Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 74316613

GI number: 74316613

Start: 623727

End: 624986

Strand: Direct

Name: hisS

Synonym: Tbd_0595

Alternate gene names: 74316613

Gene position: 623727-624986 (Clockwise)

Preceding gene: 74316612

Following gene: 74316614

Centisome position: 21.44

GC content: 66.75

Gene sequence:

>1260_bases
ATGAGTGAGAACATCCAATCCGTCCGCGGCATGAACGACTGCCTGCCCGACGCGGCCGACGCCTGGCAGGGTTTCGAGGC
GATCGTGCGCGACTGGCTGCGGCGCTACGGCTACCGCGAGATGCGCACGCCGATCCTCGAACACACGGGCCTGTTCAAGC
GCGCGATCGGCGAGGTGACCGACATCGTCGAGAAGGAGATGTATACCTTCGTCGACGAACTCAACGGCGAATCCCTGACC
CTGCGTCCCGAGGGCACGGCGTCGAGCGTGCGCGCCGTGATCCAGCACAACCTGCTTTACGACGGCGGCAAGCGTCTCTG
GTATAGCGGTCCGATGTTCCGTCACGAGCGCCCGCAGAAGGGTCGCTACCGGCAGTTCCATCAGGTCGGCGTCGAGGCGC
TCGGCTTCGCCGGTCCCGACGTCGACGCCGAGCTGATCGTGATGTGCGCCGACCTCTGGCGCGAGCTCGGCATCGCGCCG
ACGCTGCAACTCAATACGCTCGGTGACCACGGCGCGCGCCAGCGCCACCGCAGCAAGCTGATCGCGTATTACGAGGCGCA
TCGCGACGTGCTCGACGCCGACGCCCAGCGGCGTCTGCACAGCAATCCGCTGCGCATTCTCGACAGCAAGAATCCGGCAA
TGCAGGCGCTGAACGCCGAAGCGCCACGCTTGCTGGACGAACTCGAGGACGAGGCCCTGAATCATTTCGACGCGCTGCAG
GGCCTGTTGCGCGCGAACGGCATCGCCTTCGAGATCAACCCGCGCCTGGTCCGCGGACTGGACTATTACAATCGCACCGT
TTTCGAGTGGGTCACCGACCAGCTTGGCGCGCAGGGCACCGTCTGCGCGGGCGGTCGTTACGACGGCCTGGTCGAGCAAC
TCGGCGGCAAACCCGCGCCCGCGGCGGGCTTCGCGATGGGCATCGAACGCCTGCTGGCGCTGGTTGAAACGAGCGGCAAG
CCGATCGTCCCGGCCGTACCCGACGCCTACATCGTCCACGCCGGCGACACGGCCGACGCCTTCGCCTGGCAGGCAGCGGC
TACACTGCGGGGCGCCGGCCTCGCGGTGGTCTTGCATTGCGGCGGCGGCAGCTTCAAGTCGCAGATGAAAAAAGCCGACG
CGAGCCGTGCGCGCTACGCGGTCATCATCGGCGACGAGGAGGCGGCGGCGCAGCAGGTGAGCGTGAAACCGCTGCGCGGC
ACGGCGGAGCAGACCCGCGTCGAACTCGCGCTCGCAATCGAATATCTGAAAAAGGCATAA

Upstream 100 bases:

>100_bases
AAGGGCGACCGCATCGCCGCGGAGTTCCAGGCGATCGTCGAAGACTACGTGCGCACGCGGTACGGCAGCGCCTGAGCCCG
CCCTTGACCATCCAGAATTT

Downstream 100 bases:

>100_bases
GCACCCATGGCAACCTACGACCTCGATGAACAGGAACGGCTCGACGAACTGAAAGCCTGGTGGAAACGCTGGGGCAGCGT
CTCAATGATCGGGCTGGCGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSENIQSVRGMNDCLPDAADAWQGFEAIVRDWLRRYGYREMRTPILEHTGLFKRAIGEVTDIVEKEMYTFVDELNGESLT
LRPEGTASSVRAVIQHNLLYDGGKRLWYSGPMFRHERPQKGRYRQFHQVGVEALGFAGPDVDAELIVMCADLWRELGIAP
TLQLNTLGDHGARQRHRSKLIAYYEAHRDVLDADAQRRLHSNPLRILDSKNPAMQALNAEAPRLLDELEDEALNHFDALQ
GLLRANGIAFEINPRLVRGLDYYNRTVFEWVTDQLGAQGTVCAGGRYDGLVEQLGGKPAPAAGFAMGIERLLALVETSGK
PIVPAVPDAYIVHAGDTADAFAWQAAATLRGAGLAVVLHCGGGSFKSQMKKADASRARYAVIIGDEEAAAQQVSVKPLRG
TAEQTRVELALAIEYLKKA

Sequences:

>Translated_419_residues
MSENIQSVRGMNDCLPDAADAWQGFEAIVRDWLRRYGYREMRTPILEHTGLFKRAIGEVTDIVEKEMYTFVDELNGESLT
LRPEGTASSVRAVIQHNLLYDGGKRLWYSGPMFRHERPQKGRYRQFHQVGVEALGFAGPDVDAELIVMCADLWRELGIAP
TLQLNTLGDHGARQRHRSKLIAYYEAHRDVLDADAQRRLHSNPLRILDSKNPAMQALNAEAPRLLDELEDEALNHFDALQ
GLLRANGIAFEINPRLVRGLDYYNRTVFEWVTDQLGAQGTVCAGGRYDGLVEQLGGKPAPAAGFAMGIERLLALVETSGK
PIVPAVPDAYIVHAGDTADAFAWQAAATLRGAGLAVVLHCGGGSFKSQMKKADASRARYAVIIGDEEAAAQQVSVKPLRG
TAEQTRVELALAIEYLKKA
>Mature_418_residues
SENIQSVRGMNDCLPDAADAWQGFEAIVRDWLRRYGYREMRTPILEHTGLFKRAIGEVTDIVEKEMYTFVDELNGESLTL
RPEGTASSVRAVIQHNLLYDGGKRLWYSGPMFRHERPQKGRYRQFHQVGVEALGFAGPDVDAELIVMCADLWRELGIAPT
LQLNTLGDHGARQRHRSKLIAYYEAHRDVLDADAQRRLHSNPLRILDSKNPAMQALNAEAPRLLDELEDEALNHFDALQG
LLRANGIAFEINPRLVRGLDYYNRTVFEWVTDQLGAQGTVCAGGRYDGLVEQLGGKPAPAAGFAMGIERLLALVETSGKP
IVPAVPDAYIVHAGDTADAFAWQAAATLRGAGLAVVLHCGGGSFKSQMKKADASRARYAVIIGDEEAAAQQVSVKPLRGT
AEQTRVELALAIEYLKKA

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=413, Percent_Identity=56.9007263922518, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_THIDA (Q3SL69)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314353.1
- ProteinModelPortal:   Q3SL69
- SMR:   Q3SL69
- STRING:   Q3SL69
- GeneID:   3673705
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0595
- NMPDR:   fig|292415.3.peg.397
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q3SL69
- ProtClustDB:   PRK00037
- BioCyc:   TDEN292415:TBD_0595-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46233; Mature: 46102

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENIQSVRGMNDCLPDAADAWQGFEAIVRDWLRRYGYREMRTPILEHTGLFKRAIGEVT
CCCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHH
DIVEKEMYTFVDELNGESLTLRPEGTASSVRAVIQHNLLYDGGKRLWYSGPMFRHERPQK
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
GRYRQFHQVGVEALGFAGPDVDAELIVMCADLWRELGIAPTLQLNTLGDHGARQRHRSKL
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
IAYYEAHRDVLDADAQRRLHSNPLRILDSKNPAMQALNAEAPRLLDELEDEALNHFDALQ
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GLLRANGIAFEINPRLVRGLDYYNRTVFEWVTDQLGAQGTVCAGGRYDGLVEQLGGKPAP
HHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCC
AAGFAMGIERLLALVETSGKPIVPAVPDAYIVHAGDTADAFAWQAAATLRGAGLAVVLHC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEC
GGGSFKSQMKKADASRARYAVIIGDEEAAAQQVSVKPLRGTAEQTRVELALAIEYLKKA
CCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SENIQSVRGMNDCLPDAADAWQGFEAIVRDWLRRYGYREMRTPILEHTGLFKRAIGEVT
CCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHH
DIVEKEMYTFVDELNGESLTLRPEGTASSVRAVIQHNLLYDGGKRLWYSGPMFRHERPQK
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
GRYRQFHQVGVEALGFAGPDVDAELIVMCADLWRELGIAPTLQLNTLGDHGARQRHRSKL
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
IAYYEAHRDVLDADAQRRLHSNPLRILDSKNPAMQALNAEAPRLLDELEDEALNHFDALQ
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GLLRANGIAFEINPRLVRGLDYYNRTVFEWVTDQLGAQGTVCAGGRYDGLVEQLGGKPAP
HHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCC
AAGFAMGIERLLALVETSGKPIVPAVPDAYIVHAGDTADAFAWQAAATLRGAGLAVVLHC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEC
GGGSFKSQMKKADASRARYAVIIGDEEAAAQQVSVKPLRGTAEQTRVELALAIEYLKKA
CCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA