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The map label for this gene is rsmD [H]

Identifier: 74316397

GI number: 74316397

Start: 407826

End: 408398

Strand: Direct

Name: rsmD [H]

Synonym: Tbd_0379

Alternate gene names: 74316397

Gene position: 407826-408398 (Clockwise)

Preceding gene: 74316396

Following gene: 74316398

Centisome position: 14.02

GC content: 71.2

Gene sequence:

>573_bases
GTGCTCGCTAAGCAGGCCGCGCCGAAGCGCGCGCACGGCGCCGCCAATCGCGTCCGCATCGTCGGCGGACGCTATCGCAG
CCGGCTGCTCGCGTTTCCCGACGGCACCGGCTTGCGACCAACGCCCGACCGCGTGCGCGAAACCCTGTTCAACTGGCTGG
GACAGGATCTGGCGGGCTGGCGCTGCGTCGACCTGTTCGCCGGCAGCGGCGCGCTCGGTTTCGAAGCCGCTTCGCGCGGC
GCCGCGCGTGTGCTGATGATCGAGCGCGACGCGAACGCGGTCGCCGCGTTGGAGAAGAACCGCGGCGCGCTCGGCGCCGC
GGTCGACATCGTCCGCGCCGACGCGCTCGCCTGGCTCGGTGGCAACCGCGAAACCTTCGATCTCGCCTTCGTCGATCCGC
CCTTCGACAGCGACATGGCGGCGTTGGCGCTCGCCGGCCTTGCGCCGCATATCGCATCGGGCGGATACGTCTACGTCGAG
CAGGGGGCGGAGACGGCCGTTCCGCCGGGGTTCGTCGTCCACCGCAGCGGGCGCGCCGGACGTTCGCATTTCGCGCTGCT
CATGAAGGAATAG

Upstream 100 bases:

>100_bases
ACCGACAAGGTCAATGCGGTCGACACTGCGGCGGTCAGACAGGCCTTCGCGCGCCATCTCGACCCGGAGCGTCTGGTTAC
CGTCGTCGTCGGAGCCGAAG

Downstream 100 bases:

>100_bases
CGATGCTGACCGCCGTCTATCCAGGCACCTTCGACCCCATCACGCGCGGCCACGAGGACCTCGTGCGACGCGCCGTCCGC
CTCTTCGACCGCGTCGTCGT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLAKQAAPKRAHGAANRVRIVGGRYRSRLLAFPDGTGLRPTPDRVRETLFNWLGQDLAGWRCVDLFAGSGALGFEAASRG
AARVLMIERDANAVAALEKNRGALGAAVDIVRADALAWLGGNRETFDLAFVDPPFDSDMAALALAGLAPHIASGGYVYVE
QGAETAVPPGFVVHRSGRAGRSHFALLMKE

Sequences:

>Translated_190_residues
MLAKQAAPKRAHGAANRVRIVGGRYRSRLLAFPDGTGLRPTPDRVRETLFNWLGQDLAGWRCVDLFAGSGALGFEAASRG
AARVLMIERDANAVAALEKNRGALGAAVDIVRADALAWLGGNRETFDLAFVDPPFDSDMAALALAGLAPHIASGGYVYVE
QGAETAVPPGFVVHRSGRAGRSHFALLMKE
>Mature_190_residues
MLAKQAAPKRAHGAANRVRIVGGRYRSRLLAFPDGTGLRPTPDRVRETLFNWLGQDLAGWRCVDLFAGSGALGFEAASRG
AARVLMIERDANAVAALEKNRGALGAAVDIVRADALAWLGGNRETFDLAFVDPPFDSDMAALALAGLAPHIASGGYVYVE
QGAETAVPPGFVVHRSGRAGRSHFALLMKE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=188, Percent_Identity=39.3617021276596, Blast_Score=127, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 20137; Mature: 20137

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKQAAPKRAHGAANRVRIVGGRYRSRLLAFPDGTGLRPTPDRVRETLFNWLGQDLAGW
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCC
RCVDLFAGSGALGFEAASRGAARVLMIERDANAVAALEKNRGALGAAVDIVRADALAWLG
EEEEEECCCCCCCCHHCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHEEEC
GNRETFDLAFVDPPFDSDMAALALAGLAPHIASGGYVYVEQGAETAVPPGFVVHRSGRAG
CCCCEEEEEEECCCCCCHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCCEEEECCCCCC
RSHFALLMKE
CCEEEEEEEC
>Mature Secondary Structure
MLAKQAAPKRAHGAANRVRIVGGRYRSRLLAFPDGTGLRPTPDRVRETLFNWLGQDLAGW
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCC
RCVDLFAGSGALGFEAASRGAARVLMIERDANAVAALEKNRGALGAAVDIVRADALAWLG
EEEEEECCCCCCCCHHCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHEEEC
GNRETFDLAFVDPPFDSDMAALALAGLAPHIASGGYVYVEQGAETAVPPGFVVHRSGRAG
CCCCEEEEEEECCCCCCHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCCEEEECCCCCC
RSHFALLMKE
CCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]