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The map label for this gene is yfkN [H]

Identifier: 74316581

GI number: 74316581

Start: 591642

End: 593474

Strand: Reverse

Name: yfkN [H]

Synonym: Tbd_0563

Alternate gene names: 74316581

Gene position: 593474-591642 (Counterclockwise)

Preceding gene: 74316582

Following gene: 74316580

Centisome position: 20.4

GC content: 64.21

Gene sequence:

>1833_bases
GTGGAACATGGGCAGGAGCTACTATTTACCGGGCTCGCCGGCAAACTGACAATAGAGCCGATTCCAGCTACCGATTCCGA
GGAGACCCCCATGCACATGAACCGCCGCGAGTTCCTGCAACTGCTCGCCGTCGCGGCCGCGTCCGGCATGGCGCTGGACA
GCAAGTCCGCGCTCGCCGGCAAGGCCCCGGCCAATTTCTACGACGTGCCGCGTCACGGCAACGTCTCCTTCCTGCACTTC
ACCGACTGCCACGCCCAGCTGCTGCCGGTGTGGTTCCGCGAGCCCAACGTCAACCTCGGGGTCGGCGCCGCGCACGGCAA
GGCGCCGCACCTGGTCGGCCAGCATCTGCTCAAACAGTTCGGCATCAAGCCGGGCAGCGCCGAAGCCCATGCCTTCACTT
ACCTCGACTTCACCGAAGCGGCCAAGGTCTACGGCAAGGTCGGGGGCTTCGCCCATCTGAAAACGCTCGTGGACAAGCTG
CGCGCGCAGCGCCCGGGCGCGCTGCTGCTCGACGGCGGCGATACCTGGCAGGGCTCGGCGACTTCGCTGTGGACCAACGC
TCAGGACATGGTCGACGCCTGCATCAAGCTTGGCGTCAATGTCATGACACCCCACTGGGAGTCGACCTTCGGCGCCGAGC
GCGTGCTGGAAATCGTGAACGGCGACTTCAAGAAGGCGAACATCGATTTCGTCGCGCAGAACGTCGTCACCAACGACTTC
GGCGACCAGGTGTTCAAGCCCTACGTCATGAAGGACATGAACGGCGTCAAGGTCGCGGTGATCGGCCAGGCTTTCCCCTA
CACGCCGATCGCGAACCCGCGCCACATGGTGCCGGACTGGAGCTTCGGCATCCGTGACGACAGCATGCAGCAGTTCGTCG
ACCAGGCCCGCGCCGAAGGCGCGAAGGTCGTCGTCGTGCTCTCGCACAACGGCATGGACGTCGACCTCAAGATGGCGAGC
CGCGTGACCGGCATCGACGCGATCTTCGGCGGACACACCCACGACGGCGTGCCGCAGCCGACGAAAGTCAAGAACGCAAA
GGGCGTGACGCTGGTCACCAATGCCGGCTCCAACGGCAAGTTCCTCGGTGTCATGGACTTCGACGTGCGCGGCGGCAAGG
TGCAGAGCTACAAATACCGCCTGCTGCCCGTGTTCTCCAACCTGCTGCCCGCCGACCCGGGCATGGACGCCTTCATCAAG
CAGGTGCGCGCGCCCTACGAAGCCAAGCTGAGCGAAAAGCTCGCCGTCACCGACGACTTCCTCTACCGCCGCGGCAACTT
CAACGGCACCTGGGACCAGTTGCTGGTCGACGCGCTGATGGAGGTCAAGGGCGCCGACGCGGCGTTCTCGCCCGGTTTCC
GCTGGGGGACCACGCTGCTGCCCGGTGACGCGATCACGATGGAACGGCTGATGGACCAGACCGCGATCACCTATCCGCAG
ACCACGCTCACCGAGATGACGGGCGAAACGATCAAGACGATCATGGAAGACGTCGCCGACAATCTGTTCAACGCCGACCC
GTACTACCAGCAGGGCGGCGACATGGTCCGCGTCGGCGGCATCGAGTACACGATCGACCCGAACAAGAAGATCGGCCAGC
GCATCGGCGACATGAAGCTGAACGGGAAACCGGTCAGCGCCGACAAGACCTACATGGTCGCGGGCTGGGCGCCGGTCGGC
GAAGGCGTCCAAGGCGAACCGGTATGGGACGTCGTCGCCACTTACCTGCGCGACAAGAAAGTGATCAAAGGCCTGAAGCT
CAACGAACCGAAGATCGTCGGCGTGGGCAAATCCAATCCAGGCATCGCTGCCTACTCGGGCGGCCTCTCGTAA

Upstream 100 bases:

>100_bases
CAACGGCCTGCCGATGAAGGCATCGGTGTTCCGCAAGTAAACACGCCTCGCGCACCCCGAGAAAAAAGCCCGGCCAACCG
GGCTTTTTCTTTGTTCCATC

Downstream 100 bases:

>100_bases
GGCGCGTGACGACACGCTGAACAAAAAGGGGCGAAAGCCCCTTTTTTTCATTCTCTCCTTACTGCCCCTATGCCGGCGGG
GCGGCCGCGCTGGTCACGAG

Product: sulfate thiol esterase SoxB

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MEHGQELLFTGLAGKLTIEPIPATDSEETPMHMNRREFLQLLAVAAASGMALDSKSALAGKAPANFYDVPRHGNVSFLHF
TDCHAQLLPVWFREPNVNLGVGAAHGKAPHLVGQHLLKQFGIKPGSAEAHAFTYLDFTEAAKVYGKVGGFAHLKTLVDKL
RAQRPGALLLDGGDTWQGSATSLWTNAQDMVDACIKLGVNVMTPHWESTFGAERVLEIVNGDFKKANIDFVAQNVVTNDF
GDQVFKPYVMKDMNGVKVAVIGQAFPYTPIANPRHMVPDWSFGIRDDSMQQFVDQARAEGAKVVVVLSHNGMDVDLKMAS
RVTGIDAIFGGHTHDGVPQPTKVKNAKGVTLVTNAGSNGKFLGVMDFDVRGGKVQSYKYRLLPVFSNLLPADPGMDAFIK
QVRAPYEAKLSEKLAVTDDFLYRRGNFNGTWDQLLVDALMEVKGADAAFSPGFRWGTTLLPGDAITMERLMDQTAITYPQ
TTLTEMTGETIKTIMEDVADNLFNADPYYQQGGDMVRVGGIEYTIDPNKKIGQRIGDMKLNGKPVSADKTYMVAGWAPVG
EGVQGEPVWDVVATYLRDKKVIKGLKLNEPKIVGVGKSNPGIAAYSGGLS

Sequences:

>Translated_610_residues
MEHGQELLFTGLAGKLTIEPIPATDSEETPMHMNRREFLQLLAVAAASGMALDSKSALAGKAPANFYDVPRHGNVSFLHF
TDCHAQLLPVWFREPNVNLGVGAAHGKAPHLVGQHLLKQFGIKPGSAEAHAFTYLDFTEAAKVYGKVGGFAHLKTLVDKL
RAQRPGALLLDGGDTWQGSATSLWTNAQDMVDACIKLGVNVMTPHWESTFGAERVLEIVNGDFKKANIDFVAQNVVTNDF
GDQVFKPYVMKDMNGVKVAVIGQAFPYTPIANPRHMVPDWSFGIRDDSMQQFVDQARAEGAKVVVVLSHNGMDVDLKMAS
RVTGIDAIFGGHTHDGVPQPTKVKNAKGVTLVTNAGSNGKFLGVMDFDVRGGKVQSYKYRLLPVFSNLLPADPGMDAFIK
QVRAPYEAKLSEKLAVTDDFLYRRGNFNGTWDQLLVDALMEVKGADAAFSPGFRWGTTLLPGDAITMERLMDQTAITYPQ
TTLTEMTGETIKTIMEDVADNLFNADPYYQQGGDMVRVGGIEYTIDPNKKIGQRIGDMKLNGKPVSADKTYMVAGWAPVG
EGVQGEPVWDVVATYLRDKKVIKGLKLNEPKIVGVGKSNPGIAAYSGGLS
>Mature_610_residues
MEHGQELLFTGLAGKLTIEPIPATDSEETPMHMNRREFLQLLAVAAASGMALDSKSALAGKAPANFYDVPRHGNVSFLHF
TDCHAQLLPVWFREPNVNLGVGAAHGKAPHLVGQHLLKQFGIKPGSAEAHAFTYLDFTEAAKVYGKVGGFAHLKTLVDKL
RAQRPGALLLDGGDTWQGSATSLWTNAQDMVDACIKLGVNVMTPHWESTFGAERVLEIVNGDFKKANIDFVAQNVVTNDF
GDQVFKPYVMKDMNGVKVAVIGQAFPYTPIANPRHMVPDWSFGIRDDSMQQFVDQARAEGAKVVVVLSHNGMDVDLKMAS
RVTGIDAIFGGHTHDGVPQPTKVKNAKGVTLVTNAGSNGKFLGVMDFDVRGGKVQSYKYRLLPVFSNLLPADPGMDAFIK
QVRAPYEAKLSEKLAVTDDFLYRRGNFNGTWDQLLVDALMEVKGADAAFSPGFRWGTTLLPGDAITMERLMDQTAITYPQ
TTLTEMTGETIKTIMEDVADNLFNADPYYQQGGDMVRVGGIEYTIDPNKKIGQRIGDMKLNGKPVSADKTYMVAGWAPVG
EGVQGEPVWDVVATYLRDKKVIKGLKLNEPKIVGVGKSNPGIAAYSGGLS

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=514, Percent_Identity=23.9299610894942, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786687, Length=506, Percent_Identity=23.1225296442688, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 66265; Mature: 66265

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHGQELLFTGLAGKLTIEPIPATDSEETPMHMNRREFLQLLAVAAASGMALDSKSALAG
CCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCC
KAPANFYDVPRHGNVSFLHFTDCHAQLLPVWFREPNVNLGVGAAHGKAPHLVGQHLLKQF
CCCCCCEECCCCCCEEEEEEECCCHHEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHH
GIKPGSAEAHAFTYLDFTEAAKVYGKVGGFAHLKTLVDKLRAQRPGALLLDGGDTWQGSA
CCCCCCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
TSLWTNAQDMVDACIKLGVNVMTPHWESTFGAERVLEIVNGDFKKANIDFVAQNVVTNDF
CEEECCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCH
GDQVFKPYVMKDMNGVKVAVIGQAFPYTPIANPRHMVPDWSFGIRDDSMQQFVDQARAEG
HHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
AKVVVVLSHNGMDVDLKMASRVTGIDAIFGGHTHDGVPQPTKVKNAKGVTLVTNAGSNGK
CEEEEEEECCCCEEEEEHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
FLGVMDFDVRGGKVQSYKYRLLPVFSNLLPADPGMDAFIKQVRAPYEAKLSEKLAVTDDF
EEEEEEECCCCCEEECEEEEEEHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHH
LYRRGNFNGTWDQLLVDALMEVKGADAAFSPGFRWGTTLLPGDAITMERLMDQTAITYPQ
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCC
TTLTEMTGETIKTIMEDVADNLFNADPYYQQGGDMVRVGGIEYTIDPNKKIGQRIGDMKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCEEEEECCCHHHHHHHCCCEE
NGKPVSADKTYMVAGWAPVGEGVQGEPVWDVVATYLRDKKVIKGLKLNEPKIVGVGKSNP
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
GIAAYSGGLS
CCEEECCCCC
>Mature Secondary Structure
MEHGQELLFTGLAGKLTIEPIPATDSEETPMHMNRREFLQLLAVAAASGMALDSKSALAG
CCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCC
KAPANFYDVPRHGNVSFLHFTDCHAQLLPVWFREPNVNLGVGAAHGKAPHLVGQHLLKQF
CCCCCCEECCCCCCEEEEEEECCCHHEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHH
GIKPGSAEAHAFTYLDFTEAAKVYGKVGGFAHLKTLVDKLRAQRPGALLLDGGDTWQGSA
CCCCCCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
TSLWTNAQDMVDACIKLGVNVMTPHWESTFGAERVLEIVNGDFKKANIDFVAQNVVTNDF
CEEECCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCH
GDQVFKPYVMKDMNGVKVAVIGQAFPYTPIANPRHMVPDWSFGIRDDSMQQFVDQARAEG
HHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
AKVVVVLSHNGMDVDLKMASRVTGIDAIFGGHTHDGVPQPTKVKNAKGVTLVTNAGSNGK
CEEEEEEECCCCEEEEEHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
FLGVMDFDVRGGKVQSYKYRLLPVFSNLLPADPGMDAFIKQVRAPYEAKLSEKLAVTDDF
EEEEEEECCCCCEEECEEEEEEHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHH
LYRRGNFNGTWDQLLVDALMEVKGADAAFSPGFRWGTTLLPGDAITMERLMDQTAITYPQ
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCC
TTLTEMTGETIKTIMEDVADNLFNADPYYQQGGDMVRVGGIEYTIDPNKKIGQRIGDMKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCEEEEECCCHHHHHHHCCCEE
NGKPVSADKTYMVAGWAPVGEGVQGEPVWDVVATYLRDKKVIKGLKLNEPKIVGVGKSNP
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
GIAAYSGGLS
CCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]