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The map label for this gene is garL [H]

Identifier: 74316734

GI number: 74316734

Start: 755542

End: 756303

Strand: Reverse

Name: garL [H]

Synonym: Tbd_0716

Alternate gene names: 74316734

Gene position: 756303-755542 (Counterclockwise)

Preceding gene: 74316735

Following gene: 74316733

Centisome position: 25.99

GC content: 69.03

Gene sequence:

>762_bases
ATGCTGCAGACCAATCGCCTCAAACGCGCGCTCGCCGACGGCAAGTCCGTGTTCGGCCTGCTCAATTCGATTCCGTCGCC
GCTCGTCGTCGAAATGATCGGCTACGCCGGATTCGATTTCGTCGTCCTCGACATGGAACACGTCTGCGTCAATCCGGAGA
CGCTCGAAAACATGGTACGCGCGGCCGAATGCGCCGGCATCACGCCGCTGGTCCGCGTACCAAGTGCCGCGCCCGACGTC
ATCCTGCGCGCGCTCGACTGCGGCGCGCAGGGCGTCGTCGTGCCGCACGTGCAGAGCGCGGCCGAAGCGGCGCAGGCCGT
CGCCGCGAGCCGCTACGCGCCGCTCGGCACGCGCGGCATCAGCGGCGGCCGCACGACCGGCTTCGGCACCACCGATCTGG
CGGCTTATCTCGCGCGCGCCAACGCGGAAATCATGGTCGTCGCGATGATCGAAGACCGCGCGGGCGTCGACGCCAGTGAC
GCGATCCTGTCGGTTGCCGGCATCGACATGGTTCTCGAAGGCGCGATCGACCTGTCTCAATCCTACGGCGTGCCGGGACA
GGCGCAGCACCCGATCGTGCAGGAAGCGATCGCGAAGGTTGCGGCCGCGTGTGCCGCGCGCGGCGTTCCGTTTTGCGCGA
TCCCGCGCGTCGCCGAGCAGACGGCCGCCTGGAAAGCGCGCGGCGTGCAGGCGTATCTGCTCGGCGACGACCGCGGCGTG
AGCTTCCGCGCGCTCAAGGCGCATCTCGCGGCCTTCCGCTGA

Upstream 100 bases:

>100_bases
TCTTTCCCTACGCCGGCGCCTATGCCTGGCACATCTCGCATCACGACTTCCTGCGCCACCCGCATCCGGCGCAGTGGTAC
CTGCCCGTGGAGTGACGCGC

Downstream 100 bases:

>100_bases
GCCCTGCGGCCAAACGGGAGAAATCCCCTCAAGGCAGCCGTGGTTTTGACATCGCTAATAAGAACCATTATTATTCGCAT
CGCCTCGATTCCGCGCCACG

Product: putative aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLQTNRLKRALADGKSVFGLLNSIPSPLVVEMIGYAGFDFVVLDMEHVCVNPETLENMVRAAECAGITPLVRVPSAAPDV
ILRALDCGAQGVVVPHVQSAAEAAQAVAASRYAPLGTRGISGGRTTGFGTTDLAAYLARANAEIMVVAMIEDRAGVDASD
AILSVAGIDMVLEGAIDLSQSYGVPGQAQHPIVQEAIAKVAAACAARGVPFCAIPRVAEQTAAWKARGVQAYLLGDDRGV
SFRALKAHLAAFR

Sequences:

>Translated_253_residues
MLQTNRLKRALADGKSVFGLLNSIPSPLVVEMIGYAGFDFVVLDMEHVCVNPETLENMVRAAECAGITPLVRVPSAAPDV
ILRALDCGAQGVVVPHVQSAAEAAQAVAASRYAPLGTRGISGGRTTGFGTTDLAAYLARANAEIMVVAMIEDRAGVDASD
AILSVAGIDMVLEGAIDLSQSYGVPGQAQHPIVQEAIAKVAAACAARGVPFCAIPRVAEQTAAWKARGVQAYLLGDDRGV
SFRALKAHLAAFR
>Mature_253_residues
MLQTNRLKRALADGKSVFGLLNSIPSPLVVEMIGYAGFDFVVLDMEHVCVNPETLENMVRAAECAGITPLVRVPSAAPDV
ILRALDCGAQGVVVPHVQSAAEAAQAVAASRYAPLGTRGISGGRTTGFGTTDLAAYLARANAEIMVVAMIEDRAGVDASD
AILSVAGIDMVLEGAIDLSQSYGVPGQAQHPIVQEAIAKVAAACAARGVPFCAIPRVAEQTAAWKARGVQAYLLGDDRGV
SFRALKAHLAAFR

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=36.0323886639676, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788578, Length=259, Percent_Identity=32.8185328185328, Blast_Score=120, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 26281; Mature: 26281

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTNRLKRALADGKSVFGLLNSIPSPLVVEMIGYAGFDFVVLDMEHVCVNPETLENMVR
CCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHH
AAECAGITPLVRVPSAAPDVILRALDCGAQGVVVPHVQSAAEAAQAVAASRYAPLGTRGI
HHHHCCCCHHHCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCC
SGGRTTGFGTTDLAAYLARANAEIMVVAMIEDRAGVDASDAILSVAGIDMVLEGAIDLSQ
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SYGVPGQAQHPIVQEAIAKVAAACAARGVPFCAIPRVAEQTAAWKARGVQAYLLGDDRGV
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
SFRALKAHLAAFR
HHHHHHHHHHHCC
>Mature Secondary Structure
MLQTNRLKRALADGKSVFGLLNSIPSPLVVEMIGYAGFDFVVLDMEHVCVNPETLENMVR
CCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHH
AAECAGITPLVRVPSAAPDVILRALDCGAQGVVVPHVQSAAEAAQAVAASRYAPLGTRGI
HHHHCCCCHHHCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCC
SGGRTTGFGTTDLAAYLARANAEIMVVAMIEDRAGVDASDAILSVAGIDMVLEGAIDLSQ
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SYGVPGQAQHPIVQEAIAKVAAACAARGVPFCAIPRVAEQTAAWKARGVQAYLLGDDRGV
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
SFRALKAHLAAFR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA