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The map label for this gene is yafV [C]

Identifier: 74316529

GI number: 74316529

Start: 539914

End: 540873

Strand: Direct

Name: yafV [C]

Synonym: Tbd_0511

Alternate gene names: 74316529

Gene position: 539914-540873 (Clockwise)

Preceding gene: 74316528

Following gene: 74316530

Centisome position: 18.55

GC content: 65.42

Gene sequence:

>960_bases
ATGACGACAAAAAAGCCAGCAAAAACCACCGTCGCGCGTCCCAAAGGCGCCCAGGTCAAAAAACTCGCGCCCCAGCCGGG
CGCGGTTCGCGTCGCCGCGATCCAGATGGCTTCGGGACCGAGCGTGCCTGCCAACCTCGCCGAAGCCGAGCGGCTCATCG
AACTCGCGGTCCAGGCGGGCGCGCGTCTCGTCGTGCTGCCCGAATTCTTCTGCATCATGGCAATGAAGGACAGCTACGTC
GTGAAGGCGCGCGAGGCCGAAGGCGACGGCCCGATCCAGACCTTCCTCGCGCGCATGGCGAAGAAGCACAAGGTCTGGCT
GATCGGCGGCTCGGTCCCGCTCGAGGCGAGCGTGCCGAACAAGGTACGCAACAGCTGCCTGGTCTACGACGAGCGCGGCA
AGCAGATCGCCCGCTACGACAAGATCCATCTCTTCGGCCTCGACCTCGGCAACGAGCGCTATCAGGAAGCCAAGCTCATC
GAACCCGGAGACAAGGTCGTCGTCGTCAACAGCCCGTTCGGCCGCATCGGTCTGTCGGTCTGCTACGATCTGCGCTTCCC
CGAGCTCTATCGTGCGATGCCGGATGTCGACATCATTGTCGTGCCGTCGGCCTTTACCGCGACGACCGGACGCGCCCACT
TCGAAACACTGGTCCGCGCGCGGGCGATCGAGAACCTCGCCTATGTGATCGCCCCGGCGCAGGGCGGCTACCATCTTTCC
GGCCGCGAAACCCACGGCGACAGCATGATCGTCGACCCCTGGGGCGTCGTGCTCGACCGCCTGCCGCGCGGCTCGGGCGT
CGTCATCGCCAACATCAACCCGGCCTATCAGGCCAGCCTGCGCAAGAGCCTGCCGGCGCTGCAGCATCGCTTCATCCCCT
GTCAGCAGATAGCGGTCGATGTCGTCGAGCGGCGCGCCGTGGCCAAACGCGAGCCCACGAGCAAACAGGACAAGCCATGA

Upstream 100 bases:

>100_bases
ATGGTGACCGGGCCGTGGCAGGCGCCCGACGTCAAACGTCTCGCCAAAACCAAGGTCGAATCCAAACCCCCCGTAGCCGA
ACCCTGATGTGGAACCGTAG

Downstream 100 bases:

>100_bases
ACCCGACCGACGCCTTCGTTCCGATCCAGTCCGCCGAACAGCTGTTCCGTTCGGCCGAAGCCACCCTGCTGACGCCGAAC
GGCCTCGACGCCGACAAGCT

Product: carbon-nitrogen hydrolase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTTKKPAKTTVARPKGAQVKKLAPQPGAVRVAAIQMASGPSVPANLAEAERLIELAVQAGARLVVLPEFFCIMAMKDSYV
VKAREAEGDGPIQTFLARMAKKHKVWLIGGSVPLEASVPNKVRNSCLVYDERGKQIARYDKIHLFGLDLGNERYQEAKLI
EPGDKVVVVNSPFGRIGLSVCYDLRFPELYRAMPDVDIIVVPSAFTATTGRAHFETLVRARAIENLAYVIAPAQGGYHLS
GRETHGDSMIVDPWGVVLDRLPRGSGVVIANINPAYQASLRKSLPALQHRFIPCQQIAVDVVERRAVAKREPTSKQDKP

Sequences:

>Translated_319_residues
MTTKKPAKTTVARPKGAQVKKLAPQPGAVRVAAIQMASGPSVPANLAEAERLIELAVQAGARLVVLPEFFCIMAMKDSYV
VKAREAEGDGPIQTFLARMAKKHKVWLIGGSVPLEASVPNKVRNSCLVYDERGKQIARYDKIHLFGLDLGNERYQEAKLI
EPGDKVVVVNSPFGRIGLSVCYDLRFPELYRAMPDVDIIVVPSAFTATTGRAHFETLVRARAIENLAYVIAPAQGGYHLS
GRETHGDSMIVDPWGVVLDRLPRGSGVVIANINPAYQASLRKSLPALQHRFIPCQQIAVDVVERRAVAKREPTSKQDKP
>Mature_318_residues
TTKKPAKTTVARPKGAQVKKLAPQPGAVRVAAIQMASGPSVPANLAEAERLIELAVQAGARLVVLPEFFCIMAMKDSYVV
KAREAEGDGPIQTFLARMAKKHKVWLIGGSVPLEASVPNKVRNSCLVYDERGKQIARYDKIHLFGLDLGNERYQEAKLIE
PGDKVVVVNSPFGRIGLSVCYDLRFPELYRAMPDVDIIVVPSAFTATTGRAHFETLVRARAIENLAYVIAPAQGGYHLSG
RETHGDSMIVDPWGVVLDRLPRGSGVVIANINPAYQASLRKSLPALQHRFIPCQQIAVDVVERRAVAKREPTSKQDKP

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=267, Percent_Identity=36.7041198501873, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI5031947, Length=267, Percent_Identity=36.3295880149813, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI297632348, Length=267, Percent_Identity=36.7041198501873, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI9910460, Length=266, Percent_Identity=33.4586466165414, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=31.7708333333333, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1786412, Length=248, Percent_Identity=31.0483870967742, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=268, Percent_Identity=36.5671641791045, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323383, Length=275, Percent_Identity=32.7272727272727, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322335, Length=301, Percent_Identity=31.5614617940199, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17933642, Length=311, Percent_Identity=33.1189710610932, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355835, Length=274, Percent_Identity=30.2919708029197, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 34879; Mature: 34748

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKKPAKTTVARPKGAQVKKLAPQPGAVRVAAIQMASGPSVPANLAEAERLIELAVQAG
CCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
ARLVVLPEFFCIMAMKDSYVVKAREAEGDGPIQTFLARMAKKHKVWLIGGSVPLEASVPN
CEEEEECCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCH
KVRNSCLVYDERGKQIARYDKIHLFGLDLGNERYQEAKLIEPGDKVVVVNSPFGRIGLSV
HHHCCEEEECCCCCEEHHCCEEEEEEEECCCCHHHHCCCCCCCCCEEEEECCCCHHHHHH
CYDLRFPELYRAMPDVDIIVVPSAFTATTGRAHFETLVRARAIENLAYVIAPAQGGYHLS
EEECCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCEEEC
GRETHGDSMIVDPWGVVLDRLPRGSGVVIANINPAYQASLRKSLPALQHRFIPCQQIAVD
CCCCCCCCEEECCHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCHHHHHHCCCHHHHHHH
VVERRAVAKREPTSKQDKP
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTKKPAKTTVARPKGAQVKKLAPQPGAVRVAAIQMASGPSVPANLAEAERLIELAVQAG
CCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
ARLVVLPEFFCIMAMKDSYVVKAREAEGDGPIQTFLARMAKKHKVWLIGGSVPLEASVPN
CEEEEECCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCH
KVRNSCLVYDERGKQIARYDKIHLFGLDLGNERYQEAKLIEPGDKVVVVNSPFGRIGLSV
HHHCCEEEECCCCCEEHHCCEEEEEEEECCCCHHHHCCCCCCCCCEEEEECCCCHHHHHH
CYDLRFPELYRAMPDVDIIVVPSAFTATTGRAHFETLVRARAIENLAYVIAPAQGGYHLS
EEECCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCEEEC
GRETHGDSMIVDPWGVVLDRLPRGSGVVIANINPAYQASLRKSLPALQHRFIPCQQIAVD
CCCCCCCCEEECCHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCHHHHHHCCCHHHHHHH
VVERRAVAKREPTSKQDKP
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]