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The map label for this gene is 74316396

Identifier: 74316396

GI number: 74316396

Start: 406529

End: 407836

Strand: Direct

Name: 74316396

Synonym: Tbd_0378

Alternate gene names: NA

Gene position: 406529-407836 (Clockwise)

Preceding gene: 74316395

Following gene: 74316397

Centisome position: 13.97

GC content: 66.9

Gene sequence:

>1308_bases
ATGTCAATTAAGCGTTTCTTCGCCGTTCTCGTGCTGATCCTGGCGGCGCCGGTGCAGGCCGGGGTCGACATCCAGCATTG
GCAGACCCCCGAGGGCGCGCGGGTCTTCTTCGTCGAAAGCCATGAGCTGCCGATCCTCGATATCTCCGTCGACTTCCGCG
CCGGCAGCGCGCGTGACCCGGTCGACAAGAGCGGTCTCGCGCGGCTCACCCACGCCCTGCTCGACCAGGGCGCGGGCGGC
CTCAGCGACACCGAGATCGCCCATCGGCTGGCCGATGTCGGTGCCGTGCTGGGCGGCAATTTCGACCGCGATCGCGCGGG
CGTGACCTTGCGTACGCTCTCGTCGATGGCCGAAAAGACGGCCGCGCTGGACACACTCGTCCGCGTGCTGCAGCAGCCGG
ACTTTCCGCTTGCGGTGATGCAGCGTGAACGGCGGCGCCTCATTTCGTCGATTCGCGAAGCCGAGGCCGACCCCGGAACC
GTCGCCGAGAAGGCGTTCTACCGCGCGCTATACGCCGACCATCCCTATGCGCGCGACGAGAACGGCGTGCCGGGCAATCT
CGAGAAGCTGACCCGCAACGACGTGAGCGCGTTCTACCGCGCGCATTACGGCGCCCCAAACGCGGTCATCTCGCTGATCG
GCGCGATCAGCCGCAGCGAGGCCGAGGCGATCGCGACGCGCCTCGCCGAGGGTCTGCCGGCGGCAGCGTCGCTCGCCGAT
CTGCCGCTGCCGGTCGCAGCGACCGCGTCGACCGTGCGTTTTGCGCACCCGTCGACGCAAAGCCATGTCCTGATGGGCGC
GGTCGGCGTGGCGCGCAGCGACCCCGACTATTTCCCGCTTTTCGTGGGCAACTATGCCCTCGGCGGCGGCGGGTTCGACT
CGCGCCTGATGCGCGAGGTGCGCGACAAGCGCGGCTTCGCCTACAGCGCCTACAGTTACTTCCTGCCAATGCTGCAGGCC
GGGCCGTTCCAGCTGGGGCTGCAGACCAAGCTCGAGCAGACCGACGAAGCGATGGAGGTCGCACGCTCGACGCTGCAGCA
ATTCATCGCCGAGGGGCCGAGCGAGGCCGAACTCGTGCAGGCCAAGGCCAACCTCACCGGCGGTTTCCCGCTGCGCATCG
ACAGCAACAAGAAGATCCTCGAATACCTTGCCGTGATCGGTTTCTACAACCTGCCTCTGGATTATCTCGAGACCTGGACC
GACAAGGTCAATGCGGTCGACACTGCGGCGGTCAGACAGGCCTTCGCGCGCCATCTCGACCCGGAGCGTCTGGTTACCGT
CGTCGTCGGAGCCGAAGGTGCTCGCTAA

Upstream 100 bases:

>100_bases
GCGCAGGAGTGGTTGCGCGAAGACCGCCTGAGCGTGGCCGAACTCGACCCCCAGGCGCTCAAACCGCAGCCTGAACGCAC
GGCCGTGCCGGGAGTACGTC

Downstream 100 bases:

>100_bases
GCAGGCCGCGCCGAAGCGCGCGCACGGCGCCGCCAATCGCGTCCGCATCGTCGGCGGACGCTATCGCAGCCGGCTGCTCG
CGTTTCCCGACGGCACCGGC

Product: insulinase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSIKRFFAVLVLILAAPVQAGVDIQHWQTPEGARVFFVESHELPILDISVDFRAGSARDPVDKSGLARLTHALLDQGAGG
LSDTEIAHRLADVGAVLGGNFDRDRAGVTLRTLSSMAEKTAALDTLVRVLQQPDFPLAVMQRERRRLISSIREAEADPGT
VAEKAFYRALYADHPYARDENGVPGNLEKLTRNDVSAFYRAHYGAPNAVISLIGAISRSEAEAIATRLAEGLPAAASLAD
LPLPVAATASTVRFAHPSTQSHVLMGAVGVARSDPDYFPLFVGNYALGGGGFDSRLMREVRDKRGFAYSAYSYFLPMLQA
GPFQLGLQTKLEQTDEAMEVARSTLQQFIAEGPSEAELVQAKANLTGGFPLRIDSNKKILEYLAVIGFYNLPLDYLETWT
DKVNAVDTAAVRQAFARHLDPERLVTVVVGAEGAR

Sequences:

>Translated_435_residues
MSIKRFFAVLVLILAAPVQAGVDIQHWQTPEGARVFFVESHELPILDISVDFRAGSARDPVDKSGLARLTHALLDQGAGG
LSDTEIAHRLADVGAVLGGNFDRDRAGVTLRTLSSMAEKTAALDTLVRVLQQPDFPLAVMQRERRRLISSIREAEADPGT
VAEKAFYRALYADHPYARDENGVPGNLEKLTRNDVSAFYRAHYGAPNAVISLIGAISRSEAEAIATRLAEGLPAAASLAD
LPLPVAATASTVRFAHPSTQSHVLMGAVGVARSDPDYFPLFVGNYALGGGGFDSRLMREVRDKRGFAYSAYSYFLPMLQA
GPFQLGLQTKLEQTDEAMEVARSTLQQFIAEGPSEAELVQAKANLTGGFPLRIDSNKKILEYLAVIGFYNLPLDYLETWT
DKVNAVDTAAVRQAFARHLDPERLVTVVVGAEGAR
>Mature_434_residues
SIKRFFAVLVLILAAPVQAGVDIQHWQTPEGARVFFVESHELPILDISVDFRAGSARDPVDKSGLARLTHALLDQGAGGL
SDTEIAHRLADVGAVLGGNFDRDRAGVTLRTLSSMAEKTAALDTLVRVLQQPDFPLAVMQRERRRLISSIREAEADPGTV
AEKAFYRALYADHPYARDENGVPGNLEKLTRNDVSAFYRAHYGAPNAVISLIGAISRSEAEAIATRLAEGLPAAASLADL
PLPVAATASTVRFAHPSTQSHVLMGAVGVARSDPDYFPLFVGNYALGGGGFDSRLMREVRDKRGFAYSAYSYFLPMLQAG
PFQLGLQTKLEQTDEAMEVARSTLQQFIAEGPSEAELVQAKANLTGGFPLRIDSNKKILEYLAVIGFYNLPLDYLETWTD
KVNAVDTAAVRQAFARHLDPERLVTVVVGAEGAR

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Homo sapiens, GI94538354, Length=394, Percent_Identity=19.5431472081218, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323192, Length=346, Percent_Identity=23.9884393063584, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357875, Length=377, Percent_Identity=22.0159151193634, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646943, Length=377, Percent_Identity=22.0159151193634, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR007863 [H]

Pfam domain/function: PF05193 Peptidase_M16_C [H]

EC number: NA

Molecular weight: Translated: 47137; Mature: 47006

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKRFFAVLVLILAAPVQAGVDIQHWQTPEGARVFFVESHELPILDISVDFRAGSARDP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCC
VDKSGLARLTHALLDQGAGGLSDTEIAHRLADVGAVLGGNFDRDRAGVTLRTLSSMAEKT
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHH
AALDTLVRVLQQPDFPLAVMQRERRRLISSIREAEADPGTVAEKAFYRALYADHPYARDE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
NGVPGNLEKLTRNDVSAFYRAHYGAPNAVISLIGAISRSEAEAIATRLAEGLPAAASLAD
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
LPLPVAATASTVRFAHPSTQSHVLMGAVGVARSDPDYFPLFVGNYALGGGGFDSRLMREV
CCCCCHHCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCEECCCCCHHHHHHHHH
RDKRGFAYSAYSYFLPMLQAGPFQLGLQTKLEQTDEAMEVARSTLQQFIAEGPSEAELVQ
HHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AKANLTGGFPLRIDSNKKILEYLAVIGFYNLPLDYLETWTDKVNAVDTAAVRQAFARHLD
HHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PERLVTVVVGAEGAR
HHHEEEEEECCCCCC
>Mature Secondary Structure 
SIKRFFAVLVLILAAPVQAGVDIQHWQTPEGARVFFVESHELPILDISVDFRAGSARDP
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCC
VDKSGLARLTHALLDQGAGGLSDTEIAHRLADVGAVLGGNFDRDRAGVTLRTLSSMAEKT
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHH
AALDTLVRVLQQPDFPLAVMQRERRRLISSIREAEADPGTVAEKAFYRALYADHPYARDE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
NGVPGNLEKLTRNDVSAFYRAHYGAPNAVISLIGAISRSEAEAIATRLAEGLPAAASLAD
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
LPLPVAATASTVRFAHPSTQSHVLMGAVGVARSDPDYFPLFVGNYALGGGGFDSRLMREV
CCCCCHHCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCEECCCCCHHHHHHHHH
RDKRGFAYSAYSYFLPMLQAGPFQLGLQTKLEQTDEAMEVARSTLQQFIAEGPSEAELVQ
HHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AKANLTGGFPLRIDSNKKILEYLAVIGFYNLPLDYLETWTDKVNAVDTAAVRQAFARHLD
HHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PERLVTVVVGAEGAR
HHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]