Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 74316680

GI number: 74316680

Start: 702339

End: 704000

Strand: Reverse

Name: ilvB [H]

Synonym: Tbd_0662

Alternate gene names: 74316680

Gene position: 704000-702339 (Counterclockwise)

Preceding gene: 74316681

Following gene: 74316679

Centisome position: 24.19

GC content: 67.09

Gene sequence:

>1662_bases
ATGAACATTTCCGTCAGCGAACTGATCGTCCGTTACCTCGAACGCCTCGGCATCGGTCACGTCTTCGGCATGCCCGGTGC
GCACATCCTGCCGATCTACGACCGCCTGCGCGACGCCTCGCTGCGCACCGTGCTCGTCAAGCACGAGCAGGGTGCGGCCT
TCATGGCCGGCGGCGAGGCGCGGGTCGCGCGCCGCCCCTCGGCCTGCATCGCGACCTCCGGACCCGGCGCGACCAATCTC
ATCACCGGCATCGCCAACGCCTACGCCGAGCGGCTGCCCGTGCTCGCGATCACCGGCGAGACGTCGACCTACATCTTCGG
CCGCGGCGGCCTGCAGGAAAGCTCGGGAGAAGGCGGCGCGATCGATCAGGGCGCGCTCTTCGCGAGCATCACGCGCTACC
ACAAGCGCATCGAGCGCACCGACTATCTCGGCCAGGCGCTCAACCAGGCGACCCAGGCCCTGCTCGGCCGCGAGCCCGGA
CCGGTGCTGTTGTCGATCCCCTACAACGTGCAGAAGGAATCGGTCGACGCCGGCGCGCTCGAGCAGGTTCACTTCCCGCG
CGCCGAAGTCACGCGCCACACCGCCGCGGTGAGCGAACTCGCCGCGCTCATCGCCGACGCCCGGGCGCCCGTCATCGTCG
CGGGATACGGCTGCATCGCCGCCGGCGCGCGCGAACTCGTCGGCACGCTGTCGGAAAAATTCCGCATCCCGGTGACGAGC
AGTCTCAAGGCCAAGGGCGTCGTCACCGAAGGTTCCGACCTGTCGCTCGGCTGCCTCGGCGTGACGTCCAACGGCGACGC
CTACCGCTACCTCGCCGAGCACGCCGACCTGCTCGTCTTTCTGGGCGCGAGCTTCAACGAACGCACGAGCTATCTGTGGG
ACGCGGAACTCCTGGCCCGCAAGAAGATCGCCCAGGTCGACCGCGACCCCGCGCAGCTCGGACGCGTCTTCCAGCCCGAT
GTCGCGGTGTGCGGCGATATCCGTGCCGTCGTCGAAGACCTGCTCGCCGCACTCGCAACGAGCGGCGCCGCGCCGAAGAA
GACCGAACGTCTCGCGCGTCATCGGGCCGGACTCAAGCGACCGACGCAGGAGGCCGCGCCCGAGAACGAAGGGTTTCAGC
TGATCCGCCACTTCTTCCACCTGCTCGCGAAGCGGTTTCCACGCGAAGCGCTCGTGTTCGACGACAACATCGTCTTCGCC
CAGAGCTACTTCGACGTGTCGGACCGCAACCGCTATTTCCCCAACACCGGCATCTCGTCGCTCGGTCACGCGCTGCCTGC
CGCAATCGGGGCGCGCTGCCACGCCAAGGACAGCCCGGCCTTCGCGATCCTCGGCGACGGCGGCTTTCAGATGTGCTGCA
TGGAAATGATGACGGCGGTGAACTACGGCATCCCGCTCAACGTCGTCGTGATCAACAACGGCACGCTCTCGCTGATCCGC
AAGAACCAGTTCCAGCTCTACGGTGAGCGCTACATCGACTGCGATTTCACCAATCCCGACTTCGCCCTGCTCGCCCGCTC
CTTCGGCGTCGCGCACTATCGCGTCGCCAACGAAGCCGAACTCGACGCCGTCTTCGCCGACGCCGACCTCATCGGCGGGA
TCAACCTCATCGAAATCCTGCTCGACAAGAACGCCTTCCCGCGCTACCTGGCAGGCCGGTAG

Upstream 100 bases:

>100_bases
CGGTCTTGCGCCACGCCGAGGTTTTCCTAAGCCGCCGCTTGTGCTCCAATCCGGAGGCCGTCCCAGCAGACGCGCGCTGC
GACAGCGCACGACAAGACTC

Downstream 100 bases:

>100_bases
CGGCAACCCGAGACCGTCACCCGATGCCGAGCTTCCCCATGACTCCCCGAGCCCTCCGATCCGCGCCGCAACTGCGTTCG
CTCGCCCGCATGCTCGCTGC

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MNISVSELIVRYLERLGIGHVFGMPGAHILPIYDRLRDASLRTVLVKHEQGAAFMAGGEARVARRPSACIATSGPGATNL
ITGIANAYAERLPVLAITGETSTYIFGRGGLQESSGEGGAIDQGALFASITRYHKRIERTDYLGQALNQATQALLGREPG
PVLLSIPYNVQKESVDAGALEQVHFPRAEVTRHTAAVSELAALIADARAPVIVAGYGCIAAGARELVGTLSEKFRIPVTS
SLKAKGVVTEGSDLSLGCLGVTSNGDAYRYLAEHADLLVFLGASFNERTSYLWDAELLARKKIAQVDRDPAQLGRVFQPD
VAVCGDIRAVVEDLLAALATSGAAPKKTERLARHRAGLKRPTQEAAPENEGFQLIRHFFHLLAKRFPREALVFDDNIVFA
QSYFDVSDRNRYFPNTGISSLGHALPAAIGARCHAKDSPAFAILGDGGFQMCCMEMMTAVNYGIPLNVVVINNGTLSLIR
KNQFQLYGERYIDCDFTNPDFALLARSFGVAHYRVANEAELDAVFADADLIGGINLIEILLDKNAFPRYLAGR

Sequences:

>Translated_553_residues
MNISVSELIVRYLERLGIGHVFGMPGAHILPIYDRLRDASLRTVLVKHEQGAAFMAGGEARVARRPSACIATSGPGATNL
ITGIANAYAERLPVLAITGETSTYIFGRGGLQESSGEGGAIDQGALFASITRYHKRIERTDYLGQALNQATQALLGREPG
PVLLSIPYNVQKESVDAGALEQVHFPRAEVTRHTAAVSELAALIADARAPVIVAGYGCIAAGARELVGTLSEKFRIPVTS
SLKAKGVVTEGSDLSLGCLGVTSNGDAYRYLAEHADLLVFLGASFNERTSYLWDAELLARKKIAQVDRDPAQLGRVFQPD
VAVCGDIRAVVEDLLAALATSGAAPKKTERLARHRAGLKRPTQEAAPENEGFQLIRHFFHLLAKRFPREALVFDDNIVFA
QSYFDVSDRNRYFPNTGISSLGHALPAAIGARCHAKDSPAFAILGDGGFQMCCMEMMTAVNYGIPLNVVVINNGTLSLIR
KNQFQLYGERYIDCDFTNPDFALLARSFGVAHYRVANEAELDAVFADADLIGGINLIEILLDKNAFPRYLAGR
>Mature_553_residues
MNISVSELIVRYLERLGIGHVFGMPGAHILPIYDRLRDASLRTVLVKHEQGAAFMAGGEARVARRPSACIATSGPGATNL
ITGIANAYAERLPVLAITGETSTYIFGRGGLQESSGEGGAIDQGALFASITRYHKRIERTDYLGQALNQATQALLGREPG
PVLLSIPYNVQKESVDAGALEQVHFPRAEVTRHTAAVSELAALIADARAPVIVAGYGCIAAGARELVGTLSEKFRIPVTS
SLKAKGVVTEGSDLSLGCLGVTSNGDAYRYLAEHADLLVFLGASFNERTSYLWDAELLARKKIAQVDRDPAQLGRVFQPD
VAVCGDIRAVVEDLLAALATSGAAPKKTERLARHRAGLKRPTQEAAPENEGFQLIRHFFHLLAKRFPREALVFDDNIVFA
QSYFDVSDRNRYFPNTGISSLGHALPAAIGARCHAKDSPAFAILGDGGFQMCCMEMMTAVNYGIPLNVVVINNGTLSLIR
KNQFQLYGERYIDCDFTNPDFALLARSFGVAHYRVANEAELDAVFADADLIGGINLIEILLDKNAFPRYLAGR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=490, Percent_Identity=24.2857142857143, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI21361361, Length=581, Percent_Identity=24.0963855421687, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=30.550621669627, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87081685, Length=547, Percent_Identity=27.6051188299817, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1786717, Length=524, Percent_Identity=27.6717557251908, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=26.2118491921005, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788716, Length=489, Percent_Identity=24.9488752556237, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17542570, Length=550, Percent_Identity=24.1818181818182, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=21.830985915493, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=523, Percent_Identity=22.1797323135755, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=28.2415630550622, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320123, Length=584, Percent_Identity=22.2602739726027, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320816, Length=560, Percent_Identity=23.75, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=584, Percent_Identity=22.7739726027397, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=584, Percent_Identity=22.9452054794521, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=24.435318275154, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59720; Mature: 59720

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISVSELIVRYLERLGIGHVFGMPGAHILPIYDRLRDASLRTVLVKHEQGAAFMAGGEA
CCCCHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHHCCCCEEEEEEEECCCCEEEECCCH
RVARRPSACIATSGPGATNLITGIANAYAERLPVLAITGETSTYIFGRGGLQESSGEGGA
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCC
IDQGALFASITRYHKRIERTDYLGQALNQATQALLGREPGPVLLSIPYNVQKESVDAGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCH
EQVHFPRAEVTRHTAAVSELAALIADARAPVIVAGYGCIAAGARELVGTLSEKFRIPVTS
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHEECCCCC
SLKAKGVVTEGSDLSLGCLGVTSNGDAYRYLAEHADLLVFLGASFNERTSYLWDAELLAR
CCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHCCCEEEEECCCCCCCCCEEECHHHHHH
KKIAQVDRDPAQLGRVFQPDVAVCGDIRAVVEDLLAALATSGAAPKKTERLARHRAGLKR
HHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
PTQEAAPENEGFQLIRHFFHLLAKRFPREALVFDDNIVFAQSYFDVSDRNRYFPNTGISS
CCHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCHHH
LGHALPAAIGARCHAKDSPAFAILGDGGFQMCCMEMMTAVNYGIPLNVVVINNGTLSLIR
HHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
KNQFQLYGERYIDCDFTNPDFALLARSFGVAHYRVANEAELDAVFADADLIGGINLIEIL
CCCCEEECCEEEEECCCCCHHHHHHHHHCCEEEEECCCCCHHEEEECHHHHCCHHHHHHH
LDKNAFPRYLAGR
HCCCCCCHHHCCC
>Mature Secondary Structure
MNISVSELIVRYLERLGIGHVFGMPGAHILPIYDRLRDASLRTVLVKHEQGAAFMAGGEA
CCCCHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHHCCCCEEEEEEEECCCCEEEECCCH
RVARRPSACIATSGPGATNLITGIANAYAERLPVLAITGETSTYIFGRGGLQESSGEGGA
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCC
IDQGALFASITRYHKRIERTDYLGQALNQATQALLGREPGPVLLSIPYNVQKESVDAGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCH
EQVHFPRAEVTRHTAAVSELAALIADARAPVIVAGYGCIAAGARELVGTLSEKFRIPVTS
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHEECCCCC
SLKAKGVVTEGSDLSLGCLGVTSNGDAYRYLAEHADLLVFLGASFNERTSYLWDAELLAR
CCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHCCCEEEEECCCCCCCCCEEECHHHHHH
KKIAQVDRDPAQLGRVFQPDVAVCGDIRAVVEDLLAALATSGAAPKKTERLARHRAGLKR
HHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
PTQEAAPENEGFQLIRHFFHLLAKRFPREALVFDDNIVFAQSYFDVSDRNRYFPNTGISS
CCHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCHHH
LGHALPAAIGARCHAKDSPAFAILGDGGFQMCCMEMMTAVNYGIPLNVVVINNGTLSLIR
HHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
KNQFQLYGERYIDCDFTNPDFALLARSFGVAHYRVANEAELDAVFADADLIGGINLIEIL
CCCCEEECCEEEEECCCCCHHHHHHHHHCCEEEEECCCCCHHEEEECHHHHCCHHHHHHH
LDKNAFPRYLAGR
HCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]