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The map label for this gene is mutM

Identifier: 74316401

GI number: 74316401

Start: 411196

End: 412008

Strand: Reverse

Name: mutM

Synonym: Tbd_0383

Alternate gene names: 74316401

Gene position: 412008-411196 (Counterclockwise)

Preceding gene: 74316451

Following gene: 74316400

Centisome position: 14.16

GC content: 69.5

Gene sequence:

>813_bases
ATGCCTGAACTACCCGAAGTCGAGACCACCCGCGCCGGCCTGTCGCCCCGTTTGCAGGGTCGCGTGCTGACCCGCGTCAT
CGTTCGCGAGCCGCGTCTGCGCTGGCCGATCCCGCCCGACCTCGACACGCAATTGCGGGGCCTCACGCTCCACGGCCTCG
CCCGGCGCGGCAAATACCTGCTGTTCGACTTCGGCGCTGTCACCCAGCTCGTCCATCTCGGCATGTCGGGGAGCCTGCGC
CTCACCGAGCCCGCGGAGCCCGCTGCGCGCCACGACCACGTCGACTGGCGCTTCGACGACGGAACGATCCTGCGCCTGCG
CGACCCGCGCCGCTTCGGCGCGGTGCTGCTGACCGAGAACGCGCCCGGACATCCGCTCCTCGCCGGCCTCGGTCCGGAAC
CCCTGAGCACGGCGTTCGACGCGGCCTATCTGCACACGCAGTGCCAGCGTCGCAAGACCGCGATCAAGCCGCTCATCATG
GATGCCCATGTCGTCGTCGGCGTCGGTAACATCTACGCTTCGGAATCCTTGTTCCACGCCGGAATCCGCCCGGGCGTCGC
CGCCCACCGCCTAAGCCGGGCGGCGTGCGCGCGGCTGGCCGACGCGATCAAACAGGTGTTGACTGCGGCCATCGCAGCAG
GCGGCAGTTCGCTGCGGGACTACGTCCAAAGCAGCGGCGAACTGGGGTATTTCCAGTTGCAGACGCGGGTCTACGACCGC
GAAGACGCGCCGTGCAGGCGGTGCGCAACGCCGATCCGCCGCATCGTCCAGGCGCAGCGTGCGAGCTTCTACTGCCCGAC
GTGCCAGCGCTGA

Upstream 100 bases:

>100_bases
ACGCGACCGACGCTTCCAGAACGCTTGCCCGCCGCGTGCCGGATACAATAGCGGGCGCCGAGCCCTCCGGCTTCCGGCGC
ACCCTGCCCGCCCGCCTTGT

Downstream 100 bases:

>100_bases
TGGCCGGCAAATCAAGTTTGATCCCGGCGTGTCGTTAACCTCCTGTCCTGAGCGCGGCCGATCCGCCTGATGAAATCCAC
GACCCTCGTTGACGAGTTCG

Product: DNA-(apurinic or apyrimidinic site) lyase / formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPELPEVETTRAGLSPRLQGRVLTRVIVREPRLRWPIPPDLDTQLRGLTLHGLARRGKYLLFDFGAVTQLVHLGMSGSLR
LTEPAEPAARHDHVDWRFDDGTILRLRDPRRFGAVLLTENAPGHPLLAGLGPEPLSTAFDAAYLHTQCQRRKTAIKPLIM
DAHVVVGVGNIYASESLFHAGIRPGVAAHRLSRAACARLADAIKQVLTAAIAAGGSSLRDYVQSSGELGYFQLQTRVYDR
EDAPCRRCATPIRRIVQAQRASFYCPTCQR

Sequences:

>Translated_270_residues
MPELPEVETTRAGLSPRLQGRVLTRVIVREPRLRWPIPPDLDTQLRGLTLHGLARRGKYLLFDFGAVTQLVHLGMSGSLR
LTEPAEPAARHDHVDWRFDDGTILRLRDPRRFGAVLLTENAPGHPLLAGLGPEPLSTAFDAAYLHTQCQRRKTAIKPLIM
DAHVVVGVGNIYASESLFHAGIRPGVAAHRLSRAACARLADAIKQVLTAAIAAGGSSLRDYVQSSGELGYFQLQTRVYDR
EDAPCRRCATPIRRIVQAQRASFYCPTCQR
>Mature_269_residues
PELPEVETTRAGLSPRLQGRVLTRVIVREPRLRWPIPPDLDTQLRGLTLHGLARRGKYLLFDFGAVTQLVHLGMSGSLRL
TEPAEPAARHDHVDWRFDDGTILRLRDPRRFGAVLLTENAPGHPLLAGLGPEPLSTAFDAAYLHTQCQRRKTAIKPLIMD
AHVVVGVGNIYASESLFHAGIRPGVAAHRLSRAACARLADAIKQVLTAAIAAGGSSLRDYVQSSGELGYFQLQTRVYDRE
DAPCRRCATPIRRIVQAQRASFYCPTCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=270, Percent_Identity=50.7407407407407, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1786932, Length=275, Percent_Identity=26.5454545454545, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_THIDA (Q3SLR9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314141.1
- ProteinModelPortal:   Q3SLR9
- SMR:   Q3SLR9
- STRING:   Q3SLR9
- GeneID:   3672770
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0383
- NMPDR:   fig|292415.3.peg.777
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- PhylomeDB:   Q3SLR9
- BioCyc:   TDEN292415:TBD_0383-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 29839; Mature: 29708

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 260-260 BINDING 91-91 BINDING 110-110 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETTRAGLSPRLQGRVLTRVIVREPRLRWPIPPDLDTQLRGLTLHGLARRGKYL
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCEEHHHHHHCCCEE
LFDFGAVTQLVHLGMSGSLRLTEPAEPAARHDHVDWRFDDGTILRLRDPRRFGAVLLTEN
EEEHHHHHHHHHHCCCCCEEECCCCCCCHHCCCCCEEECCCEEEEEECCCCCCEEEEECC
APGHPLLAGLGPEPLSTAFDAAYLHTQCQRRKTAIKPLIMDAHVVVGVGNIYASESLFHA
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHC
GIRPGVAAHRLSRAACARLADAIKQVLTAAIAAGGSSLRDYVQSSGELGYFQLQTRVYDR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCC
EDAPCRRCATPIRRIVQAQRASFYCPTCQR
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PELPEVETTRAGLSPRLQGRVLTRVIVREPRLRWPIPPDLDTQLRGLTLHGLARRGKYL
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCEEHHHHHHCCCEE
LFDFGAVTQLVHLGMSGSLRLTEPAEPAARHDHVDWRFDDGTILRLRDPRRFGAVLLTEN
EEEHHHHHHHHHHCCCCCEEECCCCCCCHHCCCCCEEECCCEEEEEECCCCCCEEEEECC
APGHPLLAGLGPEPLSTAFDAAYLHTQCQRRKTAIKPLIMDAHVVVGVGNIYASESLFHA
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHC
GIRPGVAAHRLSRAACARLADAIKQVLTAAIAAGGSSLRDYVQSSGELGYFQLQTRVYDR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCC
EDAPCRRCATPIRRIVQAQRASFYCPTCQR
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA