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The map label for this gene is ppdK [H]

Identifier: 74316386

GI number: 74316386

Start: 398164

End: 398883

Strand: Direct

Name: ppdK [H]

Synonym: Tbd_0368

Alternate gene names: 74316386

Gene position: 398164-398883 (Clockwise)

Preceding gene: 74316385

Following gene: 74316387

Centisome position: 13.68

GC content: 63.89

Gene sequence:

>720_bases
ATGCCTAAAGGAAAAAGCAAGTCCGCCGCACGGCTGCCCGTGTTCGACCTGAACGGTCGAGACGGCGTGCCGGACGTGCG
CATCGTCGGCTTCAAGGCACACAACCTGATCCGCATGGCCGCGATGGGTTTGCCGGTGCCGCAGGGCTTCGTCATGGGCA
CTGACTGGTGCGGGGCCATCCGGAACGGCGAGGGTGTGAAGGTGCATTTGCCCGATGCGCTGCAATCCGGCATCCGCGAC
CTGGAACGAGCCACCGGCCTTGGGTTCGGCAGCGCGCGCCGCCCTCTGCTGGTTTCGGTGCGCTCGGGCGCACCGGTTTC
CATGCCGGGCATGATGGAAACGGTGCTTAATGTCGGACTGAATGATGTGTCGCTGGCAGGGCTGTTGCGGCTGACCGGCG
ATCACCGTCTGGTGTGGGATTCCTATCGCCGCCTGATCCAGGCCTTTGCCGAGATCGTAGCCGTTATCCCGGCCGAGCCG
TTCGAGGCTGCGCTGGATGCCACCCTGCGCCGGGCCGGCGCAGACGTTGCGCGCCAGCTCGACTTCCAGGCCCTGCGCGA
CCTGGCGCACATCTACCTCGACCTCTACCACGGCCACACCGGCCGGGAATTCCCCCAGGATCCATTCGAGCAGCTGTGGA
TGGCGTGCAGGCGATTGAAAGCTCTGCTGGGAAAAGTGATTTCGAACTGCATCAAGTTCTGGAAGGCGCTCGAAACATAA

Upstream 100 bases:

>100_bases
TTCGGCATCGTCGAGGCAGCGCGCAATCTCGAGGAGGCAGCCGACGGCCTCATGCACGTGGCCCTGCTGCTGCGCGATCA
TGTGATGGGCAAGGTGATTC

Downstream 100 bases:

>100_bases
TCAACGGCAACGCCATATTTCTACACGACATCTATCAAAGCAAGGTATGGAATGAGGTCTATGGATCGAAAGCCTAACCC
GGCGGTCAAGGCGCTCCCTC

Product: pyruvate,orthophosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MPKGKSKSAARLPVFDLNGRDGVPDVRIVGFKAHNLIRMAAMGLPVPQGFVMGTDWCGAIRNGEGVKVHLPDALQSGIRD
LERATGLGFGSARRPLLVSVRSGAPVSMPGMMETVLNVGLNDVSLAGLLRLTGDHRLVWDSYRRLIQAFAEIVAVIPAEP
FEAALDATLRRAGADVARQLDFQALRDLAHIYLDLYHGHTGREFPQDPFEQLWMACRRLKALLGKVISNCIKFWKALET

Sequences:

>Translated_239_residues
MPKGKSKSAARLPVFDLNGRDGVPDVRIVGFKAHNLIRMAAMGLPVPQGFVMGTDWCGAIRNGEGVKVHLPDALQSGIRD
LERATGLGFGSARRPLLVSVRSGAPVSMPGMMETVLNVGLNDVSLAGLLRLTGDHRLVWDSYRRLIQAFAEIVAVIPAEP
FEAALDATLRRAGADVARQLDFQALRDLAHIYLDLYHGHTGREFPQDPFEQLWMACRRLKALLGKVISNCIKFWKALET
>Mature_238_residues
PKGKSKSAARLPVFDLNGRDGVPDVRIVGFKAHNLIRMAAMGLPVPQGFVMGTDWCGAIRNGEGVKVHLPDALQSGIRDL
ERATGLGFGSARRPLLVSVRSGAPVSMPGMMETVLNVGLNDVSLAGLLRLTGDHRLVWDSYRRLIQAFAEIVAVIPAEPF
EAALDATLRRAGADVARQLDFQALRDLAHIYLDLYHGHTGREFPQDPFEQLWMACRRLKALLGKVISNCIKFWKALET

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 26221; Mature: 26090

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGKSKSAARLPVFDLNGRDGVPDVRIVGFKAHNLIRMAAMGLPVPQGFVMGTDWCGAI
CCCCCCCCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCEEECCCHHHHC
RNGEGVKVHLPDALQSGIRDLERATGLGFGSARRPLLVSVRSGAPVSMPGMMETVLNVGL
CCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCC
NDVSLAGLLRLTGDHRLVWDSYRRLIQAFAEIVAVIPAEPFEAALDATLRRAGADVARQL
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DFQALRDLAHIYLDLYHGHTGREFPQDPFEQLWMACRRLKALLGKVISNCIKFWKALET
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKGKSKSAARLPVFDLNGRDGVPDVRIVGFKAHNLIRMAAMGLPVPQGFVMGTDWCGAI
CCCCCCCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCEEECCCHHHHC
RNGEGVKVHLPDALQSGIRDLERATGLGFGSARRPLLVSVRSGAPVSMPGMMETVLNVGL
CCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCC
NDVSLAGLLRLTGDHRLVWDSYRRLIQAFAEIVAVIPAEPFEAALDATLRRAGADVARQL
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DFQALRDLAHIYLDLYHGHTGREFPQDPFEQLWMACRRLKALLGKVISNCIKFWKALET
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]