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The map label for this gene is ypfJ [H]

Identifier: 74316465

GI number: 74316465

Start: 467137

End: 467985

Strand: Reverse

Name: ypfJ [H]

Synonym: Tbd_0447

Alternate gene names: 74316465

Gene position: 467985-467137 (Counterclockwise)

Preceding gene: 74316469

Following gene: 74316454

Centisome position: 16.08

GC content: 68.79

Gene sequence:

>849_bases
ATGCGCTGGGAACGCGGACGACGCAGCAGCAACATCGAAGATCGGCGCGGCATGCGGATCGGCGGCCGCGGGCTGGCCGG
CGGCGGGATCGGCGCGATCGTGCTCGCGCTCGTCGCGATGTATTTCGGCGTCGACCCGTCGGTGGTGCTCAACCAGGTGG
GCGGGCCGGCGGCGACCGAGCAGCAGGTCGCGCAGTCGCCCGAGGAGGAGCGACTCAAGGAATTCATGTCCGTCGTGCTC
GCCGACACGGAGGATGTGTGGGGCACGTTGTTCCAGCAGTCGGGCCAGCCATATCGGCAACCGACGCTGGTGCTGTTTTC
GGGTGCGGTGCAGTCGGCGTGCGGGTTTGCCGAAGCGGCGATGGGGCCTTTTTACTGCCCGGGCGACCAGAAGCTCTATC
TCGACATGAGCTTCTTCGACGATCTCGCGCAGCGCCACGACGCACCGGGCGATTTCGCCCAGGCCTACGTCGTCGCCCAC
GAGGTCGGCCACCACGTGCAGACGCTGCTCGGCGTCTCGCAGCAGGTACACGCCGCGCGTCAGCGCTCCGGCGAGGCCGC
GGGCAACGCGATGCAGGTGCGCATGGAATTGCAGGCCGACTGTTTCGCCGGCGTCTGGGCGCATCACGCCGACAAGACGC
GTCAGGTGCTCGAACCGGGTGATGCGGAAGAGGCGCTGGCCGCTGCGGCCGGGGTCGGCGACGACCGCCTGCAACGGCAG
ACGCGTGGCACGGTCGTGCCCGAATCCTTCACGCACGGCGCGTCGGAACAGCGCATGCGCTGGTTCGCGCGCGGCATGCA
GACAGGCGACCCGGGGCAATGCGATACATTCGAGGCGGCGAAGCTCTGA

Upstream 100 bases:

>100_bases
AGCGGGCCCCCGCGGGCTAGGCCCGTCGCCATTCCGGCGACTGCGCCGCTCCCGGGGTGCTGGCTACAATGAAGCGGGTG
TACCTCTTGAGGAAACCGCC

Downstream 100 bases:

>100_bases
CGCGGCTTGCGCCATGCTGGCGACGCTGCTGCTACAGCGCCTCGCCCCCGACGCAACCCGGCTGGGACGACAGCGTTACA
GCCAGCGCCCGAGCCGCGCG

Product: putative neutral zinc metallopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRWERGRRSSNIEDRRGMRIGGRGLAGGGIGAIVLALVAMYFGVDPSVVLNQVGGPAATEQQVAQSPEEERLKEFMSVVL
ADTEDVWGTLFQQSGQPYRQPTLVLFSGAVQSACGFAEAAMGPFYCPGDQKLYLDMSFFDDLAQRHDAPGDFAQAYVVAH
EVGHHVQTLLGVSQQVHAARQRSGEAAGNAMQVRMELQADCFAGVWAHHADKTRQVLEPGDAEEALAAAAGVGDDRLQRQ
TRGTVVPESFTHGASEQRMRWFARGMQTGDPGQCDTFEAAKL

Sequences:

>Translated_282_residues
MRWERGRRSSNIEDRRGMRIGGRGLAGGGIGAIVLALVAMYFGVDPSVVLNQVGGPAATEQQVAQSPEEERLKEFMSVVL
ADTEDVWGTLFQQSGQPYRQPTLVLFSGAVQSACGFAEAAMGPFYCPGDQKLYLDMSFFDDLAQRHDAPGDFAQAYVVAH
EVGHHVQTLLGVSQQVHAARQRSGEAAGNAMQVRMELQADCFAGVWAHHADKTRQVLEPGDAEEALAAAAGVGDDRLQRQ
TRGTVVPESFTHGASEQRMRWFARGMQTGDPGQCDTFEAAKL
>Mature_282_residues
MRWERGRRSSNIEDRRGMRIGGRGLAGGGIGAIVLALVAMYFGVDPSVVLNQVGGPAATEQQVAQSPEEERLKEFMSVVL
ADTEDVWGTLFQQSGQPYRQPTLVLFSGAVQSACGFAEAAMGPFYCPGDQKLYLDMSFFDDLAQRHDAPGDFAQAYVVAH
EVGHHVQTLLGVSQQVHAARQRSGEAAGNAMQVRMELQADCFAGVWAHHADKTRQVLEPGDAEEALAAAAGVGDDRLQRQ
TRGTVVPESFTHGASEQRMRWFARGMQTGDPGQCDTFEAAKL

Specific function: Unknown

COG id: COG2321

COG function: function code R; Predicted metalloprotease

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788819, Length=286, Percent_Identity=48.951048951049, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007343 [H]

Pfam domain/function: PF04228 Zn_peptidase [H]

EC number: NA

Molecular weight: Translated: 30555; Mature: 30555

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWERGRRSSNIEDRRGMRIGGRGLAGGGIGAIVLALVAMYFGVDPSVVLNQVGGPAATE
CCCCCCCCCCCCCHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHH
QQVAQSPEEERLKEFMSVVLADTEDVWGTLFQQSGQPYRQPTLVLFSGAVQSACGFAEAA
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
MGPFYCPGDQKLYLDMSFFDDLAQRHDAPGDFAQAYVVAHEVGHHVQTLLGVSQQVHAAR
CCCEECCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRSGEAAGNAMQVRMELQADCFAGVWAHHADKTRQVLEPGDAEEALAAAAGVGDDRLQRQ
HHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHH
TRGTVVPESFTHGASEQRMRWFARGMQTGDPGQCDTFEAAKL
HCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MRWERGRRSSNIEDRRGMRIGGRGLAGGGIGAIVLALVAMYFGVDPSVVLNQVGGPAATE
CCCCCCCCCCCCCHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHH
QQVAQSPEEERLKEFMSVVLADTEDVWGTLFQQSGQPYRQPTLVLFSGAVQSACGFAEAA
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
MGPFYCPGDQKLYLDMSFFDDLAQRHDAPGDFAQAYVVAHEVGHHVQTLLGVSQQVHAAR
CCCEECCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRSGEAAGNAMQVRMELQADCFAGVWAHHADKTRQVLEPGDAEEALAAAAGVGDDRLQRQ
HHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHH
TRGTVVPESFTHGASEQRMRWFARGMQTGDPGQCDTFEAAKL
HCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]