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The map label for this gene is purA

Identifier: 74316625

GI number: 74316625

Start: 634770

End: 636068

Strand: Direct

Name: purA

Synonym: Tbd_0607

Alternate gene names: 74316625

Gene position: 634770-636068 (Clockwise)

Preceding gene: 74316624

Following gene: 74316626

Centisome position: 21.81

GC content: 66.2

Gene sequence:

>1299_bases
ATGGCAGCAAAAAACGTCGTCGTCATCGGCACCCAATGGGGCGATGAAGGCAAGGGCAAGATCGTCGATTGGCTGACCGA
CCGCGCGCAGGGCGTCGTGCGCTTTCAGGGCGGCCACAATGCAGGCCACACGCTGGTGGTGGGCGGCAAGAAGACGGTGC
TGCACCTGATCCCGTCGGGCATCCTGCGCGACAACGTCACCTGCTACATCGGCAACGGCGTTGTGCTTGCCCCGGATGCG
CTGCTGAAGGAAATGGACATGCTCGAGGCCGCGGGCGTCGACGTCGCGAGCCGCCTGAAGATCAGCGAGGCCTGCCCGCT
GATCATGCCGCACCACGTTGCGCTCGACCAGGCGCGCGAGATCGCCAAGGGCGCCGGCAAGATCGGCACGACCGGGCGCG
GCATCGGGCCGGCCTACGAGGACAAGGTCGCACGCCGCGCGCTGCGCCTGCAGGACCTGTTCCATCGGGAGCGCTTCGCC
GCCAAGCTCGGCGAGGTGCTCGACCACCACAACTTCATGCTGAAGAACTACTACAAGGTCGAAGTGGTGAGCTTCAACCA
GACGCTCGACGGCATGATGGCGATGGCCGAGCGGCTGAAGCCGATGGTCGCCGACGTGCCGCGCAGCCTCTACGAGGCGA
ACAAGGCGGGCGGCAATCTCCTGTTCGAGGGCGCGCAGGGCACGCTGTTGGATATCGATCACGGCACGTATCCCTTCGTG
ACCTCGTCGAACTGCACCGCGGGCGGTGCCGCGACGGGGTCCGGCGTCGGCCCGTCGGCGATGCACTACGTGCTCGGCAT
CACCAAGGCCTACACGACGCGCGTCGGTTCCGGCCCCTTCCCGACCGAACTGTTCGACGACGTCGGCCAGTATCTGGGCG
AGAAGGGCCACGAATTCGGCGCGACCACCGGCCGCCAGCGCCGCTGCGGATGGTTCGACGCCGCCGCGCTCAAGCGCTCG
ATCCAGATCAACGGCGTCTCGGGTTTGTGCGTGACCAAGCTCGACGTGCTCGACGGGCTGGAGACCGTGCGCGTCTGCGT
CGGCTATAAAATCGACGGCGAGGCCTGCGACATCTTGCCGGTCGGCGCCGAGTCGATCACCAACGTCGAGCCGATCTACG
AAGATCTGCCCGGCTGGAGCGACACGACGGTGGGCGTGCAGATGTTCGACAAGCTGCCGCAGAAGGCGCAGACCTATCTC
AAGCGGATGGAAGAACTGTGCGAAGTGCCGGTCGACGTCGTCTCGACGGGGCCCGACCGTGTCGAGACGATCGTGCGTCG
TCACCCCTACGAGGCCTGA

Upstream 100 bases:

>100_bases
GCCGCGTGTTCGGACGGGCGCGTCCGGCGACCGGCTTCAGTATGGATTTACGCGAGCTGGTCGTTGCCGGCTCGGCTTCA
GAATAAGGGAGATTCCAACC

Downstream 100 bases:

>100_bases
TGGCTAGAATAGAGTCCGAAAAAGTAAAAAGCCGACACGAGGTCGGCTTTTTACTTGCAGCGTCTGGTGCCCAGAAGAGG
ACTCGAACCTCCACAGTGTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAAKNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHTLVVGGKKTVLHLIPSGILRDNVTCYIGNGVVLAPDA
LLKEMDMLEAAGVDVASRLKISEACPLIMPHHVALDQAREIAKGAGKIGTTGRGIGPAYEDKVARRALRLQDLFHRERFA
AKLGEVLDHHNFMLKNYYKVEVVSFNQTLDGMMAMAERLKPMVADVPRSLYEANKAGGNLLFEGAQGTLLDIDHGTYPFV
TSSNCTAGGAATGSGVGPSAMHYVLGITKAYTTRVGSGPFPTELFDDVGQYLGEKGHEFGATTGRQRRCGWFDAAALKRS
IQINGVSGLCVTKLDVLDGLETVRVCVGYKIDGEACDILPVGAESITNVEPIYEDLPGWSDTTVGVQMFDKLPQKAQTYL
KRMEELCEVPVDVVSTGPDRVETIVRRHPYEA

Sequences:

>Translated_432_residues
MAAKNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHTLVVGGKKTVLHLIPSGILRDNVTCYIGNGVVLAPDA
LLKEMDMLEAAGVDVASRLKISEACPLIMPHHVALDQAREIAKGAGKIGTTGRGIGPAYEDKVARRALRLQDLFHRERFA
AKLGEVLDHHNFMLKNYYKVEVVSFNQTLDGMMAMAERLKPMVADVPRSLYEANKAGGNLLFEGAQGTLLDIDHGTYPFV
TSSNCTAGGAATGSGVGPSAMHYVLGITKAYTTRVGSGPFPTELFDDVGQYLGEKGHEFGATTGRQRRCGWFDAAALKRS
IQINGVSGLCVTKLDVLDGLETVRVCVGYKIDGEACDILPVGAESITNVEPIYEDLPGWSDTTVGVQMFDKLPQKAQTYL
KRMEELCEVPVDVVSTGPDRVETIVRRHPYEA
>Mature_431_residues
AAKNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHTLVVGGKKTVLHLIPSGILRDNVTCYIGNGVVLAPDAL
LKEMDMLEAAGVDVASRLKISEACPLIMPHHVALDQAREIAKGAGKIGTTGRGIGPAYEDKVARRALRLQDLFHRERFAA
KLGEVLDHHNFMLKNYYKVEVVSFNQTLDGMMAMAERLKPMVADVPRSLYEANKAGGNLLFEGAQGTLLDIDHGTYPFVT
SSNCTAGGAATGSGVGPSAMHYVLGITKAYTTRVGSGPFPTELFDDVGQYLGEKGHEFGATTGRQRRCGWFDAAALKRSI
QINGVSGLCVTKLDVLDGLETVRVCVGYKIDGEACDILPVGAESITNVEPIYEDLPGWSDTTVGVQMFDKLPQKAQTYLK
RMEELCEVPVDVVSTGPDRVETIVRRHPYEA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=43.6915887850467, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=43.7647058823529, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=42.7848101265823, Blast_Score=299, Evalue=3e-81,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=63.953488372093, Blast_Score=575, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=43.3874709976798, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=43.3874709976798, Blast_Score=341, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=425, Percent_Identity=42.8235294117647, Blast_Score=331, Evalue=2e-91,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=41.7249417249417, Blast_Score=324, Evalue=9e-89,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_THIDA (Q3SL57)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314365.1
- ProteinModelPortal:   Q3SL57
- SMR:   Q3SL57
- STRING:   Q3SL57
- GeneID:   3673717
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0607
- NMPDR:   fig|292415.3.peg.451
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q3SL57
- ProtClustDB:   PRK01117
- BioCyc:   TDEN292415:TBD_0607-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46657; Mature: 46526

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 15-15 ACT_SITE 43-43 BINDING 131-131 BINDING 145-145 BINDING 226-226 BINDING 241-241 BINDING 305-305 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHTLVVGGKKTVLHLIPSG
CCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEECCCHHEEHHCCCH
ILRDNVTCYIGNGVVLAPDALLKEMDMLEAAGVDVASRLKISEACPLIMPHHVALDQARE
HHHCCCEEEECCCEEECCHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCCCHHHHHHHHHH
IAKGAGKIGTTGRGIGPAYEDKVARRALRLQDLFHRERFAAKLGEVLDHHNFMLKNYYKV
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEE
EVVSFNQTLDGMMAMAERLKPMVADVPRSLYEANKAGGNLLFEGAQGTLLDIDHGTYPFV
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEE
TSSNCTAGGAATGSGVGPSAMHYVLGITKAYTTRVGSGPFPTELFDDVGQYLGEKGHEFG
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
ATTGRQRRCGWFDAAALKRSIQINGVSGLCVTKLDVLDGLETVRVCVGYKIDGEACDILP
CCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEE
VGAESITNVEPIYEDLPGWSDTTVGVQMFDKLPQKAQTYLKRMEELCEVPVDVVSTGPDR
CCHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCHHH
VETIVRRHPYEA
HHHHHHHCCCCC
>Mature Secondary Structure 
AAKNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHTLVVGGKKTVLHLIPSG
CCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEECCCHHEEHHCCCH
ILRDNVTCYIGNGVVLAPDALLKEMDMLEAAGVDVASRLKISEACPLIMPHHVALDQARE
HHHCCCEEEECCCEEECCHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCCCHHHHHHHHHH
IAKGAGKIGTTGRGIGPAYEDKVARRALRLQDLFHRERFAAKLGEVLDHHNFMLKNYYKV
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEE
EVVSFNQTLDGMMAMAERLKPMVADVPRSLYEANKAGGNLLFEGAQGTLLDIDHGTYPFV
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEE
TSSNCTAGGAATGSGVGPSAMHYVLGITKAYTTRVGSGPFPTELFDDVGQYLGEKGHEFG
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
ATTGRQRRCGWFDAAALKRSIQINGVSGLCVTKLDVLDGLETVRVCVGYKIDGEACDILP
CCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEE
VGAESITNVEPIYEDLPGWSDTTVGVQMFDKLPQKAQTYLKRMEELCEVPVDVVSTGPDR
CCHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCHHH
VETIVRRHPYEA
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA