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The map label for this gene is vapI [H]

Identifier: 74316690

GI number: 74316690

Start: 713650

End: 714165

Strand: Reverse

Name: vapI [H]

Synonym: Tbd_0672

Alternate gene names: 74316690

Gene position: 714165-713650 (Counterclockwise)

Preceding gene: 74316691

Following gene: 74316687

Centisome position: 24.54

GC content: 64.73

Gene sequence:

>516_bases
ATGGCCATCATGAACGGCATGCGCCCGATCCACCCGGGCGAAATCCTCCGCGAGGAATTCCTGATTCCGCTGGGGCTCTC
GGCCAACGCGCTGGCCAACGCCCTGCGCGTCCCCGCACCGCGCATCAACGACATCGCGCGCGAACGCCGCGCCGTGTCTG
CAGACACCGCTCTGCGGCTGGCGCGCTACTTCGGCACGAGTATCGAATTCTGGTTAGGTCTGCAGTCGGACTACGACATG
AAAATTGCCCTGGCCGAGCATGGCGCGCAGATCGAGCGCGATGTCATGCGATGGAACAGGCCGCATGAAGAATCCGCAGG
AGGAAGCGTGATGGCCTCAGTATCAGAAGATCACTTGCTCCCCGCCACCCAGACCCCCTTCCACCCGGCCACCGGCGGTG
CTTTCCTGAACGCCTCGACCCGCGCCAGCATCACCGCACTGGGCCGATCGCCCGGCGCCTTCGCCAGACATTCCCTGAAC
GCCGCTTCAGCCGCCGTCCAGTCTTCCGCACGATAA

Upstream 100 bases:

>100_bases
GAGCATACGCATCAACGATCAATGGCGAATCTGCTTCCGGTTCGAGAACGGCAACGCGTACGACGTGGAAATCGTCGATT
ACCACTGACAGGAGACGACA

Downstream 100 bases:

>100_bases
CGAGCGAGCCCGGCTTCGTACGCCGTGACGGCCAGCCGGCTCGCTTCGCTCAATTCGTTCGCCATGCCGAGCAATTCGTA
CACACGCACGGGCTGCAGCT

Product: plasmid maintenance system antidote protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAIMNGMRPIHPGEILREEFLIPLGLSANALANALRVPAPRINDIARERRAVSADTALRLARYFGTSIEFWLGLQSDYDM
KIALAEHGAQIERDVMRWNRPHEESAGGSVMASVSEDHLLPATQTPFHPATGGAFLNASTRASITALGRSPGAFARHSLN
AASAAVQSSAR

Sequences:

>Translated_171_residues
MAIMNGMRPIHPGEILREEFLIPLGLSANALANALRVPAPRINDIARERRAVSADTALRLARYFGTSIEFWLGLQSDYDM
KIALAEHGAQIERDVMRWNRPHEESAGGSVMASVSEDHLLPATQTPFHPATGGAFLNASTRASITALGRSPGAFARHSLN
AASAAVQSSAR
>Mature_170_residues
AIMNGMRPIHPGEILREEFLIPLGLSANALANALRVPAPRINDIARERRAVSADTALRLARYFGTSIEFWLGLQSDYDMK
IALAEHGAQIERDVMRWNRPHEESAGGSVMASVSEDHLLPATQTPFHPATGGAFLNASTRASITALGRSPGAFARHSLNA
ASAAVQSSAR

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 18398; Mature: 18267

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIMNGMRPIHPGEILREEFLIPLGLSANALANALRVPAPRINDIARERRAVSADTALRL
CCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARYFGTSIEFWLGLQSDYDMKIALAEHGAQIERDVMRWNRPHEESAGGSVMASVSEDHLL
HHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCC
PATQTPFHPATGGAFLNASTRASITALGRSPGAFARHSLNAASAAVQSSAR
CCCCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIMNGMRPIHPGEILREEFLIPLGLSANALANALRVPAPRINDIARERRAVSADTALRL
CCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARYFGTSIEFWLGLQSDYDMKIALAEHGAQIERDVMRWNRPHEESAGGSVMASVSEDHLL
HHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCC
PATQTPFHPATGGAFLNASTRASITALGRSPGAFARHSLNAASAAVQSSAR
CCCCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]