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The map label for this gene is pyrD

Identifier: 74316663

GI number: 74316663

Start: 685315

End: 686328

Strand: Reverse

Name: pyrD

Synonym: Tbd_0645

Alternate gene names: 74316663

Gene position: 686328-685315 (Counterclockwise)

Preceding gene: 74316664

Following gene: 74316662

Centisome position: 23.59

GC content: 67.95

Gene sequence:

>1014_bases
ATGCTTTATTCGCTGATCCGCCCCGCCCTCTTTTCGCTCGACGCCGAAGACGCCCACGGCCTGACGCTCACCGGCCTCGA
CGTGGCACAGCGCCTCGGCCTTGTCGGTCTGCAGCCGCGAGCCACCGGCAAACCGGTGCAGGTCATGGGAATCGACTTTC
CCAATGCGGTCGGTCTCGCGGCGGGGCTGGACAAGGACGGCGCCCATCTGAAGGGCCTCGCGGCGCTCGGATTCGGCTTT
CTCGAAATCGGCACGGTCACCCCGCGTCCGCAGCCCGGGAATCCGAAACCGCGGCTCTTCCGGCTCCCCGCGGCCGAGGG
CATCATCAACCGCATGGGCTTCAACAACCTCGGCGTCGACAACCTCGTGCGCAACGTCGTCGCGAGTGGCTACACCGGCG
TGCTCGGCATCAACATCGGCAAGAACAAGGACACGCCGAACGAGCGCGCGGCCGACGACTATCTCGCCTGCCTCGACAAG
GTCTACGCGCACGCGCGCTACGTCACCGTCAACATCTCGTCGCCAAACACGCAGAACCTGCGTGAGCTTCAGCAGGACGA
GGCGCTCGATGCACTGCTCTCGGCGATCAAGCTTCGCCAGTCCGAGCTCGCGCAGCAGCATGGCCGCTACGTGCCGATCG
CGCTGAAAATCGCGCCCGACCTCGACGAGGCGCAGATCGCCGCAATCGCCGCGCTGCTGATGCGCCACGGCATCGACGCC
GTGATCGCGACCAACACGACGATCGCACGCGACGCCGTCGCCGGACTGCCAAACGCGAACGAGAGCGGCGGCCTCTCCGG
CGCGCCGGTGCGCGAGGCCTCGACCCGCGTCGTGCGCACGCTCGCGCAACACCTCGGCGGTGCGCTGCCGATCATCGGCG
TCGGCGGCATCCTCTCCGGCGACGACGCGCGCGCCAAGATCGCCGCCGGCGCCTCGCTCGTGCAACTCTATTCGGGACTG
ATCTATCGCGGTCCGGGGCTCGTGCGCGAATGCGTCGAAAGGCTCGCGCAGTGA

Upstream 100 bases:

>100_bases
AGAGCGAGCGCACGCTCGACCGTGCAATATTCGACGACCCGGCCGCGGGTTCGGTCACCGCCGCGACCCGCCGTCCCTCT
CCTTCCGCCCCCGCCTGAAC

Downstream 100 bases:

>100_bases
TCGTCCGCCTGCTCGCCGGCGTCGCGGCGCTGCTGCTGCACGCGGCGGCCCTCGCGATGCCGCCGCAGCCCATCATCACG
CATGTCGACCAGCCGGCGCT

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLYSLIRPALFSLDAEDAHGLTLTGLDVAQRLGLVGLQPRATGKPVQVMGIDFPNAVGLAAGLDKDGAHLKGLAALGFGF
LEIGTVTPRPQPGNPKPRLFRLPAAEGIINRMGFNNLGVDNLVRNVVASGYTGVLGINIGKNKDTPNERAADDYLACLDK
VYAHARYVTVNISSPNTQNLRELQQDEALDALLSAIKLRQSELAQQHGRYVPIALKIAPDLDEAQIAAIAALLMRHGIDA
VIATNTTIARDAVAGLPNANESGGLSGAPVREASTRVVRTLAQHLGGALPIIGVGGILSGDDARAKIAAGASLVQLYSGL
IYRGPGLVRECVERLAQ

Sequences:

>Translated_337_residues
MLYSLIRPALFSLDAEDAHGLTLTGLDVAQRLGLVGLQPRATGKPVQVMGIDFPNAVGLAAGLDKDGAHLKGLAALGFGF
LEIGTVTPRPQPGNPKPRLFRLPAAEGIINRMGFNNLGVDNLVRNVVASGYTGVLGINIGKNKDTPNERAADDYLACLDK
VYAHARYVTVNISSPNTQNLRELQQDEALDALLSAIKLRQSELAQQHGRYVPIALKIAPDLDEAQIAAIAALLMRHGIDA
VIATNTTIARDAVAGLPNANESGGLSGAPVREASTRVVRTLAQHLGGALPIIGVGGILSGDDARAKIAAGASLVQLYSGL
IYRGPGLVRECVERLAQ
>Mature_337_residues
MLYSLIRPALFSLDAEDAHGLTLTGLDVAQRLGLVGLQPRATGKPVQVMGIDFPNAVGLAAGLDKDGAHLKGLAALGFGF
LEIGTVTPRPQPGNPKPRLFRLPAAEGIINRMGFNNLGVDNLVRNVVASGYTGVLGINIGKNKDTPNERAADDYLACLDK
VYAHARYVTVNISSPNTQNLRELQQDEALDALLSAIKLRQSELAQQHGRYVPIALKIAPDLDEAQIAAIAALLMRHGIDA
VIATNTTIARDAVAGLPNANESGGLSGAPVREASTRVVRTLAQHLGGALPIIGVGGILSGDDARAKIAAGASLVQLYSGL
IYRGPGLVRECVERLAQ

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=331, Percent_Identity=43.8066465256798, Blast_Score=229, Evalue=3e-60,
Organism=Escherichia coli, GI1787177, Length=336, Percent_Identity=58.6309523809524, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17509475, Length=347, Percent_Identity=42.6512968299712, Blast_Score=233, Evalue=7e-62,
Organism=Drosophila melanogaster, GI281361352, Length=342, Percent_Identity=38.8888888888889, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17137316, Length=342, Percent_Identity=38.8888888888889, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_THIDA (Q3SL21)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314403.1
- ProteinModelPortal:   Q3SL21
- SMR:   Q3SL21
- STRING:   Q3SL21
- GeneID:   3672610
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0645
- NMPDR:   fig|292415.3.peg.574
- eggNOG:   COG0167
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- PhylomeDB:   Q3SL21
- ProtClustDB:   PRK05286
- BioCyc:   TDEN292415:TBD_0645-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 35358; Mature: 35358

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSLIRPALFSLDAEDAHGLTLTGLDVAQRLGLVGLQPRATGKPVQVMGIDFPNAVGLA
CCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHH
AGLDKDGAHLKGLAALGFGFLEIGTVTPRPQPGNPKPRLFRLPAAEGIINRMGFNNLGVD
CCCCCCCCHHHHHHHHCCCHHEECCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCHH
NLVRNVVASGYTGVLGINIGKNKDTPNERAADDYLACLDKVYAHARYVTVNISSPNTQNL
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHH
RELQQDEALDALLSAIKLRQSELAQQHGRYVPIALKIAPDLDEAQIAAIAALLMRHGIDA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
VIATNTTIARDAVAGLPNANESGGLSGAPVREASTRVVRTLAQHLGGALPIIGVGGILSG
EEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCEECC
DDARAKIAAGASLVQLYSGLIYRGPGLVRECVERLAQ
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLYSLIRPALFSLDAEDAHGLTLTGLDVAQRLGLVGLQPRATGKPVQVMGIDFPNAVGLA
CCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHH
AGLDKDGAHLKGLAALGFGFLEIGTVTPRPQPGNPKPRLFRLPAAEGIINRMGFNNLGVD
CCCCCCCCHHHHHHHHCCCHHEECCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCHH
NLVRNVVASGYTGVLGINIGKNKDTPNERAADDYLACLDKVYAHARYVTVNISSPNTQNL
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHH
RELQQDEALDALLSAIKLRQSELAQQHGRYVPIALKIAPDLDEAQIAAIAALLMRHGIDA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
VIATNTTIARDAVAGLPNANESGGLSGAPVREASTRVVRTLAQHLGGALPIIGVGGILSG
EEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCEECC
DDARAKIAAGASLVQLYSGLIYRGPGLVRECVERLAQ
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA