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The map label for this gene is kdsA

Identifier: 74316638

GI number: 74316638

Start: 652259

End: 653101

Strand: Direct

Name: kdsA

Synonym: Tbd_0620

Alternate gene names: 74316638

Gene position: 652259-653101 (Clockwise)

Preceding gene: 74316637

Following gene: 74316639

Centisome position: 22.42

GC content: 66.07

Gene sequence:

>843_bases
ATGAAGCTGTGTCATTTCGAGGCGGGCCTCGACCAGCCGCTGTTCCTGATCTCGGGCCCTTGCGTGATCGAATCCGAGCA
GCTCGCGATGGACACCGCCGGCCAGCTGAAGGAAATGTGCGCCGGGCTCGGCATTCCGTTCATCTACAAATCGTCGTTCG
ACAAGGCCAATCGCTCGTCGACGAAGTCTTTTCGCGGCCTCGGCCTGGAAGAAGGGCTGCGCATCCTGGCGGACGTGAAG
GCGAAGATCGGCGTGCCCGTGCTGACCGACGTGCACGAGGACACGCCGCTCGACGAGGTGGCGGCCGTGGTCGACGTGCT
GCAGACCCCGGCCTTCCTGTGCCGGCAGACGAATTTCATTCAGAACGTCGCCCGCGCGGGGCGTCCCGTCAACATCAAGA
AGGGCCAGTTCCTCGCGCCGTGGGACATGCAGAACGTCGTCGACAAGGCGCGCGAGGTCGGCAACGACCAGATCATGGTG
TGCGAGCGCGGCGTCTCCTTCGGCTATAACACGCTGGTGTCCGACATGCGCGGCCTCGCGATCATGCGCGGCACGGGGTG
CCCGGTGGTGTTCGACGCGACGCATTCGGTGCAGCAGCCGGGCGGCCAGGGCGCGACGAGCGGCGGGCAGCGCGAGTTCG
TTCCGGTGCTCGCGCGCGCGGCGGTCGCGAGCGGCGTTGCCGGGGTGTTCGCCGAGACGCATCCGGACCCGGCGTGCGCC
CTGTCCGACGGCCCGAACGCGTGGCCGCTCGGCATGATGCGCGAACTGCTGGAAACCTTGAAGGAAATCGACGCGCTGGT
GAAACAGCGCGGGTTTATTGAACACAAGCTGATGAAGGCTTGA

Upstream 100 bases:

>100_bases
GCCAGTTCCATCCGGAATTCACCAGCAACCCGCGCAACGGACACCCGCTGTTCATCTCGTTCGTGAGAGCCGCGCTGGCG
CATCAGCAAAAGGACTCCGC

Downstream 100 bases:

>100_bases
GCGCCGCGGCAGGCACGGGCTCGCGGCCCGTGCTGTTGCAGCGCGGACTGATAAAGACTGAGGAAAAAGATTGAGCGCCA
TTATCGATGTCATCGCCCGG

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKLCHFEAGLDQPLFLISGPCVIESEQLAMDTAGQLKEMCAGLGIPFIYKSSFDKANRSSTKSFRGLGLEEGLRILADVK
AKIGVPVLTDVHEDTPLDEVAAVVDVLQTPAFLCRQTNFIQNVARAGRPVNIKKGQFLAPWDMQNVVDKAREVGNDQIMV
CERGVSFGYNTLVSDMRGLAIMRGTGCPVVFDATHSVQQPGGQGATSGGQREFVPVLARAAVASGVAGVFAETHPDPACA
LSDGPNAWPLGMMRELLETLKEIDALVKQRGFIEHKLMKA

Sequences:

>Translated_280_residues
MKLCHFEAGLDQPLFLISGPCVIESEQLAMDTAGQLKEMCAGLGIPFIYKSSFDKANRSSTKSFRGLGLEEGLRILADVK
AKIGVPVLTDVHEDTPLDEVAAVVDVLQTPAFLCRQTNFIQNVARAGRPVNIKKGQFLAPWDMQNVVDKAREVGNDQIMV
CERGVSFGYNTLVSDMRGLAIMRGTGCPVVFDATHSVQQPGGQGATSGGQREFVPVLARAAVASGVAGVFAETHPDPACA
LSDGPNAWPLGMMRELLETLKEIDALVKQRGFIEHKLMKA
>Mature_280_residues
MKLCHFEAGLDQPLFLISGPCVIESEQLAMDTAGQLKEMCAGLGIPFIYKSSFDKANRSSTKSFRGLGLEEGLRILADVK
AKIGVPVLTDVHEDTPLDEVAAVVDVLQTPAFLCRQTNFIQNVARAGRPVNIKKGQFLAPWDMQNVVDKAREVGNDQIMV
CERGVSFGYNTLVSDMRGLAIMRGTGCPVVFDATHSVQQPGGQGATSGGQREFVPVLARAAVASGVAGVFAETHPDPACA
LSDGPNAWPLGMMRELLETLKEIDALVKQRGFIEHKLMKA

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=266, Percent_Identity=48.4962406015038, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_THIDA (Q3SL44)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314378.1
- HSSP:   O66496
- ProteinModelPortal:   Q3SL44
- SMR:   Q3SL44
- STRING:   Q3SL44
- GeneID:   3673731
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0620
- NMPDR:   fig|292415.3.peg.507
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   IWLTERG
- PhylomeDB:   Q3SL44
- ProtClustDB:   PRK05198
- BioCyc:   TDEN292415:TBD_0620-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30155; Mature: 30155

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCHFEAGLDQPLFLISGPCVIESEQLAMDTAGQLKEMCAGLGIPFIYKSSFDKANRSS
CCEECCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHH
TKSFRGLGLEEGLRILADVKAKIGVPVLTDVHEDTPLDEVAAVVDVLQTPAFLCRQTNFI
HHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
QNVARAGRPVNIKKGQFLAPWDMQNVVDKAREVGNDQIMVCERGVSFGYNTLVSDMRGLA
HHHHHCCCCCEECCCCEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCEE
IMRGTGCPVVFDATHSVQQPGGQGATSGGQREFVPVLARAAVASGVAGVFAETHPDPACA
EEECCCCCEEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LSDGPNAWPLGMMRELLETLKEIDALVKQRGFIEHKLMKA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKLCHFEAGLDQPLFLISGPCVIESEQLAMDTAGQLKEMCAGLGIPFIYKSSFDKANRSS
CCEECCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHH
TKSFRGLGLEEGLRILADVKAKIGVPVLTDVHEDTPLDEVAAVVDVLQTPAFLCRQTNFI
HHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
QNVARAGRPVNIKKGQFLAPWDMQNVVDKAREVGNDQIMVCERGVSFGYNTLVSDMRGLA
HHHHHCCCCCEECCCCEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCEE
IMRGTGCPVVFDATHSVQQPGGQGATSGGQREFVPVLARAAVASGVAGVFAETHPDPACA
EEECCCCCEEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LSDGPNAWPLGMMRELLETLKEIDALVKQRGFIEHKLMKA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA