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The map label for this gene is truB

Identifier: 74316713

GI number: 74316713

Start: 734853

End: 735749

Strand: Reverse

Name: truB

Synonym: Tbd_0695

Alternate gene names: 74316713

Gene position: 735749-734853 (Counterclockwise)

Preceding gene: 74316714

Following gene: 74316712

Centisome position: 25.29

GC content: 70.35

Gene sequence:

>897_bases
GTGAAGCCCGCCCGCCAAGCTGTCGATGGCGTGCTGCTGCTGAACAAGCCGGTCGGGATCACCTCCAACGCCGCGCTGCA
GAAGGCCAAGTGGCTGCTGAACGCGAAGAAGGCCGGCCACACCGGTACGCTCGACCCCTTCGCCGACGGCCTCCTGCCGC
TCTGCTTCGGCGAGGCGACCAAGTTCTCGGCCTATCTGCTCGACGCCGACAAGCGCTATCGCGCGATCCTGCAACTCGGC
GTCACGACGCGCACCGGCGACCCCGAGGGCGAGGTGCTCGCGACCCGCGAAGTTCGCGCGACCTGCGCCGACATCCGCGC
GGCGCTTCCCGCTTTCGTCGGTGAAATCGAACAGATCCCGCCGATGCATTCGGCACTCAAGCATCAGGGGCGTCCGCTCT
ACGAGTACGCGCGTGCCGGCGTCGAGATCGCGCGTGCGCCGCGCCGGGTGCATATCCGCGCCCTCGACCTCTTCAAATGC
GCCCCGCCCCGCGCGGTCGTCGACGTGCAATGCAGCGCGGGCACCTACGTCCGCACGCTGGCCGAAGACCTCGGCAACGC
GCTCGGCTGCGGCGCCCACCTCACGGCCCTCACCCGCACCGCGTCGGGCGGATTCCTGCTCGAACAGGCACACACGCTCG
CCGAACTCGAGGCGACCAACGCGGGGGCCAGGCAGGCCCTGCTGCTACCCGCGGACTGCCTCGTCGCGCATCTGCCGAGC
GTGCACCTCGGCGAGACCGCCGCCGCGTCCTTGCGCCAGGGCCGGAGCGTGCCCGACGCTGCGGATCGCCGTGGACTCGT
GCGCGTGTACGACGGCCACGGGGTATTTGTCGGTCTCGCCGAAGCGGAAGCCGGAAAGCTGGTCCCGCGACGCCTGATCG
CCACCGTGCAGGCTTGA

Upstream 100 bases:

>100_bases
AGCTGACCTTCGTCTACGACCGCTCGGTCGAGCATGGCATGGCGCTGAGCCGGCTCATCGAAACGGCGGTCGCCGAAGAC
GCCAAGCACCCCAAGGACGA

Downstream 100 bases:

>100_bases
GCCGGACCATCCTTTGCGCGTAAAATGCGCCGTTTCCCACGAACGTGTCCCGCTCAGCCGCAGGCCACGCGATTTCTAAG
GAGTACACCATGGCTGTCAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKPARQAVDGVLLLNKPVGITSNAALQKAKWLLNAKKAGHTGTLDPFADGLLPLCFGEATKFSAYLLDADKRYRAILQLG
VTTRTGDPEGEVLATREVRATCADIRAALPAFVGEIEQIPPMHSALKHQGRPLYEYARAGVEIARAPRRVHIRALDLFKC
APPRAVVDVQCSAGTYVRTLAEDLGNALGCGAHLTALTRTASGGFLLEQAHTLAELEATNAGARQALLLPADCLVAHLPS
VHLGETAAASLRQGRSVPDAADRRGLVRVYDGHGVFVGLAEAEAGKLVPRRLIATVQA

Sequences:

>Translated_298_residues
MKPARQAVDGVLLLNKPVGITSNAALQKAKWLLNAKKAGHTGTLDPFADGLLPLCFGEATKFSAYLLDADKRYRAILQLG
VTTRTGDPEGEVLATREVRATCADIRAALPAFVGEIEQIPPMHSALKHQGRPLYEYARAGVEIARAPRRVHIRALDLFKC
APPRAVVDVQCSAGTYVRTLAEDLGNALGCGAHLTALTRTASGGFLLEQAHTLAELEATNAGARQALLLPADCLVAHLPS
VHLGETAAASLRQGRSVPDAADRRGLVRVYDGHGVFVGLAEAEAGKLVPRRLIATVQA
>Mature_298_residues
MKPARQAVDGVLLLNKPVGITSNAALQKAKWLLNAKKAGHTGTLDPFADGLLPLCFGEATKFSAYLLDADKRYRAILQLG
VTTRTGDPEGEVLATREVRATCADIRAALPAFVGEIEQIPPMHSALKHQGRPLYEYARAGVEIARAPRRVHIRALDLFKC
APPRAVVDVQCSAGTYVRTLAEDLGNALGCGAHLTALTRTASGGFLLEQAHTLAELEATNAGARQALLLPADCLVAHLPS
VHLGETAAASLRQGRSVPDAADRRGLVRVYDGHGVFVGLAEAEAGKLVPRRLIATVQA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=249, Percent_Identity=35.7429718875502, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI2367200, Length=312, Percent_Identity=43.9102564102564, Blast_Score=250, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17553978, Length=194, Percent_Identity=31.4432989690722, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=198, Percent_Identity=32.8282828282828, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=196, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364189, Length=194, Percent_Identity=30.9278350515464, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364187, Length=194, Percent_Identity=30.9278350515464, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364185, Length=194, Percent_Identity=30.9278350515464, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364183, Length=194, Percent_Identity=30.9278350515464, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62471759, Length=194, Percent_Identity=30.9278350515464, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17975520, Length=194, Percent_Identity=30.9278350515464, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_THIDA (Q3SKX3)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314453.1
- ProteinModelPortal:   Q3SKX3
- SMR:   Q3SKX3
- STRING:   Q3SKX3
- GeneID:   3672663
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0695
- NMPDR:   fig|292415.3.peg.789
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q3SKX3
- BioCyc:   TDEN292415:TBD_0695-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 31608; Mature: 31608

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPARQAVDGVLLLNKPVGITSNAALQKAKWLLNAKKAGHTGTLDPFADGLLPLCFGEAT
CCCHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCH
KFSAYLLDADKRYRAILQLGVTTRTGDPEGEVLATREVRATCADIRAALPAFVGEIEQIP
HEEEEEECCHHHHHHHHHHCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
PMHSALKHQGRPLYEYARAGVEIARAPRRVHIRALDLFKCAPPRAVVDVQCSAGTYVRTL
CHHHHHHHCCCCHHHHHHCCHHHHCCCCEEEEEEEHHHHCCCCCEEEEEEECCCHHHHHH
AEDLGNALGCGAHLTALTRTASGGFLLEQAHTLAELEATNAGARQALLLPADCLVAHLPS
HHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCC
VHLGETAAASLRQGRSVPDAADRRGLVRVYDGHGVFVGLAEAEAGKLVPRRLIATVQA
CCCCHHHHHHHHHCCCCCCHHHCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKPARQAVDGVLLLNKPVGITSNAALQKAKWLLNAKKAGHTGTLDPFADGLLPLCFGEAT
CCCHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCH
KFSAYLLDADKRYRAILQLGVTTRTGDPEGEVLATREVRATCADIRAALPAFVGEIEQIP
HEEEEEECCHHHHHHHHHHCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
PMHSALKHQGRPLYEYARAGVEIARAPRRVHIRALDLFKCAPPRAVVDVQCSAGTYVRTL
CHHHHHHHCCCCHHHHHHCCHHHHCCCCEEEEEEEHHHHCCCCCEEEEEEECCCHHHHHH
AEDLGNALGCGAHLTALTRTASGGFLLEQAHTLAELEATNAGARQALLLPADCLVAHLPS
HHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCC
VHLGETAAASLRQGRSVPDAADRRGLVRVYDGHGVFVGLAEAEAGKLVPRRLIATVQA
CCCCHHHHHHHHHCCCCCCHHHCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA