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The map label for this gene is rph [H]

Identifier: 74316495

GI number: 74316495

Start: 499637

End: 500371

Strand: Direct

Name: rph [H]

Synonym: Tbd_0477

Alternate gene names: 74316495

Gene position: 499637-500371 (Clockwise)

Preceding gene: 74316494

Following gene: 74316496

Centisome position: 17.17

GC content: 69.66

Gene sequence:

>735_bases
ATGTCCGAATTGACCGCCCGCCCCAGCGGCCGCGCGCCCGACGCGCTCCGTGCGCTGCGCTTCACCCGTCGCTTCACCAA
GCATGCCGAAGGCTCCGTGCTCGTCGCCATGGGCGATACCCAAGTCCTCTGCACGGCCAGCGTCGTCGACAAGGTCCCGC
CGCACAAGAAAGGCAGCGGCGAGGGCTGGGTCACCGCCGAATACGGCATGCTCCCGCGCTCGACCCACACGCGCAGTGAT
CGCGAGGCGGCGCGCGGCAAGCAGTCGGGGCGCACGCAGGAAATCCAGCGCCTGATCGGGCGCAGCCTGCGCGCGGTCGT
CGACCTGAAGAAGCTCGGCGAGCGCACGCTGCACATCGACTGCGACGTGCTGCAGGCCGACGGCGGCACGCGCTGCGCGA
GCATTTGCGGCGCCTACGTCGCGGTCGCCGACGCGGTCTCCTGGCTCGTGCGCGAAGGGGCGCTCGTCGAAACGCCGCTC
GTCGACAGCGTCGCCGCGGTCTCGGTCGGCGTCTACCAGGGGCAGCCCGTGCTCGATCTCGATTACCTCGAGGATTCCGC
CTGTGACACCGACATGAACGTCGTCATGACCGGAAGCGGCGGCATCGTCGAGGTGCAGGGGACGGCCGAAGGCGTGGCGT
TTTCGCGCACCACCCTCGAAGCCTTGCTCGACCTGGCCACGGCCGGCATCGCGCAGATCAGCGCGGAACAGGTCAGGACA
CTGGGGCAGCCATGA

Upstream 100 bases:

>100_bases
GGCGATCCAGAAAATCTCCGGCAAATAGCACGCGCTGGGCCGCGATCTGGCGGCCTCGCCGCGCGGTAGAATCGGTGCTT
TCGTTTCCGATTTCCGCCCC

Downstream 100 bases:

>100_bases
GCAAGATCGTCATCGCGTCCGGCAATCCCGGCAAGCTGCGCGAAATCGCGCGCATCCTCGCGCCGCTCGAGATCGAAGCG
GTGCCGCAGTCGGATTTCGA

Product: RNAse PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSELTARPSGRAPDALRALRFTRRFTKHAEGSVLVAMGDTQVLCTASVVDKVPPHKKGSGEGWVTAEYGMLPRSTHTRSD
REAARGKQSGRTQEIQRLIGRSLRAVVDLKKLGERTLHIDCDVLQADGGTRCASICGAYVAVADAVSWLVREGALVETPL
VDSVAAVSVGVYQGQPVLDLDYLEDSACDTDMNVVMTGSGGIVEVQGTAEGVAFSRTTLEALLDLATAGIAQISAEQVRT
LGQP

Sequences:

>Translated_244_residues
MSELTARPSGRAPDALRALRFTRRFTKHAEGSVLVAMGDTQVLCTASVVDKVPPHKKGSGEGWVTAEYGMLPRSTHTRSD
REAARGKQSGRTQEIQRLIGRSLRAVVDLKKLGERTLHIDCDVLQADGGTRCASICGAYVAVADAVSWLVREGALVETPL
VDSVAAVSVGVYQGQPVLDLDYLEDSACDTDMNVVMTGSGGIVEVQGTAEGVAFSRTTLEALLDLATAGIAQISAEQVRT
LGQP
>Mature_243_residues
SELTARPSGRAPDALRALRFTRRFTKHAEGSVLVAMGDTQVLCTASVVDKVPPHKKGSGEGWVTAEYGMLPRSTHTRSDR
EAARGKQSGRTQEIQRLIGRSLRAVVDLKKLGERTLHIDCDVLQADGGTRCASICGAYVAVADAVSWLVREGALVETPLV
DSVAAVSVGVYQGQPVLDLDYLEDSACDTDMNVVMTGSGGIVEVQGTAEGVAFSRTTLEALLDLATAGIAQISAEQVRTL
GQP

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=65.0717703349282, Blast_Score=275, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71981632, Length=191, Percent_Identity=26.1780104712042, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25852; Mature: 25720

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTARPSGRAPDALRALRFTRRFTKHAEGSVLVAMGDTQVLCTASVVDKVPPHKKGSG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHCCCCCCCCCC
EGWVTAEYGMLPRSTHTRSDREAARGKQSGRTQEIQRLIGRSLRAVVDLKKLGERTLHID
CCEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
CDVLQADGGTRCASICGAYVAVADAVSWLVREGALVETPLVDSVAAVSVGVYQGQPVLDL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHEEEEECCCCCEEH
DYLEDSACDTDMNVVMTGSGGIVEVQGTAEGVAFSRTTLEALLDLATAGIAQISAEQVRT
HCCCCCCCCCCCEEEEECCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQP
CCCC
>Mature Secondary Structure 
SELTARPSGRAPDALRALRFTRRFTKHAEGSVLVAMGDTQVLCTASVVDKVPPHKKGSG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHCCCCCCCCCC
EGWVTAEYGMLPRSTHTRSDREAARGKQSGRTQEIQRLIGRSLRAVVDLKKLGERTLHID
CCEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
CDVLQADGGTRCASICGAYVAVADAVSWLVREGALVETPLVDSVAAVSVGVYQGQPVLDL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHEEEEECCCCCEEH
DYLEDSACDTDMNVVMTGSGGIVEVQGTAEGVAFSRTTLEALLDLATAGIAQISAEQVRT
HCCCCCCCCCCCEEEEECCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA