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The map label for this gene is mrdA [H]

Identifier: 74316281

GI number: 74316281

Start: 283766

End: 285658

Strand: Direct

Name: mrdA [H]

Synonym: Tbd_0263

Alternate gene names: 74316281

Gene position: 283766-285658 (Clockwise)

Preceding gene: 74316280

Following gene: 74316282

Centisome position: 9.75

GC content: 68.25

Gene sequence:

>1893_bases
ATGAGAATCGTCACCCCGCTGAAGAATCTCGACCGCGAGCTTGCCCGTTTCCGCGGGCGCGTGAAGTTCGGCGCGGCCTT
CGCGCTGCTGCTGGCCGGCGTGCTGCTCGCGCGCGCCTTCCACCTCCAGGTCGTCGCGCATGAGCACTACATCGAGCGTG
CCGAAAGCAACCGCATTTCGCTGGTGCCGGCGCCGCCGAACCGCGGCCTGATACTCGACCGCAAGGGCCGCGTGCTCGCC
GAAAACTATTCGGCGTACACGGTCGAGCTGACGCGCGCGGAGGCCGACGACATGGAGGCCACGCTCGCCGAGATCGGCAA
GCTGATCGAGATCACCCCGGGCCAGCTTCGCCGCTTCCGGCGTCTGCTCGCCGAATCGCACGAGTTCGAGACCGTCCCGC
TCAAGAGCAAGCTGACCGACGAGGAGGTCGCGATTCTTGCCGCCAACCTTTATCGCCTGCCCGGCGCGGAGGTGCGCGCC
CGCCTGTTCCGCAATTACCAGGGCGGGCCGGGCATGGCGCACGTCGTCGGGTTCATCGGCCGCATCAACGACCGCGACCT
CAGGCGTGTGCGCGAGTCGGGGCTCGACAAGGACTACAAGGGCACGGTCCATATCGGGAAAACGGGACTCGAGCAGAGCT
ACGAGACGCTGTTGCACGGCCGCGCGGGTTTCGACCGCATGGAGACCGACGCGAGCGGCCGCGCCGTGCGCATGATCTCG
CGCATCCCGCCGGTGCCGGGAAAAGATCTGCGTCTGCATCTCGACGCGTCGCTGCAGGAAGTCGCCGAGCACGCGTTCGG
CGACTATCTCGGCGGCCTGGTCGCGCTCGACCCCAACACCGGGGGCGTCCTCGCGCTCGTGAGCAAGCCGGGTTTCGATC
CCAATCTGTTCGTCGACGGTATCGATCCGGAAACCTGGAAGTCGCTCAACGAGTCGCCCGAACGGCCGATGGTCAATCGC
GCCCTGCGCGGCATCTATCCGCCCGGCTCGACGATCAAGCCGCTGATGGCCCTGGCCGGCCTCGAACTCGGCCTGCGCGA
GCCCGACGACAGCATCGACGACCCGGGGTTTTTCCGCCTACCCAACAGCCAGCACCGTTTCCGCGACTGGAAGCCCGGTG
GTCACGGCATCGTCGATCTGCGCCGATCGATTTCGCAGTCGTGCGACATCTACTATTACCGTCTCGCGGTCGACATGGGC
ATCGACCGCATGCACGACTACCTTACGCAATTCGGCCTCGGCCAGAAGACCGGCATCGACCTGCAGGGCGAGTCGTCGGG
GCTGTTGCCGTCGCGCGCGTGGAAGCAGCGGCGCTGGAAGCAGCCCTGGTACCCGGGCGAGACCGTGATCGCCGGCATCG
GCCAGGGCTATCACCTGACCACGCCGCTGCAGCTCGCGACGATGACGGCCATGCTTGCCAACGGCGGCAAGCGCATCCAG
CCCCGGCTGGTCGACGCCGTACGCGACCCGCATACGCGGGCCTGGCAGACGCAGGCGCCGGTCATGCTCGGTGAGGTGGC
GATCGACCCGGAGAATCTCGCGGTCGTGCGCCAGGGCATGATGGACGTCACCCGCCCGGGTGGTACCGCGGCGGCGTCGG
CGGCCGGCGCCGCGTATGGCATCGCCGGCAAGACCGGCACCGCGCAGGTCGTCGGCATCAAGCAGGGGGCGCGCTACGAC
GCGACGCGGCTCGCCCGCGAGCACCGCGACCACGCACTCTTCGTCGCCTACGCGCCGGCCGAGAACCCCGTTATCGCGGT
CGCCGTGATGGTCGAGAACGGCGGTTCGGGCAGCGGCACCGCAGCGCCGATCGCGCGCGCCGTGTTCGACTACTACCTGA
CCGGGAAACGACCCGGCACCATGAAGCTGGAGACCGACGATGCAGCGGACTAG

Upstream 100 bases:

>100_bases
CTGAAGCCGGAGTCACGCTGATGCCTCGCCGTATCGCCCGGGCTCCGCTGCACTTCGACGCTTGCGTGCGGTGTCGCGCC
CGCCCCTCGCGGGAACGGGC

Downstream 100 bases:

>100_bases
GCACTGGATCCTGCGCCGGCTGAGCCACCTCGACCTCATTCTGCTCGCGCTCGTGCTGCTGCTGCTCGGGATCAGCGTCG
CGGTCGTCGCGAGCGCGTCG

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MRIVTPLKNLDRELARFRGRVKFGAAFALLLAGVLLARAFHLQVVAHEHYIERAESNRISLVPAPPNRGLILDRKGRVLA
ENYSAYTVELTRAEADDMEATLAEIGKLIEITPGQLRRFRRLLAESHEFETVPLKSKLTDEEVAILAANLYRLPGAEVRA
RLFRNYQGGPGMAHVVGFIGRINDRDLRRVRESGLDKDYKGTVHIGKTGLEQSYETLLHGRAGFDRMETDASGRAVRMIS
RIPPVPGKDLRLHLDASLQEVAEHAFGDYLGGLVALDPNTGGVLALVSKPGFDPNLFVDGIDPETWKSLNESPERPMVNR
ALRGIYPPGSTIKPLMALAGLELGLREPDDSIDDPGFFRLPNSQHRFRDWKPGGHGIVDLRRSISQSCDIYYYRLAVDMG
IDRMHDYLTQFGLGQKTGIDLQGESSGLLPSRAWKQRRWKQPWYPGETVIAGIGQGYHLTTPLQLATMTAMLANGGKRIQ
PRLVDAVRDPHTRAWQTQAPVMLGEVAIDPENLAVVRQGMMDVTRPGGTAAASAAGAAYGIAGKTGTAQVVGIKQGARYD
ATRLAREHRDHALFVAYAPAENPVIAVAVMVENGGSGSGTAAPIARAVFDYYLTGKRPGTMKLETDDAAD

Sequences:

>Translated_630_residues
MRIVTPLKNLDRELARFRGRVKFGAAFALLLAGVLLARAFHLQVVAHEHYIERAESNRISLVPAPPNRGLILDRKGRVLA
ENYSAYTVELTRAEADDMEATLAEIGKLIEITPGQLRRFRRLLAESHEFETVPLKSKLTDEEVAILAANLYRLPGAEVRA
RLFRNYQGGPGMAHVVGFIGRINDRDLRRVRESGLDKDYKGTVHIGKTGLEQSYETLLHGRAGFDRMETDASGRAVRMIS
RIPPVPGKDLRLHLDASLQEVAEHAFGDYLGGLVALDPNTGGVLALVSKPGFDPNLFVDGIDPETWKSLNESPERPMVNR
ALRGIYPPGSTIKPLMALAGLELGLREPDDSIDDPGFFRLPNSQHRFRDWKPGGHGIVDLRRSISQSCDIYYYRLAVDMG
IDRMHDYLTQFGLGQKTGIDLQGESSGLLPSRAWKQRRWKQPWYPGETVIAGIGQGYHLTTPLQLATMTAMLANGGKRIQ
PRLVDAVRDPHTRAWQTQAPVMLGEVAIDPENLAVVRQGMMDVTRPGGTAAASAAGAAYGIAGKTGTAQVVGIKQGARYD
ATRLAREHRDHALFVAYAPAENPVIAVAVMVENGGSGSGTAAPIARAVFDYYLTGKRPGTMKLETDDAAD
>Mature_630_residues
MRIVTPLKNLDRELARFRGRVKFGAAFALLLAGVLLARAFHLQVVAHEHYIERAESNRISLVPAPPNRGLILDRKGRVLA
ENYSAYTVELTRAEADDMEATLAEIGKLIEITPGQLRRFRRLLAESHEFETVPLKSKLTDEEVAILAANLYRLPGAEVRA
RLFRNYQGGPGMAHVVGFIGRINDRDLRRVRESGLDKDYKGTVHIGKTGLEQSYETLLHGRAGFDRMETDASGRAVRMIS
RIPPVPGKDLRLHLDASLQEVAEHAFGDYLGGLVALDPNTGGVLALVSKPGFDPNLFVDGIDPETWKSLNESPERPMVNR
ALRGIYPPGSTIKPLMALAGLELGLREPDDSIDDPGFFRLPNSQHRFRDWKPGGHGIVDLRRSISQSCDIYYYRLAVDMG
IDRMHDYLTQFGLGQKTGIDLQGESSGLLPSRAWKQRRWKQPWYPGETVIAGIGQGYHLTTPLQLATMTAMLANGGKRIQ
PRLVDAVRDPHTRAWQTQAPVMLGEVAIDPENLAVVRQGMMDVTRPGGTAAASAAGAAYGIAGKTGTAQVVGIKQGARYD
ATRLAREHRDHALFVAYAPAENPVIAVAVMVENGGSGSGTAAPIARAVFDYYLTGKRPGTMKLETDDAAD

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=617, Percent_Identity=40.5186385737439, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1786272, Length=637, Percent_Identity=25.4317111459969, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69053; Mature: 69053

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVTPLKNLDRELARFRGRVKFGAAFALLLAGVLLARAFHLQVVAHEHYIERAESNRIS
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVPAPPNRGLILDRKGRVLAENYSAYTVELTRAEADDMEATLAEIGKLIEITPGQLRRFR
EEECCCCCCEEEECCCCEEEECCCEEEEEEECCCCCHHHHHHHHHCCEEECCCHHHHHHH
RLLAESHEFETVPLKSKLTDEEVAILAANLYRLPGAEVRARLFRNYQGGPGMAHVVGFIG
HHHHCCCCCEECCCHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHH
RINDRDLRRVRESGLDKDYKGTVHIGKTGLEQSYETLLHGRAGFDRMETDASGRAVRMIS
CCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHH
RIPPVPGKDLRLHLDASLQEVAEHAFGDYLGGLVALDPNTGGVLALVSKPGFDPNLFVDG
HCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCEEEEC
IDPETWKSLNESPERPMVNRALRGIYPPGSTIKPLMALAGLELGLREPDDSIDDPGFFRL
CCHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
PNSQHRFRDWKPGGHGIVDLRRSISQSCDIYYYRLAVDMGIDRMHDYLTQFGLGQKTGID
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEHHCHHHHHHHHHHHCCCCCCCCC
LQGESSGLLPSRAWKQRRWKQPWYPGETVIAGIGQGYHLTTPLQLATMTAMLANGGKRIQ
CCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHCCCCCCC
PRLVDAVRDPHTRAWQTQAPVMLGEVAIDPENLAVVRQGMMDVTRPGGTAAASAAGAAYG
HHHHHHHCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEE
IAGKTGTAQVVGIKQGARYDATRLAREHRDHALFVAYAPAENPVIAVAVMVENGGSGSGT
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCC
AAPIARAVFDYYLTGKRPGTMKLETDDAAD
HHHHHHHHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MRIVTPLKNLDRELARFRGRVKFGAAFALLLAGVLLARAFHLQVVAHEHYIERAESNRIS
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVPAPPNRGLILDRKGRVLAENYSAYTVELTRAEADDMEATLAEIGKLIEITPGQLRRFR
EEECCCCCCEEEECCCCEEEECCCEEEEEEECCCCCHHHHHHHHHCCEEECCCHHHHHHH
RLLAESHEFETVPLKSKLTDEEVAILAANLYRLPGAEVRARLFRNYQGGPGMAHVVGFIG
HHHHCCCCCEECCCHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHH
RINDRDLRRVRESGLDKDYKGTVHIGKTGLEQSYETLLHGRAGFDRMETDASGRAVRMIS
CCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHH
RIPPVPGKDLRLHLDASLQEVAEHAFGDYLGGLVALDPNTGGVLALVSKPGFDPNLFVDG
HCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCEEEEC
IDPETWKSLNESPERPMVNRALRGIYPPGSTIKPLMALAGLELGLREPDDSIDDPGFFRL
CCHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
PNSQHRFRDWKPGGHGIVDLRRSISQSCDIYYYRLAVDMGIDRMHDYLTQFGLGQKTGID
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEHHCHHHHHHHHHHHCCCCCCCCC
LQGESSGLLPSRAWKQRRWKQPWYPGETVIAGIGQGYHLTTPLQLATMTAMLANGGKRIQ
CCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHCCCCCCC
PRLVDAVRDPHTRAWQTQAPVMLGEVAIDPENLAVVRQGMMDVTRPGGTAAASAAGAAYG
HHHHHHHCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEE
IAGKTGTAQVVGIKQGARYDATRLAREHRDHALFVAYAPAENPVIAVAVMVENGGSGSGT
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCC
AAPIARAVFDYYLTGKRPGTMKLETDDAAD
HHHHHHHHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]