Click here to switch to the map view.

The map label for this gene is rluF [H]

Identifier: 74316453

GI number: 74316453

Start: 453878

End: 454615

Strand: Reverse

Name: rluF [H]

Synonym: Tbd_0435

Alternate gene names: 74316453

Gene position: 454615-453878 (Counterclockwise)

Preceding gene: 74316454

Following gene: 74316452

Centisome position: 15.62

GC content: 69.51

Gene sequence:

>738_bases
ATGGCCGAGCCGGTCCGTCTGTCCAAGCTCATGTCGGAGCGCGGCCTGTGCTCGCGGCGCGAGGCCGACGCCTTCATCGA
ACGCGGGCTCGTGTTCGTCGACGGCAAGCGGGTCAGCGAACTCGGGACCAAGGTCGATCCGGCCTGCGCAATCCGGCTCG
ACAACGCAGCCAGCCGTCAGCAGCAGCAACGCGTGACGATCCTGCTGCACAAACCGGTCGGCTACGTCTCAGGGCAGCCC
GAGCCGGGCTACCAGCCGGCAGTCGCGCTGATCCGCGGCGAGACGCAGTGGCGAGACGAACGGCGCCCGGGGCCGGCCTT
CCTGCCCGCGCACCTGAAGGGCCTCGCGCCGGCCGGCCGCCTTGACATCGATTCGACCGGCCTGCTCGTCCTGACCCAGG
ACGGCCGCGTCGCGCGGCAACTGATCGGCGACCACTCCGACGTCGAAAAGGAATACCTCGTACGGGTCGAGGGGCGGCTC
GACGCGCGCGGTCTCGCACTCCTCAACCACGGCCTCAGCCTCGACGGCGTTCGGCTGCGGCCGGCGAAGGTCGCCTGGCA
AAACGCCGACCAGCTTCGCTTCAGCCTCAAGGAAGGCCGCAAGCGCCAGATCCGTCGCATGTGCGAACTCGTTGGGCTCA
GGGTCGTCGGCCTGAAGCGCGTTCGCATCGGTGCAGTGCGTCTCGGCGACCTGCCGCTCGGCCGCTGGCGTTATCTGGCG
CCGGGCGAGCATTTCTAG

Upstream 100 bases:

>100_bases
GGCGGCCTCATCATCGCCGAGGGCCCGCCCGAAGCCGTCGCGGACAACCCGGCGAGCCACACCGGGCAGTATCTTCAACC
GGTGCTGTCGAGGCGCTGAG

Downstream 100 bases:

>100_bases
CACGCCGCCAGCAGCCTCGCGAGCGCTTGTTTTAGGCGGCCGTACCCGCTATGAATAAAAGCTCCGTGCGTCGCGGCCCC
GCATCATTTTCCATCCGACT

Product: ribosomal large subunit pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MAEPVRLSKLMSERGLCSRREADAFIERGLVFVDGKRVSELGTKVDPACAIRLDNAASRQQQQRVTILLHKPVGYVSGQP
EPGYQPAVALIRGETQWRDERRPGPAFLPAHLKGLAPAGRLDIDSTGLLVLTQDGRVARQLIGDHSDVEKEYLVRVEGRL
DARGLALLNHGLSLDGVRLRPAKVAWQNADQLRFSLKEGRKRQIRRMCELVGLRVVGLKRVRIGAVRLGDLPLGRWRYLA
PGEHF

Sequences:

>Translated_245_residues
MAEPVRLSKLMSERGLCSRREADAFIERGLVFVDGKRVSELGTKVDPACAIRLDNAASRQQQQRVTILLHKPVGYVSGQP
EPGYQPAVALIRGETQWRDERRPGPAFLPAHLKGLAPAGRLDIDSTGLLVLTQDGRVARQLIGDHSDVEKEYLVRVEGRL
DARGLALLNHGLSLDGVRLRPAKVAWQNADQLRFSLKEGRKRQIRRMCELVGLRVVGLKRVRIGAVRLGDLPLGRWRYLA
PGEHF
>Mature_244_residues
AEPVRLSKLMSERGLCSRREADAFIERGLVFVDGKRVSELGTKVDPACAIRLDNAASRQQQQRVTILLHKPVGYVSGQPE
PGYQPAVALIRGETQWRDERRPGPAFLPAHLKGLAPAGRLDIDSTGLLVLTQDGRVARQLIGDHSDVEKEYLVRVEGRLD
ARGLALLNHGLSLDGVRLRPAKVAWQNADQLRFSLKEGRKRQIRRMCELVGLRVVGLKRVRIGAVRLGDLPLGRWRYLAP
GEHF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=36.9294605809129, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788510, Length=242, Percent_Identity=30.1652892561983, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=32.7935222672065, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081838, Length=163, Percent_Identity=34.9693251533742, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27325; Mature: 27194

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPVRLSKLMSERGLCSRREADAFIERGLVFVDGKRVSELGTKVDPACAIRLDNAASRQ
CCCCHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCHHHHCCCCCCCEEEEEECCHHHHH
QQQRVTILLHKPVGYVSGQPEPGYQPAVALIRGETQWRDERRPGPAFLPAHLKGLAPAGR
HHCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCHHHHHCCCCCCC
LDIDSTGLLVLTQDGRVARQLIGDHSDVEKEYLVRVEGRLDARGLALLNHGLSLDGVRLR
CEECCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEECCCCCCCHHHHHHCCCCCCCEEEC
PAKVAWQNADQLRFSLKEGRKRQIRRMCELVGLRVVGLKRVRIGAVRLGDLPLGRWRYLA
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEEEECCCCCCCEEECC
PGEHF
CCCCC
>Mature Secondary Structure 
AEPVRLSKLMSERGLCSRREADAFIERGLVFVDGKRVSELGTKVDPACAIRLDNAASRQ
CCCHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCHHHHCCCCCCCEEEEEECCHHHHH
QQQRVTILLHKPVGYVSGQPEPGYQPAVALIRGETQWRDERRPGPAFLPAHLKGLAPAGR
HHCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCHHHHHCCCCCCC
LDIDSTGLLVLTQDGRVARQLIGDHSDVEKEYLVRVEGRLDARGLALLNHGLSLDGVRLR
CEECCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEECCCCCCCHHHHHHCCCCCCCEEEC
PAKVAWQNADQLRFSLKEGRKRQIRRMCELVGLRVVGLKRVRIGAVRLGDLPLGRWRYLA
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEEEECCCCCCCEEECC
PGEHF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]