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The map label for this gene is mrdB [H]

Identifier: 74316282

GI number: 74316282

Start: 285645

End: 286745

Strand: Direct

Name: mrdB [H]

Synonym: Tbd_0264

Alternate gene names: 74316282

Gene position: 285645-286745 (Clockwise)

Preceding gene: 74316281

Following gene: 74316283

Centisome position: 9.82

GC content: 65.4

Gene sequence:

>1101_bases
ATGCAGCGGACTAGGCACTGGATCCTGCGCCGGCTGAGCCACCTCGACCTCATTCTGCTCGCGCTCGTGCTGCTGCTGCT
CGGGATCAGCGTCGCGGTCGTCGCGAGCGCGTCGGGGCAGAGCCCGGCGCGCATCAGCGGTCACCTCGTCAACGTCGGGC
TCGCGCTGTCGCTCATGATCGTCATGGCCAACATCCCGCCGCACCTCCTGTCCAAGGTCGGGCCGGTCCTCTATGTCGCC
GGCGTCGTGCTGCTCGTTGCAGTCGCCGTGTTCGGCGAAATACGCAACGGTTCGCGGCGCTGGCTCAATCTCGGCGTCAT
GGCGTTCCAGCCGTCCGAACTGCTGAAACTCGCGGTGCCGCTCATGCTCGCCTGGTATTTCCAGCGCAACGAGTCAGGGC
TGCGCGGCAAGCATTTTCTCATCGGCGGTGCGCTGCTTGCCGTGCCTTTCGTGCTGATCCTGCGCCAGCCCGACCTTGGC
ACGGCGCTGATGATCGGCGCATCGGGCTTCTATCTGCTGTTCTTCGCCGGACTTCCGTGGAAGATCATCCTCGGCGGCGG
CGTGCTCGCCGGCGCGAGCCTCCCAGTCCTCTGGGGGTTTTTGCACGACTACCAGCGGGAACGCGTCCTCACACTGCTCG
ACCCTGCGTCGGACCCGCTCGGCGCCGGCTATCACATCATCCAGTCGACGATCGCGATCGGCTCGGGCGGCCTTTTCGGC
AAAGGCTGGAGCCAGGGCACGCAAAGCCAGCTCGACTTCATCCCCGAACGTACGACCGATTTCGTCTTCGCCGTATTCGG
TGAGGAGTTCGGCTATGTCGGCACGATCCTCCTCGTCGCGCTCTACCTTTCCATCGTCGCGCGCGGGCTCGTCATCGCGG
CGCGAGCGCCGACGCTCTTCGGCCGGCTCATGGCCGCCACCCTGAGCCTCAATCTGTTCACCTACGTGTTCGTCAACATG
GGCATGGTCTCGGGCATCCTGCCCGTGGTCGGCGTGCCGCTGCCACTGATGAGCTACGGCGGCACCGCGCTCGTCACGCT
GCTCCTGGGCATGGGCATCCTGATGAGCGTGGCGACCCATCGCCAGCTCAATAAAACCTGA

Upstream 100 bases:

>100_bases
GGCGGTTCGGGCAGCGGCACCGCAGCGCCGATCGCGCGCGCCGTGTTCGACTACTACCTGACCGGGAAACGACCCGGCAC
CATGAAGCTGGAGACCGACG

Downstream 100 bases:

>100_bases
CGGGATAAAATACCGCCATGAAAATCCTCCTGATGTCGGGCGCCGCCGCATTGGCGCTCACGCTCGCAGGCTGCGGCACC
ACGCCGCCCGCCAGGCAGAC

Product: rod shape-determining protein RodA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQRTRHWILRRLSHLDLILLALVLLLLGISVAVVASASGQSPARISGHLVNVGLALSLMIVMANIPPHLLSKVGPVLYVA
GVVLLVAVAVFGEIRNGSRRWLNLGVMAFQPSELLKLAVPLMLAWYFQRNESGLRGKHFLIGGALLAVPFVLILRQPDLG
TALMIGASGFYLLFFAGLPWKIILGGGVLAGASLPVLWGFLHDYQRERVLTLLDPASDPLGAGYHIIQSTIAIGSGGLFG
KGWSQGTQSQLDFIPERTTDFVFAVFGEEFGYVGTILLVALYLSIVARGLVIAARAPTLFGRLMAATLSLNLFTYVFVNM
GMVSGILPVVGVPLPLMSYGGTALVTLLLGMGILMSVATHRQLNKT

Sequences:

>Translated_366_residues
MQRTRHWILRRLSHLDLILLALVLLLLGISVAVVASASGQSPARISGHLVNVGLALSLMIVMANIPPHLLSKVGPVLYVA
GVVLLVAVAVFGEIRNGSRRWLNLGVMAFQPSELLKLAVPLMLAWYFQRNESGLRGKHFLIGGALLAVPFVLILRQPDLG
TALMIGASGFYLLFFAGLPWKIILGGGVLAGASLPVLWGFLHDYQRERVLTLLDPASDPLGAGYHIIQSTIAIGSGGLFG
KGWSQGTQSQLDFIPERTTDFVFAVFGEEFGYVGTILLVALYLSIVARGLVIAARAPTLFGRLMAATLSLNLFTYVFVNM
GMVSGILPVVGVPLPLMSYGGTALVTLLLGMGILMSVATHRQLNKT
>Mature_366_residues
MQRTRHWILRRLSHLDLILLALVLLLLGISVAVVASASGQSPARISGHLVNVGLALSLMIVMANIPPHLLSKVGPVLYVA
GVVLLVAVAVFGEIRNGSRRWLNLGVMAFQPSELLKLAVPLMLAWYFQRNESGLRGKHFLIGGALLAVPFVLILRQPDLG
TALMIGASGFYLLFFAGLPWKIILGGGVLAGASLPVLWGFLHDYQRERVLTLLDPASDPLGAGYHIIQSTIAIGSGGLFG
KGWSQGTQSQLDFIPERTTDFVFAVFGEEFGYVGTILLVALYLSIVARGLVIAARAPTLFGRLMAATLSLNLFTYVFVNM
GMVSGILPVVGVPLPLMSYGGTALVTLLLGMGILMSVATHRQLNKT

Specific function: This is one of the proteins responsible for the rod shape of E.coli. Is required for the expression of the enzymatic activity of PBP2, which is thought to synthesize peptidoglycan in the step of initiation of cell elongation [H]

COG id: COG0772

COG function: function code D; Bacterial cell division membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ftsW/rodA/spoVE family [H]

Homologues:

Organism=Escherichia coli, GI1786853, Length=351, Percent_Identity=55.2706552706553, Blast_Score=347, Evalue=8e-97,
Organism=Escherichia coli, GI1786277, Length=364, Percent_Identity=32.1428571428571, Blast_Score=158, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001182
- InterPro:   IPR018365
- InterPro:   IPR011923 [H]

Pfam domain/function: PF01098 FTSW_RODA_SPOVE [H]

EC number: NA

Molecular weight: Translated: 39203; Mature: 39203

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS00428 FTSW_RODA_SPOVE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTRHWILRRLSHLDLILLALVLLLLGISVAVVASASGQSPARISGHLVNVGLALSLMI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
VMANIPPHLLSKVGPVLYVAGVVLLVAVAVFGEIRNGSRRWLNLGVMAFQPSELLKLAVP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEEEECHHHHHHHHHH
LMLAWYFQRNESGLRGKHFLIGGALLAVPFVLILRQPDLGTALMIGASGFYLLFFAGLPW
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCE
KIILGGGVLAGASLPVLWGFLHDYQRERVLTLLDPASDPLGAGYHIIQSTIAIGSGGLFG
EEEECCCHHCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCC
KGWSQGTQSQLDFIPERTTDFVFAVFGEEFGYVGTILLVALYLSIVARGLVIAARAPTLF
CCCCCCCHHHHHHCCCCCHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHH
GRLMAATLSLNLFTYVFVNMGMVSGILPVVGVPLPLMSYGGTALVTLLLGMGILMSVATH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
RQLNKT
HHHCCC
>Mature Secondary Structure
MQRTRHWILRRLSHLDLILLALVLLLLGISVAVVASASGQSPARISGHLVNVGLALSLMI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
VMANIPPHLLSKVGPVLYVAGVVLLVAVAVFGEIRNGSRRWLNLGVMAFQPSELLKLAVP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEEEECHHHHHHHHHH
LMLAWYFQRNESGLRGKHFLIGGALLAVPFVLILRQPDLGTALMIGASGFYLLFFAGLPW
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCE
KIILGGGVLAGASLPVLWGFLHDYQRERVLTLLDPASDPLGAGYHIIQSTIAIGSGGLFG
EEEECCCHHCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCC
KGWSQGTQSQLDFIPERTTDFVFAVFGEEFGYVGTILLVALYLSIVARGLVIAARAPTLF
CCCCCCCHHHHHHCCCCCHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHH
GRLMAATLSLNLFTYVFVNMGMVSGILPVVGVPLPLMSYGGTALVTLLLGMGILMSVATH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
RQLNKT
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]