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The map label for this gene is ald [H]

Identifier: 74316662

GI number: 74316662

Start: 682954

End: 684036

Strand: Reverse

Name: ald [H]

Synonym: Tbd_0644

Alternate gene names: 74316662

Gene position: 684036-682954 (Counterclockwise)

Preceding gene: 74316663

Following gene: 74316661

Centisome position: 23.51

GC content: 71.47

Gene sequence:

>1083_bases
ATGCACATAGGCATCCCGAAGGAAATCAAGGACCACGAATACCGCGTCGCGCTCACCCCCGAAGGCGCGCGTTCGCTCGT
CGATGCCGGCCATGCGGTGAGCGTCGAGAGCCACGCCGGCGCCGCGGTCGGCTTCGGCGACGACGCCTACCGCGCCGCGG
GTGCGACCGTCGTGCCGGATGCAGCCGGCGTGTGGGCGGCCGAGCTCGTCGTCAAGGTCAAGGAACCGCAGCCGCAAGAG
ACACAGCGGCTCCGGGCGGGGCAGCTGCTTTTCTGCTATCTGCACCTCGCGGCAGCGCCCTCGCTCGCGCGCGAACTCAT
GACGCGCGGCGTCAGCGCGATCGCCTACGAAACCGTCGTCGGCCCCGCGGGCGGCCTGCCGCTGTTGCAGCCGATGTCCG
ACATCGCGGGCCGGCTCGCCCCGCAGATGGGCGCGCTCGGGCTGCACAGCTCGCACGGCGGCAACGGCAAGCTGATCAGC
GGCCTGCCCGGCGTCGCGCCGGCCCACGTCCTCGTCATCGGCGCCGGCGTCGTCGGCATGGCAGCGGTGCGCACAGCGAT
CGGCCTCGGTGCACGCGTCACCCTCATCGACCGCCTGCGCGACAAGCTCGCGCAGGCCGAAACCCTGTTCGGCGCGCGCG
TCGAGACGCGCTTTTCGTCGCCCGACGCGATCGCCGAATGCGTCGAGCAGGCCGACATCGTGATCGGCGCGGCGCAGATC
CCGGGGCGCCACGCGCCCCGCCTGATCAGCCTCGACCTGCTGCGCCGCATGGGCGCCGGCAGCGTGCTCGTCGACGTCGC
GATCGACCAGGGCGGCATCGCCGAAACCTCGCGCCCGACCACCCACAGCGACCCTTTCTTCACGGCCGAAGGCGTCGTCC
ATTACTGCGTCACCAACATGCCCGGTGCCGTCGCCCGCACCGCAACCGAAGCGCTGACCCACGCGACGCTGCCCTACGTT
GAGGCACTCGCCGCGCAAGGCCTCGAAGCGCTCGCCGCCGACCCCGGACTGCACGCCGGGCTGCACGTCCACGCCGGCAA
GATCATGCACGCCGGGCTCGCACTCGATCTCAACTTCGACTGA

Upstream 100 bases:

>100_bases
CGGCTCAGCCGGATGGCACCCGCGTATTACCGCGTCGAACGCGCCGGGCCGCGCCGCGCGTCCCGTAGTCGAGCCGCAAA
CCGAGAGCCAGGAGGACCCC

Downstream 100 bases:

>100_bases
GCGGCCGGCCAAGGCTTCGAATTCTTTCCCCCGCCCGGGGAAGCCCCGGCAGTGCGTCGGCCGGACTTGCCGGTCCGACG
GGGCGCCTCGTTCCACGCTT

Product: L-alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MHIGIPKEIKDHEYRVALTPEGARSLVDAGHAVSVESHAGAAVGFGDDAYRAAGATVVPDAAGVWAAELVVKVKEPQPQE
TQRLRAGQLLFCYLHLAAAPSLARELMTRGVSAIAYETVVGPAGGLPLLQPMSDIAGRLAPQMGALGLHSSHGGNGKLIS
GLPGVAPAHVLVIGAGVVGMAAVRTAIGLGARVTLIDRLRDKLAQAETLFGARVETRFSSPDAIAECVEQADIVIGAAQI
PGRHAPRLISLDLLRRMGAGSVLVDVAIDQGGIAETSRPTTHSDPFFTAEGVVHYCVTNMPGAVARTATEALTHATLPYV
EALAAQGLEALAADPGLHAGLHVHAGKIMHAGLALDLNFD

Sequences:

>Translated_360_residues
MHIGIPKEIKDHEYRVALTPEGARSLVDAGHAVSVESHAGAAVGFGDDAYRAAGATVVPDAAGVWAAELVVKVKEPQPQE
TQRLRAGQLLFCYLHLAAAPSLARELMTRGVSAIAYETVVGPAGGLPLLQPMSDIAGRLAPQMGALGLHSSHGGNGKLIS
GLPGVAPAHVLVIGAGVVGMAAVRTAIGLGARVTLIDRLRDKLAQAETLFGARVETRFSSPDAIAECVEQADIVIGAAQI
PGRHAPRLISLDLLRRMGAGSVLVDVAIDQGGIAETSRPTTHSDPFFTAEGVVHYCVTNMPGAVARTATEALTHATLPYV
EALAAQGLEALAADPGLHAGLHVHAGKIMHAGLALDLNFD
>Mature_360_residues
MHIGIPKEIKDHEYRVALTPEGARSLVDAGHAVSVESHAGAAVGFGDDAYRAAGATVVPDAAGVWAAELVVKVKEPQPQE
TQRLRAGQLLFCYLHLAAAPSLARELMTRGVSAIAYETVVGPAGGLPLLQPMSDIAGRLAPQMGALGLHSSHGGNGKLIS
GLPGVAPAHVLVIGAGVVGMAAVRTAIGLGARVTLIDRLRDKLAQAETLFGARVETRFSSPDAIAECVEQADIVIGAAQI
PGRHAPRLISLDLLRRMGAGSVLVDVAIDQGGIAETSRPTTHSDPFFTAEGVVHYCVTNMPGAVARTATEALTHATLPYV
EALAAQGLEALAADPGLHAGLHVHAGKIMHAGLALDLNFD

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=341, Percent_Identity=32.5513196480938, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI122939153, Length=341, Percent_Identity=32.5513196480938, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1787887, Length=324, Percent_Identity=33.9506172839506, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17550456, Length=337, Percent_Identity=29.080118694362, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 37115; Mature: 37115

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGIPKEIKDHEYRVALTPEGARSLVDAGHAVSVESHAGAAVGFGDDAYRAAGATVVPD
CCCCCCCCCCCCCEEEEECCCHHHHHHCCCCEEEECCCCCEEECCCCHHHHHCCCEECCC
AAGVWAAELVVKVKEPQPQETQRLRAGQLLFCYLHLAAAPSLARELMTRGVSAIAYETVV
CCCHHHHHHEEEECCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHC
GPAGGLPLLQPMSDIAGRLAPQMGALGLHSSHGGNGKLISGLPGVAPAHVLVIGAGVVGM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCHHHH
AAVRTAIGLGARVTLIDRLRDKLAQAETLFGARVETRFSSPDAIAECVEQADIVIGAAQI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHEEEEECCC
PGRHAPRLISLDLLRRMGAGSVLVDVAIDQGGIAETSRPTTHSDPFFTAEGVVHYCVTNM
CCCCCCCEEEHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCC
PGAVARTATEALTHATLPYVEALAAQGLEALAADPGLHAGLHVHAGKIMHAGLALDLNFD
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCEEECCEEEEECCC
>Mature Secondary Structure
MHIGIPKEIKDHEYRVALTPEGARSLVDAGHAVSVESHAGAAVGFGDDAYRAAGATVVPD
CCCCCCCCCCCCCEEEEECCCHHHHHHCCCCEEEECCCCCEEECCCCHHHHHCCCEECCC
AAGVWAAELVVKVKEPQPQETQRLRAGQLLFCYLHLAAAPSLARELMTRGVSAIAYETVV
CCCHHHHHHEEEECCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHC
GPAGGLPLLQPMSDIAGRLAPQMGALGLHSSHGGNGKLISGLPGVAPAHVLVIGAGVVGM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCHHHH
AAVRTAIGLGARVTLIDRLRDKLAQAETLFGARVETRFSSPDAIAECVEQADIVIGAAQI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHEEEEECCC
PGRHAPRLISLDLLRRMGAGSVLVDVAIDQGGIAETSRPTTHSDPFFTAEGVVHYCVTNM
CCCCCCCEEEHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCC
PGAVARTATEALTHATLPYVEALAAQGLEALAADPGLHAGLHVHAGKIMHAGLALDLNFD
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]