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The map label for this gene is siaT [H]

Identifier: 74316486

GI number: 74316486

Start: 489968

End: 491245

Strand: Direct

Name: siaT [H]

Synonym: Tbd_0468

Alternate gene names: 74316486

Gene position: 489968-491245 (Clockwise)

Preceding gene: 74316485

Following gene: 74316493

Centisome position: 16.84

GC content: 67.37

Gene sequence:

>1278_bases
ATGACGCTGTTGCCGTTCGTGGCGCTGGCCTTGCTCGGCGTGCCGCTCTTCGTCGTGCTGGGCGCGGCGGCGCTCGTCGC
GACCCGCGATGCGCAACTCGATTCCGCGCTCCTGATGGTCGAGTTCGCGCGGCTCGCCGCATCACCCAATCTGGTCGCGA
TTCCGCTCTTCGTGCTCGCCGGCGCGACCATGGGGCACGGCGGCGCCGGGCGCCGCCTGGTCCGCTTCTTCAACGCCTTG
CTCGGATGGATGCCGGCGGGCATCGCCTGGGTTGCCGCCGCGTCGTGCATGCTCTTCACCGCTTTTTCCGGCGCGTCCGG
CGTCACGATCCTCGCGCTCGGCGGTCTGCTGTATCCGATGCTCAAGGACGAGGGTTACCCGGAACGCTTCGCGCTCGGGC
TCATCAGTTCGGGCGGCTCCATGGGGCTGCTGCTGCCGCCCAGCCTCGCGGTGCTGCTCTACGGCGTCGTTGCCCAGGTC
GATATCGGCGATTTGTTCGTCGCCGGCCTGCTGCCGACGCTGCTGCTGTTCGTGATGGTCGGGAGTTATGCCGTCCGGCG
GGGCGCGCGCGGGGCGCCGCGTCACCGCTTCCGATGGCGCGAACTCGCGGCCGCGGCGCGCGCGGGCTGGGCCGATCTGC
TGCTGCCGATCGGCGTCGTTGCGGGCCTGGCTGCCGGGCTGTTGACCGTCGCCGAACTCGCCGCATGCAGTGCGCTCTAC
GCGCTCGTGCTCGAAACAGTGGTTCACCGCACGATCCGCGCGAAAGAACTCGCGTGGGTGGGCTACGAAGCGGCGACGCT
GGTCGGCAGCATCTTCGTCATACTCGGCTTGAGCCTCGGACTGACCAATCTGCTGGTCGATGCCCAGGTGCCGATGCGCT
TGCTCGATTGGGTCGTCGAGCAGGTGCACAGCCCGCTCGCTTTCCTGCTCTGGATGAACGCCGTCCTGCTCGTGGTGGGC
TGCATGATGGATATCTTTTCCGCGATCGTGATCGTCACGCCGCTGCTCCTGCCGCTCGCGACCCATTTCGGGATCCACCC
GCTGCACCTCGGCATCGTGGTTCTGGCGAACCTCGAGATCGGCTATCTGACACCGCCCGTGGGCATCAACCTCTACCTTG
CCAGCCAGCGCTTCAAACAACCCGTGCTCACGGTGTTCCGTGCGACGCTGCCGTTCCTGCTGCTGCTGCTCGTCTGGCTG
CTACTCCTCACCTACGTGCCCGCGCTGAGCCTCTGGAATCAAGGCATGCACGGTGCGGCGGCTCAGCTTCCGCTGTAG

Upstream 100 bases:

>100_bases
CCTGGCTGGCCTGGCTGAGCCTCATCCTGCCCGCTGCGTTCGCGCTGCTGACGCTGCATTTTCTGCTGCGGGCCGTCCTC
GCCCGCGACGTGAAGACGCC

Downstream 100 bases:

>100_bases
GCCTGGCGGCTTCCGAGCCAGCGCTCGACGTGGCGCGCGGCGACCTCGGGGCGCTCGCGCAGGCAGCGTTGCGCGAGGTC
GCGCGCGCGCTCGAGCAAGG

Product: TRAP dicarboxylate transporter subunit DctM

Products: NA

Alternate protein names: N-acetylneuraminic acid permease; N-acetylneuraminic acid transporter; Neu5Ac permease [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTLLPFVALALLGVPLFVVLGAAALVATRDAQLDSALLMVEFARLAASPNLVAIPLFVLAGATMGHGGAGRRLVRFFNAL
LGWMPAGIAWVAAASCMLFTAFSGASGVTILALGGLLYPMLKDEGYPERFALGLISSGGSMGLLLPPSLAVLLYGVVAQV
DIGDLFVAGLLPTLLLFVMVGSYAVRRGARGAPRHRFRWRELAAAARAGWADLLLPIGVVAGLAAGLLTVAELAACSALY
ALVLETVVHRTIRAKELAWVGYEAATLVGSIFVILGLSLGLTNLLVDAQVPMRLLDWVVEQVHSPLAFLLWMNAVLLVVG
CMMDIFSAIVIVTPLLLPLATHFGIHPLHLGIVVLANLEIGYLTPPVGINLYLASQRFKQPVLTVFRATLPFLLLLLVWL
LLLTYVPALSLWNQGMHGAAAQLPL

Sequences:

>Translated_425_residues
MTLLPFVALALLGVPLFVVLGAAALVATRDAQLDSALLMVEFARLAASPNLVAIPLFVLAGATMGHGGAGRRLVRFFNAL
LGWMPAGIAWVAAASCMLFTAFSGASGVTILALGGLLYPMLKDEGYPERFALGLISSGGSMGLLLPPSLAVLLYGVVAQV
DIGDLFVAGLLPTLLLFVMVGSYAVRRGARGAPRHRFRWRELAAAARAGWADLLLPIGVVAGLAAGLLTVAELAACSALY
ALVLETVVHRTIRAKELAWVGYEAATLVGSIFVILGLSLGLTNLLVDAQVPMRLLDWVVEQVHSPLAFLLWMNAVLLVVG
CMMDIFSAIVIVTPLLLPLATHFGIHPLHLGIVVLANLEIGYLTPPVGINLYLASQRFKQPVLTVFRATLPFLLLLLVWL
LLLTYVPALSLWNQGMHGAAAQLPL
>Mature_424_residues
TLLPFVALALLGVPLFVVLGAAALVATRDAQLDSALLMVEFARLAASPNLVAIPLFVLAGATMGHGGAGRRLVRFFNALL
GWMPAGIAWVAAASCMLFTAFSGASGVTILALGGLLYPMLKDEGYPERFALGLISSGGSMGLLLPPSLAVLLYGVVAQVD
IGDLFVAGLLPTLLLFVMVGSYAVRRGARGAPRHRFRWRELAAAARAGWADLLLPIGVVAGLAAGLLTVAELAACSALYA
LVLETVVHRTIRAKELAWVGYEAATLVGSIFVILGLSLGLTNLLVDAQVPMRLLDWVVEQVHSPLAFLLWMNAVLLVVGC
MMDIFSAIVIVTPLLLPLATHFGIHPLHLGIVVLANLEIGYLTPPVGINLYLASQRFKQPVLTVFRATLPFLLLLLVWLL
LLTYVPALSLWNQGMHGAAAQLPL

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid [H]

COG id: COG1593

COG function: function code G; TRAP-type C4-dicarboxylate transport system, large permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the TRAP transporter large permease family [H]

Homologues:

Organism=Escherichia coli, GI48994950, Length=366, Percent_Identity=30.8743169398907, Blast_Score=148, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010656
- InterPro:   IPR007387
- InterPro:   IPR004681 [H]

Pfam domain/function: PF06808 DctM; PF04290 DctQ [H]

EC number: NA

Molecular weight: Translated: 45024; Mature: 44893

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLPFVALALLGVPLFVVLGAAALVATRDAQLDSALLMVEFARLAASPNLVAIPLFVLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GATMGHGGAGRRLVRFFNALLGWMPAGIAWVAAASCMLFTAFSGASGVTILALGGLLYPM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LKDEGYPERFALGLISSGGSMGLLLPPSLAVLLYGVVAQVDIGDLFVAGLLPTLLLFVMV
HCCCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GSYAVRRGARGAPRHRFRWRELAAAARAGWADLLLPIGVVAGLAAGLLTVAELAACSALY
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVLETVVHRTIRAKELAWVGYEAATLVGSIFVILGLSLGLTNLLVDAQVPMRLLDWVVE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QVHSPLAFLLWMNAVLLVVGCMMDIFSAIVIVTPLLLPLATHFGIHPLHLGIVVLANLEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
GYLTPPVGINLYLASQRFKQPVLTVFRATLPFLLLLLVWLLLLTYVPALSLWNQGMHGAA
CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AQLPL
CCCCC
>Mature Secondary Structure 
TLLPFVALALLGVPLFVVLGAAALVATRDAQLDSALLMVEFARLAASPNLVAIPLFVLA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GATMGHGGAGRRLVRFFNALLGWMPAGIAWVAAASCMLFTAFSGASGVTILALGGLLYPM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LKDEGYPERFALGLISSGGSMGLLLPPSLAVLLYGVVAQVDIGDLFVAGLLPTLLLFVMV
HCCCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GSYAVRRGARGAPRHRFRWRELAAAARAGWADLLLPIGVVAGLAAGLLTVAELAACSALY
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVLETVVHRTIRAKELAWVGYEAATLVGSIFVILGLSLGLTNLLVDAQVPMRLLDWVVE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QVHSPLAFLLWMNAVLLVVGCMMDIFSAIVIVTPLLLPLATHFGIHPLHLGIVVLANLEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
GYLTPPVGINLYLASQRFKQPVLTVFRATLPFLLLLLVWLLLLTYVPALSLWNQGMHGAA
CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AQLPL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]