAACAGGGCAGTCAGTGCTCGACCACGGCGATCCGCGAGGCGATCCGCAAGATGAT
TCTTTCGGAGGATGCCCGCTCGCCGCACTCGGACGCCGACATCGCGCGCATCCTGATGCAGCAGGGCCTGCGCGTCGCGC
GGCGCACGGTCACCAAGTACCGCCAGATGATGGCGATCCCCAACGTCGACATGCGCCGCTACCACGGCTGA

Upstream 100 bases:

>100_bases
CGAGTATTCCGTGCGACGCTGCGATGTCCGTTCCTCCCGTGCATGCGCTGCCGCGGTGCCGGCTGCGCGTCGCCGCAGCT
TTCGGCCAGGAGGCCTGCGG

Downstream 100 bases:

>100_bases
GCCGGCCGCAGCCGGCGAAGACTAGCGCCGCTTCGGCGCGTCGCCCGTGTCCTGCGTGCCGCCGTCACTTTTGTCGGCGT
CGCCCGGCGCGCGCTTGCTG

Product: RNA polymease sigma-54 factor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNRPALALEPRQTQTLTPRLQQAVRLLQLSTFDFALELQELVSSNPFLELEDGEFADEDAAAPDTATTAADTPPAADGAQ
AEDYAETAYEERLGDGGEAPGRYSEANADAIDFACAPVTLRQHVHSQLNMLDLPPREHFLAWIIAEALDDDGYLRVELDA
LRAMAGLEPRVDAREMLAALTRVQSLDPAGLAARDVPECLTLQLRRRPPSPQRELALRIVRDELKLLQQRDLQRIAQRVG
STPAEVEAVCAYIRRLEPRPGACFGHHVAAAIRPDVIVRKIRGQWTALLNSSAVPRVSLNRTYAELFQRHREPHHGDLAG
HLREARWAVQNMEQRFSTILRVAQALVDRQQHFFEHGTLAMQPLGLKDIADELGLHESTVSRATCNKFMVTPLGVFELKY
FFSRALNTEQGSQCSTTAIREAIRKMILSEDARSPHSDADIARILMQQGLRVARRTVTKYRQMMAIPNVDMRRYHG

Sequences:

>Translated_476_residues
MNRPALALEPRQTQTLTPRLQQAVRLLQLSTFDFALELQELVSSNPFLELEDGEFADEDAAAPDTATTAADTPPAADGAQ
AEDYAETAYEERLGDGGEAPGRYSEANADAIDFACAPVTLRQHVHSQLNMLDLPPREHFLAWIIAEALDDDGYLRVELDA
LRAMAGLEPRVDAREMLAALTRVQSLDPAGLAARDVPECLTLQLRRRPPSPQRELALRIVRDELKLLQQRDLQRIAQRVG
STPAEVEAVCAYIRRLEPRPGACFGHHVAAAIRPDVIVRKIRGQWTALLNSSAVPRVSLNRTYAELFQRHREPHHGDLAG
HLREARWAVQNMEQRFSTILRVAQALVDRQQHFFEHGTLAMQPLGLKDIADELGLHESTVSRATCNKFMVTPLGVFELKY
FFSRALNTEQGSQCSTTAIREAIRKMILSEDARSPHSDADIARILMQQGLRVARRTVTKYRQMMAIPNVDMRRYHG
>Mature_476_residues
MNRPALALEPRQTQTLTPRLQQAVRLLQLSTFDFALELQELVSSNPFLELEDGEFADEDAAAPDTATTAADTPPAADGAQ
AEDYAETAYEERLGDGGEAPGRYSEANADAIDFACAPVTLRQHVHSQLNMLDLPPREHFLAWIIAEALDDDGYLRVELDA
LRAMAGLEPRVDAREMLAALTRVQSLDPAGLAARDVPECLTLQLRRRPPSPQRELALRIVRDELKLLQQRDLQRIAQRVG
STPAEVEAVCAYIRRLEPRPGACFGHHVAAAIRPDVIVRKIRGQWTALLNSSAVPRVSLNRTYAELFQRHREPHHGDLAG
HLREARWAVQNMEQRFSTILRVAQALVDRQQHFFEHGTLAMQPLGLKDIADELGLHESTVSRATCNKFMVTPLGVFELKY
FFSRALNTEQGSQCSTTAIREAIRKMILSEDARSPHSDADIARILMQQGLRVARRTVTKYRQMMAIPNVDMRRYHG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=485, Percent_Identity=37.5257731958763, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53364; Mature: 53364

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPALALEPRQTQTLTPRLQQAVRLLQLSTFDFALELQELVSSNPFLELEDGEFADEDA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
AAPDTATTAADTPPAADGAQAEDYAETAYEERLGDGGEAPGRYSEANADAIDFACAPVTL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCHHHH
RQHVHSQLNMLDLPPREHFLAWIIAEALDDDGYLRVELDALRAMAGLEPRVDAREMLAAL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHH
TRVQSLDPAGLAARDVPECLTLQLRRRPPSPQRELALRIVRDELKLLQQRDLQRIAQRVG
HHHHCCCCCCCCHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
STPAEVEAVCAYIRRLEPRPGACFGHHVAAAIRPDVIVRKIRGQWTALLNSSAVPRVSLN
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RTYAELFQRHREPHHGDLAGHLREARWAVQNMEQRFSTILRVAQALVDRQQHFFEHGTLA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
MQPLGLKDIADELGLHESTVSRATCNKFMVTPLGVFELKYFFSRALNTEQGSQCSTTAIR
ECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EAIRKMILSEDARSPHSDADIARILMQQGLRVARRTVTKYRQMMAIPNVDMRRYHG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MNRPALALEPRQTQTLTPRLQQAVRLLQLSTFDFALELQELVSSNPFLELEDGEFADEDA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
AAPDTATTAADTPPAADGAQAEDYAETAYEERLGDGGEAPGRYSEANADAIDFACAPVTL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCHHHH
RQHVHSQLNMLDLPPREHFLAWIIAEALDDDGYLRVELDALRAMAGLEPRVDAREMLAAL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHH
TRVQSLDPAGLAARDVPECLTLQLRRRPPSPQRELALRIVRDELKLLQQRDLQRIAQRVG
HHHHCCCCCCCCHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
STPAEVEAVCAYIRRLEPRPGACFGHHVAAAIRPDVIVRKIRGQWTALLNSSAVPRVSLN
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RTYAELFQRHREPHHGDLAGHLREARWAVQNMEQRFSTILRVAQALVDRQQHFFEHGTLA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
MQPLGLKDIADELGLHESTVSRATCNKFMVTPLGVFELKYFFSRALNTEQGSQCSTTAIR
ECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EAIRKMILSEDARSPHSDADIARILMQQGLRVARRTVTKYRQMMAIPNVDMRRYHG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]