Click here to switch to the map view.

The map label for this gene is hisZ

Identifier: 74316624

GI number: 74316624

Start: 633790

End: 634755

Strand: Direct

Name: hisZ

Synonym: Tbd_0606

Alternate gene names: 74316624

Gene position: 633790-634755 (Clockwise)

Preceding gene: 74316623

Following gene: 74316625

Centisome position: 21.78

GC content: 68.53

Gene sequence:

>966_bases
ATGACTGCCTGGTTGCTGCCCGAAAACGTCGAGGATGTGCTGCCGCCACAGGCCTGGCGCCTCGAAGCGATGCGGCGCGC
GCTGCTCGACCTGTTCCGCGAGCGCGGCTATCAGCTCGTGATCCCGCCGCTCATCGAGTACGTCGAGTCGCTGCTGACCG
GCGTCGGCGCCGATCTCGACCTCAAGACGTTCAAGCTGGTCGATCAGCTCTCCGGCCGTCTCATGGCGGTGCGTGCCGAC
ATCACGCCTCAGGTCGCGCGCATTGATGCCCACCTGCTAGGTGCCAACGCGATCAACCGCCTGTGTTACACCGGCAGCGT
CCTGCACACCCAGTCCGACGGCTTTCACCGTTCGCGCGAACCGATCCAGATCGGCGCCGAGGTCTACGGCGACGCCGGCA
TCGAAGCCGACCTCGAAATCCTCAGCCTGATGCTGCAGGGGCTGGCGGCGTGCGGGGTCGAGGGCGTACAACTCGACGTC
GGGCACGTCGGCGTCTACCGCGCGCTGGCGCAGGAAGCCGGGTTCGACAACGCGACCGAGCACGAGCTGTTCTGCGCGCT
GCAGGCGAAGGACGCGAGTGCGGTCGACGTGCTCACGGCAGGTCTGCCGATGGCGCTGCGCGACGCGTTCGCGGCGCTGC
CGCAGCTGTATGGCGGCCGTGAGGTGCTGGCCGAGGCGCGTGCCCGCTTGCCGCACCTCCCGGCCGTCGCCGCGGCGCTC
GATACGCTGGTGTCGCTCGACCAGGCGCTCGGCGGTGTCGAACTCGCCTACGACCTTGCCGAGCTGCGCGGCTACGGCTA
CCACAGCGGCGTGGTGTTCGCGGCCTATACCCGCGGACGCAGCCATGCCATCGCACAGGGTGGGCGCTACGACGAGGTCG
GCCGCGTGTTCGGACGGGCGCGTCCGGCGACCGGCTTCAGTATGGATTTACGCGAGCTGGTCGTTGCCGGCTCGGCTTCA
GAATAA

Upstream 100 bases:

>100_bases
GGTTTCAGCAGGATGCTGGCACTGCGCGACGGTCAGCTGCGTTTCATTGGAGCCACCTCGATGCTGGGTGGCGTTTTTTT
GTTGATGTTGTCGAATTGAA

Downstream 100 bases:

>100_bases
GGGAGATTCCAACCATGGCAGCAAAAAACGTCGTCGTCATCGGCACCCAATGGGGCGATGAAGGCAAGGGCAAGATCGTC
GATTGGCTGACCGACCGCGC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTAWLLPENVEDVLPPQAWRLEAMRRALLDLFRERGYQLVIPPLIEYVESLLTGVGADLDLKTFKLVDQLSGRLMAVRAD
ITPQVARIDAHLLGANAINRLCYTGSVLHTQSDGFHRSREPIQIGAEVYGDAGIEADLEILSLMLQGLAACGVEGVQLDV
GHVGVYRALAQEAGFDNATEHELFCALQAKDASAVDVLTAGLPMALRDAFAALPQLYGGREVLAEARARLPHLPAVAAAL
DTLVSLDQALGGVELAYDLAELRGYGYHSGVVFAAYTRGRSHAIAQGGRYDEVGRVFGRARPATGFSMDLRELVVAGSAS
E

Sequences:

>Translated_321_residues
MTAWLLPENVEDVLPPQAWRLEAMRRALLDLFRERGYQLVIPPLIEYVESLLTGVGADLDLKTFKLVDQLSGRLMAVRAD
ITPQVARIDAHLLGANAINRLCYTGSVLHTQSDGFHRSREPIQIGAEVYGDAGIEADLEILSLMLQGLAACGVEGVQLDV
GHVGVYRALAQEAGFDNATEHELFCALQAKDASAVDVLTAGLPMALRDAFAALPQLYGGREVLAEARARLPHLPAVAAAL
DTLVSLDQALGGVELAYDLAELRGYGYHSGVVFAAYTRGRSHAIAQGGRYDEVGRVFGRARPATGFSMDLRELVVAGSAS
E
>Mature_320_residues
TAWLLPENVEDVLPPQAWRLEAMRRALLDLFRERGYQLVIPPLIEYVESLLTGVGADLDLKTFKLVDQLSGRLMAVRADI
TPQVARIDAHLLGANAINRLCYTGSVLHTQSDGFHRSREPIQIGAEVYGDAGIEADLEILSLMLQGLAACGVEGVQLDVG
HVGVYRALAQEAGFDNATEHELFCALQAKDASAVDVLTAGLPMALRDAFAALPQLYGGREVLAEARARLPHLPAVAAALD
TLVSLDQALGGVELAYDLAELRGYGYHSGVVFAAYTRGRSHAIAQGGRYDEVGRVFGRARPATGFSMDLRELVVAGSASE

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_THIDA (Q3SL58)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314364.1
- ProteinModelPortal:   Q3SL58
- SMR:   Q3SL58
- STRING:   Q3SL58
- GeneID:   3673716
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0606
- NMPDR:   fig|292415.3.peg.450
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- BioCyc:   TDEN292415:TBD_0606-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 34505; Mature: 34374

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAWLLPENVEDVLPPQAWRLEAMRRALLDLFRERGYQLVIPPLIEYVESLLTGVGADLD
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCC
LKTFKLVDQLSGRLMAVRADITPQVARIDAHLLGANAINRLCYTGSVLHTQSDGFHRSRE
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
PIQIGAEVYGDAGIEADLEILSLMLQGLAACGVEGVQLDVGHVGVYRALAQEAGFDNATE
CCEECHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCC
HELFCALQAKDASAVDVLTAGLPMALRDAFAALPQLYGGREVLAEARARLPHLPAVAAAL
CCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
DTLVSLDQALGGVELAYDLAELRGYGYHSGVVFAAYTRGRSHAIAQGGRYDEVGRVFGRA
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCHHHHHHHHHCC
RPATGFSMDLRELVVAGSASE
CCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAWLLPENVEDVLPPQAWRLEAMRRALLDLFRERGYQLVIPPLIEYVESLLTGVGADLD
CCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCC
LKTFKLVDQLSGRLMAVRADITPQVARIDAHLLGANAINRLCYTGSVLHTQSDGFHRSRE
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
PIQIGAEVYGDAGIEADLEILSLMLQGLAACGVEGVQLDVGHVGVYRALAQEAGFDNATE
CCEECHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCC
HELFCALQAKDASAVDVLTAGLPMALRDAFAALPQLYGGREVLAEARARLPHLPAVAAAL
CCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
DTLVSLDQALGGVELAYDLAELRGYGYHSGVVFAAYTRGRSHAIAQGGRYDEVGRVFGRA
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCHHHHHHHHHCC
RPATGFSMDLRELVVAGSASE
CCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA