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The map label for this gene is ispG [H]

Identifier: 74316612

GI number: 74316612

Start: 622478

End: 623701

Strand: Direct

Name: ispG [H]

Synonym: Tbd_0594

Alternate gene names: 74316612

Gene position: 622478-623701 (Clockwise)

Preceding gene: 74316611

Following gene: 74316613

Centisome position: 21.39

GC content: 68.3

Gene sequence:

>1224_bases
ATGACGACTCAAATTGTCCGCCGCACCACCCGTCAGGTGCGGATCGGCCCGATATGCGTAGGCGGCGACGCGCCGGTGGC
GGTGCAGTCGATGACCAACACCGACACGGTCGACGTCAATGCGACCGTAAGCCAGGTGCAGGCGCTCGCTGCCGCCGGCT
CGGAACTCGTGCGGCTCACCGTGAACACGCTGGAAGCGGCGGCGGCCGTGCCGCGCATCCGCGAGCGCCTCGATGTGCTG
GGCTGTCGCGTGCCGCTGATCGGCGACTTCCACTTCAACGGCCACAAGCTCCTGACCCAGGTCCCCGAGTGCGCCCAGGC
GCTGGCCAAACTCCGGATCAATCCCGGCAACATCGGCCGCGGCGAGAAACGCGACGAGCAGTTCGCGACGCTGATCGACG
TCGCCTGCCGTCACGACAAGCCGATCCGCATCGGCGTCAACTGGGGCAGTCTCGACCAGGCGCTGGCCGCGCGCATGATG
GACGACAACGCCAGGCGCGCGCAGCCTGAAGATGCCGAGGTCGTCATGCGCCGGGCGATGGTCGCGTCGGCCCTCGAGTC
GGCGCGCAAGGCCGAGGAACTCGGATTGGGCGGCGACAGGATCATCCTCTCCTGCAAGATGAGCCGGGTACAGGACTTGA
TCGCGGTTTACCGGGATCTCGCGGCGCAGTGCGACTATCCGCTCCACCTCGGCCTCACCGAGGCCGGCATGGGCAGCAAG
GGCATCGTCGCCTCGACCGCGGCGCTCGCGGTACTGCTGCAGGAGGGGATCGGCGACACGATACGCATTTCGCTGACCCC
CGAGCCGGGCGGCGACCGCGCCCAGGAAGTCCGCGTCGGCCAGGAAATCCTGCAGGCGCTCGGCCTGCGCGCCTTCACGC
CGCAAGTCACGGCATGCCCGGGGTGCGGCCGCACGACCTCGACCGTTTTCCAGGAGCTCGCGCAGGATATCGAGACCTAT
CTGCGCACGCAGATGCCGATCTGGCGCAGCCGCTACCCCGGGGTCGAAACCATGCAGGTGGCGGTCATGGGCTGCGTCGT
CAACGGCCCTGGCGAATCCAAGCACGCGAACATCGGAATCTCGCTGCCCGGCACGGGCGAAGTGCCGGTCGCGCCGGTCT
ACGTCGACGGCGAAAAGACCGTGACGCTCAAGGGCGACCGCATCGCCGCGGAGTTCCAGGCGATCGTCGAAGACTACGTG
CGCACGCGGTACGGCAGCGCCTGA

Upstream 100 bases:

>100_bases
CGCGGCGGAGACGCGCCTGACCTACAACGGTCGCCCCGTCGATTTGAAACCCCATACCGGCATGACGGTCGCCCGCTTCA
CGCTCGAATAGATTCCCCAG

Downstream 100 bases:

>100_bases
GCCCGCCCTTGACCATCCAGAATTTATGAGTGAGAACATCCAATCCGTCCGCGGCATGAACGACTGCCTGCCCGACGCGG
CCGACGCCTGGCAGGGTTTC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTTQIVRRTTRQVRIGPICVGGDAPVAVQSMTNTDTVDVNATVSQVQALAAAGSELVRLTVNTLEAAAAVPRIRERLDVL
GCRVPLIGDFHFNGHKLLTQVPECAQALAKLRINPGNIGRGEKRDEQFATLIDVACRHDKPIRIGVNWGSLDQALAARMM
DDNARRAQPEDAEVVMRRAMVASALESARKAEELGLGGDRIILSCKMSRVQDLIAVYRDLAAQCDYPLHLGLTEAGMGSK
GIVASTAALAVLLQEGIGDTIRISLTPEPGGDRAQEVRVGQEILQALGLRAFTPQVTACPGCGRTTSTVFQELAQDIETY
LRTQMPIWRSRYPGVETMQVAVMGCVVNGPGESKHANIGISLPGTGEVPVAPVYVDGEKTVTLKGDRIAAEFQAIVEDYV
RTRYGSA

Sequences:

>Translated_407_residues
MTTQIVRRTTRQVRIGPICVGGDAPVAVQSMTNTDTVDVNATVSQVQALAAAGSELVRLTVNTLEAAAAVPRIRERLDVL
GCRVPLIGDFHFNGHKLLTQVPECAQALAKLRINPGNIGRGEKRDEQFATLIDVACRHDKPIRIGVNWGSLDQALAARMM
DDNARRAQPEDAEVVMRRAMVASALESARKAEELGLGGDRIILSCKMSRVQDLIAVYRDLAAQCDYPLHLGLTEAGMGSK
GIVASTAALAVLLQEGIGDTIRISLTPEPGGDRAQEVRVGQEILQALGLRAFTPQVTACPGCGRTTSTVFQELAQDIETY
LRTQMPIWRSRYPGVETMQVAVMGCVVNGPGESKHANIGISLPGTGEVPVAPVYVDGEKTVTLKGDRIAAEFQAIVEDYV
RTRYGSA
>Mature_406_residues
TTQIVRRTTRQVRIGPICVGGDAPVAVQSMTNTDTVDVNATVSQVQALAAAGSELVRLTVNTLEAAAAVPRIRERLDVLG
CRVPLIGDFHFNGHKLLTQVPECAQALAKLRINPGNIGRGEKRDEQFATLIDVACRHDKPIRIGVNWGSLDQALAARMMD
DNARRAQPEDAEVVMRRAMVASALESARKAEELGLGGDRIILSCKMSRVQDLIAVYRDLAAQCDYPLHLGLTEAGMGSKG
IVASTAALAVLLQEGIGDTIRISLTPEPGGDRAQEVRVGQEILQALGLRAFTPQVTACPGCGRTTSTVFQELAQDIETYL
RTQMPIWRSRYPGVETMQVAVMGCVVNGPGESKHANIGISLPGTGEVPVAPVYVDGEKTVTLKGDRIAAEFQAIVEDYVR
TRYGSA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=393, Percent_Identity=35.6234096692112, Blast_Score=207, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 43706; Mature: 43575

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQIVRRTTRQVRIGPICVGGDAPVAVQSMTNTDTVDVNATVSQVQALAAAGSELVRLT
CCHHHHHHHHHHEEECCEEECCCCCEEEEECCCCCEEEECHHHHHHHHHHHCCCCEEEEH
VNTLEAAAAVPRIRERLDVLGCRVPLIGDFHFNGHKLLTQVPECAQALAKLRINPGNIGR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEECCCCCCCC
GEKRDEQFATLIDVACRHDKPIRIGVNWGSLDQALAARMMDDNARRAQPEDAEVVMRRAM
CCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VASALESARKAEELGLGGDRIILSCKMSRVQDLIAVYRDLAAQCDYPLHLGLTEAGMGSK
HHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GIVASTAALAVLLQEGIGDTIRISLTPEPGGDRAQEVRVGQEILQALGLRAFTPQVTACP
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCCC
GCGRTTSTVFQELAQDIETYLRTQMPIWRSRYPGVETMQVAVMGCVVNGPGESKHANIGI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHEEEEEEEECCCCCCCCCEEEE
SLPGTGEVPVAPVYVDGEKTVTLKGDRIAAEFQAIVEDYVRTRYGSA
ECCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTQIVRRTTRQVRIGPICVGGDAPVAVQSMTNTDTVDVNATVSQVQALAAAGSELVRLT
CHHHHHHHHHHEEECCEEECCCCCEEEEECCCCCEEEECHHHHHHHHHHHCCCCEEEEH
VNTLEAAAAVPRIRERLDVLGCRVPLIGDFHFNGHKLLTQVPECAQALAKLRINPGNIGR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEECCCCCCCC
GEKRDEQFATLIDVACRHDKPIRIGVNWGSLDQALAARMMDDNARRAQPEDAEVVMRRAM
CCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VASALESARKAEELGLGGDRIILSCKMSRVQDLIAVYRDLAAQCDYPLHLGLTEAGMGSK
HHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GIVASTAALAVLLQEGIGDTIRISLTPEPGGDRAQEVRVGQEILQALGLRAFTPQVTACP
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCCC
GCGRTTSTVFQELAQDIETYLRTQMPIWRSRYPGVETMQVAVMGCVVNGPGESKHANIGI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHEEEEEEEECCCCCCCCCEEEE
SLPGTGEVPVAPVYVDGEKTVTLKGDRIAAEFQAIVEDYVRTRYGSA
ECCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA