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The map label for this gene is nudF [H]

Identifier: 74316250

GI number: 74316250

Start: 254089

End: 254646

Strand: Direct

Name: nudF [H]

Synonym: Tbd_0232

Alternate gene names: 74316250

Gene position: 254089-254646 (Clockwise)

Preceding gene: 74316249

Following gene: 74316251

Centisome position: 8.73

GC content: 62.37

Gene sequence:

>558_bases
ATGGAAAACGACAGCCCCGACCTCGTCGAGCGCGAACTCGACAGCGAAACGGTATTCAAGGGGCGCTTGATGCACGTCAA
GCGCGACCGTGTGCAGCTCCCCGACGGCAAGGAATCGACGCGCGAATACATCGTCCACCCCGGTGCCGTCGTCGTGATCC
CCGTGTTCGACAATGGCGACCTCCTGCTCGAACGCCAATTTCGTTACCCGCTCCGCCGCGACTTCATCGAGCTGCCCGCC
GGCAAGATCGACCCGGGCGAGGACGACCTCACCTGCGCCAAACGCGAACTCGAAGAGGAAACCGGTTACACCGCGTCCGA
GTGGCGCGAGGTTACGACCATCTACCCGTGCATCGGTTATTCGGATGAGCGCCTTGCGTTCTACCTGGCGGAAGGGCTGC
GGGACGGCACCCACGGCCGTGACCACGACGAATTCCTCGAAGTGTTCCGCCTGCCTTTCGCCGAAGCGATGCAGTGGGTG
AGGGACGGTCGCATCTGCGAGACCAAGACCGTGATCGGGTTGTTCTGGCTGGAGAAGATGCTGGAGCAGGGATGGTAA

Upstream 100 bases:

>100_bases
CAGGCAGGAAGTTCTCCGCAATGAGGGACGTGTGTCCTCACTCTAGCCGGGTTCGCAAGAACCCGGATTTTTTTTGCCCG
CTCTGGGTATCATAAGCTGC

Downstream 100 bases:

>100_bases
AACACGTTCTAGTTGGTTTCCCGTTTCTTTCTTGGCGGTCTTCTCTCTATGACTAAATATCTTCTAGCAGTGCCGTTGCT
GTGGGCATGCAGTGCGCTGG

Product: hypothetical protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MENDSPDLVERELDSETVFKGRLMHVKRDRVQLPDGKESTREYIVHPGAVVVIPVFDNGDLLLERQFRYPLRRDFIELPA
GKIDPGEDDLTCAKRELEEETGYTASEWREVTTIYPCIGYSDERLAFYLAEGLRDGTHGRDHDEFLEVFRLPFAEAMQWV
RDGRICETKTVIGLFWLEKMLEQGW

Sequences:

>Translated_185_residues
MENDSPDLVERELDSETVFKGRLMHVKRDRVQLPDGKESTREYIVHPGAVVVIPVFDNGDLLLERQFRYPLRRDFIELPA
GKIDPGEDDLTCAKRELEEETGYTASEWREVTTIYPCIGYSDERLAFYLAEGLRDGTHGRDHDEFLEVFRLPFAEAMQWV
RDGRICETKTVIGLFWLEKMLEQGW
>Mature_185_residues
MENDSPDLVERELDSETVFKGRLMHVKRDRVQLPDGKESTREYIVHPGAVVVIPVFDNGDLLLERQFRYPLRRDFIELPA
GKIDPGEDDLTCAKRELEEETGYTASEWREVTTIYPCIGYSDERLAFYLAEGLRDGTHGRDHDEFLEVFRLPFAEAMQWV
RDGRICETKTVIGLFWLEKMLEQGW

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788810, Length=140, Percent_Identity=32.1428571428571, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789800, Length=172, Percent_Identity=26.7441860465116, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 21586; Mature: 21586

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDSPDLVERELDSETVFKGRLMHVKRDRVQLPDGKESTREYIVHPGAVVVIPVFDNGD
CCCCCHHHHHHHCCCHHHHHHHHHEEHHCCCCCCCCCCHHHHEEECCCCEEEEEEECCCC
LLLERQFRYPLRRDFIELPAGKIDPGEDDLTCAKRELEEETGYTASEWREVTTIYPCIGY
EEEEEHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCC
SDERLAFYLAEGLRDGTHGRDHDEFLEVFRLPFAEAMQWVRDGRICETKTVIGLFWLEKM
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEQGW
HHCCC
>Mature Secondary Structure
MENDSPDLVERELDSETVFKGRLMHVKRDRVQLPDGKESTREYIVHPGAVVVIPVFDNGD
CCCCCHHHHHHHCCCHHHHHHHHHEEHHCCCCCCCCCCHHHHEEECCCCEEEEEEECCCC
LLLERQFRYPLRRDFIELPAGKIDPGEDDLTCAKRELEEETGYTASEWREVTTIYPCIGY
EEEEEHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCC
SDERLAFYLAEGLRDGTHGRDHDEFLEVFRLPFAEAMQWVRDGRICETKTVIGLFWLEKM
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEQGW
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]