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The map label for this gene is pnp

Identifier: 74316711

GI number: 74316711

Start: 732249

End: 734369

Strand: Reverse

Name: pnp

Synonym: Tbd_0693

Alternate gene names: 74316711

Gene position: 734369-732249 (Counterclockwise)

Preceding gene: 74316712

Following gene: 74316708

Centisome position: 25.24

GC content: 66.1

Gene sequence:

>2121_bases
GTGAGCGCTATCAAGAAAACGTTTACCTACGGTCGCCACCAAGTCACGCTGGAAACCGGCGAAATCGCGCGTCAGGCGTC
CGGCGCCGTCGTCGTCAACATGGACGACACGATGGTGCTCGTGACCGTGGTCGCCAAGAACGAAGTCAAGCCCGGCCAGG
ATTTCTTCCCGCTGACCGTCGACTACCAGGAAAAGACCTACGCCGCCGGCCGCATCCCCGGGGGATTTCTCAAGCGCGAA
AGCCGGCCGTCGGAAGGTGAAACGCTGATCTCCCGCCTGATCGACCGCCCGATCCGCCCGCTCTTCCCCGAAGGCTTCTT
CAACGAAGTGCAGATCATCGCGACGGTGATGTCGTCGAATCCCGAAGTCAGCGCCGACATCCCCGCGCTGATCGGCGCTT
CGGCCGCGCTCTCGCTCTCCGGCCTGCCGTTCGACGGCCCGGTCGGCGCGGCGCGCGTCGGCTTCATCAACGGCGAATAC
GTGCTCAACCCGACCAACGCCGAACTCAAGGATTCCGCGCTCGACCTCGTCGTCGCCGGCACCGAAAGCGCCGTGCTGAT
GGTTGAATCCGAGGCCATGGAACTGCCCGAGGACATCATGCTCGGCGCTGTCGTCTTCGGCCACACGCAGATGCAGGCCG
CGATCAACGCGATCAACGAGCTGGCCGACGAAGCCGGCGCCGACGCCTGGGACTGGGCGCCGCCGGCCGAAGACACGGCG
ATGGTCGACCGCCTCAAGGCGCTCGCCGAAGACGGCCTGCAACAGGCCTACAACATCAAGCAGAAGCGGGCACGCATGGC
CGCCGTCGACGAGGTGCGCAGCAAGGCCTTCGCCGAACTCATCACGGCCGACATGGACACGGTGGCGGCCAACCACGTCA
AGGACGCGTTCCACCGCCTCGAAGCCGGCGTCGTGCGCAACCGCATCCTCTCGGGCCAGCCGCGCATCGACGGCCGCGAC
ACCCGCACCGTGCGCCCGATCACGATCCGTACCGGCGTGCTGCCGCGCGCCCACGGCTCGGCGCTGTTCACGCGCGGTGA
AACGCAGGCCCTGGTCGTGACGACGCTCGGCACGGGCCGCGACGAGCAGACCATCGACGCACTCGAGGGCAGCTACTCCG
ACCGCTTCATGCTGCACTACAACATGCCGCCCTATGCGACCGGCGAAACCGGCCGCGTCGGCTCGCCCAAGCGTCGCGAG
ATCGGCCACGGCCGGCTCGCCAAGCGCGCGCTGCTGGCGGTGCTGCCGAGCAAGGAAGAATTCGGCTACACCATGCGCGT
GGTCTCCGAAATCACCGAATCGAACGGCTCGTCGTCGATGGCCTCGGTGTGCGGCGGCTGCCTCTCGCTGATGGACGCCG
GCGCGCCGCTCAAGGCGCACGTCGCCGGCATCGCCATGGGCCTGATCAAGGAAGGCAACCGCTTCGCCGTGCTGACGGAC
ATTCTCGGCGACGAAGACCACCTCGGCGACATGGACTTCAAGGTCGCGGGCACCGAAAACGGCGTGACCGCGCTGCAGAT
GGACATCAAGATCACGGGCATCACCAAGGAGATCATGCAGGCCGCGCTCACCCAGGCGAAGGAAGGCCGCATGCACATCC
TCGGCATCATGAAGGCGTCGGTCTCCGAGACCCATGAGATGTCGGCCTACGCGCCGCGCATCATCGCGATGAAGATCAAT
CCCGAGAAGATCCGCGACGTGATCGGCAAGGGCGGCGCCGTGATCCGCGCGCTGACCGAGGAAACCGGCACCCAGATCGA
CATCCAGGAAGACGGCAGCGTAAAGATCGCCTGTACCAGCATGGAGGCCGGCGAACTCGCGAAGAAGCGCATCGAGGAAA
TCACCGCCGAGGTCGAAGTCGGCAAGGTCTACGAGGGCCCGGTCATCAAGCTGCTCGACTTCGGCGCGATCGTCAACGTG
CTGCCGGGACGCGACGGCCTGCTGCACATCTCGCAGATCGCCCACGAGCGCGTCAATACGATCGGCGACTACCTCAAGGA
AGGCCAGGTCGTGCGCGTGAAGATTCTCGAGGCCGACGAGAAAGGCCGCCTGCGCCTGTCGATGAAGGCCTTGCTCGAAG
CCCCCGCGCCGACGAGCGGCGGCGAAGAACACGCGAGCTGA

Upstream 100 bases:

>100_bases
CGCGCGTCCTGCTGCACCGGCACAGTGGACGGGGCCTGTTGCCCCTTGAATTTCCCACGCCCCGCTTCTGCGGGGCGTTT
TTGCTTTGAAAGGAACTCGT

Downstream 100 bases:

>100_bases
GTGGACTGCGTGCGCCGCAAGCGCACGCCGCGAACAAAAAAAAACGCGCCTCAGGGCGCGTTTTTTTTGGATTCGGCTGG
CGTCAGACCACTTCGCCGAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MSAIKKTFTYGRHQVTLETGEIARQASGAVVVNMDDTMVLVTVVAKNEVKPGQDFFPLTVDYQEKTYAAGRIPGGFLKRE
SRPSEGETLISRLIDRPIRPLFPEGFFNEVQIIATVMSSNPEVSADIPALIGASAALSLSGLPFDGPVGAARVGFINGEY
VLNPTNAELKDSALDLVVAGTESAVLMVESEAMELPEDIMLGAVVFGHTQMQAAINAINELADEAGADAWDWAPPAEDTA
MVDRLKALAEDGLQQAYNIKQKRARMAAVDEVRSKAFAELITADMDTVAANHVKDAFHRLEAGVVRNRILSGQPRIDGRD
TRTVRPITIRTGVLPRAHGSALFTRGETQALVVTTLGTGRDEQTIDALEGSYSDRFMLHYNMPPYATGETGRVGSPKRRE
IGHGRLAKRALLAVLPSKEEFGYTMRVVSEITESNGSSSMASVCGGCLSLMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
ILGDEDHLGDMDFKVAGTENGVTALQMDIKITGITKEIMQAALTQAKEGRMHILGIMKASVSETHEMSAYAPRIIAMKIN
PEKIRDVIGKGGAVIRALTEETGTQIDIQEDGSVKIACTSMEAGELAKKRIEEITAEVEVGKVYEGPVIKLLDFGAIVNV
LPGRDGLLHISQIAHERVNTIGDYLKEGQVVRVKILEADEKGRLRLSMKALLEAPAPTSGGEEHAS

Sequences:

>Translated_706_residues
MSAIKKTFTYGRHQVTLETGEIARQASGAVVVNMDDTMVLVTVVAKNEVKPGQDFFPLTVDYQEKTYAAGRIPGGFLKRE
SRPSEGETLISRLIDRPIRPLFPEGFFNEVQIIATVMSSNPEVSADIPALIGASAALSLSGLPFDGPVGAARVGFINGEY
VLNPTNAELKDSALDLVVAGTESAVLMVESEAMELPEDIMLGAVVFGHTQMQAAINAINELADEAGADAWDWAPPAEDTA
MVDRLKALAEDGLQQAYNIKQKRARMAAVDEVRSKAFAELITADMDTVAANHVKDAFHRLEAGVVRNRILSGQPRIDGRD
TRTVRPITIRTGVLPRAHGSALFTRGETQALVVTTLGTGRDEQTIDALEGSYSDRFMLHYNMPPYATGETGRVGSPKRRE
IGHGRLAKRALLAVLPSKEEFGYTMRVVSEITESNGSSSMASVCGGCLSLMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
ILGDEDHLGDMDFKVAGTENGVTALQMDIKITGITKEIMQAALTQAKEGRMHILGIMKASVSETHEMSAYAPRIIAMKIN
PEKIRDVIGKGGAVIRALTEETGTQIDIQEDGSVKIACTSMEAGELAKKRIEEITAEVEVGKVYEGPVIKLLDFGAIVNV
LPGRDGLLHISQIAHERVNTIGDYLKEGQVVRVKILEADEKGRLRLSMKALLEAPAPTSGGEEHAS
>Mature_705_residues
SAIKKTFTYGRHQVTLETGEIARQASGAVVVNMDDTMVLVTVVAKNEVKPGQDFFPLTVDYQEKTYAAGRIPGGFLKRES
RPSEGETLISRLIDRPIRPLFPEGFFNEVQIIATVMSSNPEVSADIPALIGASAALSLSGLPFDGPVGAARVGFINGEYV
LNPTNAELKDSALDLVVAGTESAVLMVESEAMELPEDIMLGAVVFGHTQMQAAINAINELADEAGADAWDWAPPAEDTAM
VDRLKALAEDGLQQAYNIKQKRARMAAVDEVRSKAFAELITADMDTVAANHVKDAFHRLEAGVVRNRILSGQPRIDGRDT
RTVRPITIRTGVLPRAHGSALFTRGETQALVVTTLGTGRDEQTIDALEGSYSDRFMLHYNMPPYATGETGRVGSPKRREI
GHGRLAKRALLAVLPSKEEFGYTMRVVSEITESNGSSSMASVCGGCLSLMDAGAPLKAHVAGIAMGLIKEGNRFAVLTDI
LGDEDHLGDMDFKVAGTENGVTALQMDIKITGITKEIMQAALTQAKEGRMHILGIMKASVSETHEMSAYAPRIIAMKINP
EKIRDVIGKGGAVIRALTEETGTQIDIQEDGSVKIACTSMEAGELAKKRIEEITAEVEVGKVYEGPVIKLLDFGAIVNVL
PGRDGLLHISQIAHERVNTIGDYLKEGQVVRVKILEADEKGRLRLSMKALLEAPAPTSGGEEHAS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=717, Percent_Identity=37.9358437935844, Blast_Score=430, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=64.8414985590778, Blast_Score=921, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=714, Percent_Identity=32.7731092436975, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI281362905, Length=720, Percent_Identity=37.6388888888889, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=720, Percent_Identity=37.6388888888889, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=720, Percent_Identity=37.6388888888889, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.8340943683409, Blast_Score=387, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_THIDA (Q3SKX5)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314451.1
- HSSP:   Q9UXC2
- ProteinModelPortal:   Q3SKX5
- STRING:   Q3SKX5
- GeneID:   3672661
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0693
- NMPDR:   fig|292415.3.peg.722
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q3SKX5
- ProtClustDB:   PRK11824
- BioCyc:   TDEN292415:TBD_0693-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75877; Mature: 75746

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIKKTFTYGRHQVTLETGEIARQASGAVVVNMDDTMVLVTVVAKNEVKPGQDFFPLTV
CCCHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCEEE
DYQEKTYAAGRIPGGFLKRESRPSEGETLISRLIDRPIRPLFPEGFFNEVQIIATVMSSN
ECCCCEEECCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
PEVSADIPALIGASAALSLSGLPFDGPVGAARVGFINGEYVLNPTNAELKDSALDLVVAG
CCCCCCCHHHHCCCHHEEECCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEEC
TESAVLMVESEAMELPEDIMLGAVVFGHTQMQAAINAINELADEAGADAWDWAPPAEDTA
CCCEEEEEECHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MVDRLKALAEDGLQQAYNIKQKRARMAAVDEVRSKAFAELITADMDTVAANHVKDAFHRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGVVRNRILSGQPRIDGRDTRTVRPITIRTGVLPRAHGSALFTRGETQALVVTTLGTGR
HHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCC
DEQTIDALEGSYSDRFMLHYNMPPYATGETGRVGSPKRREIGHGRLAKRALLAVLPSKEE
CCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCHH
FGYTMRVVSEITESNGSSSMASVCGGCLSLMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEE
ILGDEDHLGDMDFKVAGTENGVTALQMDIKITGITKEIMQAALTQAKEGRMHILGIMKAS
CCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHH
VSETHEMSAYAPRIIAMKINPEKIRDVIGKGGAVIRALTEETGTQIDIQEDGSVKIACTS
HHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCEEEEEHHCCCCEEEEECCCCEEEEEEC
MEAGELAKKRIEEITAEVEVGKVYEGPVIKLLDFGAIVNVLPGRDGLLHISQIAHERVNT
CCHHHHHHHHHHHHHHHEEECEECCCCEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IGDYLKEGQVVRVKILEADEKGRLRLSMKALLEAPAPTSGGEEHAS
HHHHHHCCCEEEEEEEECCCCCCEEEEHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAIKKTFTYGRHQVTLETGEIARQASGAVVVNMDDTMVLVTVVAKNEVKPGQDFFPLTV
CCHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCEEE
DYQEKTYAAGRIPGGFLKRESRPSEGETLISRLIDRPIRPLFPEGFFNEVQIIATVMSSN
ECCCCEEECCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
PEVSADIPALIGASAALSLSGLPFDGPVGAARVGFINGEYVLNPTNAELKDSALDLVVAG
CCCCCCCHHHHCCCHHEEECCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEEC
TESAVLMVESEAMELPEDIMLGAVVFGHTQMQAAINAINELADEAGADAWDWAPPAEDTA
CCCEEEEEECHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MVDRLKALAEDGLQQAYNIKQKRARMAAVDEVRSKAFAELITADMDTVAANHVKDAFHRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGVVRNRILSGQPRIDGRDTRTVRPITIRTGVLPRAHGSALFTRGETQALVVTTLGTGR
HHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCC
DEQTIDALEGSYSDRFMLHYNMPPYATGETGRVGSPKRREIGHGRLAKRALLAVLPSKEE
CCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCHH
FGYTMRVVSEITESNGSSSMASVCGGCLSLMDAGAPLKAHVAGIAMGLIKEGNRFAVLTD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEE
ILGDEDHLGDMDFKVAGTENGVTALQMDIKITGITKEIMQAALTQAKEGRMHILGIMKAS
CCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHH
VSETHEMSAYAPRIIAMKINPEKIRDVIGKGGAVIRALTEETGTQIDIQEDGSVKIACTS
HHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCEEEEEHHCCCCEEEEECCCCEEEEEEC
MEAGELAKKRIEEITAEVEVGKVYEGPVIKLLDFGAIVNVLPGRDGLLHISQIAHERVNT
CCHHHHHHHHHHHHHHHEEECEECCCCEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IGDYLKEGQVVRVKILEADEKGRLRLSMKALLEAPAPTSGGEEHAS
HHHHHHCCCEEEEEEEECCCCCCEEEEHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA