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The map label for this gene is flbD [H]

Identifier: 74316748

GI number: 74316748

Start: 772372

End: 773538

Strand: Reverse

Name: flbD [H]

Synonym: Tbd_0730

Alternate gene names: 74316748

Gene position: 773538-772372 (Counterclockwise)

Preceding gene: 74316750

Following gene: 74316747

Centisome position: 26.58

GC content: 66.75

Gene sequence:

>1167_bases
ATGAAAACAGAGCAGGCAAACAAGGAGCGCGTTGTGCCCAAACCCATCCGCCACGAGTCACCCGACATGGGGATACCCAC
GCTCTATTCGTATGCGCGCGACGGGGGATCCCCGTCGCGCCCGGTCGAGCCGGCCGCACCGCCCACGGGCGCCGACCCCG
GCGAGCATGCCTTCGGCCGTCTGTTCGGCGCATCCCCCGCCATGCAGGATGTGTTCCACCTGATCGAGCGGGTCGCGCCG
AGCTACGTGTCAGTGCTGATCGTCGGCGAGAGCGGCTGCGGCAAGGAGCTGATCGCGCAGACACTGCACGCCATGAGCCG
CTGTTCGCACGCGCCCTTCGTCGCGCTGAACTGCGGCGCCCTGCCGCCCAATCTCGTCGAGGCCGAACTCTTCGGTTATG
AAAAAGGTGCATTCACCGGCGCAAACCGTACACACCAGGGCTTCTTCGAACGCGCCCACGGCGGCACGCTCTTCCTCGAC
GAGATCACCGAAATGCCGATCGATCTCCAGGTCCGCCTGCTGCGCGTGCTCGAAACCGGCCGCGTGATCCGCGTCGGCGG
CGACCAGGAAATCGAATGCAACGTGCGCGTGCTCGCGGCGACCAACCGCGATCCGATGGAAGCGCTGCGCGACAATCGCC
TGCGCGAAGACCTGCTGTACCGGCTCGCGGTGTTTCCGATCGTCGTGCCGCCGCTGCGCGAGCGCGAGGACGACGTCATC
CTGCTCGCCAACGCCTTCCTGAAGGAACTCAACGCCGAGTACGGCACGGCCAAGGCGTTCGCCGCCGACGCGGCCGACAA
GCTCCGCTCGCACAGCTGGCCCGGCAACGTGCGCGAACTCAAGAACTGCATCCAGCGCGCCTACATCATGGCCGACGGCA
TCGTCGAGATCGACACGCTCGCGCCGCTCGGCCAGAACGAGCGCGCCGGCTCGCGCCAGGTCGTCGAATTCGAGATCGGC
ACCTCGCTCGAGGAAGTCGAGCAGCAACTGATCATCGCGACGCTCGACCGTTTCCGCGGCGACAAGCGGCGCGCGGCCAA
GGCGCTCGGCGTGAGCCTCAAGACCATCTACAACCGCCTCAACGAATACGCCTCGGACGCCTCGCTGCTCGGCGGCGACG
AAGGTCCGCTCGTGCCGGCCGTGAGTTCGGCAGGCTCGCAGGTATAA

Upstream 100 bases:

>100_bases
ACAGGGCCGGGGGTGTAGGAATCATCCTGCTTCCGAATACGCGTTTGTCTCATTCGGCTGCCGGCCCGCGCGATTAGCAT
GAAGACCTCAAAACCGCGTC

Downstream 100 bases:

>100_bases
TCCCCGCCCACGGAAGGTTGGCAGAGCGGTTGAATGCACCGGTCTTGTTCGATCGAGCGCTGCTGTGGAAACGCAGCAGA
TGTAAGGAGTCAAAGTCGGC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKTEQANKERVVPKPIRHESPDMGIPTLYSYARDGGSPSRPVEPAAPPTGADPGEHAFGRLFGASPAMQDVFHLIERVAP
SYVSVLIVGESGCGKELIAQTLHAMSRCSHAPFVALNCGALPPNLVEAELFGYEKGAFTGANRTHQGFFERAHGGTLFLD
EITEMPIDLQVRLLRVLETGRVIRVGGDQEIECNVRVLAATNRDPMEALRDNRLREDLLYRLAVFPIVVPPLREREDDVI
LLANAFLKELNAEYGTAKAFAADAADKLRSHSWPGNVRELKNCIQRAYIMADGIVEIDTLAPLGQNERAGSRQVVEFEIG
TSLEEVEQQLIIATLDRFRGDKRRAAKALGVSLKTIYNRLNEYASDASLLGGDEGPLVPAVSSAGSQV

Sequences:

>Translated_388_residues
MKTEQANKERVVPKPIRHESPDMGIPTLYSYARDGGSPSRPVEPAAPPTGADPGEHAFGRLFGASPAMQDVFHLIERVAP
SYVSVLIVGESGCGKELIAQTLHAMSRCSHAPFVALNCGALPPNLVEAELFGYEKGAFTGANRTHQGFFERAHGGTLFLD
EITEMPIDLQVRLLRVLETGRVIRVGGDQEIECNVRVLAATNRDPMEALRDNRLREDLLYRLAVFPIVVPPLREREDDVI
LLANAFLKELNAEYGTAKAFAADAADKLRSHSWPGNVRELKNCIQRAYIMADGIVEIDTLAPLGQNERAGSRQVVEFEIG
TSLEEVEQQLIIATLDRFRGDKRRAAKALGVSLKTIYNRLNEYASDASLLGGDEGPLVPAVSSAGSQV
>Mature_388_residues
MKTEQANKERVVPKPIRHESPDMGIPTLYSYARDGGSPSRPVEPAAPPTGADPGEHAFGRLFGASPAMQDVFHLIERVAP
SYVSVLIVGESGCGKELIAQTLHAMSRCSHAPFVALNCGALPPNLVEAELFGYEKGAFTGANRTHQGFFERAHGGTLFLD
EITEMPIDLQVRLLRVLETGRVIRVGGDQEIECNVRVLAATNRDPMEALRDNRLREDLLYRLAVFPIVVPPLREREDDVI
LLANAFLKELNAEYGTAKAFAADAADKLRSHSWPGNVRELKNCIQRAYIMADGIVEIDTLAPLGQNERAGSRQVVEFEIG
TSLEEVEQQLIIATLDRFRGDKRRAAKALGVSLKTIYNRLNEYASDASLLGGDEGPLVPAVSSAGSQV

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=317, Percent_Identity=43.217665615142, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=40, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1790437, Length=314, Percent_Identity=43.9490445859873, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1790299, Length=326, Percent_Identity=41.7177914110429, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082117, Length=317, Percent_Identity=42.2712933753943, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=50.6437768240343, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1789087, Length=319, Percent_Identity=41.692789968652, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=41.4860681114551, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788905, Length=329, Percent_Identity=39.8176291793313, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87081872, Length=306, Percent_Identity=39.2156862745098, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=37.6996805111821, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI87081858, Length=339, Percent_Identity=30.6784660766962, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=30.3643724696356, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 42365; Mature: 42365

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEQANKERVVPKPIRHESPDMGIPTLYSYARDGGSPSRPVEPAAPPTGADPGEHAFGR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LFGASPAMQDVFHLIERVAPSYVSVLIVGESGCGKELIAQTLHAMSRCSHAPFVALNCGA
HHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
LPPNLVEAELFGYEKGAFTGANRTHQGFFERAHGGTLFLDEITEMPIDLQVRLLRVLETG
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHCC
RVIRVGGDQEIECNVRVLAATNRDPMEALRDNRLREDLLYRLAVFPIVVPPLREREDDVI
CEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LLANAFLKELNAEYGTAKAFAADAADKLRSHSWPGNVRELKNCIQRAYIMADGIVEIDTL
EEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC
APLGQNERAGSRQVVEFEIGTSLEEVEQQLIIATLDRFRGDKRRAAKALGVSLKTIYNRL
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NEYASDASLLGGDEGPLVPAVSSAGSQV
HHHHCCHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTEQANKERVVPKPIRHESPDMGIPTLYSYARDGGSPSRPVEPAAPPTGADPGEHAFGR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LFGASPAMQDVFHLIERVAPSYVSVLIVGESGCGKELIAQTLHAMSRCSHAPFVALNCGA
HHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
LPPNLVEAELFGYEKGAFTGANRTHQGFFERAHGGTLFLDEITEMPIDLQVRLLRVLETG
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHCC
RVIRVGGDQEIECNVRVLAATNRDPMEALRDNRLREDLLYRLAVFPIVVPPLREREDDVI
CEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LLANAFLKELNAEYGTAKAFAADAADKLRSHSWPGNVRELKNCIQRAYIMADGIVEIDTL
EEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC
APLGQNERAGSRQVVEFEIGTSLEEVEQQLIIATLDRFRGDKRRAAKALGVSLKTIYNRL
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NEYASDASLLGGDEGPLVPAVSSAGSQV
HHHHCCHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]