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The map label for this gene is recG [H]

Identifier: 74316487

GI number: 74316487

Start: 491230

End: 493266

Strand: Reverse

Name: recG [H]

Synonym: Tbd_0469

Alternate gene names: 74316487

Gene position: 493266-491230 (Counterclockwise)

Preceding gene: 74316488

Following gene: 74316482

Centisome position: 16.95

GC content: 70.35

Gene sequence:

>2037_bases
TTGAAGACGGCTTCGCCGTTTTCCTTTCCGGTCAGTCCGGCGCTTGCGGCCAAGCTCGCCAAGCTCGGTCTCAAGCGCGA
CAGCGACCTGATCCTCCACCTGCCGCTGCGCTGGGAAGACGAAACGCGGATCACGCCGATCGGCGAACTGCTGCCGGGAC
AGACCGCGCAGGTGCAGGCCTGCGTGCGCGAAGCGAAGGTCACCTATCGTCCGCGGCGCATGCTGACGCTGATCGTCGAG
GACGACAGCGGCGTGCTCGGCGTCCGCTTCCTGCACTTCTATCCCAGCCACCTCAAGGCGTTCCAGGCCGGGGCGCGCTT
CCGCTTCAATGGCGAAGTCCGCGGCGGCTTCCTCGGTCTGGAGATGGTGCACCCGCGTTTCGCCAAGGCCGACGACGACA
CCCCGCTGCCGGCCGGGCTGACGCCGGTCTACCCGACGACCGCCGGACTCGGCCAGGCGAGCCTGCGCAAGCTGATCGAG
CGCGCGCTCGCCGCGCCCATCGATGAGACGCTGCCGCCCGAATGGCGCGAAGAACTGGGCCTCGCGGAACTGGAATCGAG
CATCCGCCTGCTGCACCAGCCGCCGCCCGGCTGCGCGCAGCATGCGCTCGAATCGCGCGCCCACCCCGCCTGGCAGCGGC
TCGCCTTCGACGAACTGCTGGCGCAGCAGCTGTCGCTGGCGACGTCCCGCCAGGCGCGCGAGGCGCGCACGACTTTGCCG
CTTCCGCACCGGCGCCGCCTGACCGCGGCCTTCGTCGACACCCTCGCTTTCGAACTGACCGCGGCGCAGGCCCGCGCCTG
GCAGGAAATCAGTACCGACCTCGCCGCGCCGCATCCGATGCGCAGGCTGCTGCAGGGCGACGTCGGCAGCGGCAAGACCG
TGGTCGCGGCGCTCGCCTGCCTGCAGGCAGTCGAGAACGGCATGCAGGCCGCGTTCATGGCGCCGACCGAGATCCTGGCC
GAGCAGCACTACCGCAAGCTCGCGCCGACGCTTGCGGCGCTCGGCGTGCGCAGCGGGTGGGTTTCGGGCAGCCAGCGCCG
TGCCGAACGCGAAGCCGCGTGGCGCGCGATCGCCGCCGGGGAGGCCGACCTCGCCTTCGGCACGCATGCCCTGTTCCAGG
AGGCCGGAAACTTTTGCCGGCTCGGCCTCGTCGTCGTCGACGAACAGCACCGCTTCGGCGTCGGCCAGCGCCTTGCGCTG
ATGGGCAAAGGCACCGAGCCGCACCAGTTGATGATGAGCGCGACGCCGATTCCACGCACGCTCGCGATGAGCTTTTTCGC
CGACCTCGACGTCTCGGCCATCGACGAACTCCCGCCCGGGCGCACGCCGATCGTGACCAAGCTGGTCAGTGCCGCGCGCC
GCGACGAGGTGCTCGCCCGTGTGCGCGACGCCTGTGTGGGCGGCGGACAGGCCTACTGGGTGTGCCCGCTGATCGAGGAA
TCGGAGAAGCTGCAGCTGCAGACCGCCGAGGACACCTACGGCACGCTCGTCGAGCAGCTGCCCGGGCTGCGCATCGGCCT
CGTGCACGGCCGGCTCAAGGCCGAGGACAAGCAATCGGTCATGGCGGCGTTCCAGTCTCACGAGATCGACCTGCTCGTCG
CGACGACCGTGATCGAGGTCGGCGTCGACGTGCCCAACGCGGCGCTCATGGTGATCGAGCACGCAGAGCGCATGGGGCTC
GCGCAATTGCATCAGTTGCGCGGGCGGGTCGGGCGCGGCGCCCGCGAATCGGTGTGCGTGCTGTTGTACGAGACGCCGCT
GTCCGAACTCGCGCGGGCGCGGCTCAAGGTGATTTTCGAACACAGCGACGGCTTCGAGATCGCGCGCCAGGATCTGCTGC
TGCGCGGACCGGGCGAGTTGCTCGGCCAGCGGCAGAGCGGTCTGCCGATGCTGCGTTTCGCCGACCTCGAGCGCGATGTC
GCCTTGCTCGAGCGCGCGCGCGACCTCGCGCAACGCTGCCTGCGCGAGCGCCCCGAGGTCGCCGCGCGCCACGTCGAGCG
CTGGCTCGGAAGCCGCCAGGCCTACAGCGGAAGCTGA

Upstream 100 bases:

>100_bases
GGCCCCCTACCCGGCGCGGGCTGCGGTCGGCGTCGCTGCCCTGCCGCGCGGCGCACTGGTCGAGGCCGACGCCGTCATGG
CCCTTGCCTAGATCGCGCGC

Downstream 100 bases:

>100_bases
GCCGCCGCACCGTGCATGCCTTGATTCCAGAGGCTCAGCGCGGGCACGTAGGTGAGGAGTAGCAGCCAGACGAGCAGCAG
CAGCAGGAACGGCAGCGTCG

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MKTASPFSFPVSPALAAKLAKLGLKRDSDLILHLPLRWEDETRITPIGELLPGQTAQVQACVREAKVTYRPRRMLTLIVE
DDSGVLGVRFLHFYPSHLKAFQAGARFRFNGEVRGGFLGLEMVHPRFAKADDDTPLPAGLTPVYPTTAGLGQASLRKLIE
RALAAPIDETLPPEWREELGLAELESSIRLLHQPPPGCAQHALESRAHPAWQRLAFDELLAQQLSLATSRQAREARTTLP
LPHRRRLTAAFVDTLAFELTAAQARAWQEISTDLAAPHPMRRLLQGDVGSGKTVVAALACLQAVENGMQAAFMAPTEILA
EQHYRKLAPTLAALGVRSGWVSGSQRRAEREAAWRAIAAGEADLAFGTHALFQEAGNFCRLGLVVVDEQHRFGVGQRLAL
MGKGTEPHQLMMSATPIPRTLAMSFFADLDVSAIDELPPGRTPIVTKLVSAARRDEVLARVRDACVGGGQAYWVCPLIEE
SEKLQLQTAEDTYGTLVEQLPGLRIGLVHGRLKAEDKQSVMAAFQSHEIDLLVATTVIEVGVDVPNAALMVIEHAERMGL
AQLHQLRGRVGRGARESVCVLLYETPLSELARARLKVIFEHSDGFEIARQDLLLRGPGELLGQRQSGLPMLRFADLERDV
ALLERARDLAQRCLRERPEVAARHVERWLGSRQAYSGS

Sequences:

>Translated_678_residues
MKTASPFSFPVSPALAAKLAKLGLKRDSDLILHLPLRWEDETRITPIGELLPGQTAQVQACVREAKVTYRPRRMLTLIVE
DDSGVLGVRFLHFYPSHLKAFQAGARFRFNGEVRGGFLGLEMVHPRFAKADDDTPLPAGLTPVYPTTAGLGQASLRKLIE
RALAAPIDETLPPEWREELGLAELESSIRLLHQPPPGCAQHALESRAHPAWQRLAFDELLAQQLSLATSRQAREARTTLP
LPHRRRLTAAFVDTLAFELTAAQARAWQEISTDLAAPHPMRRLLQGDVGSGKTVVAALACLQAVENGMQAAFMAPTEILA
EQHYRKLAPTLAALGVRSGWVSGSQRRAEREAAWRAIAAGEADLAFGTHALFQEAGNFCRLGLVVVDEQHRFGVGQRLAL
MGKGTEPHQLMMSATPIPRTLAMSFFADLDVSAIDELPPGRTPIVTKLVSAARRDEVLARVRDACVGGGQAYWVCPLIEE
SEKLQLQTAEDTYGTLVEQLPGLRIGLVHGRLKAEDKQSVMAAFQSHEIDLLVATTVIEVGVDVPNAALMVIEHAERMGL
AQLHQLRGRVGRGARESVCVLLYETPLSELARARLKVIFEHSDGFEIARQDLLLRGPGELLGQRQSGLPMLRFADLERDV
ALLERARDLAQRCLRERPEVAARHVERWLGSRQAYSGS
>Mature_678_residues
MKTASPFSFPVSPALAAKLAKLGLKRDSDLILHLPLRWEDETRITPIGELLPGQTAQVQACVREAKVTYRPRRMLTLIVE
DDSGVLGVRFLHFYPSHLKAFQAGARFRFNGEVRGGFLGLEMVHPRFAKADDDTPLPAGLTPVYPTTAGLGQASLRKLIE
RALAAPIDETLPPEWREELGLAELESSIRLLHQPPPGCAQHALESRAHPAWQRLAFDELLAQQLSLATSRQAREARTTLP
LPHRRRLTAAFVDTLAFELTAAQARAWQEISTDLAAPHPMRRLLQGDVGSGKTVVAALACLQAVENGMQAAFMAPTEILA
EQHYRKLAPTLAALGVRSGWVSGSQRRAEREAAWRAIAAGEADLAFGTHALFQEAGNFCRLGLVVVDEQHRFGVGQRLAL
MGKGTEPHQLMMSATPIPRTLAMSFFADLDVSAIDELPPGRTPIVTKLVSAARRDEVLARVRDACVGGGQAYWVCPLIEE
SEKLQLQTAEDTYGTLVEQLPGLRIGLVHGRLKAEDKQSVMAAFQSHEIDLLVATTVIEVGVDVPNAALMVIEHAERMGL
AQLHQLRGRVGRGARESVCVLLYETPLSELARARLKVIFEHSDGFEIARQDLLLRGPGELLGQRQSGLPMLRFADLERDV
ALLERARDLAQRCLRERPEVAARHVERWLGSRQAYSGS

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=679, Percent_Identity=50.9572901325479, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1787357, Length=454, Percent_Identity=35.9030837004405, Blast_Score=240, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 74528; Mature: 74528

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTASPFSFPVSPALAAKLAKLGLKRDSDLILHLPLRWEDETRITPIGELLPGQTAQVQA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHH
CVREAKVTYRPRRMLTLIVEDDSGVLGVRFLHFYPSHLKAFQAGARFRFNGEVRGGFLGL
HHHHHHCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEH
EMVHPRFAKADDDTPLPAGLTPVYPTTAGLGQASLRKLIERALAAPIDETLPPEWREELG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
LAELESSIRLLHQPPPGCAQHALESRAHPAWQRLAFDELLAQQLSLATSRQAREARTTLP
HHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPHRRRLTAAFVDTLAFELTAAQARAWQEISTDLAAPHPMRRLLQGDVGSGKTVVAALAC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHH
LQAVENGMQAAFMAPTEILAEQHYRKLAPTLAALGVRSGWVSGSQRRAEREAAWRAIAAG
HHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
EADLAFGTHALFQEAGNFCRLGLVVVDEQHRFGVGQRLALMGKGTEPHQLMMSATPIPRT
CCHHHHHHHHHHHHCCCEEEEEEEEECCCHHCCCCCEEEEECCCCCCHHHHEECCCCCHH
LAMSFFADLDVSAIDELPPGRTPIVTKLVSAARRDEVLARVRDACVGGGQAYWVCPLIEE
HHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
SEKLQLQTAEDTYGTLVEQLPGLRIGLVHGRLKAEDKQSVMAAFQSHEIDLLVATTVIEV
CCCEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHH
GVDVPNAALMVIEHAERMGLAQLHQLRGRVGRGARESVCVLLYETPLSELARARLKVIFE
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHEEEE
HSDGFEIARQDLLLRGPGELLGQRQSGLPMLRFADLERDVALLERARDLAQRCLRERPEV
CCCCHHHHHHHHEEECCHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHH
AARHVERWLGSRQAYSGS
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKTASPFSFPVSPALAAKLAKLGLKRDSDLILHLPLRWEDETRITPIGELLPGQTAQVQA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHH
CVREAKVTYRPRRMLTLIVEDDSGVLGVRFLHFYPSHLKAFQAGARFRFNGEVRGGFLGL
HHHHHHCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEH
EMVHPRFAKADDDTPLPAGLTPVYPTTAGLGQASLRKLIERALAAPIDETLPPEWREELG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
LAELESSIRLLHQPPPGCAQHALESRAHPAWQRLAFDELLAQQLSLATSRQAREARTTLP
HHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPHRRRLTAAFVDTLAFELTAAQARAWQEISTDLAAPHPMRRLLQGDVGSGKTVVAALAC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHH
LQAVENGMQAAFMAPTEILAEQHYRKLAPTLAALGVRSGWVSGSQRRAEREAAWRAIAAG
HHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
EADLAFGTHALFQEAGNFCRLGLVVVDEQHRFGVGQRLALMGKGTEPHQLMMSATPIPRT
CCHHHHHHHHHHHHCCCEEEEEEEEECCCHHCCCCCEEEEECCCCCCHHHHEECCCCCHH
LAMSFFADLDVSAIDELPPGRTPIVTKLVSAARRDEVLARVRDACVGGGQAYWVCPLIEE
HHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
SEKLQLQTAEDTYGTLVEQLPGLRIGLVHGRLKAEDKQSVMAAFQSHEIDLLVATTVIEV
CCCEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHH
GVDVPNAALMVIEHAERMGLAQLHQLRGRVGRGARESVCVLLYETPLSELARARLKVIFE
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHEEEE
HSDGFEIARQDLLLRGPGELLGQRQSGLPMLRFADLERDVALLERARDLAQRCLRERPEV
CCCCHHHHHHHHEEECCHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHH
AARHVERWLGSRQAYSGS
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]